Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003582

Physical Location & Seq

Genomic Coordinates Reverse (-)

33143686 .. 33146605

Gene Structure 2920 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1227800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 690 bp

ATGGCCTCCCCGGAAAACACCAATTGGCTCTTCGATTACGGGCTCATTGACGATACCCCTGTCCTGGATGGGAACTTCGCTTGGCCAGTTCAGCCCATCGCCGGTTCATCCAGTGCCAGTGTTGAACTTGATGGGTCATTGGGGGATGCAGAGGGACTTAAGGAATCTGGCTCGAAAAAGAGGGTCAGAACTGAATCATGCAGTGGAACAAGCTCCAAGGCATGCAGGGAGAAGTTGCGAAGGGATAGGCTAAATGACAAGTTTGTGGAATTGGGCTCTATTTTGGAGCCTGGAAGACCCCCCAAGACGGACAAGGCTGCTATTTTGGTTGATGCTGTACGAATGGTGAATCAATTACGTGGTGAAGCCCAAAAGTTGAAGGACACAAATTCAGGCCTCCAGGAGAAGATCAAGGAATTGAAGGCAGAGAAGAATGAACTTCGCGATGAGAAGCAGAGGCTGAAATCGGAGAAAGAGAAGTTGGAACAGCAACTTAAATCCATGAATATTCAGCCCGGCTTTTTACCTCCCCCTCCTGCAATTCCTGCTGCATTTGCTGCTCAAGGCCAAGCTCGTGGAAACAAGATGGTGCCTTTCATTGGCTACCCTGGGGTCGCCATGTGGCAGTTCATGCCACCTGCCGCGGTGGATACTTCGCAGGATCATGTACTCCGCCCACCGGTTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

230

Amino Acids

25.09

Weight (kDa)

8.45

Isoelectric Point (pI)

48.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	72 - 120	8.1e-10	Helix-loop-helix DNA-binding domain
bHLH_IRO3	PF23177	73 - 136	2.9e-10	Iron-related transcription factor 3 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54680
fragaria_vesca	FvH4_3g02910 FvH4_3g02910 FvH4_3g21720
malus_domestica	MD03G1212600.v1.1 MD05G1336500.v1.1 MD05G1336600.v1.1 MD10G1312000.v1.1 MD10G1312100.v1.1 MD11G1227800.v1.1
prunus_persica	Prupe.4G029000_v2.0.a1 Prupe.4G201500_v2.0.a1
pyrus_communis	pycom03g16130 pycom05g30800 pycom10g26330 pycom11g20040
rosa_chinensis	RchiOBHm_Chr5g0004471 RchiOBHm_Chr5g0037201
rosa_laevigata	RLG00000031231 RLG00000033764
rosa_multiflora	Rmu_sc0000433.1_g000001 Rmu_sc0002453.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043770 Rroxscaffold_1G00071050
rosa_rugosa	Rorug04G0412000 Rorug05G0163600
rosa_samantha	Rh5AG039600 Rh5AG253200 Rh5BG038600 Rh5BG255200 Rh5CG042700 Rh5CG287900 Rh5DG038400
rosa_wichuraiana	Rw5G003780 Rw5G023390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	646
Acc36I	ACCTGC	1 cut(s)	646
AccB1I	GGYRCC	1 cut(s)	589
AccII	CGCG	2 cut(s)	444, 644
AciI	CCGC	3 cut(s)	642, 644, 673
AclWI	GGATC	1 cut(s)	669
AcoI	YGGCCR	1 cut(s)	83
AcsI	RAATTY	1 cut(s)	388
AfaI	GTAC	2 cut(s)	339, 669
AfiI	CCNNNNNNNGG	5 cut(s)	64, 101, 307, 599, 679
AflII	CTTAAG	1 cut(s)	158
AgeI	ACCGGT	1 cut(s)	679
AgsI	TTSAA	3 cut(s)	125, 379, 421
AjnI	CCWGG	4 cut(s)	63, 289, 399, 607
AjuI	GAANNNNNNNTTGG	4 cut(s)	14, 46, 464, 496
AluBI	AGCT	2 cut(s)	213, 572
AluI	AGCT	2 cut(s)	213, 572
AlwI	GGATC	1 cut(s)	669
AlwNI	CAGNNNCTG	1 cut(s)	460
AoxI	GGCC	4 cut(s)	3, 83, 394, 565
ApeKI	GCWGC	3 cut(s)	317, 548, 557
ApoI	RAATTY	1 cut(s)	388
AsiGI	ACCGGT	1 cut(s)	679
AsuC2I	CCSGG	2 cut(s)	11, 516
AsuHPI	GGTGA	2 cut(s)	358, 374
BaeI	ACNNNNGTAYC	2 cut(s)	45, 78
BalI	TGGCCA	1 cut(s)	85
BanI	GGYRCC	1 cut(s)	589
BanII	GRGCYC	2 cut(s)	45, 278
BauI	CACGAG	1 cut(s)	573
BbsI	GAAGAC	1 cut(s)	301
BbvI	GCAGC	3 cut(s)	304, 535, 544
BccI	CCATC	4 cut(s)	62, 104, 125, 580
BciT130I	CCWGG	4 cut(s)	65, 291, 401, 609
BciVI	GTATCC	1 cut(s)	643
BcnI	CCSGG	2 cut(s)	11, 516
BfrI	CTTAAG	1 cut(s)	158
BfuAI	ACCTGC	1 cut(s)	646
BfuI	GTATCC	1 cut(s)	643
BisI	GCNGC	4 cut(s)	318, 549, 558, 642
BlsI	GCNGC	4 cut(s)	319, 550, 559, 643
Bme1390I	CCNGG	6 cut(s)	11, 65, 291, 401, 516, 609
BmiI	GGNNCC	2 cut(s)	288, 591
BmrFI	CCNGG	6 cut(s)	11, 65, 291, 401, 516, 609
BmsI	GCATC	2 cut(s)	136, 322
BpiI	GAAGAC	1 cut(s)	301
BpmI	CTGGAG	1 cut(s)	383
BpuEI	CTTGAG	1 cut(s)	546
BpuMI	CCSGG	2 cut(s)	11, 516
BsaAI	YACGTR	1 cut(s)	359
BsaJI	CCNNGG	5 cut(s)	9, 216, 607, 608, 642
BsaWI	WCCGGW	1 cut(s)	679
Bsc4I	CCNNNNNNNGG	5 cut(s)	64, 101, 307, 599, 679
Bse118I	RCCGGY	2 cut(s)	101, 679
Bse1I	ACTGG	3 cut(s)	86, 111, 117
BseBI	CCWGG	4 cut(s)	65, 291, 401, 609
BseDI	CCNNGG	5 cut(s)	9, 216, 607, 608, 642
BseGI	GGATG	3 cut(s)	73, 107, 151
BseLI	CCNNNNNNNGG	5 cut(s)	64, 101, 307, 599, 679
BseNI	ACTGG	3 cut(s)	86, 111, 117
BseXI	GCAGC	3 cut(s)	304, 535, 544
Bsh1236I	CGCG	2 cut(s)	444, 644
BshFI	GGCC	4 cut(s)	5, 85, 396, 567
BshNI	GGYRCC	1 cut(s)	589
BshTI	ACCGGT	1 cut(s)	679
BsiSI	CCGG	4 cut(s)	11, 102, 516, 680
BslFI	GGGAC	1 cut(s)	168
BslI	CCNNNNNNNGG	5 cut(s)	64, 101, 307, 599, 679
BsmFI	GGGAC	1 cut(s)	168
BsnI	GGCC	4 cut(s)	5, 85, 396, 567
Bsp1286I	GDGCHC	2 cut(s)	45, 278
Bsp143I	GATC	2 cut(s)	408, 661
Bsp68I	TCGCGA	1 cut(s)	444
BspACI	CCGC	3 cut(s)	642, 644, 673
BspANI	GGCC	4 cut(s)	5, 85, 396, 567
BspFNI	CGCG	2 cut(s)	444, 644
BspLI	GGNNCC	2 cut(s)	288, 591
BspMI	ACCTGC	1 cut(s)	646
BspPI	GGATC	1 cut(s)	669
BspQI	GCTCTTC	1 cut(s)	35
BspT107I	GGYRCC	1 cut(s)	589
BspTI	CTTAAG	1 cut(s)	158
BsrFI	RCCGGY	2 cut(s)	101, 679
BsrI	ACTGG	3 cut(s)	86, 111, 117
BssAI	RCCGGY	2 cut(s)	101, 679
BssECI	CCNNGG	5 cut(s)	9, 216, 607, 608, 642
BssMI	GATC	2 cut(s)	408, 661
BssSI	CACGAG	1 cut(s)	573
BssT1I	CCWWGG	1 cut(s)	216
Bst2BI	CACGAG	1 cut(s)	573
Bst2UI	CCWGG	4 cut(s)	65, 291, 401, 609
Bst6I	CTCTTC	1 cut(s)	35
BstAFI	CTTAAG	1 cut(s)	158
BstAPI	GCANNNNNTGC	3 cut(s)	545, 557, 631
BstBAI	YACGTR	1 cut(s)	359
BstC8I	GCNNGC	1 cut(s)	223
BstDSI	CCRYGG	1 cut(s)	642
BstF5I	GGATG	3 cut(s)	73, 107, 151
BstFNI	CGCG	2 cut(s)	444, 644
BstKTI	GATC	2 cut(s)	411, 664
BstMBI	GATC	2 cut(s)	408, 661
BstMWI	GCNNNNNNNGC	5 cut(s)	91, 545, 554, 557, 631
BstNI	CCWGG	4 cut(s)	65, 291, 401, 609
BstNSI	RCATGY	1 cut(s)	225
BstSCI	CCNGG	6 cut(s)	9, 63, 289, 399, 514, 607
BstUI	CGCG	2 cut(s)	444, 644
BstV1I	GCAGC	3 cut(s)	304, 535, 544
BstV2I	GAAGAC	1 cut(s)	301
BstXI	CCANNNNNNTGG	1 cut(s)	575
BsuI	GTATCC	1 cut(s)	643
BsuRI	GGCC	4 cut(s)	5, 85, 396, 567
BtgI	CCRYGG	1 cut(s)	642
BtgZI	GCGATG	2 cut(s)	82, 459
BtsCI	GGATG	3 cut(s)	73, 107, 151
BtsI	GCAGTG	1 cut(s)	208
BtsIMutI	CAGTG	3 cut(s)	118, 124, 208
BtuMI	TCGCGA	1 cut(s)	444
BveI	ACCTGC	1 cut(s)	646
Cac8I	GCNNGC	1 cut(s)	223
CaiI	CAGNNNCTG	1 cut(s)	460
Cfr10I	RCCGGY	2 cut(s)	101, 679
Cfr42I	CCGCGG	1 cut(s)	645
Csp6I	GTAC	2 cut(s)	338, 668
CspAI	ACCGGT	1 cut(s)	679
CviAII	CATG	6 cut(s)	198, 222, 502, 619, 631, 665
CviQI	GTAC	2 cut(s)	338, 668
DpnI	GATC	2 cut(s)	410, 663
DpnII	GATC	2 cut(s)	408, 661
EaeI	YGGCCR	1 cut(s)	83
Eam1104I	CTCTTC	1 cut(s)	35
EarI	CTCTTC	1 cut(s)	35
EciI	GGCGGA	1 cut(s)	662
Eco130I	CCWWGG	1 cut(s)	216
Eco147I	AGGCCT	1 cut(s)	396
Eco24I	GRGCYC	2 cut(s)	45, 278
EcoRII	CCWGG	4 cut(s)	63, 289, 399, 607
EcoT14I	CCWWGG	1 cut(s)	216
EcoT38I	GRGCYC	2 cut(s)	45, 278
ErhI	CCWWGG	1 cut(s)	216
FaeI	CATG	6 cut(s)	201, 225, 505, 622, 634, 668
FaiI	YATR	6 cut(s)	199, 223, 503, 620, 632, 666
FaqI	GGGAC	1 cut(s)	168
FatI	CATG	6 cut(s)	197, 221, 501, 618, 630, 664
Fnu4HI	GCNGC	4 cut(s)	318, 549, 558, 642
FokI	GGATG	3 cut(s)	80, 94, 158
FriOI	GRGCYC	2 cut(s)	45, 278
Fsp4HI	GCNGC	4 cut(s)	318, 549, 558, 642
GluI	GCNGC	4 cut(s)	318, 549, 558, 642
GsuI	CTGGAG	1 cut(s)	383
HaeIII	GGCC	4 cut(s)	5, 85, 396, 567
HapII	CCGG	4 cut(s)	11, 102, 516, 680
Hin1II	CATG	6 cut(s)	201, 225, 505, 622, 634, 668
HinfI	GANTC	3 cut(s)	164, 194, 349
HpaII	CCGG	4 cut(s)	11, 102, 516, 680
HphI	GGTGA	2 cut(s)	358, 374
Hpy188I	TCNGA	2 cut(s)	188, 469
Hpy188III	TCNNGA	1 cut(s)	443
HpyAV	CCTTC	3 cut(s)	234, 373, 415
HpyCH4IV	ACGT	1 cut(s)	358
HpyCH4V	TGCA	5 cut(s)	149, 201, 225, 539, 551
HpyF10VI	GCNNNNNNNGC	5 cut(s)	91, 545, 554, 557, 631
HpySE526I	ACGT	1 cut(s)	358
Hsp92II	CATG	6 cut(s)	201, 225, 505, 622, 634, 668
KspI	CCGCGG	1 cut(s)	645
Kzo9I	GATC	2 cut(s)	408, 661
LguI	GCTCTTC	1 cut(s)	35
LmnI	GCTCC	2 cut(s)	218, 286
Lsp1109I	GCAGC	3 cut(s)	304, 535, 544
LweI	GCATC	2 cut(s)	136, 322
MaeII	ACGT	1 cut(s)	358
MalI	GATC	2 cut(s)	410, 663
MboI	GATC	2 cut(s)	408, 661
MboII	GAAGA	4 cut(s)	22, 306, 418, 442
MfeI	CAATTG	1 cut(s)	22
MhlI	GDGCHC	2 cut(s)	45, 278
MlsI	TGGCCA	1 cut(s)	85
MluCI	AATT	6 cut(s)	22, 269, 353, 388, 416, 540
MluNI	TGGCCA	1 cut(s)	85
MmeI	TCCRAC	1 cut(s)	462
MnlI	CCTC	7 cut(s)	16, 145, 174, 407, 450, 537, 543
Mox20I	TGGCCA	1 cut(s)	85
MscI	TGGCCA	1 cut(s)	85
MseI	TTAA	3 cut(s)	159, 495, 688
Msp20I	TGGCCA	1 cut(s)	85
MspA1I	CMGCKG	1 cut(s)	644
MspCI	CTTAAG	1 cut(s)	158
MspI	CCGG	4 cut(s)	11, 102, 516, 680
MspR9I	CCNGG	6 cut(s)	11, 65, 291, 401, 516, 609
MunI	CAATTG	1 cut(s)	22
MvaI	CCWGG	4 cut(s)	65, 291, 401, 609
MvnI	CGCG	2 cut(s)	444, 644
MwoI	GCNNNNNNNGC	5 cut(s)	91, 545, 554, 557, 631
NciI	CCSGG	2 cut(s)	11, 516
NdeII	GATC	2 cut(s)	408, 661
NlaIII	CATG	6 cut(s)	201, 225, 505, 622, 634, 668
NlaIV	GGNNCC	2 cut(s)	288, 591
NruI	TCGCGA	1 cut(s)	444
NspI	RCATGY	1 cut(s)	225
PaeI	GCATGC	1 cut(s)	225
PaqCI	CACCTGC	1 cut(s)	646
PasI	CCCWGGG	1 cut(s)	608
PceI	AGGCCT	1 cut(s)	396
PciSI	GCTCTTC	1 cut(s)	35
PfeI	GAWTC	3 cut(s)	164, 194, 349
PfoI	TCCNGGA	2 cut(s)	63, 399
PinAI	ACCGGT	1 cut(s)	679
PkrI	GCNGC	4 cut(s)	319, 550, 559, 643
Ppu21I	YACGTR	1 cut(s)	359
Psp6I	CCWGG	4 cut(s)	63, 289, 399, 607
PspGI	CCWGG	4 cut(s)	63, 289, 399, 607
PspN4I	GGNNCC	2 cut(s)	288, 591
PstNI	CAGNNNCTG	1 cut(s)	460
RruI	TCGCGA	1 cut(s)	444
RsaI	GTAC	2 cut(s)	339, 669
RsaNI	GTAC	2 cut(s)	338, 668
SacII	CCGCGG	1 cut(s)	645
SapI	GCTCTTC	1 cut(s)	35
SaqAI	TTAA	3 cut(s)	159, 495, 688
SatI	GCNGC	4 cut(s)	318, 549, 558, 642
Sau3AI	GATC	2 cut(s)	408, 661
ScrFI	CCNGG	6 cut(s)	11, 65, 291, 401, 516, 609
SduI	GDGCHC	2 cut(s)	45, 278
SetI	ASST	5 cut(s)	215, 361, 529, 574, 640
SfaNI	GCATC	2 cut(s)	136, 322
Sfr303I	CCGCGG	1 cut(s)	645
SgrBI	CCGCGG	1 cut(s)	645
SmlI	CTYRAG	2 cut(s)	158, 561
SmoI	CTYRAG	2 cut(s)	158, 561
SphI	GCATGC	1 cut(s)	225
Sse9I	AATT	6 cut(s)	22, 269, 353, 388, 416, 540
SseBI	AGGCCT	1 cut(s)	396
SsiI	CCGC	3 cut(s)	642, 644, 673
SspI	AATATT	1 cut(s)	508
StuI	AGGCCT	1 cut(s)	396
StyD4I	CCNGG	6 cut(s)	9, 63, 289, 399, 514, 607
StyI	CCWWGG	1 cut(s)	216
TaiI	ACGT	1 cut(s)	361
TaqI	TCGA	2 cut(s)	33, 173
TasI	AATT	6 cut(s)	22, 269, 353, 388, 416, 540
TatI	WGTACW	1 cut(s)	667
TauI	GCSGC	1 cut(s)	644
TfiI	GAWTC	3 cut(s)	164, 194, 349
Tru1I	TTAA	3 cut(s)	159, 495, 688
Tru9I	TTAA	3 cut(s)	159, 495, 688
TscAI	CASTG	3 cut(s)	118, 124, 208
TseI	GCWGC	3 cut(s)	317, 548, 557
TspDTI	ATGAA	5 cut(s)	96, 450, 518, 586, 619
TspGWI	ACGGA	1 cut(s)	323
TspRI	CASTG	3 cut(s)	118, 124, 208
Vha464I	CTTAAG	1 cut(s)	158
XapI	RAATTY	1 cut(s)	388
XceI	RCATGY	1 cut(s)	225

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.