MD03G1212600.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003582
Physical Location & Seq
Reverse (-)
29176577 .. 29179687
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1212600.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGTCTCCCCGGAAAACACCAACTGGCTCTTCGATTACGGGCTCATCGACGATACCCCTGTTCTGGATGGCAACTTCACTTGGCCCGTTCAGCCCGTCGCCGGTTCGGCTAGCGTCAGTGTTGAACTTGATGGCTCATTGGGGGATGCTGAGGGACTTAAGGAATCTGGCTCAAAAAAGAGGGTCAGATCTGAATCATGCAGTGGAACAAGCTCGAAGGCATGTAGGGAGAAGTTGCGAAGGGACAGGCTAAATGACAAGTTTGTGGAATTGGGCTCTATTTTGGAGCCTGGAAGGCCCCCCAAGACGGACAAGGCTGCCATTCTGGTTGATGCTGTACGAATGGTGAATCAATTACGTGGTGAAGCCCAGAAGTTGAAGGACACAAATTCAGGCCTCCAGGAGAAGATCAAGGAATTGAAGACAGAGAAGAATGAACTTCGCGATGAGAAGCAGAGGCTTAAATCGGAGAAAGAGAAGTTGGAACAGCAACTGAAATCCATGAATGCGCAGCCTGGCTTTTTGCCTCCCCCTCCTGGAATTCCTGCTGCATTTGCGGCTCAAGGCCAAGCCCACGGCAACAAGTTGGTGCCTTTCATTGGTTACCCTGGGGTTGCCATGTGGCAATTCATGCCACCTGCCGCTGTGGATACTTCGCAGGATCATGTACTCCGCCCACCGGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

230

Amino Acids

25.07

Weight (kDa)

7.71

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 72 - 120 8.1e-10 Helix-loop-helix DNA-binding domain
bHLH_IRO3 PF23177 73 - 136 2.9e-10 Iron-related transcription factor 3 bHLH domain
CCDC113_CCDC96_CC PF13870 113 - 173 9.3e-06 CCDC113/CCDC96, coiled-coil
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 646
Acc16I TGCGCA 1 cut(s) 510
Acc36I ACCTGC 1 cut(s) 646
AccB1I GGYRCC 1 cut(s) 589
AccII CGCG 1 cut(s) 444
AciI CCGC 3 cut(s) 557, 642, 673
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 2 cut(s) 388, 540
AfaI GTAC 2 cut(s) 339, 669
AfiI CCNNNNNNNGG 6 cut(s) 64, 101, 307, 536, 599, 679
AflII CTTAAG 1 cut(s) 158
AgeI ACCGGT 1 cut(s) 679
AgsI TTSAA 3 cut(s) 125, 379, 421
AjnI CCWGG 5 cut(s) 289, 399, 514, 535, 607
AjuI GAANNNNNNNTTGG 4 cut(s) 14, 46, 464, 496
AluBI AGCT 1 cut(s) 213
AluI AGCT 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 1 cut(s) 669
AlwNI CAGNNNCTG 1 cut(s) 493
AoxI GGCC 4 cut(s) 83, 296, 394, 565
ApeKI GCWGC 3 cut(s) 317, 511, 548
ApoI RAATTY 2 cut(s) 388, 540
AsiGI ACCGGT 1 cut(s) 679
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 2 cut(s) 84, 297
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 2 cut(s) 358, 374
AsuNHI GCTAGC 1 cut(s) 110
BaeI ACNNNNGTAYC 2 cut(s) 45, 78
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 45, 278
BbsI GAAGAC 1 cut(s) 428
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 304, 523, 535
BccI CCATC 2 cut(s) 62, 125
BceAI ACGGC 1 cut(s) 592
BciT130I CCWGG 5 cut(s) 291, 401, 516, 537, 609
BciVI GTATCC 1 cut(s) 643
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 1 cut(s) 111
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 646
BfuI GTATCC 1 cut(s) 643
BglI GCCNNNNNGGC 2 cut(s) 107, 295
BglII AGATCT 1 cut(s) 188
BisI GCNGC 5 cut(s) 318, 512, 549, 558, 642
BlsI GCNGC 5 cut(s) 319, 513, 550, 559, 643
Bme1390I CCNGG 6 cut(s) 11, 291, 401, 516, 537, 609
BmgT120I GGNCC 2 cut(s) 84, 297
BmiI GGNNCC 3 cut(s) 288, 299, 591
BmrFI CCNGG 6 cut(s) 11, 291, 401, 516, 537, 609
BmsI GCATC 2 cut(s) 136, 322
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 1 cut(s) 383
Bpu10I CCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 11
BsaAI YACGTR 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 4 cut(s) 9, 574, 607, 608
BsaWI WCCGGW 1 cut(s) 679
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 101, 307, 536, 599, 679
Bse118I RCCGGY 2 cut(s) 101, 679
Bse1I ACTGG 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 193
BseBI CCWGG 5 cut(s) 291, 401, 516, 537, 609
BseDI CCNNGG 4 cut(s) 9, 574, 607, 608
BseGI GGATG 2 cut(s) 73, 151
BseJI GATNNNNATC 1 cut(s) 193
BseLI CCNNNNNNNGG 6 cut(s) 64, 101, 307, 536, 599, 679
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 304, 523, 535
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 4 cut(s) 85, 298, 396, 567
BshNI GGYRCC 1 cut(s) 589
BshTI ACCGGT 1 cut(s) 679
BsiSI CCGG 3 cut(s) 11, 102, 680
BslFI GGGAC 2 cut(s) 168, 257
BslI CCNNNNNNNGG 6 cut(s) 64, 101, 307, 536, 599, 679
BsmAI GTCTC 1 cut(s) 10
BsmFI GGGAC 2 cut(s) 168, 257
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 4 cut(s) 85, 298, 396, 567
Bso31I GGTCTC 1 cut(s) 10
Bsp1286I GDGCHC 2 cut(s) 45, 278
Bsp143I GATC 3 cut(s) 188, 408, 661
Bsp68I TCGCGA 1 cut(s) 444
BspACI CCGC 3 cut(s) 557, 642, 673
BspANI GGCC 4 cut(s) 85, 298, 396, 567
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 3 cut(s) 288, 299, 591
BspMI ACCTGC 1 cut(s) 646
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 669
BspQI GCTCTTC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 589
BspTI CTTAAG 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 10
BsrFI RCCGGY 2 cut(s) 101, 679
BsrI ACTGG 1 cut(s) 29
BssAI RCCGGY 2 cut(s) 101, 679
BssECI CCNNGG 4 cut(s) 9, 574, 607, 608
BssMI GATC 3 cut(s) 188, 408, 661
Bst2UI CCWGG 5 cut(s) 291, 401, 516, 537, 609
Bst6I CTCTTC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 631
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 574
BstEII GGTNACC 1 cut(s) 602
BstF5I GGATG 2 cut(s) 73, 151
BstFNI CGCG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 3 cut(s) 191, 411, 664
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 3 cut(s) 188, 408, 661
BstMWI GCNNNNNNNGC 6 cut(s) 91, 107, 295, 554, 557, 631
BstNI CCWGG 5 cut(s) 291, 401, 516, 537, 609
BstNSI RCATGY 1 cut(s) 225
BstPI GGTNACC 1 cut(s) 602
BstSCI CCNGG 6 cut(s) 9, 289, 399, 514, 535, 607
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 3 cut(s) 304, 523, 535
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BsuI GTATCC 1 cut(s) 643
BsuRI GGCC 4 cut(s) 85, 298, 396, 567
BtgI CCRYGG 1 cut(s) 574
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 2 cut(s) 73, 151
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 124, 208
BtuMI TCGCGA 1 cut(s) 444
BveI ACCTGC 1 cut(s) 646
Cac8I GCNNGC 1 cut(s) 112
CaiI CAGNNNCTG 1 cut(s) 493
CfoI GCGC 1 cut(s) 511
Cfr10I RCCGGY 2 cut(s) 101, 679
Cfr13I GGNCC 2 cut(s) 84, 297
CseI GACGC 1 cut(s) 103
Csp6I GTAC 2 cut(s) 338, 668
CspAI ACCGGT 1 cut(s) 679
CviAII CATG 6 cut(s) 198, 222, 502, 619, 631, 665
CviQI GTAC 2 cut(s) 338, 668
DdeI CTNAG 1 cut(s) 150
DpnI GATC 3 cut(s) 190, 410, 663
DpnII GATC 3 cut(s) 188, 408, 661
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 1 cut(s) 662
Eco147I AGGCCT 1 cut(s) 396
Eco24I GRGCYC 2 cut(s) 45, 278
Eco31I GGTCTC 1 cut(s) 10
Eco91I GGTNACC 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 297
EcoO65I GGTNACC 1 cut(s) 602
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 5 cut(s) 289, 399, 514, 535, 607
EcoT38I GRGCYC 2 cut(s) 45, 278
FaeI CATG 6 cut(s) 201, 225, 505, 622, 634, 668
FaiI YATR 6 cut(s) 199, 223, 503, 620, 632, 666
FaqI GGGAC 2 cut(s) 168, 257
FatI CATG 6 cut(s) 197, 221, 501, 618, 630, 664
Fnu4HI GCNGC 5 cut(s) 318, 512, 549, 558, 642
FokI GGATG 2 cut(s) 80, 158
FriOI GRGCYC 2 cut(s) 45, 278
Fsp4HI GCNGC 5 cut(s) 318, 512, 549, 558, 642
FspBI CTAG 1 cut(s) 111
FspI TGCGCA 1 cut(s) 510
GlaI GCGC 1 cut(s) 510
GluI GCNGC 5 cut(s) 318, 512, 549, 558, 642
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 4 cut(s) 85, 298, 396, 567
HapII CCGG 3 cut(s) 11, 102, 680
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 511
Hin1II CATG 6 cut(s) 201, 225, 505, 622, 634, 668
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HinfI GANTC 3 cut(s) 164, 194, 349
HpaII CCGG 3 cut(s) 11, 102, 680
HphI GGTGA 2 cut(s) 358, 374
Hpy188I TCNGA 3 cut(s) 188, 193, 469
Hpy188III TCNNGA 2 cut(s) 65, 443
Hpy99I CGWCG 2 cut(s) 53, 101
HpyAV CCTTC 4 cut(s) 211, 234, 288, 373
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 2 cut(s) 201, 551
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 107, 295, 554, 557, 631
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 1 cut(s) 358
Hsp92II CATG 6 cut(s) 201, 225, 505, 622, 634, 668
HspAI GCGC 1 cut(s) 509
Kzo9I GATC 3 cut(s) 188, 408, 661
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 3 cut(s) 304, 523, 535
LweI GCATC 2 cut(s) 136, 322
MaeI CTAG 1 cut(s) 111
MaeII ACGT 1 cut(s) 358
MaeIII GTNAC 1 cut(s) 602
MalI GATC 3 cut(s) 190, 410, 663
MboI GATC 3 cut(s) 188, 408, 661
MboII GAAGA 4 cut(s) 22, 418, 433, 442
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 2 cut(s) 45, 278
MluCI AATT 6 cut(s) 269, 353, 388, 416, 540, 626
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 6 cut(s) 145, 174, 407, 450, 537, 543
MseI TTAA 3 cut(s) 159, 462, 688
MspA1I CMGCKG 1 cut(s) 644
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 3 cut(s) 11, 102, 680
MspR9I CCNGG 6 cut(s) 11, 291, 401, 516, 537, 609
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 5 cut(s) 291, 401, 516, 537, 609
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 6 cut(s) 91, 107, 295, 554, 557, 631
NciI CCSGG 1 cut(s) 11
NdeII GATC 3 cut(s) 188, 408, 661
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 6 cut(s) 201, 225, 505, 622, 634, 668
NlaIV GGNNCC 3 cut(s) 288, 299, 591
NruI TCGCGA 1 cut(s) 444
NsbI TGCGCA 1 cut(s) 510
NspI RCATGY 1 cut(s) 225
PaqCI CACCTGC 1 cut(s) 646
PasI CCCWGGG 1 cut(s) 608
PceI AGGCCT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 511
PfeI GAWTC 3 cut(s) 164, 194, 349
PfoI TCCNGGA 2 cut(s) 399, 535
PinAI ACCGGT 1 cut(s) 679
PkrI GCNGC 5 cut(s) 319, 513, 550, 559, 643
Ppu21I YACGTR 1 cut(s) 359
Psp6I CCWGG 5 cut(s) 289, 399, 514, 535, 607
PspEI GGTNACC 1 cut(s) 602
PspGI CCWGG 5 cut(s) 289, 399, 514, 535, 607
PspN4I GGNNCC 3 cut(s) 288, 299, 591
PspPI GGNCC 2 cut(s) 84, 297
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 188
RruI TCGCGA 1 cut(s) 444
RsaI GTAC 2 cut(s) 339, 669
RsaNI GTAC 2 cut(s) 338, 668
SapI GCTCTTC 1 cut(s) 35
SaqAI TTAA 3 cut(s) 159, 462, 688
SatI GCNGC 5 cut(s) 318, 512, 549, 558, 642
Sau3AI GATC 3 cut(s) 188, 408, 661
Sau96I GGNCC 2 cut(s) 84, 297
ScrFI CCNGG 6 cut(s) 11, 291, 401, 516, 537, 609
SduI GDGCHC 2 cut(s) 45, 278
SetI ASST 3 cut(s) 215, 361, 640
SfaNI GCATC 2 cut(s) 136, 322
SmlI CTYRAG 2 cut(s) 158, 561
SmoI CTYRAG 2 cut(s) 158, 561
Sse9I AATT 6 cut(s) 269, 353, 388, 416, 540, 626
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 3 cut(s) 557, 642, 673
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 6 cut(s) 9, 289, 399, 514, 535, 607
TaiI ACGT 1 cut(s) 361
TaqI TCGA 3 cut(s) 33, 48, 215
TasI AATT 6 cut(s) 269, 353, 388, 416, 540, 626
TatI WGTACW 1 cut(s) 667
TauI GCSGC 2 cut(s) 560, 644
TfiI GAWTC 3 cut(s) 164, 194, 349
Tru1I TTAA 3 cut(s) 159, 462, 688
Tru9I TTAA 3 cut(s) 159, 462, 688
TscAI CASTG 2 cut(s) 124, 208
TseI GCWGC 3 cut(s) 317, 511, 548
TspDTI ATGAA 4 cut(s) 450, 518, 586, 619
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 2 cut(s) 124, 208
Vha464I CTTAAG 1 cut(s) 158
XapI RAATTY 2 cut(s) 388, 540
XceI RCATGY 1 cut(s) 225
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.