RLG00000023716

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002111
Physical Location & Seq
Reverse (-)
27398215 .. 27402334
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023716

Sequence Viewer

Length: 867 bp
ATGGAGCGGCTGGGGGAGGTAGGGCGGTCGTGGAGAGAGAGCCGACGGGGAGGATATGGTGAGGCTCACGTACGGGGGAGAGGCGACGTGTGTGAAGAAACAGGCGGCGGAGGGGGTGGAGAAGCTGGAAGTGGAGGAGGCGAAGCCGGTGGAGGAGGAGTGGGAGGTGCTATGGATTCATCAAGAAAGGTCAAGTCCCTTTACCCAATTCAAGAACCGCTCTTTCCAAGAAAGCGTAGTCGAGAAAACTTGGATAGGTTCATCCCTGATAGGGAGTCTATGGACATTGGTTATGCAAATGCCGTGCTCACAGAGAGGAGAACAGGCAAAGAGAACCCGGTTGAGAGCTCCGCATCGAGTGTGGCATACCAGAAGCTATTGGCTGAGACACTCAACATGAACCGGAGAATTCTTACATTCAAGAGCAAGCCTCCTGCCCCAATTGAACCAATTCCTAGGGCATTGCTTTCACCTCCAAGTCATAAGGCCAAATCCAGGCCCCGGAGACACATTTCTCAGACTGCAGAGAAGATATTGGATGCTCCCGATCTCTCTGATGATTTCTACTTGAATTTACTGGACTGGGGCAGCAACAACATCCTTGCAATAGCTCTTGAAAGCACGGTGTACCTATGGGATGCTTCAAATAGCTCCACCTCAGAGCTTGTCACAGTTTCTGATGATGATGGTCCTGTTACAAGCATTAGCTGGGCTGCTGATGGGAGGCATATTGCTATTGGGCTAAACAATTCTTGTGTCGAATTGTGGGATTCTATTTCTGTTAAACTGGTAATGGGAAGTGACTATATCACCAAACAAGCTCCATATAGCGTACATCTCCAAAATGCTCAGCTATTCCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

289

Amino Acids

31.23

Weight (kDa)

6.02

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 179 - 264 6.2e-28 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 200 - 262 3.8e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 7, 220
AciI CCGC 6 cut(s) 7, 25, 105, 108, 218, 351
AcsI RAATTY 2 cut(s) 408, 571
AfaI GTAC 3 cut(s) 72, 629, 834
AfiI CCNNNNNNNGG 3 cut(s) 271, 495, 501
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 6 cut(s) 212, 421, 446, 571, 617, 645
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 1 cut(s) 494
Alw21I GWGCWC 2 cut(s) 309, 350
Alw26I GTCTC 2 cut(s) 380, 499
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 2 cut(s) 486, 497
ApeKI GCWGC 2 cut(s) 588, 713
ApoI RAATTY 2 cut(s) 408, 571
Asp700I GAANNNNTTC 1 cut(s) 450
AspA2I CCTAGG 1 cut(s) 455
AspS9I GGNCC 2 cut(s) 498, 689
AsuC2I CCSGG 2 cut(s) 338, 502
AsuHPI GGTGA 3 cut(s) 71, 462, 802
AvaII GGWCC 1 cut(s) 689
AvrII CCTAGG 1 cut(s) 455
BanII GRGCYC 1 cut(s) 350
Bbv12I GWGCWC 2 cut(s) 309, 350
BbvI GCAGC 2 cut(s) 600, 700
BccI CCATC 2 cut(s) 680, 713
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 1 cut(s) 496
BcnI CCSGG 2 cut(s) 338, 502
BcoDI GTCTC 2 cut(s) 380, 499
BfaI CTAG 1 cut(s) 456
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 4 cut(s) 8, 106, 589, 714
BlnI CCTAGG 1 cut(s) 455
BlpI GCTNAGC 1 cut(s) 849
BlsI GCNGC 4 cut(s) 9, 107, 590, 715
Bme1390I CCNGG 3 cut(s) 338, 496, 502
Bme18I GGWCC 1 cut(s) 689
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 498, 689
BmiI GGNNCC 1 cut(s) 500
BmrFI CCNGG 3 cut(s) 338, 496, 502
BmrI ACTGGG 1 cut(s) 592
BmsI GCATC 3 cut(s) 362, 529, 628
BmuI ACTGGG 1 cut(s) 592
BplI GAGNNNNNCTC 2 cut(s) 415, 447
Bpu1102I GCTNAGC 1 cut(s) 849
BpuMI CCSGG 2 cut(s) 338, 502
BsaAI YACGTR 1 cut(s) 70
BsaJI CCNNGG 2 cut(s) 455, 500
BsaWI WCCGGW 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 495, 501
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 3 cut(s) 582, 587, 792
Bse3DI GCAATG 1 cut(s) 461
BseBI CCWGG 1 cut(s) 496
BseDI CCNNGG 2 cut(s) 455, 500
BseGI GGATG 4 cut(s) 261, 544, 597, 643
BseLI CCNNNNNNNGG 3 cut(s) 271, 495, 501
BseMI GCAATG 1 cut(s) 461
BseMII CTCAG 4 cut(s) 375, 530, 672, 863
BseNI ACTGG 3 cut(s) 582, 587, 792
BseRI GAGGAG 4 cut(s) 150, 168, 171, 331
BseXI GCAGC 2 cut(s) 600, 700
BseYI CCCAGC 2 cut(s) 10, 708
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 2 cut(s) 488, 499
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 2 cut(s) 309, 350
BsiSI CCGG 4 cut(s) 147, 338, 403, 502
BsiWI CGTACG 1 cut(s) 70
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 3 cut(s) 271, 495, 501
BsmAI GTCTC 2 cut(s) 380, 499
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 2 cut(s) 488, 499
Bsp1286I GDGCHC 2 cut(s) 309, 350
Bsp143I GATC 1 cut(s) 547
Bsp1720I GCTNAGC 1 cut(s) 849
BspACI CCGC 6 cut(s) 7, 25, 105, 108, 218, 351
BspANI GGCC 2 cut(s) 488, 499
BspCNI CTCAG 4 cut(s) 376, 529, 671, 862
BspLI GGNNCC 1 cut(s) 500
BspMAI CTGCAG 1 cut(s) 526
BsrBI CCGCTC 2 cut(s) 7, 220
BsrDI GCAATG 1 cut(s) 461
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 3 cut(s) 582, 587, 792
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 455, 500
BssMI GATC 1 cut(s) 547
BssT1I CCWWGG 1 cut(s) 455
Bst2UI CCWGG 1 cut(s) 496
Bst4CI ACNGT 2 cut(s) 625, 673
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 428
BstDEI CTNAG 4 cut(s) 384, 516, 658, 849
BstF5I GGATG 4 cut(s) 261, 544, 597, 643
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 2 cut(s) 380, 499
BstMBI GATC 1 cut(s) 547
BstMCI CGRYCG 1 cut(s) 29
BstNI CCWGG 1 cut(s) 496
BstSCI CCNGG 3 cut(s) 336, 494, 500
BstSFI CTRYAG 1 cut(s) 522
BstV1I GCAGC 2 cut(s) 600, 700
BsuRI GGCC 2 cut(s) 488, 499
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 4 cut(s) 261, 544, 597, 643
Cac8I GCNNGC 1 cut(s) 428
CaiI CAGNNNCTG 1 cut(s) 677
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 498, 689
Csp6I GTAC 3 cut(s) 71, 628, 833
CviAII CATG 1 cut(s) 397
CviQI GTAC 3 cut(s) 71, 628, 833
DdeI CTNAG 4 cut(s) 384, 516, 658, 849
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
EciI GGCGGA 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 689
Eco53kI GAGCTC 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 1 cut(s) 494
EcoT14I CCWWGG 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 1 cut(s) 400
FaqI GGGAC 1 cut(s) 181
FatI CATG 1 cut(s) 396
Fnu4HI GCNGC 4 cut(s) 8, 106, 589, 714
FokI GGATG 4 cut(s) 248, 551, 584, 650
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 4 cut(s) 8, 106, 589, 714
FspBI CTAG 1 cut(s) 456
GluI GCNGC 4 cut(s) 8, 106, 589, 714
GsaI CCCAGC 2 cut(s) 14, 712
HaeIII GGCC 2 cut(s) 488, 499
HapII CCGG 4 cut(s) 147, 338, 403, 502
Hin1II CATG 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 3 cut(s) 176, 275, 770
HpaII CCGG 4 cut(s) 147, 338, 403, 502
HphI GGTGA 3 cut(s) 71, 462, 802
Hpy166II GTNNAC 1 cut(s) 628
Hpy188I TCNGA 4 cut(s) 519, 556, 661, 679
Hpy188III TCNNGA 6 cut(s) 183, 212, 242, 421, 545, 614
Hpy8I GTNNAC 1 cut(s) 628
Hpy99I CGWCG 2 cut(s) 48, 89
HpyCH4III ACNGT 2 cut(s) 625, 673
HpyCH4IV ACGT 2 cut(s) 69, 87
HpyCH4V TGCA 3 cut(s) 296, 524, 605
HpyF3I CTNAG 4 cut(s) 384, 516, 658, 849
HpySE526I ACGT 2 cut(s) 69, 87
Hsp92II CATG 1 cut(s) 400
Kzo9I GATC 1 cut(s) 547
LmnI GCTCC 5 cut(s) 4, 353, 547, 656, 826
Lsp1109I GCAGC 2 cut(s) 600, 700
LweI GCATC 3 cut(s) 362, 529, 628
MaeI CTAG 1 cut(s) 456
MaeII ACGT 2 cut(s) 69, 87
MaeIII GTNAC 3 cut(s) 667, 694, 800
MalI GATC 1 cut(s) 549
MbiI CCGCTC 2 cut(s) 7, 220
MboI GATC 1 cut(s) 547
MboII GAAGA 2 cut(s) 107, 541
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 2 cut(s) 309, 350
MluCI AATT 7 cut(s) 207, 408, 441, 450, 571, 748, 761
MlyI GAGTC 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 2 cut(s) 783, 865
MspI CCGG 4 cut(s) 147, 338, 403, 502
MspR9I CCNGG 3 cut(s) 338, 496, 502
MunI CAATTG 1 cut(s) 441
MvaI CCWGG 1 cut(s) 496
NciI CCSGG 2 cut(s) 338, 502
NdeII GATC 1 cut(s) 547
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 500
NmuCI GTSAC 2 cut(s) 667, 800
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 176, 770
Pfl23II CGTACG 1 cut(s) 70
PkrI GCNGC 4 cut(s) 9, 107, 590, 715
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Ppu21I YACGTR 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 494
PspFI CCCAGC 2 cut(s) 10, 708
PspGI CCWGG 1 cut(s) 494
PspLI CGTACG 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 500
PspPI GGNCC 2 cut(s) 498, 689
PstI CTGCAG 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 677
RsaI GTAC 3 cut(s) 72, 629, 834
RsaNI GTAC 3 cut(s) 71, 628, 833
SacI GAGCTC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 783, 865
SatI GCNGC 4 cut(s) 8, 106, 589, 714
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 2 cut(s) 498, 689
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 3 cut(s) 338, 496, 502
SduI GDGCHC 2 cut(s) 309, 350
SfaNI GCATC 3 cut(s) 362, 529, 628
SfcI CTRYAG 1 cut(s) 522
SinI GGWCC 1 cut(s) 689
Sse9I AATT 7 cut(s) 207, 408, 441, 450, 571, 748, 761
SsiI CCGC 6 cut(s) 7, 25, 105, 108, 218, 351
SspMI CTAG 1 cut(s) 456
SstI GAGCTC 1 cut(s) 350
StyD4I CCNGG 3 cut(s) 336, 494, 500
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 2 cut(s) 625, 673
TaiI ACGT 2 cut(s) 72, 90
TaqI TCGA 3 cut(s) 241, 356, 759
TasI AATT 7 cut(s) 207, 408, 441, 450, 571, 748, 761
TauI GCSGC 2 cut(s) 10, 108
TfiI GAWTC 2 cut(s) 176, 770
Tru1I TTAA 2 cut(s) 783, 865
Tru9I TTAA 2 cut(s) 783, 865
TseFI GTSAC 2 cut(s) 667, 800
TseI GCWGC 2 cut(s) 588, 713
Tsp45I GTSAC 2 cut(s) 667, 800
TspDTI ATGAA 3 cut(s) 168, 250, 413
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 2 cut(s) 408, 571
XmaJI CCTAGG 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.