Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003582

Physical Location & Seq

Genomic Coordinates Forward (+)

22216934 .. 22219274

Gene Structure 2341 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G54680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 705 bp

ATGGTGTCACCCGAAAACGCTAATTGGATTTGTGACTTGATCGATGCTGATTACGGAAGTTTCACAATCCAAGGTCCTGGTTTCTCTTGGCCTGTTCAGCAACCTATTGGTGTTTCTTCTAACTCCAGTGCTGGAGTTGATGGCTCGGCTGGAAACTCAGAAGCTAGCAAAGAACCTGGATCCAAAAAGAGGGGGAGATGTGAATCATCCTCTGCCACTAGCTCGAAAGCATGTAGAGAGAAGCAGCGACGGGACAGGTTGAATGACAAGTTTATGGAATTGGGTGCAATTTTGGAGCCTGGAAATCCTCCCAAAACAGACAAGGCTGCTATCTTGGTTGATGCTGTCCGCATGGTGACACAGCTACGGGGCGAGGCCCAGAAGCTGAAGGACTCCAATTCAAGTCTTCAGGACAAAATCAAAGAGTTAAAGACTGAGAAAAACGAGCTGCGAGATGAGAAACAGAGGCTGAAGACAGAGAAAGAAAAGCTGGAGCAGCAGCTGAAAGCCATGAATGCTCCTCAACCAAGTTTTTTCCCAGCCCCACCTATGATGCCTACTGCTTTTGCTTCAGCGCAAGGCCAAGCTCCTGGAAACAAGATGGTGCCAATCATCAGTTACCCAGGAGTTGCCATGTGGCAGTTCATGCCTCCTGCTTCAGTCGATACTTCTCAGGATCATGTCCTTCGTCCTCCTGTTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016143 GO:0019219 GO:0019222 GO:0019748 GO:0019757 GO:0019760 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044237 GO:0044281 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901135 GO:1901363 GO:1901564 GO:1901657 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

234

Amino Acids

25.49

Weight (kDa)

8.69

Isoelectric Point (pI)

51.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	75 - 123	5.1e-11	Helix-loop-helix DNA-binding domain
bHLH_IRO3	PF23177	75 - 140	1e-08	Iron-related transcription factor 3 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54680
fragaria_vesca	FvH4_3g02910 FvH4_3g02910 FvH4_3g21720
malus_domestica	MD03G1212600.v1.1 MD05G1336500.v1.1 MD05G1336600.v1.1 MD10G1312000.v1.1 MD10G1312100.v1.1 MD11G1227800.v1.1
prunus_persica	Prupe.4G029000_v2.0.a1 Prupe.4G201500_v2.0.a1
pyrus_communis	pycom03g16130 pycom05g30800 pycom10g26330 pycom11g20040
rosa_chinensis	RchiOBHm_Chr5g0004471 RchiOBHm_Chr5g0037201
rosa_laevigata	RLG00000031231 RLG00000033764
rosa_multiflora	Rmu_sc0000433.1_g000001 Rmu_sc0002453.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043770 Rroxscaffold_1G00071050
rosa_rugosa	Rorug04G0412000 Rorug05G0163600
rosa_samantha	Rh5AG039600 Rh5AG253200 Rh5BG038600 Rh5BG255200 Rh5CG042700 Rh5CG287900 Rh5DG038400
rosa_wichuraiana	Rw5G003780 Rw5G023390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	604
AciI	CCGC	1 cut(s)	349
AclWI	GGATC	3 cut(s)	174, 187, 684
AcuI	CTGAAG	5 cut(s)	392, 407, 491, 555, 642
AfiI	CCNNNNNNNGG	1 cut(s)	189
AgsI	TTSAA	2 cut(s)	262, 402
AjnI	CCWGG	5 cut(s)	76, 175, 298, 589, 622
AluBI	AGCT	8 cut(s)	164, 222, 364, 385, 448, 490, 502, 587
AluI	AGCT	8 cut(s)	164, 222, 364, 385, 448, 490, 502, 587
AlwI	GGATC	3 cut(s)	174, 187, 684
AlwNI	CAGNNNCTG	3 cut(s)	385, 469, 502
AoxI	GGCC	3 cut(s)	89, 375, 580
ApeKI	GCWGC	5 cut(s)	244, 326, 448, 496, 499
AspLEI	GCGC	1 cut(s)	577
AspS9I	GGNCC	2 cut(s)	74, 376
AsuHPI	GGTGA	1 cut(s)	367
AsuNHI	GCTAGC	1 cut(s)	164
AvaII	GGWCC	1 cut(s)	74
BamHI	GGATCC	1 cut(s)	179
BanI	GGYRCC	1 cut(s)	604
BbsI	GAAGAC	2 cut(s)	398, 479
BbvI	GCAGC	5 cut(s)	256, 313, 435, 508, 511
BccI	CCATC	2 cut(s)	134, 595
BciT130I	CCWGG	5 cut(s)	78, 177, 300, 591, 624
BfaI	CTAG	2 cut(s)	165, 219
BisI	GCNGC	5 cut(s)	245, 327, 449, 497, 500
BlsI	GCNGC	5 cut(s)	246, 328, 450, 498, 501
Bme1390I	CCNGG	5 cut(s)	78, 177, 300, 591, 624
Bme18I	GGWCC	1 cut(s)	74
BmgT120I	GGNCC	2 cut(s)	74, 376
BmiI	GGNNCC	3 cut(s)	181, 297, 606
BmrFI	CCNGG	5 cut(s)	78, 177, 300, 591, 624
BmsI	GCATC	3 cut(s)	34, 331, 543
BmtI	GCTAGC	1 cut(s)	168
BpiI	GAAGAC	2 cut(s)	398, 479
BpmI	CTGGAG	3 cut(s)	109, 153, 512
Bsa29I	ATCGAT	1 cut(s)	42
BsaBI	GATNNNNATC	1 cut(s)	202
BsaJI	CCNNGG	2 cut(s)	70, 622
Bsc4I	CCNNNNNNNGG	1 cut(s)	189
Bse1I	ACTGG	1 cut(s)	126
Bse8I	GATNNNNATC	1 cut(s)	202
BseBI	CCWGG	5 cut(s)	78, 177, 300, 591, 624
BseCI	ATCGAT	1 cut(s)	42
BseDI	CCNNGG	2 cut(s)	70, 622
BseGI	GGATG	1 cut(s)	206
BseJI	GATNNNNATC	1 cut(s)	202
BseLI	CCNNNNNNNGG	1 cut(s)	189
BseMII	CTCAG	3 cut(s)	171, 426, 686
BseNI	ACTGG	1 cut(s)	126
BseRI	GAGGAG	1 cut(s)	510
BseXI	GCAGC	5 cut(s)	256, 313, 435, 508, 511
BseYI	CCCAGC	1 cut(s)	538
BshFI	GGCC	3 cut(s)	91, 377, 582
BshNI	GGYRCC	1 cut(s)	604
BshVI	ATCGAT	1 cut(s)	42
BslFI	GGGAC	1 cut(s)	266
BslI	CCNNNNNNNGG	1 cut(s)	189
BsmFI	GGGAC	1 cut(s)	266
BsmI	GAATGC	1 cut(s)	520
BsnI	GGCC	3 cut(s)	91, 377, 582
Bsp143I	GATC	3 cut(s)	39, 179, 676
BspACI	CCGC	1 cut(s)	349
BspANI	GGCC	3 cut(s)	91, 377, 582
BspCNI	CTCAG	3 cut(s)	170, 427, 685
BspDI	ATCGAT	1 cut(s)	42
BspLI	GGNNCC	3 cut(s)	181, 297, 606
BspOI	GCTAGC	1 cut(s)	168
BspPI	GGATC	3 cut(s)	174, 187, 684
BspT107I	GGYRCC	1 cut(s)	604
BsrI	ACTGG	1 cut(s)	126
BssECI	CCNNGG	2 cut(s)	70, 622
BssMI	GATC	3 cut(s)	39, 179, 676
BssT1I	CCWWGG	1 cut(s)	70
Bst2UI	CCWGG	5 cut(s)	78, 177, 300, 591, 624
BstAPI	GCANNNNNTGC	1 cut(s)	646
BstC8I	GCNNGC	1 cut(s)	166
BstDEI	CTNAG	3 cut(s)	157, 435, 672
BstF5I	GGATG	1 cut(s)	206
BstHHI	GCGC	1 cut(s)	577
BstKTI	GATC	3 cut(s)	42, 182, 679
BstMBI	GATC	3 cut(s)	39, 179, 676
BstMWI	GCNNNNNNNGC	4 cut(s)	97, 496, 515, 646
BstNI	CCWGG	5 cut(s)	78, 177, 300, 591, 624
BstNSI	RCATGY	1 cut(s)	234
BstSCI	CCNGG	5 cut(s)	76, 175, 298, 589, 622
BstV1I	GCAGC	5 cut(s)	256, 313, 435, 508, 511
BstV2I	GAAGAC	2 cut(s)	398, 479
BstX2I	RGATCY	1 cut(s)	179
BstXI	CCANNNNNNTGG	2 cut(s)	77, 590
BstYI	RGATCY	1 cut(s)	179
Bsu15I	ATCGAT	1 cut(s)	42
BsuRI	GGCC	3 cut(s)	91, 377, 582
BsuTUI	ATCGAT	1 cut(s)	42
BtsCI	GGATG	1 cut(s)	206
BtsIMutI	CAGTG	1 cut(s)	133
Cac8I	GCNNGC	1 cut(s)	166
CaiI	CAGNNNCTG	3 cut(s)	385, 469, 502
CfoI	GCGC	1 cut(s)	577
Cfr13I	GGNCC	2 cut(s)	74, 376
ClaI	ATCGAT	1 cut(s)	42
CviAII	CATG	6 cut(s)	231, 352, 511, 634, 646, 680
DdeI	CTNAG	3 cut(s)	157, 435, 672
DpnI	GATC	3 cut(s)	41, 181, 678
DpnII	GATC	3 cut(s)	39, 179, 676
Eco130I	CCWWGG	1 cut(s)	70
Eco47I	GGWCC	1 cut(s)	74
Eco57I	CTGAAG	5 cut(s)	392, 407, 491, 555, 642
EcoO109I	RGGNCCY	1 cut(s)	74
EcoRII	CCWGG	5 cut(s)	76, 175, 298, 589, 622
EcoT14I	CCWWGG	1 cut(s)	70
ErhI	CCWWGG	1 cut(s)	70
FaeI	CATG	6 cut(s)	234, 355, 514, 637, 649, 683
FaiI	YATR	8 cut(s)	232, 275, 353, 512, 551, 635, 647, 681
FaqI	GGGAC	1 cut(s)	266
FatI	CATG	6 cut(s)	230, 351, 510, 633, 645, 679
Fnu4HI	GCNGC	5 cut(s)	245, 327, 449, 497, 500
FokI	GGATG	1 cut(s)	193
Fsp4HI	GCNGC	5 cut(s)	245, 327, 449, 497, 500
FspBI	CTAG	2 cut(s)	165, 219
GlaI	GCGC	1 cut(s)	576
GluI	GCNGC	5 cut(s)	245, 327, 449, 497, 500
GsaI	CCCAGC	1 cut(s)	542
GsuI	CTGGAG	3 cut(s)	109, 153, 512
HaeIII	GGCC	3 cut(s)	91, 377, 582
HhaI	GCGC	1 cut(s)	577
Hin1II	CATG	6 cut(s)	234, 355, 514, 637, 649, 683
Hin6I	GCGC	1 cut(s)	575
HinP1I	GCGC	1 cut(s)	575
HinfI	GANTC	2 cut(s)	203, 392
HphI	GGTGA	1 cut(s)	367
Hpy188I	TCNGA	1 cut(s)	160
Hpy188III	TCNNGA	2 cut(s)	410, 674
Hpy99I	CGWCG	1 cut(s)	252
HpyAV	CCTTC	2 cut(s)	382, 695
HpyCH4V	TGCA	1 cut(s)	287
HpyF10VI	GCNNNNNNNGC	4 cut(s)	97, 496, 515, 646
HpyF3I	CTNAG	3 cut(s)	157, 435, 672
Hsp92II	CATG	6 cut(s)	234, 355, 514, 637, 649, 683
HspAI	GCGC	1 cut(s)	575
Kzo9I	GATC	3 cut(s)	39, 179, 676
LmnI	GCTCC	4 cut(s)	295, 493, 523, 592
Lsp1109I	GCAGC	5 cut(s)	256, 313, 435, 508, 511
LweI	GCATC	3 cut(s)	34, 331, 543
MaeI	CTAG	2 cut(s)	165, 219
MaeIII	GTNAC	4 cut(s)	6, 32, 355, 617
MalI	GATC	3 cut(s)	41, 181, 678
MboI	GATC	3 cut(s)	39, 179, 676
MboII	GAAGA	3 cut(s)	108, 398, 484
MflI	RGATCY	1 cut(s)	179
MluCI	AATT	4 cut(s)	22, 278, 288, 397
MlyI	GAGTC	1 cut(s)	386
MnlI	CCTC	8 cut(s)	183, 220, 318, 367, 459, 531, 660, 702
MseI	TTAA	2 cut(s)	428, 703
MspA1I	CMGCKG	1 cut(s)	502
MspR9I	CCNGG	5 cut(s)	78, 177, 300, 591, 624
Mva1269I	GAATGC	1 cut(s)	520
MvaI	CCWGG	5 cut(s)	78, 177, 300, 591, 624
MwoI	GCNNNNNNNGC	4 cut(s)	97, 496, 515, 646
NdeII	GATC	3 cut(s)	39, 179, 676
NheI	GCTAGC	1 cut(s)	164
NlaIII	CATG	6 cut(s)	234, 355, 514, 637, 649, 683
NlaIV	GGNNCC	3 cut(s)	181, 297, 606
NmeAIII	GCCGAG	1 cut(s)	125
NmuCI	GTSAC	3 cut(s)	6, 32, 355
NspI	RCATGY	1 cut(s)	234
PctI	GAATGC	1 cut(s)	520
PfeI	GAWTC	1 cut(s)	203
PfoI	TCCNGGA	1 cut(s)	589
PkrI	GCNGC	5 cut(s)	246, 328, 450, 498, 501
PleI	GAGTC	1 cut(s)	386
PpsI	GAGTC	1 cut(s)	386
PpuMI	RGGWCCY	1 cut(s)	74
Psp5II	RGGWCCY	1 cut(s)	74
Psp6I	CCWGG	5 cut(s)	76, 175, 298, 589, 622
PspFI	CCCAGC	1 cut(s)	538
PspGI	CCWGG	5 cut(s)	76, 175, 298, 589, 622
PspN4I	GGNNCC	3 cut(s)	181, 297, 606
PspPI	GGNCC	2 cut(s)	74, 376
PspPPI	RGGWCCY	1 cut(s)	74
PstNI	CAGNNNCTG	3 cut(s)	385, 469, 502
PsuI	RGATCY	1 cut(s)	179
PvuII	CAGCTG	1 cut(s)	502
SaqAI	TTAA	2 cut(s)	428, 703
SatI	GCNGC	5 cut(s)	245, 327, 449, 497, 500
Sau3AI	GATC	3 cut(s)	39, 179, 676
Sau96I	GGNCC	2 cut(s)	74, 376
SchI	GAGTC	1 cut(s)	386
ScrFI	CCNGG	5 cut(s)	78, 177, 300, 591, 624
SfaNI	GCATC	3 cut(s)	34, 331, 543
SinI	GGWCC	1 cut(s)	74
Sse9I	AATT	4 cut(s)	22, 278, 288, 397
SsiI	CCGC	1 cut(s)	349
SspMI	CTAG	2 cut(s)	165, 219
StyD4I	CCNGG	5 cut(s)	76, 175, 298, 589, 622
StyI	CCWWGG	1 cut(s)	70
TaqI	TCGA	3 cut(s)	42, 224, 663
TasI	AATT	4 cut(s)	22, 278, 288, 397
TfiI	GAWTC	1 cut(s)	203
Tru1I	TTAA	2 cut(s)	428, 703
Tru9I	TTAA	2 cut(s)	428, 703
TscAI	CASTG	1 cut(s)	133
TseFI	GTSAC	3 cut(s)	6, 32, 355
TseI	GCWGC	5 cut(s)	244, 326, 448, 496, 499
Tsp45I	GTSAC	3 cut(s)	6, 32, 355
TspDTI	ATGAA	2 cut(s)	527, 634
TspGWI	ACGGA	1 cut(s)	69
TspRI	CASTG	1 cut(s)	133
VpaK11BI	GGWCC	1 cut(s)	74
XceI	RCATGY	1 cut(s)	234
XspI	CTAG	2 cut(s)	165, 219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.