MD04G1021900.v1.1

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002111
Physical Location & Seq
Reverse (-)
2570015 .. 2572770
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1021900.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGGATTCATCACGAAAGATCAAGTACCGAAGCCCTCTCCAAGAACAGTTCCTTCCGAGAAAGCGCAATCGGGAAAATCTGGACAGGTTTATACCGAATCGAGCGGCAATGGACCTTGATTACGCGAATTACATGGTCACCGGAGGGAGAAAAGGCAAAGAGAACTCGGTTGTAAGCTCTCCATCAAGTGAGGCGTACCGTAAGCTACTGGATGAGACGTTTAACATGAACCGGCCGCGAATCCTGACATTCAAGGAAAAGCCACCAACACCAGTTGAAGCCATTCCGAGGAGGCTACTTTCACCCCCTCCTCATAATGCCAAATCTGCCAAGCCCCGTCGACAGATTCCTCAGGGTTCAGAGAGGACACTGGATGCTCCCGGGATTGTAGATGATTTCTACTTGAATGTACTCGACTGGAGCAGCAATAATGTTCTTGCAATTGCTCTAGGAAACACAGTGTACCTATGGAGTGCTTCTGATGCATCCGTTGAAGAACTTGTCACAGTTGATGATGAAGCTGGCCCCGTTACAAGCATCAGTTGGGCTCCTGATGGGCAGCACGTTGCTGTTGGCTTGAACAATTCCAATGTTCAATTGTGGGATTCCATCTCTGTTAAACTGTTGCGAGTGTTGAGAGATGGTCACCAACAAAGAGTGGGTTCACTGGCGTGGAACAAGCACATACTTACCACAGGCGGTGGGGATAGTAGAATAATCAACAATGACCTTAGAGTGAGATCTCATATAGTTGAAACCTACATCGGTCACCATCAGGAAGTTTGTGGATTGAAATGGTCAGCGTCGGGCCAACAATTAGCTAGTGGAGGGAACGACAATCTTCTCTTTATATGGGATAGGTCGGCAATCTCTTCAACTTCATCACACCAGTGGCTTCACAGGCTTCAAAACCACTCTGCAGCAGTCAAGGCCCTTGCTTGGTGTCCATTCCAAGGGAATTTGCTGGCCTCTGGTGGAGGTGAAGGTGACCAGTGCATTAAGTTCTGGAATGTACACACGGGCTCGTGCTTGAACTCGGTTGAAACTGGTTCACAAGTGTGTGGTCTGGTGTGGAGCAAGAATGAGCGTGAATTGCTTAGTTCTCACGGTTTTAGCCAGAATCACCTGGTCCTTTGGAAATACCCTTCGATGGTGAGGATGGCAGAGCTCAGTGGCCATACATCCAGAATTCTCTTTATGACTCAGAGCCCAGATGGATGTGCTGTGGCCACTGCAGCCGCGGATGAGACTCTTCGGTTTTGGAATGTGTTTGGGATTCCTGAGGTTGCTAAACCCCCACGAGAAGAAAGTTCAATGCCATTTGTTGATTACTGCATACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

448

Amino Acids

49.78

Weight (kDa)

8.4

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 124 - 421 8.9e-122 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 135 - 228 3.3e-09 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 137 - 239 3.4e-07 WDR19 first beta-propeller
Beta-prop_THOC3 PF25174 140 - 290 2e-17 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 142 - 336 3.1e-06 EIPR1 beta-propeller
WD40_Gbeta PF25391 142 - 287 1.7e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 145 - 302 2.6e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 146 - 244 2.8e-11 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 147 - 297 3.6e-11 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 150 - 287 1e-14 WDR5 beta-propeller domain
WD40_Prp19 PF24814 156 - 224 1e-06 Prp19 WD40 domain
ANAPC4_WD40 PF12894 165 - 227 6.5e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_Aladin PF25460 173 - 289 9e-07 Aladin seven-bladed propeller
WD40_Prp19 PF24814 227 - 422 5e-18 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 248 - 360 5.3e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 251 - 380 3.7e-17 WDR3 first beta-propeller domain
WD40 PF00400 251 - 286 1.3e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 251 - 347 1.3e-06 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 253 - 422 2.5e-07 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 257 - 367 6.4e-20 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 259 - 417 2.3e-07 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 264 - 423 1.4e-06 WDR19 first beta-propeller
Beta-prop_WDR5 PF25175 302 - 423 1.8e-11 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 303 - 423 3.3e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 303 - 366 6.9e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 311 - 423 5.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 321 - 423 1.9e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 357 - 423 1.3e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 340
AccII CGCG 3 cut(s) 125, 238, 1241
AciI CCGC 5 cut(s) 104, 236, 699, 1239, 1241
AcoI YGGCCR 3 cut(s) 233, 1174, 1227
AcsI RAATTY 2 cut(s) 958, 1188
AfaI GTAC 5 cut(s) 26, 197, 411, 464, 1014
AfiI CCNNNNNNNGG 4 cut(s) 288, 939, 953, 1150
AjnI CCWGG 1 cut(s) 1125
AleI CACNNNNGTG 3 cut(s) 670, 889, 1057
AluBI AGCT 5 cut(s) 177, 205, 521, 821, 1168
AluI AGCT 5 cut(s) 177, 205, 521, 821, 1168
Alw21I GWGCWC 1 cut(s) 1170
Alw26I GTCTC 2 cut(s) 209, 1241
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 7 cut(s) 233, 523, 808, 930, 966, 1174, 1227
ApeKI GCWGC 4 cut(s) 423, 559, 920, 1235
ApoI RAATTY 2 cut(s) 958, 1188
Asp700I GAANNNNTTC 1 cut(s) 282
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 5 cut(s) 112, 524, 808, 931, 1129
AsuC2I CCSGG 2 cut(s) 381, 382
AsuHPI GGTGA 8 cut(s) 130, 294, 638, 761, 992, 998, 1115, 1165
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 2 cut(s) 112, 1129
AxyI CCTNAGG 2 cut(s) 351, 1281
BalI TGGCCA 2 cut(s) 1176, 1229
BanII GRGCYC 4 cut(s) 550, 1025, 1170, 1211
BauI CACGAG 2 cut(s) 1024, 1299
Bbv12I GWGCWC 1 cut(s) 1170
BbvI GCAGC 4 cut(s) 435, 571, 932, 1247
BccI CCATC 8 cut(s) 190, 548, 617, 635, 780, 1144, 1153, 1208
BciT130I CCWGG 1 cut(s) 1127
BcnI CCSGG 2 cut(s) 381, 382
BcoDI GTCTC 2 cut(s) 209, 1241
BfaI CTAG 2 cut(s) 449, 822
BfmI CTRYAG 2 cut(s) 918, 1233
BglII AGATCT 1 cut(s) 740
BisI GCNGC 7 cut(s) 105, 236, 424, 560, 921, 1236, 1239
BlsI GCNGC 7 cut(s) 106, 237, 425, 561, 922, 1237, 1240
Bme1390I CCNGG 3 cut(s) 381, 382, 1127
Bme18I GGWCC 2 cut(s) 112, 1129
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 5 cut(s) 112, 524, 808, 931, 1129
BmiI GGNNCC 2 cut(s) 526, 549
BmrFI CCNGG 3 cut(s) 381, 382, 1127
BmsI GCATC 4 cut(s) 364, 472, 494, 546
BpmI CTGGAG 1 cut(s) 439
BpuMI CCSGG 2 cut(s) 381, 382
BsaJI CCNNGG 4 cut(s) 287, 380, 952, 1239
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 939, 953, 1150
Bse118I RCCGGY 1 cut(s) 231
Bse1I ACTGG 8 cut(s) 213, 272, 375, 422, 672, 889, 991, 1051
Bse21I CCTNAGG 2 cut(s) 351, 1281
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 1127
BseDI CCNNGG 4 cut(s) 287, 380, 952, 1239
BseGI GGATG 7 cut(s) 217, 379, 485, 1164, 1181, 1223, 1249
BseLI CCNNNNNNNGG 4 cut(s) 288, 939, 953, 1150
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 4 cut(s) 365, 1183, 1217, 1272
BseNI ACTGG 8 cut(s) 213, 272, 375, 422, 672, 889, 991, 1051
BseRI GAGGAG 2 cut(s) 300, 304
BseX3I CGGCCG 1 cut(s) 233
BseXI GCAGC 4 cut(s) 435, 571, 932, 1247
Bsh1236I CGCG 3 cut(s) 125, 238, 1241
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 7 cut(s) 235, 525, 810, 932, 968, 1176, 1229
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 1170
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 3 cut(s) 141, 232, 381
BslI CCNNNNNNNGG 4 cut(s) 288, 939, 953, 1150
BsmAI GTCTC 2 cut(s) 209, 1241
BsmBI CGTCTC 1 cut(s) 209
BsnI GGCC 7 cut(s) 235, 525, 810, 932, 968, 1176, 1229
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 4 cut(s) 550, 1025, 1170, 1211
Bsp1407I TGTACA 1 cut(s) 1012
Bsp143I GATC 2 cut(s) 18, 740
BspACI CCGC 5 cut(s) 104, 236, 699, 1239, 1241
BspANI GGCC 7 cut(s) 235, 525, 810, 932, 968, 1176, 1229
BspCNI CTCAG 4 cut(s) 364, 1182, 1216, 1273
BspFNI CGCG 3 cut(s) 125, 238, 1241
BspLI GGNNCC 2 cut(s) 526, 549
BspMAI CTGCAG 2 cut(s) 922, 1237
BsrBI CCGCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 231
BsrGI TGTACA 1 cut(s) 1012
BsrI ACTGG 8 cut(s) 213, 272, 375, 422, 672, 889, 991, 1051
BssAI RCCGGY 1 cut(s) 231
BssECI CCNNGG 4 cut(s) 287, 380, 952, 1239
BssMI GATC 2 cut(s) 18, 740
BssSI CACGAG 2 cut(s) 1024, 1299
BssT1I CCWWGG 1 cut(s) 952
Bst2BI CACGAG 2 cut(s) 1024, 1299
Bst2UI CCWGG 1 cut(s) 1127
Bst4CI ACNGT 6 cut(s) 48, 200, 460, 508, 624, 1109
Bst6I CTCTTC 2 cut(s) 877, 1257
BstAUI TGTACA 1 cut(s) 1012
BstC8I GCNNGC 2 cut(s) 523, 966
BstDEI CTNAG 6 cut(s) 351, 731, 1097, 1169, 1203, 1281
BstDSI CCRYGG 1 cut(s) 1239
BstEII GGTNACC 4 cut(s) 136, 644, 767, 986
BstF5I GGATG 7 cut(s) 217, 379, 485, 1164, 1181, 1223, 1249
BstFNI CGCG 3 cut(s) 125, 238, 1241
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 2 cut(s) 21, 743
BstMAI GTCTC 2 cut(s) 209, 1241
BstMBI GATC 2 cut(s) 18, 740
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 6 cut(s) 326, 482, 901, 929, 1093, 1235
BstNI CCWGG 1 cut(s) 1127
BstPI GGTNACC 4 cut(s) 136, 644, 767, 986
BstSCI CCNGG 3 cut(s) 379, 380, 1125
BstSFI CTRYAG 2 cut(s) 918, 1233
BstUI CGCG 3 cut(s) 125, 238, 1241
BstV1I GCAGC 4 cut(s) 435, 571, 932, 1247
BstX2I RGATCY 1 cut(s) 740
BstYI RGATCY 1 cut(s) 740
BstZI CGGCCG 1 cut(s) 233
Bsu36I CCTNAGG 2 cut(s) 351, 1281
BsuRI GGCC 7 cut(s) 235, 525, 810, 932, 968, 1176, 1229
BtgI CCRYGG 1 cut(s) 1239
BtsCI GGATG 7 cut(s) 217, 379, 485, 1164, 1181, 1223, 1249
BtsI GCAGTG 1 cut(s) 1230
BtsIMutI CAGTG 7 cut(s) 368, 465, 665, 896, 998, 1177, 1230
Cac8I GCNNGC 2 cut(s) 523, 966
CfoI GCGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 231
Cfr13I GGNCC 5 cut(s) 112, 524, 808, 931, 1129
Cfr42I CCGCGG 1 cut(s) 1242
Cfr9I CCCGGG 1 cut(s) 380
CseI GACGC 1 cut(s) 792
CsiI ACCWGGT 1 cut(s) 1125
Csp6I GTAC 5 cut(s) 25, 196, 410, 463, 1013
CviAII CATG 2 cut(s) 133, 226
CviQI GTAC 5 cut(s) 25, 196, 410, 463, 1013
DdeI CTNAG 6 cut(s) 351, 731, 1097, 1169, 1203, 1281
DpnI GATC 2 cut(s) 20, 742
DpnII GATC 2 cut(s) 18, 740
EaeI YGGCCR 3 cut(s) 233, 1174, 1227
EagI CGGCCG 1 cut(s) 233
Eam1104I CTCTTC 2 cut(s) 877, 1257
EarI CTCTTC 2 cut(s) 877, 1257
Ecl136II GAGCTC 1 cut(s) 1168
EclXI CGGCCG 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 4 cut(s) 550, 1025, 1170, 1211
Eco47I GGWCC 2 cut(s) 112, 1129
Eco52I CGGCCG 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 1168
Eco81I CCTNAGG 2 cut(s) 351, 1281
Eco88I CYCGRG 1 cut(s) 380
Eco91I GGTNACC 4 cut(s) 136, 644, 767, 986
EcoICRI GAGCTC 1 cut(s) 1168
EcoO109I RGGNCCY 1 cut(s) 931
EcoO65I GGTNACC 4 cut(s) 136, 644, 767, 986
EcoRI GAATTC 1 cut(s) 1188
EcoRII CCWGG 1 cut(s) 1125
EcoT14I CCWWGG 1 cut(s) 952
EcoT22I ATGCAT 1 cut(s) 487
EcoT38I GRGCYC 4 cut(s) 550, 1025, 1170, 1211
ErhI CCWWGG 1 cut(s) 952
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 2 cut(s) 136, 229
FatI CATG 2 cut(s) 132, 225
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 7 cut(s) 105, 236, 424, 560, 921, 1236, 1239
FokI GGATG 7 cut(s) 224, 386, 472, 1168, 1171, 1230, 1256
FriOI GRGCYC 4 cut(s) 550, 1025, 1170, 1211
Fsp4HI GCNGC 7 cut(s) 105, 236, 424, 560, 921, 1236, 1239
FspBI CTAG 2 cut(s) 449, 822
GlaI GCGC 1 cut(s) 65
GluI GCNGC 7 cut(s) 105, 236, 424, 560, 921, 1236, 1239
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 7 cut(s) 235, 525, 810, 932, 968, 1176, 1229
HapII CCGG 3 cut(s) 141, 232, 381
HgaI GACGC 1 cut(s) 792
HhaI GCGC 1 cut(s) 66
Hin1II CATG 2 cut(s) 136, 229
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HincII GTYRAC 1 cut(s) 341
HindII GTYRAC 1 cut(s) 341
HinfI GANTC 9 cut(s) 5, 97, 240, 346, 605, 1120, 1201, 1249, 1276
HpaII CCGG 3 cut(s) 141, 232, 381
HphI GGTGA 8 cut(s) 130, 294, 638, 761, 992, 998, 1115, 1165
Hpy166II GTNNAC 5 cut(s) 341, 463, 665, 1015, 1052
Hpy188I TCNGA 5 cut(s) 57, 288, 361, 481, 1206
Hpy188III TCNNGA 9 cut(s) 12, 71, 80, 244, 551, 776, 1006, 1185, 1280
Hpy8I GTNNAC 5 cut(s) 341, 463, 665, 1015, 1052
Hpy99I CGWCG 2 cut(s) 342, 808
HpyAV CCTTC 3 cut(s) 62, 977, 1155
HpyCH4III ACNGT 6 cut(s) 48, 200, 460, 508, 624, 1109
HpyCH4IV ACGT 3 cut(s) 218, 564, 1339
HpyCH4V TGCA 6 cut(s) 440, 485, 920, 996, 1235, 1335
HpyF10VI GCNNNNNNNGC 6 cut(s) 326, 482, 901, 929, 1093, 1235
HpyF3I CTNAG 6 cut(s) 351, 731, 1097, 1169, 1203, 1281
HpySE526I ACGT 3 cut(s) 218, 564, 1339
Hsp92II CATG 2 cut(s) 136, 229
HspAI GCGC 1 cut(s) 64
KspI CCGCGG 1 cut(s) 1242
Kzo9I GATC 2 cut(s) 18, 740
LmnI GCTCC 4 cut(s) 382, 420, 553, 1074
Lsp1109I GCAGC 4 cut(s) 435, 571, 932, 1247
LweI GCATC 4 cut(s) 364, 472, 494, 546
MabI ACCWGGT 1 cut(s) 1125
MaeI CTAG 2 cut(s) 449, 822
MaeII ACGT 3 cut(s) 218, 564, 1339
MaeIII GTNAC 6 cut(s) 136, 502, 529, 644, 767, 986
MalI GATC 2 cut(s) 20, 742
MbiI CCGCTC 1 cut(s) 104
MboI GATC 2 cut(s) 18, 740
MboII GAAGA 5 cut(s) 506, 833, 864, 1244, 1316
MfeI CAATTG 2 cut(s) 441, 596
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 4 cut(s) 550, 1025, 1170, 1211
MlsI TGGCCA 2 cut(s) 1176, 1229
MluCI AATT 8 cut(s) 127, 441, 583, 596, 815, 958, 1091, 1188
MluNI TGGCCA 2 cut(s) 1176, 1229
MlyI GAGTC 2 cut(s) 1195, 1243
Mox20I TGGCCA 2 cut(s) 1176, 1229
Mph1103I ATGCAT 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 282
MscI TGGCCA 2 cut(s) 1176, 1229
MseI TTAA 3 cut(s) 222, 618, 999
MslI CAYNNNNRTG 3 cut(s) 670, 889, 1057
Msp20I TGGCCA 2 cut(s) 1176, 1229
MspA1I CMGCKG 1 cut(s) 1241
MspI CCGG 3 cut(s) 141, 232, 381
MspR9I CCNGG 3 cut(s) 381, 382, 1127
MunI CAATTG 2 cut(s) 441, 596
MvaI CCWGG 1 cut(s) 1127
MvnI CGCG 3 cut(s) 125, 238, 1241
MwoI GCNNNNNNNGC 6 cut(s) 326, 482, 901, 929, 1093, 1235
NciI CCSGG 2 cut(s) 381, 382
NdeII GATC 2 cut(s) 18, 740
NlaIII CATG 2 cut(s) 136, 229
NlaIV GGNNCC 2 cut(s) 526, 549
NmuCI GTSAC 5 cut(s) 136, 502, 644, 767, 986
NsiI ATGCAT 1 cut(s) 487
OliI CACNNNNGTG 3 cut(s) 670, 889, 1057
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 7 cut(s) 5, 97, 240, 346, 605, 1120, 1276
PkrI GCNGC 7 cut(s) 106, 237, 425, 561, 922, 1237, 1240
PleI GAGTC 2 cut(s) 1195, 1243
PpsI GAGTC 2 cut(s) 1195, 1243
Psp124BI GAGCTC 1 cut(s) 1170
Psp6I CCWGG 1 cut(s) 1125
PspEI GGTNACC 4 cut(s) 136, 644, 767, 986
PspGI CCWGG 1 cut(s) 1125
PspN4I GGNNCC 2 cut(s) 526, 549
PspPI GGNCC 5 cut(s) 112, 524, 808, 931, 1129
PstI CTGCAG 2 cut(s) 922, 1237
PsuI RGATCY 1 cut(s) 740
RsaI GTAC 5 cut(s) 26, 197, 411, 464, 1014
RsaNI GTAC 5 cut(s) 25, 196, 410, 463, 1013
RseI CAYNNNNRTG 3 cut(s) 670, 889, 1057
SacI GAGCTC 1 cut(s) 1170
SacII CCGCGG 1 cut(s) 1242
SalI GTCGAC 1 cut(s) 339
SaqAI TTAA 3 cut(s) 222, 618, 999
SatI GCNGC 7 cut(s) 105, 236, 424, 560, 921, 1236, 1239
Sau3AI GATC 2 cut(s) 18, 740
Sau96I GGNCC 5 cut(s) 112, 524, 808, 931, 1129
SchI GAGTC 2 cut(s) 1195, 1243
ScrFI CCNGG 3 cut(s) 381, 382, 1127
SduI GDGCHC 4 cut(s) 550, 1025, 1170, 1211
SexAI ACCWGGT 1 cut(s) 1125
SfaNI GCATC 4 cut(s) 364, 472, 494, 546
SfcI CTRYAG 2 cut(s) 918, 1233
Sfr303I CCGCGG 1 cut(s) 1242
SgrBI CCGCGG 1 cut(s) 1242
SinI GGWCC 2 cut(s) 112, 1129
SmaI CCCGGG 1 cut(s) 382
SmiMI CAYNNNNRTG 3 cut(s) 670, 889, 1057
Sse9I AATT 8 cut(s) 127, 441, 583, 596, 815, 958, 1091, 1188
SsiI CCGC 5 cut(s) 104, 236, 699, 1239, 1241
SspMI CTAG 2 cut(s) 449, 822
SstI GAGCTC 1 cut(s) 1170
StyD4I CCNGG 3 cut(s) 379, 380, 1125
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 6 cut(s) 48, 200, 460, 508, 624, 1109
TaiI ACGT 3 cut(s) 221, 567, 1342
TaqI TCGA 4 cut(s) 100, 340, 414, 1148
TaqII GACCGA 1 cut(s) 755
TasI AATT 8 cut(s) 127, 441, 583, 596, 815, 958, 1091, 1188
TatI WGTACW 2 cut(s) 409, 1012
TauI GCSGC 3 cut(s) 107, 238, 1241
TfiI GAWTC 7 cut(s) 5, 97, 240, 346, 605, 1120, 1276
Tru1I TTAA 3 cut(s) 222, 618, 999
Tru9I TTAA 3 cut(s) 222, 618, 999
TscAI CASTG 7 cut(s) 375, 465, 672, 896, 998, 1177, 1237
TseFI GTSAC 5 cut(s) 136, 502, 644, 767, 986
TseI GCWGC 4 cut(s) 423, 559, 920, 1235
Tsp45I GTSAC 5 cut(s) 136, 502, 644, 767, 986
TspDTI ATGAA 3 cut(s) 242, 531, 870
TspGWI ACGGA 1 cut(s) 478
TspMI CCCGGG 1 cut(s) 380
TspRI CASTG 7 cut(s) 375, 465, 672, 896, 998, 1177, 1237
VpaK11BI GGWCC 2 cut(s) 112, 1129
XapI RAATTY 2 cut(s) 958, 1188
XmaI CCCGGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 2 cut(s) 449, 822
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.