Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002781

Physical Location & Seq

Genomic Coordinates Reverse (-)

12221684 .. 12224847

Gene Structure 3164 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1162200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 891 bp

ATGAAGGTGTTGGTGTTTTTGGTTCAAGTTTTGTACGTTCTCAGCCACTTCGTCTCGGCCGATCAGATCTTCCTGGATCACCTAGGTGGCGCATTTCGTCGCAGCTCTCGTGAACCAAAATACAGGATTGAATTCCATCCTGAAGACTCACCTTTTCATCCTGATGATGGTCAGGAGTCTGTGGTTATGCCAAATAACAATGGACAAAATTTTATATGTTACTTGCCTAAGGTAGAGGAAGCCAAGAGTGGGAAGCAAGTTCTCCAGCAGAATATTAGCAGCATGATTGTGGAAACTGAGAAACGAATTAAAATGAAGACACCGGATGAGCTGCTTGAAGTACTGAAGGACCAATGCTTTATCAGACAAGAGGGTTGGTGGTCATATGAATTTTGCTATCAGAAGAGATTACGACAAATTCATGTGGAAGATGAAAAGGTGGTTCAGGAATTTGTTTTGGGTGTATATGATGCAGAGGCCACTGTTGCTGTCAACCAGAACCTCTCTGACATATCTACACTCAAAGATCCACGCTCAAAAGATGCATACCAAAGGTACCATGCTCATCAATATACAAATGGGACTACTTGTGATCTTACAGACCTGCAACGAGAGACTGAAGTGAGATTTGTGTGCGCAGAGGGCAAAGCTATGATTAATTCAATTACAGAGGTATCCACATGCAAGTATGCAGTTACAATCCAATGCCCCAAACTTTGCAAGCACCCAGCATTCCAATCAGAGAGACCAGTATGGCAGACAATTGATTGTAACGTGCTTCCCAAGGATTACAAGGAAACCAGAGTAGAGGAAGAATCTAATACGAAGCAGAAAGTTACGGTTGCAGACACAGATAGTGAAGATCCCTCTAATGAGCTTTCGGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0015031 GO:0015833 GO:0019538 GO:0019941 GO:0030163 GO:0030433 GO:0030970 GO:0031974 GO:0032527 GO:0033036 GO:0033554 GO:0034613 GO:0034976 GO:0036503 GO:0042221 GO:0042886 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0070013 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1903513

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

PRKCSH

Protein Analysis

297

Amino Acids

34.22

Weight (kDa)

5.18

Isoelectric Point (pI)

44.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRKCSH	PF07915	117 - 197	4.9e-18	Glucosidase II beta subunit-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002781)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35080
fragaria_vesca	FvH4_1g02000
malus_domestica	MD02G1018200.v1.1 MD15G1162200.v1.1
prunus_persica	Prupe.7G257600_v2.0.a1 Prupe.7G257600_v2.0.a1 Prupe.7G257600_v2.0.a1
pyrus_communis	pycom02g01630
rosa_chinensis	RchiOBHm_Chr1g0314561 RchiOBHm_Chr2g0087061 RchiOBHm_Chr5g0069501 RchiOBHm_Chr7g0241391
rosa_laevigata	RLG00000002275 RLG00000015825 RLG00000036067 RLG00000036074
rosa_multiflora	Rmu_sc0000902.1_g000015 Rmu_sc0010698.1_g000015
rosa_roxburghii	Rroxscaffold_2G00153930
rosa_rugosa	Rorug01G0471800.1 Rorug01G0471900 Rorug02G0634900 Rorug07G0186900
rosa_samantha	Rh1AG011400 Rh2AG025300 Rh2BG025100 Rh2BG052100 Rh2BG213900 Rh2CG025600 Rh2CG054200 Rh2DG025500 Rh5CG497900 Rh7BG320700 Rh7CG348300 Rh7CG436400
rosa_wichuraiana	Rw0G022880 Rw2G002010 Rw4G004450 Rw7G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	637
Acc36I	ACCTGC	1 cut(s)	612
Acc65I	GGTACC	1 cut(s)	555
AccB1I	GGYRCC	1 cut(s)	555
AclWI	GGATC	3 cut(s)	84, 521, 857
AcoI	YGGCCR	1 cut(s)	57
AcsI	RAATTY	5 cut(s)	131, 208, 389, 417, 449
AcuI	CTGAAG	3 cut(s)	162, 365, 639
AfaI	GTAC	3 cut(s)	35, 342, 557
AfiI	CCNNNNNNNGG	2 cut(s)	167, 249
AgsI	TTSAA	4 cut(s)	26, 131, 338, 663
AjnI	CCWGG	1 cut(s)	72
AleI	CACNNNNGTG	1 cut(s)	84
AluBI	AGCT	4 cut(s)	105, 331, 650, 877
AluI	AGCT	4 cut(s)	105, 331, 650, 877
Alw26I	GTCTC	3 cut(s)	58, 608, 739
AlwI	GGATC	3 cut(s)	84, 521, 857
AoxI	GGCC	2 cut(s)	57, 477
ApeKI	GCWGC	3 cut(s)	102, 279, 331
ApoI	RAATTY	5 cut(s)	131, 208, 389, 417, 449
AseI	ATTAAT	1 cut(s)	657
Asp718I	GGTACC	1 cut(s)	555
AspA2I	CCTAGG	1 cut(s)	82
AspLEI	GCGC	2 cut(s)	92, 638
AspS9I	GGNCC	1 cut(s)	349
AsuHPI	GGTGA	2 cut(s)	71, 141
AvaII	GGWCC	1 cut(s)	349
AvrII	CCTAGG	1 cut(s)	82
AxyI	CCTNAGG	1 cut(s)	228
BaeI	ACNNNNGTAYC	2 cut(s)	657, 690
BanI	GGYRCC	1 cut(s)	555
BauI	CACGAG	1 cut(s)	108
BbsI	GAAGAC	2 cut(s)	150, 323
BbvI	GCAGC	3 cut(s)	114, 291, 318
BccI	CCATC	2 cut(s)	144, 161
BciT130I	CCWGG	1 cut(s)	74
BciVI	GTATCC	1 cut(s)	685
BcoDI	GTCTC	3 cut(s)	58, 608, 739
BfaI	CTAG	1 cut(s)	83
BfuAI	ACCTGC	1 cut(s)	612
BfuI	GTATCC	1 cut(s)	685
BglII	AGATCT	1 cut(s)	66
BisI	GCNGC	3 cut(s)	103, 280, 332
BlnI	CCTAGG	1 cut(s)	82
BlsI	GCNGC	3 cut(s)	104, 281, 333
BmcAI	AGTACT	1 cut(s)	342
Bme1390I	CCNGG	1 cut(s)	74
Bme18I	GGWCC	1 cut(s)	349
BmgT120I	GGNCC	1 cut(s)	349
BmiI	GGNNCC	1 cut(s)	557
BmrFI	CCNGG	1 cut(s)	74
BmsI	GCATC	2 cut(s)	460, 532
BpiI	GAAGAC	2 cut(s)	150, 323
BpmI	CTGGAG	1 cut(s)	248
BsaI	GGTCTC	1 cut(s)	739
BsaJI	CCNNGG	2 cut(s)	82, 783
BsaWI	WCCGGW	1 cut(s)	322
Bsc4I	CCNNNNNNNGG	2 cut(s)	167, 249
Bse1I	ACTGG	1 cut(s)	749
Bse21I	CCTNAGG	1 cut(s)	228
BseBI	CCWGG	1 cut(s)	74
BseDI	CCNNGG	2 cut(s)	82, 783
BseGI	GGATG	4 cut(s)	136, 157, 331, 889
BseLI	CCNNNNNNNGG	2 cut(s)	167, 249
BseMII	CTCAG	2 cut(s)	55, 288
BseNI	ACTGG	1 cut(s)	749
BseX3I	CGGCCG	1 cut(s)	57
BseXI	GCAGC	3 cut(s)	114, 291, 318
BseYI	CCCAGC	1 cut(s)	727
Bsh1285I	CGRYCG	1 cut(s)	60
BshFI	GGCC	2 cut(s)	59, 479
BshNI	GGYRCC	1 cut(s)	555
BsiEI	CGRYCG	1 cut(s)	60
BsiSI	CCGG	1 cut(s)	323
BslFI	GGGAC	1 cut(s)	595
BslI	CCNNNNNNNGG	2 cut(s)	167, 249
BsmAI	GTCTC	3 cut(s)	58, 608, 739
BsmBI	CGTCTC	1 cut(s)	58
BsmFI	GGGAC	1 cut(s)	595
BsmI	GAATGC	1 cut(s)	731
BsnI	GGCC	2 cut(s)	59, 479
Bso31I	GGTCTC	1 cut(s)	739
Bsp143I	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
BspANI	GGCC	2 cut(s)	59, 479
BspCNI	CTCAG	2 cut(s)	54, 289
BspLI	GGNNCC	1 cut(s)	557
BspMI	ACCTGC	1 cut(s)	612
BspPI	GGATC	3 cut(s)	84, 521, 857
BspT107I	GGYRCC	1 cut(s)	555
BspTNI	GGTCTC	1 cut(s)	739
BsrI	ACTGG	1 cut(s)	749
BssECI	CCNNGG	2 cut(s)	82, 783
BssMI	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
BssSI	CACGAG	1 cut(s)	108
BssT1I	CCWWGG	2 cut(s)	82, 783
Bst2BI	CACGAG	1 cut(s)	108
Bst2UI	CCWGG	1 cut(s)	74
Bst4CI	ACNGT	2 cut(s)	484, 841
Bst6I	CTCTTC	1 cut(s)	398
BstC8I	GCNNGC	1 cut(s)	722
BstDEI	CTNAG	3 cut(s)	41, 228, 297
BstF5I	GGATG	4 cut(s)	136, 157, 331, 889
BstHHI	GCGC	2 cut(s)	92, 638
BstKTI	GATC	6 cut(s)	64, 69, 79, 529, 595, 865
BstMAI	GTCTC	3 cut(s)	58, 608, 739
BstMBI	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
BstMCI	CGRYCG	1 cut(s)	60
BstMWI	GCNNNNNNNGC	2 cut(s)	485, 642
BstNI	CCWGG	1 cut(s)	74
BstNSI	RCATGY	1 cut(s)	684
BstSCI	CCNGG	1 cut(s)	72
BstV1I	GCAGC	3 cut(s)	114, 291, 318
BstV2I	GAAGAC	2 cut(s)	150, 323
BstX2I	RGATCY	3 cut(s)	66, 526, 862
BstYI	RGATCY	3 cut(s)	66, 526, 862
BstZI	CGGCCG	1 cut(s)	57
Bsu36I	CCTNAGG	1 cut(s)	228
BsuI	GTATCC	1 cut(s)	685
BsuRI	GGCC	2 cut(s)	59, 479
BtsCI	GGATG	4 cut(s)	136, 157, 331, 889
BtsIMutI	CAGTG	1 cut(s)	480
BveI	ACCTGC	1 cut(s)	612
Cac8I	GCNNGC	1 cut(s)	722
CfoI	GCGC	2 cut(s)	92, 638
Cfr13I	GGNCC	1 cut(s)	349
Csp6I	GTAC	3 cut(s)	34, 341, 556
CviAII	CATG	4 cut(s)	283, 422, 560, 681
CviJI	RGCY	8 cut(s)	45, 59, 105, 242, 331, 479, 650, 877
CviKI_1	RGCY	8 cut(s)	45, 59, 105, 242, 331, 479, 650, 877
CviQI	GTAC	3 cut(s)	34, 341, 556
DdeI	CTNAG	3 cut(s)	41, 228, 297
DpnI	GATC	6 cut(s)	63, 68, 78, 528, 594, 864
DpnII	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
EaeI	YGGCCR	1 cut(s)	57
EagI	CGGCCG	1 cut(s)	57
Eam1104I	CTCTTC	1 cut(s)	398
EarI	CTCTTC	1 cut(s)	398
EclXI	CGGCCG	1 cut(s)	57
Eco130I	CCWWGG	2 cut(s)	82, 783
Eco31I	GGTCTC	1 cut(s)	739
Eco47I	GGWCC	1 cut(s)	349
Eco52I	CGGCCG	1 cut(s)	57
Eco57I	CTGAAG	3 cut(s)	162, 365, 639
Eco81I	CCTNAGG	1 cut(s)	228
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	1 cut(s)	72
EcoT14I	CCWWGG	2 cut(s)	82, 783
EcoT22I	ATGCAT	1 cut(s)	547
ErhI	CCWWGG	2 cut(s)	82, 783
Esp3I	CGTCTC	1 cut(s)	58
FaeI	CATG	4 cut(s)	286, 425, 563, 684
FaqI	GGGAC	1 cut(s)	595
FatI	CATG	4 cut(s)	282, 421, 559, 680
FauNDI	CATATG	1 cut(s)	385
Fnu4HI	GCNGC	3 cut(s)	103, 280, 332
FokI	GGATG	3 cut(s)	123, 144, 338
Fsp4HI	GCNGC	3 cut(s)	103, 280, 332
FspBI	CTAG	1 cut(s)	83
FspI	TGCGCA	1 cut(s)	637
GlaI	GCGC	2 cut(s)	91, 637
GluI	GCNGC	3 cut(s)	103, 280, 332
GsaI	CCCAGC	1 cut(s)	731
GsuI	CTGGAG	1 cut(s)	248
HaeIII	GGCC	2 cut(s)	59, 479
HapII	CCGG	1 cut(s)	323
HhaI	GCGC	2 cut(s)	92, 638
Hin1II	CATG	4 cut(s)	286, 425, 563, 684
Hin6I	GCGC	2 cut(s)	90, 636
HinP1I	GCGC	2 cut(s)	90, 636
HincII	GTYRAC	1 cut(s)	493
HindII	GTYRAC	1 cut(s)	493
HinfI	GANTC	3 cut(s)	146, 176, 815
HpaII	CCGG	1 cut(s)	323
HphI	GGTGA	2 cut(s)	71, 141
Hpy166II	GTNNAC	2 cut(s)	113, 493
Hpy188I	TCNGA	6 cut(s)	66, 365, 402, 508, 742, 883
Hpy188III	TCNNGA	5 cut(s)	110, 140, 161, 173, 446
Hpy8I	GTNNAC	2 cut(s)	113, 493
Hpy99I	CGWCG	1 cut(s)	102
HpyAV	CCTTC	1 cut(s)	340
HpyCH4III	ACNGT	2 cut(s)	484, 841
HpyCH4IV	ACGT	2 cut(s)	36, 774
HpyCH4V	TGCA	7 cut(s)	473, 545, 607, 684, 692, 720, 845
HpyF10VI	GCNNNNNNNGC	2 cut(s)	485, 642
HpyF3I	CTNAG	3 cut(s)	41, 228, 297
HpySE526I	ACGT	2 cut(s)	36, 774
Hsp92II	CATG	4 cut(s)	286, 425, 563, 684
HspAI	GCGC	2 cut(s)	90, 636
KpnI	GGTACC	1 cut(s)	559
Kzo9I	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
Lsp1109I	GCAGC	3 cut(s)	114, 291, 318
LweI	GCATC	2 cut(s)	460, 532
MaeI	CTAG	1 cut(s)	83
MaeII	ACGT	2 cut(s)	36, 774
MaeIII	GTNAC	4 cut(s)	218, 694, 770, 835
MalI	GATC	6 cut(s)	63, 68, 78, 528, 594, 864
MboI	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
MboII	GAAGA	7 cut(s)	61, 155, 328, 415, 440, 824, 872
MfeI	CAATTG	1 cut(s)	762
MflI	RGATCY	3 cut(s)	66, 526, 862
MluCI	AATT	9 cut(s)	131, 208, 306, 389, 417, 449, 658, 663, 762
MlyI	GAGTC	2 cut(s)	140, 185
MnlI	CCTC	8 cut(s)	229, 364, 469, 512, 634, 664, 802, 877
Mph1103I	ATGCAT	1 cut(s)	547
MseI	TTAA	2 cut(s)	309, 657
MslI	CAYNNNNRTG	3 cut(s)	84, 162, 287
MspI	CCGG	1 cut(s)	323
MspR9I	CCNGG	1 cut(s)	74
MunI	CAATTG	1 cut(s)	762
Mva1269I	GAATGC	1 cut(s)	731
MvaI	CCWGG	1 cut(s)	74
MwoI	GCNNNNNNNGC	2 cut(s)	485, 642
NdeI	CATATG	1 cut(s)	385
NdeII	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
NlaIII	CATG	4 cut(s)	286, 425, 563, 684
NlaIV	GGNNCC	1 cut(s)	557
NmeAIII	GCCGAG	1 cut(s)	35
NsbI	TGCGCA	1 cut(s)	637
NsiI	ATGCAT	1 cut(s)	547
NspI	RCATGY	1 cut(s)	684
OliI	CACNNNNGTG	1 cut(s)	84
PcsI	WCGNNNNNNNCGW	2 cut(s)	57, 106
PctI	GAATGC	1 cut(s)	731
PfeI	GAWTC	1 cut(s)	815
PfoI	TCCNGGA	1 cut(s)	72
PkrI	GCNGC	3 cut(s)	104, 281, 333
PleI	GAGTC	2 cut(s)	140, 184
PpsI	GAGTC	2 cut(s)	140, 184
PshBI	ATTAAT	1 cut(s)	657
Psp6I	CCWGG	1 cut(s)	72
PspFI	CCCAGC	1 cut(s)	727
PspGI	CCWGG	1 cut(s)	72
PspN4I	GGNNCC	1 cut(s)	557
PspPI	GGNCC	1 cut(s)	349
PsuI	RGATCY	3 cut(s)	66, 526, 862
RsaI	GTAC	3 cut(s)	35, 342, 557
RsaNI	GTAC	3 cut(s)	34, 341, 556
RseI	CAYNNNNRTG	3 cut(s)	84, 162, 287
SaqAI	TTAA	2 cut(s)	309, 657
SatI	GCNGC	3 cut(s)	103, 280, 332
Sau3AI	GATC	6 cut(s)	61, 66, 76, 526, 592, 862
Sau96I	GGNCC	1 cut(s)	349
ScaI	AGTACT	1 cut(s)	342
SchI	GAGTC	2 cut(s)	140, 185
ScrFI	CCNGG	1 cut(s)	74
SfaNI	GCATC	2 cut(s)	460, 532
SinI	GGWCC	1 cut(s)	349
SmiMI	CAYNNNNRTG	3 cut(s)	84, 162, 287
Sse9I	AATT	9 cut(s)	131, 208, 306, 389, 417, 449, 658, 663, 762
SspI	AATATT	1 cut(s)	274
SspMI	CTAG	1 cut(s)	83
StyD4I	CCNGG	1 cut(s)	72
StyI	CCWWGG	2 cut(s)	82, 783
TaaI	ACNGT	2 cut(s)	484, 841
TaiI	ACGT	2 cut(s)	39, 777
TasI	AATT	9 cut(s)	131, 208, 306, 389, 417, 449, 658, 663, 762
TatI	WGTACW	1 cut(s)	340
TfiI	GAWTC	1 cut(s)	815
Tru1I	TTAA	2 cut(s)	309, 657
Tru9I	TTAA	2 cut(s)	309, 657
TscAI	CASTG	1 cut(s)	487
TseI	GCWGC	3 cut(s)	102, 279, 331
TspDTI	ATGAA	6 cut(s)	17, 146, 329, 402, 410, 447
TspRI	CASTG	1 cut(s)	487
VpaK11BI	GGWCC	1 cut(s)	349
VspI	ATTAAT	1 cut(s)	657
XapI	RAATTY	5 cut(s)	131, 208, 389, 417, 449
XceI	RCATGY	1 cut(s)	684
XmaJI	CCTAGG	1 cut(s)	82
XspI	CTAG	1 cut(s)	83
ZrmI	AGTACT	1 cut(s)	342
Zsp2I	ATGCAT	1 cut(s)	547

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.