Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002781

Physical Location & Seq

Genomic Coordinates Reverse (-)

1345348 .. 1348392

Gene Structure 3045 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1018200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 873 bp

ATGAAGGTGTTGGTGTTTTTGGTTCAAGTTTTGTACGTTCTGAGCTGCCTCGTCTCCGCCGATCAGATCTTCCCAGCTCACCTAGGTGGCACATTTCGTCGCAGCTCTCGTGAGCCAAAATACAGGATTGAATTCCATCCTGAAGACTCACCTTTTCATCCTGATGATGGCCAGGAGTCTCTGGTTATGAATAACAATGGACAAAATTTTATATGCTACTTGCCTAAGGTGGAGGAGGCCAAGAGTGGGAAGCAAGTTCTTCAGCAGAATATTAGCAGCATGATTGTGGAAACTGAGAAACGAATTAAAATGAAGACACCGGATGAACTGCTTGAAGTACTCGAGGACCAATGCTTTGTCAGACAAGAGGGTTGGTGGTCATATGAATTTTGCTATCACAAGAGATTACGACAAATTCATGTGGAAGATGATAAGGTGGTTCAGGAATTTGTTTTGGGTGTATATGATGCAGAGGCCACTGCTGGTGTCAACCGGAACCTCTCTGACATATCTACACTCAAAGATCCACGCTCAAAAGACGCATACCAAAGGTACCATGCTCATCAATATACAAACGGGACTACTTGTGATCTTACAGACCTGCAACGAGAGACTGAGGTGAGATTTGTGTGCTCAGAGGGCAGAGCTATGATTAATTCAATCACAGAGGTATCCACATGCAAGTATGCAGTTACAATCCAATGCCCCGCACTTTGCAAGCACCCAGCATTCCAATCAGAGAGACCAGTTTGGCAGACCGTTGATTGTAACGTGCTCCCCAAGGATTACAAGGAAACCAAAGCAGAGGAAGAATCTAATCCGGTTGCAGACGCAGATAGTGAAGATGCCGCTAATGAGGTTTCGGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0015031 GO:0015833 GO:0019538 GO:0019941 GO:0030163 GO:0030433 GO:0030970 GO:0031974 GO:0032527 GO:0033036 GO:0033554 GO:0034613 GO:0034976 GO:0036503 GO:0042221 GO:0042886 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0070013 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1903513

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

PRKCSH

Protein Analysis

291

Amino Acids

33.24

Weight (kDa)

4.99

Isoelectric Point (pI)

51.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRKCSH_1	PF13015	77 - 243	5.2e-06	Glucosidase II beta subunit-like protein
PRKCSH	PF07915	116 - 196	4.9e-18	Glucosidase II beta subunit-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002781)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35080
fragaria_vesca	FvH4_1g02000
malus_domestica	MD02G1018200.v1.1 MD15G1162200.v1.1
prunus_persica	Prupe.7G257600_v2.0.a1 Prupe.7G257600_v2.0.a1 Prupe.7G257600_v2.0.a1
pyrus_communis	pycom02g01630
rosa_chinensis	RchiOBHm_Chr1g0314561 RchiOBHm_Chr2g0087061 RchiOBHm_Chr5g0069501 RchiOBHm_Chr7g0241391
rosa_laevigata	RLG00000002275 RLG00000015825 RLG00000036067 RLG00000036074
rosa_multiflora	Rmu_sc0000902.1_g000015 Rmu_sc0010698.1_g000015
rosa_roxburghii	Rroxscaffold_2G00153930
rosa_rugosa	Rorug01G0471800.1 Rorug01G0471900 Rorug02G0634900 Rorug07G0186900
rosa_samantha	Rh1AG011400 Rh2AG025300 Rh2BG025100 Rh2BG052100 Rh2BG213900 Rh2CG025600 Rh2CG054200 Rh2DG025500 Rh5CG497900 Rh7BG320700 Rh7CG348300 Rh7CG436400
rosa_wichuraiana	Rw0G022880 Rw2G002010 Rw4G004450 Rw7G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	609
Acc65I	GGTACC	1 cut(s)	552
AccB1I	GGYRCC	1 cut(s)	552
AciI	CCGC	3 cut(s)	57, 708, 849
AclWI	GGATC	1 cut(s)	518
AcoI	YGGCCR	1 cut(s)	169
AcsI	RAATTY	5 cut(s)	131, 205, 386, 414, 446
AcuI	CTGAAG	2 cut(s)	162, 245
AfaI	GTAC	3 cut(s)	35, 339, 554
AfiI	CCNNNNNNNGG	2 cut(s)	167, 246
AgsI	TTSAA	4 cut(s)	26, 131, 335, 660
AjnI	CCWGG	1 cut(s)	171
AleI	CACNNNNGTG	1 cut(s)	84
AluBI	AGCT	4 cut(s)	45, 77, 105, 647
AluI	AGCT	4 cut(s)	45, 77, 105, 647
Alw21I	GWGCWC	2 cut(s)	635, 777
Alw26I	GTCTC	4 cut(s)	58, 183, 605, 736
AlwI	GGATC	1 cut(s)	518
Ama87I	CYCGRG	1 cut(s)	341
AoxI	GGCC	3 cut(s)	169, 237, 474
ApeKI	GCWGC	3 cut(s)	45, 102, 276
ApoI	RAATTY	5 cut(s)	131, 205, 386, 414, 446
ArsI	GACNNNNNNTTYG	2 cut(s)	338, 370
AseI	ATTAAT	1 cut(s)	654
Asp718I	GGTACC	1 cut(s)	552
AspA2I	CCTAGG	1 cut(s)	82
AspS9I	GGNCC	1 cut(s)	346
AsuHPI	GGTGA	3 cut(s)	71, 141, 631
AvaI	CYCGRG	1 cut(s)	341
AvaII	GGWCC	1 cut(s)	346
AvrII	CCTAGG	1 cut(s)	82
AxyI	CCTNAGG	1 cut(s)	225
BaeI	ACNNNNGTAYC	2 cut(s)	654, 687
BalI	TGGCCA	1 cut(s)	171
BanI	GGYRCC	1 cut(s)	552
BauI	CACGAG	1 cut(s)	108
BbsI	GAAGAC	2 cut(s)	150, 320
Bbv12I	GWGCWC	2 cut(s)	635, 777
BbvI	GCAGC	3 cut(s)	32, 114, 288
BccI	CCATC	2 cut(s)	144, 161
BciT130I	CCWGG	1 cut(s)	173
BciVI	GTATCC	1 cut(s)	682
BcoDI	GTCTC	4 cut(s)	58, 183, 605, 736
BfaI	CTAG	1 cut(s)	83
BfuAI	ACCTGC	1 cut(s)	609
BfuI	GTATCC	1 cut(s)	682
BglII	AGATCT	1 cut(s)	66
BisI	GCNGC	4 cut(s)	46, 103, 277, 849
BlnI	CCTAGG	1 cut(s)	82
BlsI	GCNGC	4 cut(s)	47, 104, 278, 850
BmcAI	AGTACT	1 cut(s)	339
Bme1390I	CCNGG	1 cut(s)	173
Bme18I	GGWCC	1 cut(s)	346
BmeT110I	CYCGRG	1 cut(s)	341
BmgT120I	GGNCC	1 cut(s)	346
BmiI	GGNNCC	2 cut(s)	497, 554
BmrFI	CCNGG	1 cut(s)	173
BmsI	GCATC	2 cut(s)	457, 835
BpiI	GAAGAC	2 cut(s)	150, 320
BsaI	GGTCTC	1 cut(s)	736
BsaJI	CCNNGG	2 cut(s)	82, 780
BsaWI	WCCGGW	3 cut(s)	319, 492, 820
Bsc4I	CCNNNNNNNGG	2 cut(s)	167, 246
Bse1I	ACTGG	1 cut(s)	746
Bse21I	CCTNAGG	1 cut(s)	225
BseBI	CCWGG	1 cut(s)	173
BseDI	CCNNGG	2 cut(s)	82, 780
BseGI	GGATG	4 cut(s)	136, 157, 328, 871
BseLI	CCNNNNNNNGG	2 cut(s)	167, 246
BseMII	CTCAG	4 cut(s)	32, 285, 606, 648
BseNI	ACTGG	1 cut(s)	746
BseRI	GAGGAG	1 cut(s)	248
BseXI	GCAGC	3 cut(s)	32, 114, 288
BseYI	CCCAGC	2 cut(s)	73, 724
BshFI	GGCC	3 cut(s)	171, 239, 476
BshNI	GGYRCC	1 cut(s)	552
BsiHKAI	GWGCWC	2 cut(s)	635, 777
BsiHKCI	CYCGRG	1 cut(s)	341
BsiSI	CCGG	3 cut(s)	320, 493, 821
BslFI	GGGAC	1 cut(s)	592
BslI	CCNNNNNNNGG	2 cut(s)	167, 246
BsmAI	GTCTC	4 cut(s)	58, 183, 605, 736
BsmBI	CGTCTC	1 cut(s)	58
BsmFI	GGGAC	1 cut(s)	592
BsmI	GAATGC	1 cut(s)	728
BsnI	GGCC	3 cut(s)	171, 239, 476
Bso31I	GGTCTC	1 cut(s)	736
BsoBI	CYCGRG	1 cut(s)	341
Bsp1286I	GDGCHC	2 cut(s)	635, 777
Bsp143I	GATC	4 cut(s)	61, 66, 523, 589
BspACI	CCGC	3 cut(s)	57, 708, 849
BspANI	GGCC	3 cut(s)	171, 239, 476
BspCNI	CTCAG	4 cut(s)	33, 286, 607, 647
BspLI	GGNNCC	2 cut(s)	497, 554
BspMI	ACCTGC	1 cut(s)	609
BspPI	GGATC	1 cut(s)	518
BspT107I	GGYRCC	1 cut(s)	552
BspTNI	GGTCTC	1 cut(s)	736
BsrI	ACTGG	1 cut(s)	746
BssECI	CCNNGG	2 cut(s)	82, 780
BssMI	GATC	4 cut(s)	61, 66, 523, 589
BssSI	CACGAG	1 cut(s)	108
BssT1I	CCWWGG	2 cut(s)	82, 780
Bst2BI	CACGAG	1 cut(s)	108
Bst2UI	CCWGG	1 cut(s)	173
Bst4CI	ACNGT	1 cut(s)	760
BstC8I	GCNNGC	1 cut(s)	719
BstDEI	CTNAG	5 cut(s)	41, 225, 294, 615, 634
BstF5I	GGATG	4 cut(s)	136, 157, 328, 871
BstKTI	GATC	4 cut(s)	64, 69, 526, 592
BstMAI	GTCTC	4 cut(s)	58, 183, 605, 736
BstMBI	GATC	4 cut(s)	61, 66, 523, 589
BstMWI	GCNNNNNNNGC	1 cut(s)	639
BstNI	CCWGG	1 cut(s)	173
BstNSI	RCATGY	1 cut(s)	681
BstSCI	CCNGG	1 cut(s)	171
BstV1I	GCAGC	3 cut(s)	32, 114, 288
BstV2I	GAAGAC	2 cut(s)	150, 320
BstX2I	RGATCY	2 cut(s)	66, 523
BstYI	RGATCY	2 cut(s)	66, 523
Bsu36I	CCTNAGG	1 cut(s)	225
BsuI	GTATCC	1 cut(s)	682
BsuRI	GGCC	3 cut(s)	171, 239, 476
BtsCI	GGATG	4 cut(s)	136, 157, 328, 871
BtsI	GCAGTG	1 cut(s)	477
BtsIMutI	CAGTG	1 cut(s)	477
BveI	ACCTGC	1 cut(s)	609
Cac8I	GCNNGC	1 cut(s)	719
Cfr13I	GGNCC	1 cut(s)	346
CseI	GACGC	2 cut(s)	548, 839
Csp6I	GTAC	3 cut(s)	34, 338, 553
CviAII	CATG	4 cut(s)	280, 419, 557, 678
CviJI	RGCY	8 cut(s)	45, 77, 105, 115, 171, 239, 476, 647
CviKI_1	RGCY	8 cut(s)	45, 77, 105, 115, 171, 239, 476, 647
CviQI	GTAC	3 cut(s)	34, 338, 553
DdeI	CTNAG	5 cut(s)	41, 225, 294, 615, 634
DpnI	GATC	4 cut(s)	63, 68, 525, 591
DpnII	GATC	4 cut(s)	61, 66, 523, 589
EaeI	YGGCCR	1 cut(s)	169
EciI	GGCGGA	1 cut(s)	46
Eco130I	CCWWGG	2 cut(s)	82, 780
Eco31I	GGTCTC	1 cut(s)	736
Eco47I	GGWCC	1 cut(s)	346
Eco57I	CTGAAG	2 cut(s)	162, 245
Eco81I	CCTNAGG	1 cut(s)	225
Eco88I	CYCGRG	1 cut(s)	341
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	1 cut(s)	171
EcoT14I	CCWWGG	2 cut(s)	82, 780
ErhI	CCWWGG	2 cut(s)	82, 780
Esp3I	CGTCTC	1 cut(s)	58
FaeI	CATG	4 cut(s)	283, 422, 560, 681
FaqI	GGGAC	1 cut(s)	592
FatI	CATG	4 cut(s)	279, 418, 556, 677
FauI	CCCGC	1 cut(s)	715
FauNDI	CATATG	1 cut(s)	382
Fnu4HI	GCNGC	4 cut(s)	46, 103, 277, 849
FokI	GGATG	3 cut(s)	123, 144, 335
Fsp4HI	GCNGC	4 cut(s)	46, 103, 277, 849
FspBI	CTAG	1 cut(s)	83
GluI	GCNGC	4 cut(s)	46, 103, 277, 849
GsaI	CCCAGC	2 cut(s)	77, 728
HaeIII	GGCC	3 cut(s)	171, 239, 476
HapII	CCGG	3 cut(s)	320, 493, 821
HgaI	GACGC	2 cut(s)	548, 839
Hin1II	CATG	4 cut(s)	283, 422, 560, 681
HincII	GTYRAC	1 cut(s)	490
HindII	GTYRAC	1 cut(s)	490
HinfI	GANTC	3 cut(s)	146, 176, 812
HpaII	CCGG	3 cut(s)	320, 493, 821
HphI	GGTGA	3 cut(s)	71, 141, 631
Hpy166II	GTNNAC	1 cut(s)	490
Hpy188I	TCNGA	7 cut(s)	42, 66, 362, 505, 637, 739, 865
Hpy188III	TCNNGA	4 cut(s)	110, 140, 161, 443
Hpy8I	GTNNAC	1 cut(s)	490
Hpy99I	CGWCG	1 cut(s)	102
HpyCH4III	ACNGT	1 cut(s)	760
HpyCH4IV	ACGT	2 cut(s)	36, 771
HpyCH4V	TGCA	6 cut(s)	470, 604, 681, 689, 717, 827
HpyF10VI	GCNNNNNNNGC	1 cut(s)	639
HpyF3I	CTNAG	5 cut(s)	41, 225, 294, 615, 634
HpySE526I	ACGT	2 cut(s)	36, 771
Hsp92II	CATG	4 cut(s)	283, 422, 560, 681
KpnI	GGTACC	1 cut(s)	556
Kzo9I	GATC	4 cut(s)	61, 66, 523, 589
LmnI	GCTCC	1 cut(s)	780
Lsp1109I	GCAGC	3 cut(s)	32, 114, 288
LweI	GCATC	2 cut(s)	457, 835
MaeI	CTAG	1 cut(s)	83
MaeII	ACGT	2 cut(s)	36, 771
MaeIII	GTNAC	2 cut(s)	691, 767
MalI	GATC	4 cut(s)	63, 68, 525, 591
MboI	GATC	4 cut(s)	61, 66, 523, 589
MboII	GAAGA	7 cut(s)	61, 155, 251, 325, 437, 821, 854
MflI	RGATCY	2 cut(s)	66, 523
MhlI	GDGCHC	2 cut(s)	635, 777
MlsI	TGGCCA	1 cut(s)	171
MluCI	AATT	7 cut(s)	131, 205, 303, 386, 414, 446, 655
MluNI	TGGCCA	1 cut(s)	171
MlyI	GAGTC	2 cut(s)	140, 185
Mox20I	TGGCCA	1 cut(s)	171
MscI	TGGCCA	1 cut(s)	171
MseI	TTAA	2 cut(s)	306, 654
MslI	CAYNNNNRTG	3 cut(s)	84, 162, 284
Msp20I	TGGCCA	1 cut(s)	171
MspI	CCGG	3 cut(s)	320, 493, 821
MspR9I	CCNGG	1 cut(s)	173
Mva1269I	GAATGC	1 cut(s)	728
MvaI	CCWGG	1 cut(s)	173
MwoI	GCNNNNNNNGC	1 cut(s)	639
NdeI	CATATG	1 cut(s)	382
NdeII	GATC	4 cut(s)	61, 66, 523, 589
NlaIII	CATG	4 cut(s)	283, 422, 560, 681
NlaIV	GGNNCC	2 cut(s)	497, 554
NspI	RCATGY	1 cut(s)	681
OliI	CACNNNNGTG	1 cut(s)	84
PaeR7I	CTCGAG	1 cut(s)	341
PcsI	WCGNNNNNNNCGW	2 cut(s)	57, 106
PctI	GAATGC	1 cut(s)	728
PfeI	GAWTC	1 cut(s)	812
PkrI	GCNGC	4 cut(s)	47, 104, 278, 850
PleI	GAGTC	2 cut(s)	140, 184
PpsI	GAGTC	2 cut(s)	140, 184
PshBI	ATTAAT	1 cut(s)	654
Psp6I	CCWGG	1 cut(s)	171
PspFI	CCCAGC	2 cut(s)	73, 724
PspGI	CCWGG	1 cut(s)	171
PspN4I	GGNNCC	2 cut(s)	497, 554
PspPI	GGNCC	1 cut(s)	346
PspXI	VCTCGAGB	1 cut(s)	341
PsuI	RGATCY	2 cut(s)	66, 523
RsaI	GTAC	3 cut(s)	35, 339, 554
RsaNI	GTAC	3 cut(s)	34, 338, 553
RseI	CAYNNNNRTG	3 cut(s)	84, 162, 284
SaqAI	TTAA	2 cut(s)	306, 654
SatI	GCNGC	4 cut(s)	46, 103, 277, 849
Sau3AI	GATC	4 cut(s)	61, 66, 523, 589
Sau96I	GGNCC	1 cut(s)	346
ScaI	AGTACT	1 cut(s)	339
SchI	GAGTC	2 cut(s)	140, 185
ScrFI	CCNGG	1 cut(s)	173
SduI	GDGCHC	2 cut(s)	635, 777
SfaNI	GCATC	2 cut(s)	457, 835
Sfr274I	CTCGAG	1 cut(s)	341
SinI	GGWCC	1 cut(s)	346
SlaI	CTCGAG	1 cut(s)	341
SmiMI	CAYNNNNRTG	3 cut(s)	84, 162, 284
SmlI	CTYRAG	1 cut(s)	341
SmoI	CTYRAG	1 cut(s)	341
Sse9I	AATT	7 cut(s)	131, 205, 303, 386, 414, 446, 655
SsiI	CCGC	3 cut(s)	57, 708, 849
SspI	AATATT	1 cut(s)	271
SspMI	CTAG	1 cut(s)	83
StyD4I	CCNGG	1 cut(s)	171
StyI	CCWWGG	2 cut(s)	82, 780
TaaI	ACNGT	1 cut(s)	760
TaiI	ACGT	2 cut(s)	39, 774
TaqI	TCGA	1 cut(s)	342
TasI	AATT	7 cut(s)	131, 205, 303, 386, 414, 446, 655
TatI	WGTACW	1 cut(s)	337
TauI	GCSGC	1 cut(s)	851
TfiI	GAWTC	1 cut(s)	812
Tru1I	TTAA	2 cut(s)	306, 654
Tru9I	TTAA	2 cut(s)	306, 654
TscAI	CASTG	1 cut(s)	484
TseI	GCWGC	3 cut(s)	45, 102, 276
TspDTI	ATGAA	7 cut(s)	17, 146, 203, 326, 339, 399, 407
TspRI	CASTG	1 cut(s)	484
VpaK11BI	GGWCC	1 cut(s)	346
VspI	ATTAAT	1 cut(s)	654
XapI	RAATTY	5 cut(s)	131, 205, 386, 414, 446
XceI	RCATGY	1 cut(s)	681
XhoI	CTCGAG	1 cut(s)	341
XmaJI	CCTAGG	1 cut(s)	82
XspI	CTAG	1 cut(s)	83
ZrmI	AGTACT	1 cut(s)	339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.