pycom01g24180

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002111
Physical Location & Seq
Forward (+)
21184433 .. 21186697
2265 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g24180.3

Sequence Viewer

Length: 1362 bp
ATGGACGCAGGATCAACGAGTTCTCCATCTAACTTGAAGTCGCAGTCTAGGTGCCCTCTTCAAGAGCAGTTTCTTCAGAGAAAGAATTCTCGGGAAAATTTGGACAGGTTCATACCAAACAGGTCAGCAATGGATTTTGATTTCGCAAACATGATGTTAACTGAAGGTAGGAAAGGTAAGGAAAACCCAGCCGTCTCTTCCCCATCCAGAGAGGCCTATCGGAAGCAGTTGGCAGAGGCAATGAACATGAACCGGAGCAGAATTCTTGCATTCAAGAACAAGCCACCTGCCCCTGTTGAGATGTTCCCACGCGAGTTCTCTTCTCTGCAACAGGATAAACCAGCAAAGCCCCGAAGGCACATTCCTCAAACTTCAGAGAAGACATTGGATGCTCCAGACCTTGTGGATGATTACTACCTCAATTTGTTGGATTGGGGAAGCTGCAATGTCCTCGCAATAGCTCTTGCAAGCACAGTTTATTTGTGGGATGCTACCAATGGTTCTACTTCGGAACTGGTCACATTTGAGGATGGAGTTGGATTTGTTACCAGCGTCAGTTGGGCTCCTGATGGGCGCCACATTGCCATTGGACTGGACAATTCTGAAGTACAGTTATGGGATTCGGTTGCTAAAAAGCAGCTGAGAACTTTGAGAGGTTGCCACAGATCACGAGTAGGCTCTTTGGCGTGGAACAACCACATTCTTTCAACTGGAGGGATGGACGGTTGCATTGTTAACAATGATGTAAGAATTAGATCACACATTGTTGAGACATACAGAGGACATGAGCAAGAGGTTTGTGGACTGAAGTGGTCAGCCTCAGGCCAGCAATTGGCTAGTGGAGGAAACGACAACCTTCTCCATATATGGGACAGATCGGTGGCATCTTCAAATTCACCAACTCAGTGGCTTCACAGGCTTGAAGATCACACAGCTGCAGTGAAAGCCCTTGCTTGGTGTCCTTTCCAGGGCAATTTATTGGCTTCTGGTGGAGGCGGGGGTGACCGGTGCATAAAGTTTTGGAACACTCACACAGGTGCATGCTTGAACTCAGTCGACACTGGCTCTCAGGTCTGTGCTTTGCTGTGGAACAAGAATGAAAGGGAACTGCTTAGCTCACATGGGTTTACTCAGAATCAGCTCACTCTTTGGAAATACCCTTCGATGGCTAAAATTGCCGAGCTCACAGGCCATACTTCTAGAGTTCTTTATATGGCTCAGAGCCCCGATGGTTGCACGGTCGCATCAGCAGCTGGTGATGAAACACTCAGATTCTGGAACGTTTTTGGGGTCCCCGAGGTTGCTAAACCAGCTCCCAAAGCTAACCCTGAGCCCTTTGCTCATCTGAACCGTATTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

454

Amino Acids

50.18

Weight (kDa)

8.28

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 295
AccB1I GGYRCC 2 cut(s) 51, 573
AccB7I CCANNNNNTGG 1 cut(s) 832
AccI GTMKAC 1 cut(s) 1056
AccII CGCG 1 cut(s) 312
AciI CCGC 1 cut(s) 996
AclI AACGTT 1 cut(s) 1281
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 4 cut(s) 85, 97, 261, 892
AcuI CTGAAG 5 cut(s) 59, 183, 357, 624, 827
AcyI GRCGYC 1 cut(s) 574
AfaI GTAC 1 cut(s) 609
AfiI CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
AgeI ACCGGT 1 cut(s) 1005
AgsI TTSAA 7 cut(s) 37, 62, 274, 708, 891, 923, 1048
AjnI CCWGG 1 cut(s) 966
AleI CACNNNNGTG 1 cut(s) 1035
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 2 cut(s) 199, 764
AlwI GGATC 1 cut(s) 19
AlwNI CAGNNNCTG 2 cut(s) 1253, 1275
Ama87I CYCGRG 2 cut(s) 90, 1295
AoxI GGCC 3 cut(s) 213, 823, 1189
ApeKI GCWGC 4 cut(s) 441, 637, 935, 1250
ApoI RAATTY 4 cut(s) 85, 97, 261, 892
AsiGI ACCGGT 1 cut(s) 1005
Asp700I GAANNNNTTC 1 cut(s) 85
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 1 cut(s) 1291
AsuHPI GGTGA 3 cut(s) 888, 1013, 1268
AvaI CYCGRG 2 cut(s) 90, 1295
AvaII GGWCC 1 cut(s) 1291
AxyI CCTNAGG 1 cut(s) 820
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 2 cut(s) 51, 573
BanII GRGCYC 4 cut(s) 565, 1185, 1226, 1335
BauI CACGAG 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 4 cut(s) 428, 649, 922, 1262
BccI CCATC 7 cut(s) 34, 211, 524, 563, 712, 1159, 1223
BceAI ACGGC 1 cut(s) 176
BcgI CGANNNNNNTGC 2 cut(s) 433, 467
BciT130I CCWGG 1 cut(s) 968
BcoDI GTCTC 2 cut(s) 199, 764
BfaI CTAG 3 cut(s) 48, 837, 1200
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 1 cut(s) 577
BfuAI ACCTGC 1 cut(s) 295
BglI GCCNNNNNGGC 1 cut(s) 355
BisI GCNGC 4 cut(s) 442, 638, 936, 1251
BlpI GCTNAGC 1 cut(s) 1112
BlsI GCNGC 4 cut(s) 443, 639, 937, 1252
Bme1390I CCNGG 1 cut(s) 968
Bme18I GGWCC 1 cut(s) 1291
BmeT110I CYCGRG 2 cut(s) 90, 1295
BmgT120I GGNCC 1 cut(s) 1291
BmiI GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
BmrFI CCNGG 1 cut(s) 968
BmsI GCATC 4 cut(s) 379, 478, 893, 1253
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 2 cut(s) 378, 732
Bpu10I CCTNAGC 1 cut(s) 1329
Bpu1102I GCTNAGC 1 cut(s) 1112
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 967, 1296
BsaWI WCCGGW 2 cut(s) 252, 1005
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
Bse118I RCCGGY 1 cut(s) 1005
Bse1I ACTGG 4 cut(s) 519, 597, 715, 1066
Bse21I CCTNAGG 1 cut(s) 820
Bse3DI GCAATG 4 cut(s) 135, 246, 451, 579
BseBI CCWGG 1 cut(s) 968
BseDI CCNNGG 2 cut(s) 967, 1296
BseGI GGATG 6 cut(s) 203, 394, 412, 493, 535, 723
BseLI CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
BseMI GCAATG 4 cut(s) 135, 246, 451, 579
BseMII CTCAG 9 cut(s) 632, 834, 917, 1065, 1082, 1145, 1232, 1282, 1320
BseNI ACTGG 4 cut(s) 519, 597, 715, 1066
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 4 cut(s) 428, 649, 922, 1262
BseYI CCCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 1 cut(s) 1242
BshFI GGCC 3 cut(s) 215, 825, 1191
BshNI GGYRCC 2 cut(s) 51, 573
BshTI ACCGGT 1 cut(s) 1005
BsiEI CGRYCG 1 cut(s) 1242
BsiHKAI GWGCWC 1 cut(s) 1185
BsiHKCI CYCGRG 2 cut(s) 90, 1295
BsiSI CCGG 2 cut(s) 253, 1006
BslFI GGGAC 2 cut(s) 884, 1277
BslI CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
BsmAI GTCTC 2 cut(s) 199, 764
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 2 cut(s) 884, 1277
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 3 cut(s) 215, 825, 1191
BsoBI CYCGRG 2 cut(s) 90, 1295
Bsp1286I GDGCHC 5 cut(s) 56, 565, 1185, 1226, 1335
Bsp143I GATC 5 cut(s) 11, 665, 755, 875, 925
Bsp1720I GCTNAGC 1 cut(s) 1112
BspACI CCGC 1 cut(s) 996
BspANI GGCC 3 cut(s) 215, 825, 1191
BspCNI CTCAG 9 cut(s) 633, 833, 916, 1064, 1081, 1144, 1231, 1281, 1321
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
BspMAI CTGCAG 1 cut(s) 940
BspMI ACCTGC 1 cut(s) 295
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 2 cut(s) 51, 573
BsrDI GCAATG 4 cut(s) 135, 246, 451, 579
BsrFI RCCGGY 1 cut(s) 1005
BsrI ACTGG 4 cut(s) 519, 597, 715, 1066
BssAI RCCGGY 1 cut(s) 1005
BssECI CCNNGG 2 cut(s) 967, 1296
BssMI GATC 5 cut(s) 11, 665, 755, 875, 925
BssNI GRCGYC 1 cut(s) 574
BssSI CACGAG 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 1 cut(s) 968
Bst4CI ACNGT 5 cut(s) 475, 612, 725, 1240, 1352
Bst6I CTCTTC 3 cut(s) 63, 202, 325
BstACI GRCGYC 1 cut(s) 574
BstC8I GCNNGC 3 cut(s) 469, 827, 1042
BstEII GGTNACC 1 cut(s) 1001
BstF5I GGATG 6 cut(s) 203, 394, 412, 493, 535, 723
BstFNI CGCG 1 cut(s) 312
BstH2I RGCGCY 1 cut(s) 577
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 5 cut(s) 14, 668, 758, 878, 928
BstMAI GTCTC 2 cut(s) 199, 764
BstMBI GATC 5 cut(s) 11, 665, 755, 875, 925
BstMCI CGRYCG 1 cut(s) 1242
BstMWI GCNNNNNNNGC 7 cut(s) 355, 916, 944, 1175, 1250, 1310, 1319
BstNI CCWGG 1 cut(s) 968
BstNSI RCATGY 1 cut(s) 1044
BstPI GGTNACC 1 cut(s) 1001
BstSCI CCNGG 1 cut(s) 966
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 428, 649, 922, 1262
BstV2I GAAGAC 1 cut(s) 386
BstXI CCANNNNNNTGG 2 cut(s) 592, 906
Bsu36I CCTNAGG 1 cut(s) 820
BsuRI GGCC 3 cut(s) 215, 825, 1191
BtsCI GGATG 6 cut(s) 203, 394, 412, 493, 535, 723
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 3 cut(s) 911, 945, 1059
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 3 cut(s) 469, 827, 1042
CaiI CAGNNNCTG 2 cut(s) 1253, 1275
CfoI GCGC 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 1005
Cfr13I GGNCC 1 cut(s) 1291
CseI GACGC 2 cut(s) 14, 541
Csp6I GTAC 1 cut(s) 608
CspAI ACCGGT 1 cut(s) 1005
CviAII CATG 5 cut(s) 151, 247, 785, 1041, 1121
CviQI GTAC 1 cut(s) 608
DinI GGCGCC 1 cut(s) 575
DpnI GATC 5 cut(s) 13, 667, 757, 877, 927
DpnII GATC 5 cut(s) 11, 665, 755, 875, 925
Eam1104I CTCTTC 3 cut(s) 63, 202, 325
EarI CTCTTC 3 cut(s) 63, 202, 325
Ecl136II GAGCTC 1 cut(s) 1183
Eco147I AGGCCT 1 cut(s) 215
Eco24I GRGCYC 4 cut(s) 565, 1185, 1226, 1335
Eco47I GGWCC 1 cut(s) 1291
Eco53kI GAGCTC 1 cut(s) 1183
Eco57I CTGAAG 5 cut(s) 59, 183, 357, 624, 827
Eco81I CCTNAGG 1 cut(s) 820
Eco88I CYCGRG 2 cut(s) 90, 1295
Eco91I GGTNACC 1 cut(s) 1001
EcoICRI GAGCTC 1 cut(s) 1183
EcoO109I RGGNCCY 1 cut(s) 1291
EcoO65I GGTNACC 1 cut(s) 1001
EcoRI GAATTC 2 cut(s) 85, 261
EcoRII CCWGG 1 cut(s) 966
EcoT38I GRGCYC 4 cut(s) 565, 1185, 1226, 1335
EgeI GGCGCC 1 cut(s) 575
EheI GGCGCC 1 cut(s) 575
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 5 cut(s) 154, 250, 788, 1044, 1124
FaqI GGGAC 2 cut(s) 884, 1277
FatI CATG 5 cut(s) 150, 246, 784, 1040, 1120
FauI CCCGC 1 cut(s) 989
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 4 cut(s) 442, 638, 936, 1251
FokI GGATG 6 cut(s) 190, 401, 419, 500, 542, 730
FriOI GRGCYC 4 cut(s) 565, 1185, 1226, 1335
Fsp4HI GCNGC 4 cut(s) 442, 638, 936, 1251
FspBI CTAG 3 cut(s) 48, 837, 1200
GlaI GCGC 1 cut(s) 575
GluI GCNGC 4 cut(s) 442, 638, 936, 1251
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 2 cut(s) 378, 732
HaeII RGCGCY 1 cut(s) 577
HaeIII GGCC 3 cut(s) 215, 825, 1191
HapII CCGG 2 cut(s) 253, 1006
HgaI GACGC 2 cut(s) 14, 541
HhaI GCGC 1 cut(s) 576
Hin1I GRCGYC 1 cut(s) 574
Hin1II CATG 5 cut(s) 154, 250, 788, 1044, 1124
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 3 cut(s) 159, 736, 1057
HindII GTYRAC 3 cut(s) 159, 736, 1057
HinfI GANTC 3 cut(s) 620, 1135, 1272
HpaI GTTAAC 2 cut(s) 159, 736
HpaII CCGG 2 cut(s) 253, 1006
HphI GGTGA 3 cut(s) 888, 1013, 1268
Hpy166II GTNNAC 5 cut(s) 159, 736, 803, 1057, 1128
Hpy188I TCNGA 9 cut(s) 78, 222, 376, 511, 604, 1134, 1221, 1271, 1347
Hpy188III TCNNGA 9 cut(s) 62, 92, 207, 274, 395, 566, 669, 1200, 1276
Hpy8I GTNNAC 5 cut(s) 159, 736, 803, 1057, 1128
HpyAV CCTTC 4 cut(s) 158, 348, 866, 1170
HpyCH4III ACNGT 5 cut(s) 475, 612, 725, 1240, 1352
HpyCH4IV ACGT 1 cut(s) 1281
HpyCH4V TGCA 9 cut(s) 269, 328, 444, 467, 729, 938, 1011, 1040, 1236
HpyF10VI GCNNNNNNNGC 7 cut(s) 355, 916, 944, 1175, 1250, 1310, 1319
HpySE526I ACGT 1 cut(s) 1281
Hsp92I GRCGYC 1 cut(s) 574
Hsp92II CATG 5 cut(s) 154, 250, 788, 1044, 1124
HspAI GCGC 1 cut(s) 574
KasI GGCGCC 1 cut(s) 573
KflI GGGWCCC 1 cut(s) 1291
KspAI GTTAAC 2 cut(s) 159, 736
Kzo9I GATC 5 cut(s) 11, 665, 755, 875, 925
LmnI GCTCC 4 cut(s) 255, 397, 568, 1318
Lsp1109I GCAGC 4 cut(s) 428, 649, 922, 1262
LweI GCATC 4 cut(s) 379, 478, 893, 1253
MaeI CTAG 3 cut(s) 48, 837, 1200
MaeII ACGT 1 cut(s) 1281
MaeIII GTNAC 3 cut(s) 517, 544, 1001
MalI GATC 5 cut(s) 13, 667, 757, 877, 927
MboI GATC 5 cut(s) 11, 665, 755, 875, 925
MboII GAAGA 7 cut(s) 50, 65, 189, 312, 391, 879, 935
MfeI CAATTG 1 cut(s) 830
MhlI GDGCHC 5 cut(s) 56, 565, 1185, 1226, 1335
Mly113I GGCGCC 1 cut(s) 574
MmeI TCCRAC 2 cut(s) 408, 517
MroXI GAANNNNTTC 1 cut(s) 85
MseI TTAA 2 cut(s) 158, 735
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 3 cut(s) 640, 935, 1253
MspI CCGG 2 cut(s) 253, 1006
MspR9I CCNGG 1 cut(s) 968
MunI CAATTG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 1 cut(s) 968
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 7 cut(s) 355, 916, 944, 1175, 1250, 1310, 1319
NarI GGCGCC 1 cut(s) 574
NdeII GATC 5 cut(s) 11, 665, 755, 875, 925
NlaIII CATG 5 cut(s) 154, 250, 788, 1044, 1124
NlaIV GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
NmeAIII GCCGAG 1 cut(s) 1204
NmuCI GTSAC 2 cut(s) 517, 1001
NspI RCATGY 1 cut(s) 1044
OliI CACNNNNGTG 1 cut(s) 1035
PaeI GCATGC 1 cut(s) 1044
PaqCI CACCTGC 1 cut(s) 295
PceI AGGCCT 1 cut(s) 215
PctI GAATGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 620, 1135, 1272
PflMI CCANNNNNTGG 1 cut(s) 832
PinAI ACCGGT 1 cut(s) 1005
PkrI GCNGC 4 cut(s) 443, 639, 937, 1252
PluTI GGCGCC 1 cut(s) 577
PpuMI RGGWCCY 1 cut(s) 1291
Psp124BI GAGCTC 1 cut(s) 1185
Psp1406I AACGTT 1 cut(s) 1281
Psp5II RGGWCCY 1 cut(s) 1291
Psp6I CCWGG 1 cut(s) 966
PspEI GGTNACC 1 cut(s) 1001
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 966
PspN4I GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
PspPI GGNCC 1 cut(s) 1291
PspPPI RGGWCCY 1 cut(s) 1291
PstI CTGCAG 1 cut(s) 940
PstNI CAGNNNCTG 2 cut(s) 1253, 1275
PvuII CAGCTG 3 cut(s) 640, 935, 1253
RsaI GTAC 1 cut(s) 609
RsaNI GTAC 1 cut(s) 608
RseI CAYNNNNRTG 1 cut(s) 1035
SacI GAGCTC 1 cut(s) 1185
SalI GTCGAC 1 cut(s) 1055
SaqAI TTAA 2 cut(s) 158, 735
SatI GCNGC 4 cut(s) 442, 638, 936, 1251
Sau3AI GATC 5 cut(s) 11, 665, 755, 875, 925
Sau96I GGNCC 1 cut(s) 1291
ScrFI CCNGG 1 cut(s) 968
SduI GDGCHC 5 cut(s) 56, 565, 1185, 1226, 1335
SfaNI GCATC 4 cut(s) 379, 478, 893, 1253
SfcI CTRYAG 1 cut(s) 936
SfoI GGCGCC 1 cut(s) 575
SinI GGWCC 1 cut(s) 1291
SmiMI CAYNNNNRTG 1 cut(s) 1035
SphI GCATGC 1 cut(s) 1044
SseBI AGGCCT 1 cut(s) 215
SsiI CCGC 1 cut(s) 996
SspDI GGCGCC 1 cut(s) 573
SspMI CTAG 3 cut(s) 48, 837, 1200
SstI GAGCTC 1 cut(s) 1185
StuI AGGCCT 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 966
TaaI ACNGT 5 cut(s) 475, 612, 725, 1240, 1352
TaiI ACGT 1 cut(s) 1284
TaqI TCGA 2 cut(s) 1056, 1163
TatI WGTACW 1 cut(s) 607
TfiI GAWTC 3 cut(s) 620, 1135, 1272
Tru1I TTAA 2 cut(s) 158, 735
Tru9I TTAA 2 cut(s) 158, 735
TscAI CASTG 3 cut(s) 911, 945, 1066
TseFI GTSAC 2 cut(s) 517, 1001
TseI GCWGC 4 cut(s) 441, 637, 935, 1250
Tsp45I GTSAC 2 cut(s) 517, 1001
TspDTI ATGAA 5 cut(s) 100, 257, 263, 1113, 1275
TspRI CASTG 3 cut(s) 911, 945, 1066
Van91I CCANNNNNTGG 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 1291
XapI RAATTY 4 cut(s) 85, 97, 261, 892
XbaI TCTAGA 1 cut(s) 1199
XceI RCATGY 1 cut(s) 1044
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 1056
XmnI GAANNNNTTC 1 cut(s) 85
XspI CTAG 3 cut(s) 48, 837, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.