MD01G1233500.v1.1

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002111
Physical Location & Seq
Forward (+)
32138278 .. 32141454
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1233500.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGGATGCAGGATCAATGAGTTCTCCATCTAACCTGAAGTCGCAGTCTAGGTGCCCTCTTCAAGAACAGTTTCTTCAGAGAAAGAATTCTCGGGAAAATTTGGACAGGTTCATACCAAACAGGTCAGCAATGGATTTTGATTTCGCAAACATGATGTTAACTGAAGGTAGGAAAGGTAAGGAAAACCCAGCTGTCTCTTCCCCATCCAGAGAGGCCTATCGGAAGCAGTTGGCAGAGGCAATGAACATGAACCGGAGTAGAATTCTTGCATTCAAGAACAAGCCACCTGCCCCTGTTGAGATGTTCCCACGCGAGTTCTCTTCTCTGCAACAGGATAAACCCACAAAGCCCCGAAGGCACATTCCTCAAACTTCAGAGAAGACATTGGATGCTCCAGACCTTGTGGATGATTACTACCTCAATTTGTTGGATTGGGGAAGCTGCAATGTCCTCGCAATAGCTCTTGCAAGCACAGTTTATTTGTGGGATGCTTCCCATGGTTCTACTTCGGAACTGGTCACATTTGAGGATGGAGTAGGATCTGTTACCAGCGTCAGTTGGGCTCCTGATGGGCGCCACATTGCCATTGGACTGGACAGTTCTGAAGTACAGTTATGGGATTCGGTTGCTAAAAAGCAGCTGAGAACTTTGAGAGGTTGCCACAGATCACGAGTAGGCTCTTTGGCATGGAACAACCACATTCTTACAACTGGAGGGATGGACGGTTGCATTGTTAACAATGATGTAAGAATTAGATCACACATTGTTGAGACATACAGAGGACATGAGCAAGAGGTTTGTGGACTGAAGTGGTCAGCCTCGGGCCAGCAATTGGCTAGCGGAGGGAACGACAACCTTCTCCATATATGGGACAGATCAGTGGCATCTTCAAATTCACCAACTCAGTGGCTTCACAGGCTTGAAGATCACACAGCTGCAGTGAAAGCCCTTGCTTGGTGTCCTTTCCAGGGCAATTTATTGGCTTCTGGTGGAGGCGGGGGTGACCGGTGCATAAAGTTTTGGAACACTCATACAGGTGCATGCTTGAACTCGGTCGACACTGGCTCTCAGGTCTGCGCTTTGCTGTGGAACAAGAATGAAAGGGAACTGCTTAGCTCACATGGGTTTACTCAGAATCAGCTCACCCTTTGGAAATACCCTTCGATGGCTAAAATTGCCGAGCTCACAGGCCATACTTCTAGAGTTCTTTATATGGCTCAGAGCCCCGATGGTTGCACGGTCGCATCAGCAGCTGGTGATGAAACACTCAGATTCTGGAACGTTTTTGGGGTCCCCGAGGTTGCTAAACCTGCTCCCAAAGCTAACCCTGAGCCCTTTGCTCATCTGAACCGTATTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

454

Amino Acids

50.18

Weight (kDa)

8.28

Isoelectric Point (pI)

56.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 129 - 426 3.7e-124 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 140 - 232 3.6e-06 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 148 - 244 1.8e-06 WDR19 first beta-propeller
Beta-prop_WDR3_1st PF25173 151 - 249 2.7e-11 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 152 - 291 8.9e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 153 - 293 4.3e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 153 - 230 1.1e-06 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 157 - 292 2.9e-18 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 157 - 290 1.2e-08 WDR36/Utp21 first beta-propeller
Beta-prop_EML_2 PF23414 157 - 298 1.4e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 165 - 269 1.7e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 184 - 302 8.5e-07 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 223 - 421 7.1e-07 Aladin seven-bladed propeller
WD40_Prp19 PF24814 231 - 427 8.1e-21 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 235 - 385 5e-18 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 250 - 428 2.8e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 254 - 372 2e-14 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 255 - 352 5.1e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 256 - 291 1e-07 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 257 - 360 8.7e-07 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 262 - 371 7.6e-20 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 269 - 427 6.2e-06 WDR19 first beta-propeller
Beta-prop_WDR5 PF25175 307 - 428 6.5e-12 WDR5 beta-propeller domain
Beta-prop_EML PF23409 309 - 430 4.1e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 310 - 428 6.2e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 334 - 428 5.3e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 357 - 428 8.1e-09 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 358 - 428 4.3e-06 WDR3 first beta-propeller domain
WD40 PF00400 392 - 427 5e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 2 cut(s) 295, 1318
AccB1I GGYRCC 2 cut(s) 51, 573
AccB7I CCANNNNNTGG 1 cut(s) 832
AccI GTMKAC 1 cut(s) 1056
AccII CGCG 1 cut(s) 312
AciI CCGC 2 cut(s) 840, 996
AclI AACGTT 1 cut(s) 1281
AclWI GGATC 2 cut(s) 19, 547
AcsI RAATTY 4 cut(s) 85, 97, 261, 892
AcuI CTGAAG 6 cut(s) 56, 59, 183, 357, 624, 827
AcyI GRCGYC 1 cut(s) 574
AfaI GTAC 1 cut(s) 609
AfiI CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
AgeI ACCGGT 1 cut(s) 1005
AgsI TTSAA 5 cut(s) 62, 274, 891, 923, 1048
AjnI CCWGG 1 cut(s) 966
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 2 cut(s) 199, 764
AlwI GGATC 2 cut(s) 19, 547
AlwNI CAGNNNCTG 2 cut(s) 1253, 1275
Ama87I CYCGRG 3 cut(s) 90, 820, 1295
AoxI GGCC 3 cut(s) 213, 823, 1189
ApeKI GCWGC 4 cut(s) 441, 637, 935, 1250
ApoI RAATTY 4 cut(s) 85, 97, 261, 892
AsiGI ACCGGT 1 cut(s) 1005
Asp700I GAANNNNTTC 2 cut(s) 69, 85
AspLEI GCGC 2 cut(s) 576, 1079
AspS9I GGNCC 2 cut(s) 823, 1291
AsuHPI GGTGA 4 cut(s) 888, 1013, 1135, 1268
AsuNHI GCTAGC 1 cut(s) 836
AvaI CYCGRG 3 cut(s) 90, 820, 1295
AvaII GGWCC 1 cut(s) 1291
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 2 cut(s) 51, 573
BanII GRGCYC 4 cut(s) 565, 1185, 1226, 1335
BauI CACGAG 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 4 cut(s) 428, 649, 922, 1262
BccI CCATC 7 cut(s) 34, 211, 524, 563, 712, 1159, 1223
BcgI CGANNNNNNTGC 2 cut(s) 433, 467
BciT130I CCWGG 1 cut(s) 968
BcoDI GTCTC 2 cut(s) 199, 764
BfaI CTAG 3 cut(s) 48, 837, 1200
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 1 cut(s) 577
BfuAI ACCTGC 2 cut(s) 295, 1318
BglI GCCNNNNNGGC 1 cut(s) 355
BisI GCNGC 4 cut(s) 442, 638, 936, 1251
BlpI GCTNAGC 1 cut(s) 1112
BlsI GCNGC 4 cut(s) 443, 639, 937, 1252
Bme1390I CCNGG 1 cut(s) 968
Bme18I GGWCC 1 cut(s) 1291
BmeT110I CYCGRG 3 cut(s) 90, 820, 1295
BmgT120I GGNCC 2 cut(s) 823, 1291
BmiI GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
BmrFI CCNGG 1 cut(s) 968
BmsI GCATC 4 cut(s) 379, 478, 893, 1253
BmtI GCTAGC 1 cut(s) 840
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 2 cut(s) 378, 732
Bpu10I CCTNAGC 1 cut(s) 1329
Bpu1102I GCTNAGC 1 cut(s) 1112
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 4 cut(s) 496, 819, 967, 1296
BsaWI WCCGGW 2 cut(s) 252, 1005
Bsc4I CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
Bse118I RCCGGY 1 cut(s) 1005
Bse1I ACTGG 4 cut(s) 519, 597, 715, 1066
Bse3DI GCAATG 4 cut(s) 135, 246, 451, 579
BseBI CCWGG 1 cut(s) 968
BseDI CCNNGG 4 cut(s) 496, 819, 967, 1296
BseGI GGATG 7 cut(s) 10, 203, 394, 412, 493, 535, 723
BseLI CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
BseMI GCAATG 4 cut(s) 135, 246, 451, 579
BseMII CTCAG 7 cut(s) 632, 917, 1082, 1145, 1232, 1282, 1320
BseNI ACTGG 4 cut(s) 519, 597, 715, 1066
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 4 cut(s) 428, 649, 922, 1262
BseYI CCCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 2 cut(s) 1056, 1242
BshFI GGCC 3 cut(s) 215, 825, 1191
BshNI GGYRCC 2 cut(s) 51, 573
BshTI ACCGGT 1 cut(s) 1005
BsiEI CGRYCG 2 cut(s) 1056, 1242
BsiHKAI GWGCWC 1 cut(s) 1185
BsiHKCI CYCGRG 3 cut(s) 90, 820, 1295
BsiSI CCGG 2 cut(s) 253, 1006
BslFI GGGAC 2 cut(s) 884, 1277
BslI CCNNNNNNNGG 5 cut(s) 832, 868, 954, 968, 1165
BsmAI GTCTC 2 cut(s) 199, 764
BsmFI GGGAC 2 cut(s) 884, 1277
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 3 cut(s) 215, 825, 1191
BsoBI CYCGRG 3 cut(s) 90, 820, 1295
Bsp1286I GDGCHC 5 cut(s) 56, 565, 1185, 1226, 1335
Bsp143I GATC 6 cut(s) 11, 539, 665, 755, 875, 925
Bsp1720I GCTNAGC 1 cut(s) 1112
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 2 cut(s) 840, 996
BspANI GGCC 3 cut(s) 215, 825, 1191
BspCNI CTCAG 7 cut(s) 633, 916, 1081, 1144, 1231, 1281, 1321
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
BspMAI CTGCAG 1 cut(s) 940
BspMI ACCTGC 2 cut(s) 295, 1318
BspOI GCTAGC 1 cut(s) 840
BspPI GGATC 2 cut(s) 19, 547
BspT107I GGYRCC 2 cut(s) 51, 573
BsrDI GCAATG 4 cut(s) 135, 246, 451, 579
BsrFI RCCGGY 1 cut(s) 1005
BsrI ACTGG 4 cut(s) 519, 597, 715, 1066
BssAI RCCGGY 1 cut(s) 1005
BssECI CCNNGG 4 cut(s) 496, 819, 967, 1296
BssMI GATC 6 cut(s) 11, 539, 665, 755, 875, 925
BssNI GRCGYC 1 cut(s) 574
BssSI CACGAG 1 cut(s) 669
BssT1I CCWWGG 1 cut(s) 496
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 1 cut(s) 968
Bst4CI ACNGT 7 cut(s) 69, 475, 599, 612, 725, 1240, 1352
Bst6I CTCTTC 3 cut(s) 63, 202, 325
BstACI GRCGYC 1 cut(s) 574
BstC8I GCNNGC 4 cut(s) 469, 827, 838, 1042
BstDEI CTNAG 8 cut(s) 641, 903, 1068, 1112, 1131, 1218, 1268, 1329
BstDSI CCRYGG 1 cut(s) 496
BstEII GGTNACC 1 cut(s) 1001
BstF5I GGATG 7 cut(s) 10, 203, 394, 412, 493, 535, 723
BstFNI CGCG 1 cut(s) 312
BstH2I RGCGCY 1 cut(s) 577
BstHHI GCGC 2 cut(s) 576, 1079
BstKTI GATC 6 cut(s) 14, 542, 668, 758, 878, 928
BstMAI GTCTC 2 cut(s) 199, 764
BstMBI GATC 6 cut(s) 11, 539, 665, 755, 875, 925
BstMCI CGRYCG 2 cut(s) 1056, 1242
BstMWI GCNNNNNNNGC 7 cut(s) 355, 916, 944, 1175, 1250, 1310, 1319
BstNI CCWGG 1 cut(s) 968
BstNSI RCATGY 1 cut(s) 1044
BstPI GGTNACC 1 cut(s) 1001
BstSCI CCNGG 1 cut(s) 966
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 428, 649, 922, 1262
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 2 cut(s) 592, 906
BstYI RGATCY 1 cut(s) 539
BsuRI GGCC 3 cut(s) 215, 825, 1191
BtgI CCRYGG 1 cut(s) 496
BtsCI GGATG 7 cut(s) 10, 203, 394, 412, 493, 535, 723
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 4 cut(s) 885, 911, 945, 1059
BveI ACCTGC 2 cut(s) 295, 1318
Cac8I GCNNGC 4 cut(s) 469, 827, 838, 1042
CaiI CAGNNNCTG 2 cut(s) 1253, 1275
CfoI GCGC 2 cut(s) 576, 1079
Cfr10I RCCGGY 1 cut(s) 1005
Cfr13I GGNCC 2 cut(s) 823, 1291
CseI GACGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 608
CspAI ACCGGT 1 cut(s) 1005
CviAII CATG 7 cut(s) 151, 247, 497, 687, 785, 1041, 1121
CviQI GTAC 1 cut(s) 608
DdeI CTNAG 8 cut(s) 641, 903, 1068, 1112, 1131, 1218, 1268, 1329
DinI GGCGCC 1 cut(s) 575
DpnI GATC 6 cut(s) 13, 541, 667, 757, 877, 927
DpnII GATC 6 cut(s) 11, 539, 665, 755, 875, 925
Eam1104I CTCTTC 3 cut(s) 63, 202, 325
EarI CTCTTC 3 cut(s) 63, 202, 325
Ecl136II GAGCTC 1 cut(s) 1183
Eco130I CCWWGG 1 cut(s) 496
Eco147I AGGCCT 1 cut(s) 215
Eco24I GRGCYC 4 cut(s) 565, 1185, 1226, 1335
Eco47I GGWCC 1 cut(s) 1291
Eco53kI GAGCTC 1 cut(s) 1183
Eco57I CTGAAG 6 cut(s) 56, 59, 183, 357, 624, 827
Eco88I CYCGRG 3 cut(s) 90, 820, 1295
Eco91I GGTNACC 1 cut(s) 1001
EcoICRI GAGCTC 1 cut(s) 1183
EcoO109I RGGNCCY 1 cut(s) 1291
EcoO65I GGTNACC 1 cut(s) 1001
EcoRI GAATTC 2 cut(s) 85, 261
EcoRII CCWGG 1 cut(s) 966
EcoT14I CCWWGG 1 cut(s) 496
EcoT38I GRGCYC 4 cut(s) 565, 1185, 1226, 1335
EgeI GGCGCC 1 cut(s) 575
EheI GGCGCC 1 cut(s) 575
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 7 cut(s) 154, 250, 500, 690, 788, 1044, 1124
FaqI GGGAC 2 cut(s) 884, 1277
FatI CATG 7 cut(s) 150, 246, 496, 686, 784, 1040, 1120
FauI CCCGC 1 cut(s) 989
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 4 cut(s) 442, 638, 936, 1251
FokI GGATG 7 cut(s) 17, 190, 401, 419, 500, 542, 730
FriOI GRGCYC 4 cut(s) 565, 1185, 1226, 1335
Fsp4HI GCNGC 4 cut(s) 442, 638, 936, 1251
FspBI CTAG 3 cut(s) 48, 837, 1200
GlaI GCGC 2 cut(s) 575, 1078
GluI GCNGC 4 cut(s) 442, 638, 936, 1251
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 2 cut(s) 378, 732
HaeII RGCGCY 1 cut(s) 577
HaeIII GGCC 3 cut(s) 215, 825, 1191
HapII CCGG 2 cut(s) 253, 1006
HgaI GACGC 1 cut(s) 541
HhaI GCGC 2 cut(s) 576, 1079
Hin1I GRCGYC 1 cut(s) 574
Hin1II CATG 7 cut(s) 154, 250, 500, 690, 788, 1044, 1124
Hin6I GCGC 2 cut(s) 574, 1077
HinP1I GCGC 2 cut(s) 574, 1077
HincII GTYRAC 3 cut(s) 159, 736, 1057
HindII GTYRAC 3 cut(s) 159, 736, 1057
HinfI GANTC 3 cut(s) 620, 1135, 1272
HpaI GTTAAC 2 cut(s) 159, 736
HpaII CCGG 2 cut(s) 253, 1006
HphI GGTGA 4 cut(s) 888, 1013, 1135, 1268
Hpy166II GTNNAC 5 cut(s) 159, 736, 803, 1057, 1128
Hpy188I TCNGA 9 cut(s) 78, 222, 376, 511, 604, 1134, 1221, 1271, 1347
Hpy188III TCNNGA 9 cut(s) 62, 92, 207, 274, 395, 566, 669, 1200, 1276
Hpy8I GTNNAC 5 cut(s) 159, 736, 803, 1057, 1128
HpyAV CCTTC 4 cut(s) 158, 348, 866, 1170
HpyCH4III ACNGT 7 cut(s) 69, 475, 599, 612, 725, 1240, 1352
HpyCH4IV ACGT 1 cut(s) 1281
HpyF10VI GCNNNNNNNGC 7 cut(s) 355, 916, 944, 1175, 1250, 1310, 1319
HpyF3I CTNAG 8 cut(s) 641, 903, 1068, 1112, 1131, 1218, 1268, 1329
HpySE526I ACGT 1 cut(s) 1281
Hsp92I GRCGYC 1 cut(s) 574
Hsp92II CATG 7 cut(s) 154, 250, 500, 690, 788, 1044, 1124
HspAI GCGC 2 cut(s) 574, 1077
KasI GGCGCC 1 cut(s) 573
KflI GGGWCCC 1 cut(s) 1291
KspAI GTTAAC 2 cut(s) 159, 736
Kzo9I GATC 6 cut(s) 11, 539, 665, 755, 875, 925
LmnI GCTCC 3 cut(s) 397, 568, 1318
Lsp1109I GCAGC 4 cut(s) 428, 649, 922, 1262
LweI GCATC 4 cut(s) 379, 478, 893, 1253
MaeI CTAG 3 cut(s) 48, 837, 1200
MaeII ACGT 1 cut(s) 1281
MaeIII GTNAC 3 cut(s) 517, 544, 1001
MalI GATC 6 cut(s) 13, 541, 667, 757, 877, 927
MboI GATC 6 cut(s) 11, 539, 665, 755, 875, 925
MboII GAAGA 7 cut(s) 50, 65, 189, 312, 391, 879, 935
MfeI CAATTG 1 cut(s) 830
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 5 cut(s) 56, 565, 1185, 1226, 1335
MluCI AATT 9 cut(s) 85, 97, 261, 421, 750, 830, 892, 973, 1173
Mly113I GGCGCC 1 cut(s) 574
MmeI TCCRAC 1 cut(s) 408
MroXI GAANNNNTTC 2 cut(s) 69, 85
MseI TTAA 2 cut(s) 158, 735
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 4 cut(s) 191, 640, 935, 1253
MspI CCGG 2 cut(s) 253, 1006
MspR9I CCNGG 1 cut(s) 968
MunI CAATTG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 1 cut(s) 968
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 7 cut(s) 355, 916, 944, 1175, 1250, 1310, 1319
NarI GGCGCC 1 cut(s) 574
NcoI CCATGG 1 cut(s) 496
NdeII GATC 6 cut(s) 11, 539, 665, 755, 875, 925
NheI GCTAGC 1 cut(s) 836
NlaIII CATG 7 cut(s) 154, 250, 500, 690, 788, 1044, 1124
NlaIV GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
NmeAIII GCCGAG 1 cut(s) 1204
NmuCI GTSAC 2 cut(s) 517, 1001
NspI RCATGY 1 cut(s) 1044
PaeI GCATGC 1 cut(s) 1044
PaqCI CACCTGC 1 cut(s) 295
PceI AGGCCT 1 cut(s) 215
PctI GAATGC 1 cut(s) 269
PdmI GAANNNNTTC 2 cut(s) 69, 85
PfeI GAWTC 3 cut(s) 620, 1135, 1272
PflMI CCANNNNNTGG 1 cut(s) 832
PinAI ACCGGT 1 cut(s) 1005
PkrI GCNGC 4 cut(s) 443, 639, 937, 1252
PluTI GGCGCC 1 cut(s) 577
PpuMI RGGWCCY 1 cut(s) 1291
Psp124BI GAGCTC 1 cut(s) 1185
Psp1406I AACGTT 1 cut(s) 1281
Psp5II RGGWCCY 1 cut(s) 1291
Psp6I CCWGG 1 cut(s) 966
PspEI GGTNACC 1 cut(s) 1001
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 966
PspN4I GGNNCC 5 cut(s) 53, 564, 575, 1292, 1293
PspPI GGNCC 2 cut(s) 823, 1291
PspPPI RGGWCCY 1 cut(s) 1291
PstI CTGCAG 1 cut(s) 940
PstNI CAGNNNCTG 2 cut(s) 1253, 1275
PsuI RGATCY 1 cut(s) 539
PvuII CAGCTG 4 cut(s) 191, 640, 935, 1253
RsaI GTAC 1 cut(s) 609
RsaNI GTAC 1 cut(s) 608
RseI CAYNNNNRTG 1 cut(s) 1035
SacI GAGCTC 1 cut(s) 1185
SalI GTCGAC 1 cut(s) 1055
SaqAI TTAA 2 cut(s) 158, 735
SatI GCNGC 4 cut(s) 442, 638, 936, 1251
Sau3AI GATC 6 cut(s) 11, 539, 665, 755, 875, 925
Sau96I GGNCC 2 cut(s) 823, 1291
ScrFI CCNGG 1 cut(s) 968
SduI GDGCHC 5 cut(s) 56, 565, 1185, 1226, 1335
SfaNI GCATC 4 cut(s) 379, 478, 893, 1253
SfcI CTRYAG 1 cut(s) 936
SfoI GGCGCC 1 cut(s) 575
SinI GGWCC 1 cut(s) 1291
SmiMI CAYNNNNRTG 1 cut(s) 1035
SphI GCATGC 1 cut(s) 1044
Sse9I AATT 9 cut(s) 85, 97, 261, 421, 750, 830, 892, 973, 1173
SseBI AGGCCT 1 cut(s) 215
SsiI CCGC 2 cut(s) 840, 996
SspDI GGCGCC 1 cut(s) 573
SspMI CTAG 3 cut(s) 48, 837, 1200
SstI GAGCTC 1 cut(s) 1185
StuI AGGCCT 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 966
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 7 cut(s) 69, 475, 599, 612, 725, 1240, 1352
TaiI ACGT 1 cut(s) 1284
TaqI TCGA 2 cut(s) 1056, 1163
TaqII GACCGA 1 cut(s) 1042
TasI AATT 9 cut(s) 85, 97, 261, 421, 750, 830, 892, 973, 1173
TatI WGTACW 1 cut(s) 607
TfiI GAWTC 3 cut(s) 620, 1135, 1272
Tru1I TTAA 2 cut(s) 158, 735
Tru9I TTAA 2 cut(s) 158, 735
TscAI CASTG 4 cut(s) 885, 911, 945, 1066
TseFI GTSAC 2 cut(s) 517, 1001
TseI GCWGC 4 cut(s) 441, 637, 935, 1250
Tsp45I GTSAC 2 cut(s) 517, 1001
TspDTI ATGAA 5 cut(s) 100, 257, 263, 1113, 1275
TspRI CASTG 4 cut(s) 885, 911, 945, 1066
Van91I CCANNNNNTGG 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 1291
XapI RAATTY 4 cut(s) 85, 97, 261, 892
XbaI TCTAGA 1 cut(s) 1199
XceI RCATGY 1 cut(s) 1044
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 1056
XmnI GAANNNNTTC 2 cut(s) 69, 85
XspI CTAG 3 cut(s) 48, 837, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.