Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002781

Physical Location & Seq

Genomic Coordinates Reverse (-)

13354156 .. 13356884

Gene Structure 2729 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G35080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGAGAATCACGCAGATCTTGTTATGTCTGGTAATAGTGGCATTGTCTTCAAGCTCCCATGTCTGGTCCGATCAGATCTTCCCAGCTCATCTAGTTGGAACATTCAGCAGAAACAATCGTGAACCAAAGTACAAGATTGAGTTCCTTCCTGAAGATTCACCTTTTCACCCGGGTGATAATCTGGAATCAATGGTTATGTTGGATAAGCATGGAAATCGGTTTTTATGTTACTTACCGAAAGAGGAAAAAGCGACGAGTGGATGGACTTCTAGTCAGCAAAATATTAGTACTGTGATGATGGAAACACAGCAACTGGTGAAGCTAAAGACTCCTGATGAATTGCTTCAACCACTTAGTGAAAAATGTCTTTTTAGGCAAGAGGGTTGGTGGTCTTATGAGTTTTGTCATCAGAAGTATGTGCGGCAGCTACACGTTGAGGATGAAAACAAGATTGTTCAAGAGTTTTTTTTGGGTACTTTTGACCCAGAGGCAACTGCGGCGTTTAATCAAACTGTTTCTGATGCTTCAACTGATGCATCTCAGAGGTATCACTCTCATGTATACACCAATGGGACCACCTGTGATCTTACAGGATCACCTCGCGAAGTCGAGGTGAGGTTTGTATGCGCAGAAACAAGGGCAATGGTCACTTCAATCACTGAATTATCAACTTGCAAGTACGCACTGACTGTTCAGTGCCCAACCTTGTGCAAGCATCCGCTGTTCCAGCTAGAGAAACCAGTGTCACACACGATCCACTGCAATGCGATCCCAGTGGAAGAAGACGCAACAAGAAACAAGGAAGAACAAGCAGTAGACGAATCACCTAAGATGATAGCTGATTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0015031 GO:0015833 GO:0019538 GO:0019941 GO:0030163 GO:0030433 GO:0030970 GO:0031974 GO:0032527 GO:0033036 GO:0033554 GO:0034613 GO:0034976 GO:0036503 GO:0042221 GO:0042886 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0070013 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1903513

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

PRKCSH

Protein Analysis

282

Amino Acids

32.17

Weight (kDa)

5.38

Isoelectric Point (pI)

46.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRKCSH_1	PF13015	104 - 251	9e-07	Glucosidase II beta subunit-like protein
PRKCSH	PF07915	121 - 194	1.4e-19	Glucosidase II beta subunit-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002781)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35080
fragaria_vesca	FvH4_1g02000
malus_domestica	MD02G1018200.v1.1 MD15G1162200.v1.1
prunus_persica	Prupe.7G257600_v2.0.a1 Prupe.7G257600_v2.0.a1 Prupe.7G257600_v2.0.a1
pyrus_communis	pycom02g01630
rosa_chinensis	RchiOBHm_Chr1g0314561 RchiOBHm_Chr2g0087061 RchiOBHm_Chr5g0069501 RchiOBHm_Chr7g0241391
rosa_laevigata	RLG00000002275 RLG00000015825 RLG00000036067 RLG00000036074
rosa_multiflora	Rmu_sc0000902.1_g000015 Rmu_sc0010698.1_g000015
rosa_roxburghii	Rroxscaffold_2G00153930
rosa_rugosa	Rorug01G0471800.1 Rorug01G0471900 Rorug02G0634900 Rorug07G0186900
rosa_samantha	Rh1AG011400 Rh2AG025300 Rh2BG025100 Rh2BG052100 Rh2BG213900 Rh2CG025600 Rh2CG054200 Rh2DG025500 Rh5CG497900 Rh7BG320700 Rh7CG348300 Rh7CG436400
rosa_wichuraiana	Rw0G022880 Rw2G002010 Rw4G004450 Rw7G027860

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	628
AccI	GTMKAC	2 cut(s)	561, 816
AccII	CGCG	1 cut(s)	603
AciI	CCGC	3 cut(s)	421, 497, 719
AclWI	GGATC	3 cut(s)	601, 748, 763
AcuI	CTGAAG	1 cut(s)	171
AdeI	CACNNNGTG	1 cut(s)	356
AfaI	GTAC	4 cut(s)	131, 289, 475, 680
AfiI	CCNNNNNNNGG	1 cut(s)	63
AflIII	ACRYGT	1 cut(s)	430
AgsI	TTSAA	5 cut(s)	51, 347, 458, 528, 654
AhdI	GACNNNNNGTC	1 cut(s)	270
AleI	CACNNNNGTG	1 cut(s)	171
AluBI	AGCT	6 cut(s)	54, 86, 322, 427, 730, 839
AluI	AGCT	6 cut(s)	54, 86, 322, 427, 730, 839
AlwI	GGATC	3 cut(s)	601, 748, 763
AlwNI	CAGNNNCTG	1 cut(s)	313
Ama87I	CYCGRG	1 cut(s)	169
ApeKI	GCWGC	1 cut(s)	424
Asp700I	GAANNNNTTC	1 cut(s)	342
AspLEI	GCGC	1 cut(s)	629
AspS9I	GGNCC	2 cut(s)	66, 573
AsuC2I	CCSGG	2 cut(s)	170, 171
AsuHPI	GGTGA	7 cut(s)	150, 158, 185, 328, 588, 625, 816
AvaI	CYCGRG	1 cut(s)	169
AvaII	GGWCC	2 cut(s)	66, 573
BaeGI	GKGCMC	1 cut(s)	701
BbsI	GAAGAC	2 cut(s)	39, 789
BbvI	GCAGC	1 cut(s)	436
BccI	CCATC	2 cut(s)	255, 292
BcgI	CGANNNNNNTGC	2 cut(s)	197, 231
BcnI	CCSGG	2 cut(s)	170, 171
BfaI	CTAG	3 cut(s)	92, 270, 731
BglII	AGATCT	2 cut(s)	15, 75
BisI	GCNGC	3 cut(s)	422, 425, 498
BlsI	GCNGC	3 cut(s)	423, 426, 499
BmcAI	AGTACT	1 cut(s)	289
Bme1390I	CCNGG	2 cut(s)	170, 171
Bme18I	GGWCC	2 cut(s)	66, 573
BmeRI	GACNNNNNGTC	1 cut(s)	270
BmeT110I	CYCGRG	1 cut(s)	169
BmgT120I	GGNCC	2 cut(s)	66, 573
BmiI	GGNNCC	1 cut(s)	574
BmrFI	CCNGG	2 cut(s)	170, 171
BmrI	ACTGGG	1 cut(s)	767
BmsI	GCATC	4 cut(s)	511, 523, 545, 724
BmuI	ACTGGG	1 cut(s)	767
BpiI	GAAGAC	2 cut(s)	39, 789
BpuMI	CCSGG	2 cut(s)	170, 171
BsaJI	CCNNGG	1 cut(s)	169
Bsc4I	CCNNNNNNNGG	1 cut(s)	63
Bse1I	ACTGG	3 cut(s)	318, 740, 773
Bse3DI	GCAATG	2 cut(s)	648, 769
BseDI	CCNNGG	1 cut(s)	169
BseGI	GGATG	3 cut(s)	266, 445, 715
BseLI	CCNNNNNNNGG	1 cut(s)	63
BseMI	GCAATG	2 cut(s)	648, 769
BseMII	CTCAG	1 cut(s)	554
BseNI	ACTGG	3 cut(s)	318, 740, 773
BseSI	GKGCMC	1 cut(s)	701
BseXI	GCAGC	1 cut(s)	436
BseYI	CCCAGC	1 cut(s)	82
Bsh1236I	CGCG	1 cut(s)	603
BsiHKCI	CYCGRG	1 cut(s)	169
BsiSI	CCGG	1 cut(s)	170
BslFI	GGGAC	1 cut(s)	586
BslI	CCNNNNNNNGG	1 cut(s)	63
BsmFI	GGGAC	1 cut(s)	586
BsoBI	CYCGRG	1 cut(s)	169
Bsp1286I	GDGCHC	1 cut(s)	701
Bsp143I	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
Bsp68I	TCGCGA	1 cut(s)	603
BspACI	CCGC	3 cut(s)	421, 497, 719
BspCNI	CTCAG	1 cut(s)	553
BspFNI	CGCG	1 cut(s)	603
BspLI	GGNNCC	1 cut(s)	574
BspPI	GGATC	3 cut(s)	601, 748, 763
BsrDI	GCAATG	2 cut(s)	648, 769
BsrI	ACTGG	3 cut(s)	318, 740, 773
BssECI	CCNNGG	1 cut(s)	169
BssMI	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
BssNAI	GTATAC	1 cut(s)	562
Bst1107I	GTATAC	1 cut(s)	562
Bst4CI	ACNGT	3 cut(s)	292, 514, 691
BstC8I	GCNNGC	1 cut(s)	713
BstDEI	CTNAG	3 cut(s)	353, 540, 828
BstF5I	GGATG	3 cut(s)	266, 445, 715
BstFNI	CGCG	1 cut(s)	603
BstHHI	GCGC	1 cut(s)	629
BstKTI	GATC	7 cut(s)	18, 73, 78, 586, 596, 756, 771
BstMBI	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
BstMWI	GCNNNNNNNGC	2 cut(s)	497, 727
BstSCI	CCNGG	2 cut(s)	168, 169
BstSLI	GKGCMC	1 cut(s)	701
BstUI	CGCG	1 cut(s)	603
BstV1I	GCAGC	1 cut(s)	436
BstV2I	GAAGAC	2 cut(s)	39, 789
BstX2I	RGATCY	2 cut(s)	15, 75
BstYI	RGATCY	2 cut(s)	15, 75
BstZ17I	GTATAC	1 cut(s)	562
BtsCI	GGATG	3 cut(s)	266, 445, 715
BtsI	GCAGTG	1 cut(s)	757
BtsIMutI	CAGTG	6 cut(s)	657, 683, 701, 747, 757, 780
BtuMI	TCGCGA	1 cut(s)	603
Cac8I	GCNNGC	1 cut(s)	713
CaiI	CAGNNNCTG	1 cut(s)	313
CfoI	GCGC	1 cut(s)	629
Cfr13I	GGNCC	2 cut(s)	66, 573
Cfr9I	CCCGGG	1 cut(s)	169
CseI	GACGC	1 cut(s)	794
Csp6I	GTAC	4 cut(s)	130, 288, 474, 679
CviAII	CATG	3 cut(s)	59, 209, 557
CviJI	RGCY	6 cut(s)	54, 86, 322, 427, 730, 839
CviKI_1	RGCY	6 cut(s)	54, 86, 322, 427, 730, 839
CviQI	GTAC	4 cut(s)	130, 288, 474, 679
DdeI	CTNAG	3 cut(s)	353, 540, 828
DpnI	GATC	7 cut(s)	17, 72, 77, 585, 595, 755, 770
DpnII	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
DraIII	CACNNNGTG	1 cut(s)	356
DriI	GACNNNNNGTC	1 cut(s)	270
Eam1105I	GACNNNNNGTC	1 cut(s)	270
Eco47I	GGWCC	2 cut(s)	66, 573
Eco57I	CTGAAG	1 cut(s)	171
Eco88I	CYCGRG	1 cut(s)	169
EcoT22I	ATGCAT	1 cut(s)	538
FaeI	CATG	3 cut(s)	62, 212, 560
FaqI	GGGAC	1 cut(s)	586
FatI	CATG	3 cut(s)	58, 208, 556
FblI	GTMKAC	2 cut(s)	561, 816
Fnu4HI	GCNGC	3 cut(s)	422, 425, 498
FokI	GGATG	3 cut(s)	273, 452, 702
Fsp4HI	GCNGC	3 cut(s)	422, 425, 498
FspBI	CTAG	3 cut(s)	92, 270, 731
FspI	TGCGCA	1 cut(s)	628
GlaI	GCGC	1 cut(s)	628
GluI	GCNGC	3 cut(s)	422, 425, 498
GsaI	CCCAGC	1 cut(s)	86
HapII	CCGG	1 cut(s)	170
HgaI	GACGC	1 cut(s)	794
HhaI	GCGC	1 cut(s)	629
Hin1II	CATG	3 cut(s)	62, 212, 560
Hin6I	GCGC	1 cut(s)	627
HinP1I	GCGC	1 cut(s)	627
HinfI	GANTC	6 cut(s)	6, 155, 185, 328, 821, 842
HpaII	CCGG	1 cut(s)	170
HphI	GGTGA	7 cut(s)	150, 158, 185, 328, 588, 625, 816
Hpy166II	GTNNAC	3 cut(s)	122, 562, 817
Hpy188I	TCNGA	5 cut(s)	70, 75, 411, 520, 543
Hpy188III	TCNNGA	7 cut(s)	119, 149, 182, 332, 458, 602, 846
Hpy8I	GTNNAC	3 cut(s)	122, 562, 817
Hpy99I	CGWCG	1 cut(s)	256
HpyAV	CCTTC	1 cut(s)	155
HpyCH4III	ACNGT	3 cut(s)	292, 514, 691
HpyCH4IV	ACGT	1 cut(s)	432
HpyCH4V	TGCA	4 cut(s)	536, 675, 711, 762
HpyF10VI	GCNNNNNNNGC	2 cut(s)	497, 727
HpyF3I	CTNAG	3 cut(s)	353, 540, 828
HpySE526I	ACGT	1 cut(s)	432
Hsp92II	CATG	3 cut(s)	62, 212, 560
HspAI	GCGC	1 cut(s)	627
Kzo9I	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
LmnI	GCTCC	1 cut(s)	59
Lsp1109I	GCAGC	1 cut(s)	436
LweI	GCATC	4 cut(s)	511, 523, 545, 724
MaeI	CTAG	3 cut(s)	92, 270, 731
MaeII	ACGT	1 cut(s)	432
MaeIII	GTNAC	3 cut(s)	227, 646, 744
MalI	GATC	7 cut(s)	17, 72, 77, 585, 595, 755, 770
MboI	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
MboII	GAAGA	6 cut(s)	39, 70, 164, 791, 794, 815
MflI	RGATCY	2 cut(s)	15, 75
MhlI	GDGCHC	1 cut(s)	701
MluCI	AATT	2 cut(s)	338, 662
MlyI	GAGTC	1 cut(s)	322
MmeI	TCCRAC	2 cut(s)	76, 180
MnlI	CCTC	8 cut(s)	235, 373, 430, 481, 537, 604, 609, 609
Mph1103I	ATGCAT	1 cut(s)	538
MroXI	GAANNNNTTC	1 cut(s)	342
MseI	TTAA	1 cut(s)	504
MslI	CAYNNNNRTG	3 cut(s)	171, 555, 762
MspA1I	CMGCKG	1 cut(s)	721
MspI	CCGG	1 cut(s)	170
MspR9I	CCNGG	2 cut(s)	170, 171
MvnI	CGCG	1 cut(s)	603
MwoI	GCNNNNNNNGC	2 cut(s)	497, 727
NciI	CCSGG	2 cut(s)	170, 171
NdeII	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
NlaIII	CATG	3 cut(s)	62, 212, 560
NlaIV	GGNNCC	1 cut(s)	574
NmuCI	GTSAC	2 cut(s)	646, 744
NruI	TCGCGA	1 cut(s)	603
NsbI	TGCGCA	1 cut(s)	628
NsiI	ATGCAT	1 cut(s)	538
OliI	CACNNNNGTG	1 cut(s)	171
PdmI	GAANNNNTTC	1 cut(s)	342
PfeI	GAWTC	5 cut(s)	6, 155, 185, 821, 842
PkrI	GCNGC	3 cut(s)	423, 426, 499
PleI	GAGTC	1 cut(s)	322
PpsI	GAGTC	1 cut(s)	322
PspFI	CCCAGC	1 cut(s)	82
PspN4I	GGNNCC	1 cut(s)	574
PspPI	GGNCC	2 cut(s)	66, 573
PstNI	CAGNNNCTG	1 cut(s)	313
PsuI	RGATCY	2 cut(s)	15, 75
RruI	TCGCGA	1 cut(s)	603
RsaI	GTAC	4 cut(s)	131, 289, 475, 680
RsaNI	GTAC	4 cut(s)	130, 288, 474, 679
RseI	CAYNNNNRTG	3 cut(s)	171, 555, 762
SaqAI	TTAA	1 cut(s)	504
SatI	GCNGC	3 cut(s)	422, 425, 498
Sau3AI	GATC	7 cut(s)	15, 70, 75, 583, 593, 753, 768
Sau96I	GGNCC	2 cut(s)	66, 573
ScaI	AGTACT	1 cut(s)	289
SchI	GAGTC	1 cut(s)	322
ScrFI	CCNGG	2 cut(s)	170, 171
SduI	GDGCHC	1 cut(s)	701
SfaNI	GCATC	4 cut(s)	511, 523, 545, 724
SinI	GGWCC	2 cut(s)	66, 573
SmaI	CCCGGG	1 cut(s)	171
SmiMI	CAYNNNNRTG	3 cut(s)	171, 555, 762
Sse9I	AATT	2 cut(s)	338, 662
SsiI	CCGC	3 cut(s)	421, 497, 719
SspI	AATATT	1 cut(s)	283
SspMI	CTAG	3 cut(s)	92, 270, 731
StyD4I	CCNGG	2 cut(s)	168, 169
TaaI	ACNGT	3 cut(s)	292, 514, 691
TaiI	ACGT	1 cut(s)	435
TaqI	TCGA	1 cut(s)	609
TasI	AATT	2 cut(s)	338, 662
TatI	WGTACW	2 cut(s)	129, 287
TauI	GCSGC	2 cut(s)	424, 500
TfiI	GAWTC	5 cut(s)	6, 155, 185, 821, 842
Tru1I	TTAA	1 cut(s)	504
Tru9I	TTAA	1 cut(s)	504
TscAI	CASTG	6 cut(s)	664, 690, 701, 747, 764, 780
TseFI	GTSAC	2 cut(s)	646, 744
TseI	GCWGC	1 cut(s)	424
Tsp45I	GTSAC	2 cut(s)	646, 744
TspDTI	ATGAA	2 cut(s)	351, 456
TspMI	CCCGGG	1 cut(s)	169
TspRI	CASTG	6 cut(s)	664, 690, 701, 747, 764, 780
VpaK11BI	GGWCC	2 cut(s)	66, 573
XmaI	CCCGGG	1 cut(s)	169
XmiI	GTMKAC	2 cut(s)	561, 816
XmnI	GAANNNNTTC	1 cut(s)	342
XspI	CTAG	3 cut(s)	92, 270, 731
ZrmI	AGTACT	1 cut(s)	289
Zsp2I	ATGCAT	1 cut(s)	538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.