Rw1G040410

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002111
Physical Location & Seq
Reverse (-)
67696351 .. 67698734
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G040410.1

Sequence Viewer

Length: 1173 bp
ATGGATATGGGCTATGCGCAATCTATGCTAACTGAAAGCAGGAAAGGTAAGGAAAACCCTGCTGTCAGTTCTCCATCCAGGGAGGCCTATCAGAGGAAATTGGCAGAGGCTTTGAACATGAACCGCACTCGGATTCTTGCTTTCAAGAACAAGCCTCCGGCCCCGGTGGATCTGTACCCCAAAGATTTCTTCTCTTCTCAAACCCAAGATAAGCCAGCAAAGCCCAGGAGACACATTCCTCAAACCTCAGAGAGGACATTGGATGCTCCTGACCTTGTCGATGACTACTACCTCAATCTGTTGGATTGGGGAAGTGCCAATGTACTCGCAATTGCTCTCGCAAACACAGTTTACTTGTGGGATGCTAGCACTGGCTCTACTTCGGAATTGGAGACTTTTGATGATGAAACTGGACCTGTAACCAGTGTCAGTTGGGCTCCTGATGGACGCCACATTGCCGTTGGACTTAACAATTCCGAAGTACAGCTGTGGGACTCAACTGCTACTAAGCAGCTGAGGACTTTGAGAGGTTGCCACAGTTCGCGAGTTGGCTCATTGGCATGGAACAACAACATTCTTTCTACTGGAGGAATGGACGGCTGTATTGTCAACAATGATGTGAGAGTTAGATCCCACATTGTTGAGACCTACAGAGGGCATGAGCAAGAGGTTTGTGGACTTAAATGGTCAGCCTCAGGGCAGCAATTGGCGAGTGGAGGAAATGACAACCTACTGCATATATGGGACAGATCAGTTGCATCTTCACAGTCATCAACACAGTGGCTTCACAGGCTTGAAGATCATACAGCTGCTGTTAAAGCCCTTGCTTGGTGTCCATTCCAGAGCAACTTGCTTGCTTCCGGTGGAGGTGGGGGTGATCGGTGCATAAAGTTCTGGAACACCCACACAGGTGCATGCTTGAACTCAGTAGACACTGGGTCCAGGGAGTTGCTCAGCTCACACGGGTTTACACAGAACCAGCTGACTCTTTGGAAGTACCCATCCATGGTTAAAATGGCAGAACTTACAGGTCACACTTCCAGGGTTCTTTATATGGCTCAGACTGGTGATGAAACCCTGAGATTCTGGAATGTCTTTGGTGTCCCTGAGGTGGCTAAACCAGCTCCTAAAGCTCATTCTGAACCTTTTGCTCATATGAACCGTATCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

390

Amino Acids

43.16

Weight (kDa)

6.89

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 87 - 359 2.9e-102 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 97 - 191 1.2e-08 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 101 - 202 1.3e-09 WDR19 first beta-propeller
WDR55 PF24796 106 - 251 1.6e-09 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 107 - 189 2.1e-06 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 108 - 252 3.9e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 108 - 208 4.7e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 109 - 209 4.5e-10 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 109 - 193 5.2e-06 RIG second beta-propeller
Beta-prop_THOC3 PF25174 112 - 249 1.2e-19 THOC3 beta-propeller domain
WD40_Prp19 PF24814 113 - 190 6.4e-08 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 115 - 353 9.4e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 122 - 226 2.4e-10 TEP-1 second beta-propeller
ANAPC4_WD40 PF12894 134 - 190 2.4e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR3_2nd PF25172 141 - 260 1.7e-07 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 155 - 314 5.4e-06 SCAP Beta-propeller
WD40_Prp19 PF24814 189 - 349 7.6e-14 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 190 - 317 5e-17 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 212 - 315 3e-14 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 213 - 331 2e-09 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 214 - 249 8.4e-08 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 216 - 308 6.7e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 220 - 315 1.1e-18 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 18
AccI GTMKAC 1 cut(s) 930
AccII CGCG 1 cut(s) 544
AciI CCGC 1 cut(s) 124
AclWI GGATC 2 cut(s) 177, 624
AcyI GRCGYC 1 cut(s) 448
AfaI GTAC 4 cut(s) 176, 324, 483, 998
AfiI CCNNNNNNNGG 6 cut(s) 93, 252, 654, 828, 1006, 1111
AgsI TTSAA 4 cut(s) 115, 145, 797, 922
AhdI GACNNNNNGTC 1 cut(s) 937
AjnI CCWGG 4 cut(s) 77, 224, 941, 1040
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AleI CACNNNNGTG 1 cut(s) 909
AluBI AGCT 7 cut(s) 487, 514, 809, 957, 982, 1124, 1133
AluI AGCT 7 cut(s) 487, 514, 809, 957, 982, 1124, 1133
Alw26I GTCTC 3 cut(s) 223, 386, 638
AlwI GGATC 2 cut(s) 177, 624
AlwNI CAGNNNCTG 1 cut(s) 812
AoxI GGCC 2 cut(s) 84, 159
ApeKI GCWGC 3 cut(s) 511, 700, 809
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 3 cut(s) 160, 413, 939
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 887, 1079
AsuNHI GCTAGC 1 cut(s) 365
AvaII GGWCC 2 cut(s) 413, 939
AxyI CCTNAGG 2 cut(s) 694, 1107
BanII GRGCYC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 515
BbvI GCAGC 3 cut(s) 523, 712, 796
BccI CCATC 3 cut(s) 82, 437, 1009
BceAI ACGGC 2 cut(s) 443, 613
BciT130I CCWGG 4 cut(s) 79, 226, 943, 1042
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 3 cut(s) 223, 386, 638
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 1 cut(s) 649
BisI GCNGC 3 cut(s) 512, 701, 810
BlpI GCTNAGC 1 cut(s) 953
BlsI GCNGC 3 cut(s) 513, 702, 811
Bme1390I CCNGG 5 cut(s) 79, 164, 226, 943, 1042
Bme18I GGWCC 2 cut(s) 413, 939
BmeRI GACNNNNNGTC 1 cut(s) 937
BmgT120I GGNCC 3 cut(s) 160, 413, 939
BmiI GGNNCC 3 cut(s) 162, 438, 940
BmrFI CCNGG 5 cut(s) 79, 164, 226, 943, 1042
BmrI ACTGGG 1 cut(s) 945
BmsI GCATC 3 cut(s) 253, 352, 767
BmtI GCTAGC 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 945
BpmI CTGGAG 1 cut(s) 606
Bpu10I CCTNAGC 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 953
BpuMI CCSGG 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 638
BsaJI CCNNGG 6 cut(s) 78, 162, 224, 942, 1005, 1041
BsaWI WCCGGW 1 cut(s) 860
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 252, 654, 828, 1006, 1111
Bse1I ACTGG 6 cut(s) 376, 415, 423, 589, 940, 1069
Bse21I CCTNAGG 2 cut(s) 694, 1107
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 4 cut(s) 79, 226, 943, 1042
BseDI CCNNGG 6 cut(s) 78, 162, 224, 942, 1005, 1041
BseGI GGATG 4 cut(s) 74, 268, 367, 1001
BseLI CCNNNNNNNGG 6 cut(s) 93, 252, 654, 828, 1006, 1111
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 8 cut(s) 261, 506, 708, 939, 967, 1070, 1073, 1098
BseNI ACTGG 6 cut(s) 376, 415, 423, 589, 940, 1069
BseXI GCAGC 3 cut(s) 523, 712, 796
Bsh1236I CGCG 1 cut(s) 544
BshFI GGCC 2 cut(s) 86, 161
BsiSI CCGG 3 cut(s) 158, 164, 861
BslFI GGGAC 3 cut(s) 506, 758, 1088
BslI CCNNNNNNNGG 6 cut(s) 93, 252, 654, 828, 1006, 1111
BsmAI GTCTC 3 cut(s) 223, 386, 638
BsmFI GGGAC 3 cut(s) 506, 758, 1088
BsnI GGCC 2 cut(s) 86, 161
Bso31I GGTCTC 1 cut(s) 638
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 5 cut(s) 169, 629, 749, 799, 877
Bsp1720I GCTNAGC 1 cut(s) 953
Bsp19I CCATGG 1 cut(s) 1005
Bsp68I TCGCGA 1 cut(s) 544
BspACI CCGC 1 cut(s) 124
BspANI GGCC 2 cut(s) 86, 161
BspCNI CTCAG 8 cut(s) 260, 507, 707, 938, 966, 1071, 1072, 1099
BspFNI CGCG 1 cut(s) 544
BspLI GGNNCC 3 cut(s) 162, 438, 940
BspOI GCTAGC 1 cut(s) 369
BspPI GGATC 2 cut(s) 177, 624
BspTNI GGTCTC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 6 cut(s) 376, 415, 423, 589, 940, 1069
BssECI CCNNGG 6 cut(s) 78, 162, 224, 942, 1005, 1041
BssMI GATC 5 cut(s) 169, 629, 749, 799, 877
BssNI GRCGYC 1 cut(s) 448
BssT1I CCWWGG 1 cut(s) 1005
Bst2UI CCWGG 4 cut(s) 79, 226, 943, 1042
Bst4CI ACNGT 5 cut(s) 349, 539, 768, 780, 1163
Bst6I CTCTTC 1 cut(s) 199
BstACI GRCGYC 1 cut(s) 448
BstAPI GCANNNNNTGC 1 cut(s) 25
BstC8I GCNNGC 4 cut(s) 216, 367, 855, 916
BstDEI CTNAG 9 cut(s) 247, 507, 515, 694, 925, 953, 1059, 1079, 1107
BstDSI CCRYGG 1 cut(s) 1005
BstENI CCTNNNNNAGG 2 cut(s) 91, 250
BstF5I GGATG 4 cut(s) 74, 268, 367, 1001
BstFNI CGCG 1 cut(s) 544
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 5 cut(s) 172, 632, 752, 802, 880
BstMAI GTCTC 3 cut(s) 223, 386, 638
BstMBI GATC 5 cut(s) 169, 629, 749, 799, 877
BstMWI GCNNNNNNNGC 6 cut(s) 25, 220, 790, 818, 1121, 1130
BstNI CCWGG 4 cut(s) 79, 226, 943, 1042
BstNSI RCATGY 1 cut(s) 918
BstSCI CCNGG 5 cut(s) 77, 162, 224, 941, 1040
BstSFI CTRYAG 1 cut(s) 649
BstUI CGCG 1 cut(s) 544
BstV1I GCAGC 3 cut(s) 523, 712, 796
BstX2I RGATCY 2 cut(s) 169, 629
BstYI RGATCY 2 cut(s) 169, 629
Bsu36I CCTNAGG 2 cut(s) 694, 1107
BsuRI GGCC 2 cut(s) 86, 161
BtgI CCRYGG 1 cut(s) 1005
BtsCI GGATG 4 cut(s) 74, 268, 367, 1001
BtsIMutI CAGTG 4 cut(s) 369, 430, 785, 933
BtuMI TCGCGA 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 216, 367, 855, 916
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 3 cut(s) 160, 413, 939
CseI GACGC 1 cut(s) 456
Csp6I GTAC 4 cut(s) 175, 323, 482, 997
CviAII CATG 5 cut(s) 118, 561, 659, 915, 1006
CviQI GTAC 4 cut(s) 175, 323, 482, 997
DdeI CTNAG 9 cut(s) 247, 507, 515, 694, 925, 953, 1059, 1079, 1107
DpnI GATC 5 cut(s) 171, 631, 751, 801, 879
DpnII GATC 5 cut(s) 169, 629, 749, 799, 877
DriI GACNNNNNGTC 1 cut(s) 937
Eam1104I CTCTTC 1 cut(s) 199
Eam1105I GACNNNNNGTC 1 cut(s) 937
EarI CTCTTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 1005
Eco147I AGGCCT 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 439
Eco31I GGTCTC 1 cut(s) 638
Eco47I GGWCC 2 cut(s) 413, 939
Eco81I CCTNAGG 2 cut(s) 694, 1107
EcoNI CCTNNNNNAGG 2 cut(s) 91, 250
EcoRII CCWGG 4 cut(s) 77, 224, 941, 1040
EcoT14I CCWWGG 1 cut(s) 1005
EcoT38I GRGCYC 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 1005
FaeI CATG 5 cut(s) 121, 564, 662, 918, 1009
FaqI GGGAC 3 cut(s) 506, 758, 1088
FatI CATG 5 cut(s) 117, 560, 658, 914, 1005
FauNDI CATATG 1 cut(s) 1155
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 3 cut(s) 512, 701, 810
FokI GGATG 4 cut(s) 61, 275, 374, 988
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 3 cut(s) 512, 701, 810
FspBI CTAG 1 cut(s) 366
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 1 cut(s) 18
GluI GCNGC 3 cut(s) 512, 701, 810
GsuI CTGGAG 1 cut(s) 606
HaeIII GGCC 2 cut(s) 86, 161
HapII CCGG 3 cut(s) 158, 164, 861
HgaI GACGC 1 cut(s) 456
HhaI GCGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 448
Hin1II CATG 5 cut(s) 121, 564, 662, 918, 1009
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 4 cut(s) 133, 494, 985, 1083
HpaII CCGG 3 cut(s) 158, 164, 861
HphI GGTGA 2 cut(s) 887, 1079
Hpy166II GTNNAC 5 cut(s) 352, 610, 677, 931, 969
Hpy188I TCNGA 8 cut(s) 93, 132, 250, 385, 478, 1062, 1141, 1169
Hpy188III TCNNGA 7 cut(s) 145, 269, 440, 543, 841, 895, 1087
Hpy8I GTNNAC 5 cut(s) 352, 610, 677, 931, 969
HpyCH4III ACNGT 5 cut(s) 349, 539, 768, 780, 1163
HpyCH4V TGCA 4 cut(s) 736, 758, 885, 914
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 220, 790, 818, 1121, 1130
HpyF3I CTNAG 9 cut(s) 247, 507, 515, 694, 925, 953, 1059, 1079, 1107
Hsp92I GRCGYC 1 cut(s) 448
Hsp92II CATG 5 cut(s) 121, 564, 662, 918, 1009
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 5 cut(s) 169, 629, 749, 799, 877
LmnI GCTCC 3 cut(s) 271, 442, 1129
Lsp1109I GCAGC 3 cut(s) 523, 712, 796
LweI GCATC 3 cut(s) 253, 352, 767
MaeI CTAG 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 418, 1031
MalI GATC 5 cut(s) 171, 631, 751, 801, 879
MboI GATC 5 cut(s) 169, 629, 749, 799, 877
MboII GAAGA 4 cut(s) 181, 186, 753, 809
MfeI CAATTG 2 cut(s) 330, 704
MflI RGATCY 2 cut(s) 169, 629
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 5 cut(s) 98, 330, 386, 472, 704
MlyI GAGTC 2 cut(s) 488, 979
MmeI TCCRAC 2 cut(s) 282, 442
MseI TTAA 4 cut(s) 468, 681, 816, 1011
MslI CAYNNNNRTG 2 cut(s) 559, 909
MspA1I CMGCKG 4 cut(s) 487, 514, 809, 982
MspI CCGG 3 cut(s) 158, 164, 861
MspR9I CCNGG 5 cut(s) 79, 164, 226, 943, 1042
MunI CAATTG 2 cut(s) 330, 704
MvaI CCWGG 4 cut(s) 79, 226, 943, 1042
MvnI CGCG 1 cut(s) 544
MwoI GCNNNNNNNGC 6 cut(s) 25, 220, 790, 818, 1121, 1130
NciI CCSGG 1 cut(s) 164
NcoI CCATGG 1 cut(s) 1005
NdeI CATATG 1 cut(s) 1155
NdeII GATC 5 cut(s) 169, 629, 749, 799, 877
NheI GCTAGC 1 cut(s) 365
NlaIII CATG 5 cut(s) 121, 564, 662, 918, 1009
NlaIV GGNNCC 3 cut(s) 162, 438, 940
NmuCI GTSAC 1 cut(s) 1031
NruI TCGCGA 1 cut(s) 544
NsbI TGCGCA 1 cut(s) 18
NspI RCATGY 1 cut(s) 918
OliI CACNNNNGTG 1 cut(s) 909
PaeI GCATGC 1 cut(s) 918
PceI AGGCCT 1 cut(s) 86
PfeI GAWTC 2 cut(s) 133, 1083
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 3 cut(s) 513, 702, 811
PleI GAGTC 2 cut(s) 488, 979
PpsI GAGTC 2 cut(s) 488, 979
Psp6I CCWGG 4 cut(s) 77, 224, 941, 1040
PspGI CCWGG 4 cut(s) 77, 224, 941, 1040
PspN4I GGNNCC 3 cut(s) 162, 438, 940
PspPI GGNCC 3 cut(s) 160, 413, 939
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 2 cut(s) 169, 629
PsyI GACNNNGTC 1 cut(s) 275
PvuII CAGCTG 4 cut(s) 487, 514, 809, 982
RruI TCGCGA 1 cut(s) 544
RsaI GTAC 4 cut(s) 176, 324, 483, 998
RsaNI GTAC 4 cut(s) 175, 323, 482, 997
RseI CAYNNNNRTG 2 cut(s) 559, 909
SaqAI TTAA 4 cut(s) 468, 681, 816, 1011
SatI GCNGC 3 cut(s) 512, 701, 810
Sau3AI GATC 5 cut(s) 169, 629, 749, 799, 877
Sau96I GGNCC 3 cut(s) 160, 413, 939
SchI GAGTC 2 cut(s) 488, 979
ScrFI CCNGG 5 cut(s) 79, 164, 226, 943, 1042
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 3 cut(s) 253, 352, 767
SfcI CTRYAG 1 cut(s) 649
SinI GGWCC 2 cut(s) 413, 939
SmiMI CAYNNNNRTG 2 cut(s) 559, 909
SphI GCATGC 1 cut(s) 918
Sse9I AATT 5 cut(s) 98, 330, 386, 472, 704
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 1 cut(s) 124
SspMI CTAG 1 cut(s) 366
StuI AGGCCT 1 cut(s) 86
StyD4I CCNGG 5 cut(s) 77, 162, 224, 941, 1040
StyI CCWWGG 1 cut(s) 1005
TaaI ACNGT 5 cut(s) 349, 539, 768, 780, 1163
TaqI TCGA 1 cut(s) 279
TasI AATT 5 cut(s) 98, 330, 386, 472, 704
TatI WGTACW 2 cut(s) 322, 481
TfiI GAWTC 2 cut(s) 133, 1083
Tru1I TTAA 4 cut(s) 468, 681, 816, 1011
Tru9I TTAA 4 cut(s) 468, 681, 816, 1011
TscAI CASTG 4 cut(s) 376, 430, 785, 940
TseFI GTSAC 1 cut(s) 1031
TseI GCWGC 3 cut(s) 511, 700, 809
Tsp45I GTSAC 1 cut(s) 1031
TspDTI ATGAA 4 cut(s) 134, 420, 1086, 1172
TspRI CASTG 4 cut(s) 376, 430, 785, 940
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 413, 939
XagI CCTNNNNNAGG 2 cut(s) 91, 250
XceI RCATGY 1 cut(s) 918
XcmI CCANNNNNNNNNTGG 2 cut(s) 458, 1012
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.