pycom03g16130

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003582
Physical Location & Seq
Reverse (-)
17288102 .. 17290942
2841 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16130.4

Sequence Viewer

Length: 747 bp
ATGGTCTCCCCGGAAAACACCAACTGGCTCTTCGATTACGGGCTCATCGACGATACCCCTGTCCTGGATGGCAACTTCACTTGGCCCGTTCAGCCCATCGCCGGTTCGGCTAGCGTCAGATTGGGTCATGTGGTATGGTTGCTCATTTATTTTCTTTATTCTGTGGCATTCCCTAGTGTTGAACTTGATGGCTCATTGGGGGATGCTGAGGCACTTAAGGAATCTGGCTCAAAAAAGAGGGTCAGATCTGAATCATGCAGTGGAACAAGCTCGAAGGCATGTAGGGAGAAGTTGCGAAGGGAAAGGCTAAATGACAAGTTTGTGGAATTGGGCTCCATTTTGGAGCCTGGAAGGCCCCCCAAGACGGACAAGGCTGCCATTCTGGTTGATGCTGTACGAATGGTGAATCAATTACGTGGTGAAGCCCAGAAGTTGAAGGACACGAATTCAGGCCTCCAGGAGAAGATCAAGGAATTGAAGACAGAGAAGAATGAACTTCGTGATGAGAAGCAGAGGCTGAAATCAGAGAAAGAGAAGTTGGAACAGCAACTGAAATCCATGAATGCGCAGCCTGGCTTTTTGCCTCCCCCTCCTGGAATTCCTGCTGCATTTGCGGCTCAAGGCCAAGCTCACGGCAACAAGTTGGTGCCTTTCATTGGTTACCCCGGGGTTGCCATGTGGCAATTCATGCCACCTGCCGCTGTGGATACTTCGCAGGATCATGTACTCCGCCCACCGGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

249

Amino Acids

27.39

Weight (kDa)

8.43

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 703
Acc16I TGCGCA 1 cut(s) 567
Acc36I ACCTGC 1 cut(s) 703
AccB1I GGYRCC 1 cut(s) 646
AciI CCGC 3 cut(s) 614, 699, 730
AclWI GGATC 1 cut(s) 726
AcsI RAATTY 2 cut(s) 445, 597
AfaI GTAC 2 cut(s) 396, 726
AfiI CCNNNNNNNGG 6 cut(s) 64, 101, 364, 593, 656, 736
AflII CTTAAG 1 cut(s) 215
AgeI ACCGGT 1 cut(s) 736
AgsI TTSAA 3 cut(s) 182, 436, 478
AjnI CCWGG 5 cut(s) 63, 346, 456, 571, 592
AjuI GAANNNNNNNTTGG 4 cut(s) 14, 46, 521, 553
AluBI AGCT 2 cut(s) 270, 629
AluI AGCT 2 cut(s) 270, 629
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 1 cut(s) 726
AlwNI CAGNNNCTG 2 cut(s) 517, 550
Ama87I CYCGRG 1 cut(s) 665
AoxI GGCC 4 cut(s) 83, 353, 451, 622
ApeKI GCWGC 3 cut(s) 374, 568, 605
ApoI RAATTY 2 cut(s) 445, 597
AsiGI ACCGGT 1 cut(s) 736
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 2 cut(s) 84, 354
AsuC2I CCSGG 3 cut(s) 11, 666, 667
AsuHPI GGTGA 2 cut(s) 415, 431
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 665
BaeI ACNNNNGTAYC 2 cut(s) 45, 78
BanI GGYRCC 1 cut(s) 646
BanII GRGCYC 2 cut(s) 45, 335
BbsI GAAGAC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 3 cut(s) 361, 580, 592
BccI CCATC 3 cut(s) 62, 104, 182
BceAI ACGGC 1 cut(s) 649
BciT130I CCWGG 5 cut(s) 65, 348, 458, 573, 594
BciVI GTATCC 1 cut(s) 700
BcnI CCSGG 3 cut(s) 11, 666, 667
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 2 cut(s) 111, 174
BfrI CTTAAG 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 703
BfuI GTATCC 1 cut(s) 700
BglI GCCNNNNNGGC 2 cut(s) 107, 352
BglII AGATCT 1 cut(s) 245
BisI GCNGC 5 cut(s) 375, 569, 606, 615, 699
BlsI GCNGC 5 cut(s) 376, 570, 607, 616, 700
Bme1390I CCNGG 8 cut(s) 11, 65, 348, 458, 573, 594, 666, 667
BmeT110I CYCGRG 1 cut(s) 665
BmgT120I GGNCC 2 cut(s) 84, 354
BmiI GGNNCC 4 cut(s) 334, 345, 356, 648
BmrFI CCNGG 8 cut(s) 11, 65, 348, 458, 573, 594, 666, 667
BmsI GCATC 2 cut(s) 193, 379
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 440
Bpu10I CCTNAGC 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 603
BpuMI CCSGG 3 cut(s) 11, 666, 667
BsaAI YACGTR 1 cut(s) 416
BsaBI GATNNNNATC 1 cut(s) 250
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 4 cut(s) 9, 664, 665, 666
BsaWI WCCGGW 1 cut(s) 736
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 101, 364, 593, 656, 736
Bse118I RCCGGY 2 cut(s) 101, 736
Bse1I ACTGG 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 250
BseBI CCWGG 5 cut(s) 65, 348, 458, 573, 594
BseDI CCNNGG 4 cut(s) 9, 664, 665, 666
BseGI GGATG 2 cut(s) 73, 208
BseJI GATNNNNATC 1 cut(s) 250
BseLI CCNNNNNNNGG 6 cut(s) 64, 101, 364, 593, 656, 736
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 361, 580, 592
BshFI GGCC 4 cut(s) 85, 355, 453, 624
BshNI GGYRCC 1 cut(s) 646
BshTI ACCGGT 1 cut(s) 736
BsiHKCI CYCGRG 1 cut(s) 665
BsiSI CCGG 4 cut(s) 11, 102, 666, 737
BslI CCNNNNNNNGG 6 cut(s) 64, 101, 364, 593, 656, 736
BsmAI GTCTC 1 cut(s) 10
BsmI GAATGC 2 cut(s) 167, 568
BsnI GGCC 4 cut(s) 85, 355, 453, 624
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 1 cut(s) 665
Bsp1286I GDGCHC 2 cut(s) 45, 335
Bsp143I GATC 3 cut(s) 245, 465, 718
BspACI CCGC 3 cut(s) 614, 699, 730
BspANI GGCC 4 cut(s) 85, 355, 453, 624
BspCNI CTCAG 1 cut(s) 199
BspLI GGNNCC 4 cut(s) 334, 345, 356, 648
BspMI ACCTGC 1 cut(s) 703
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 726
BspQI GCTCTTC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 646
BspTI CTTAAG 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 10
BsrFI RCCGGY 2 cut(s) 101, 736
BsrI ACTGG 1 cut(s) 29
BssAI RCCGGY 2 cut(s) 101, 736
BssECI CCNNGG 4 cut(s) 9, 664, 665, 666
BssMI GATC 3 cut(s) 245, 465, 718
Bst2UI CCWGG 5 cut(s) 65, 348, 458, 573, 594
Bst6I CTCTTC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 215
BstAPI GCANNNNNTGC 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 1 cut(s) 207
BstEII GGTNACC 1 cut(s) 659
BstF5I GGATG 2 cut(s) 73, 208
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 3 cut(s) 248, 468, 721
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 3 cut(s) 245, 465, 718
BstMWI GCNNNNNNNGC 6 cut(s) 91, 107, 352, 611, 614, 688
BstNI CCWGG 5 cut(s) 65, 348, 458, 573, 594
BstNSI RCATGY 1 cut(s) 282
BstPI GGTNACC 1 cut(s) 659
BstSCI CCNGG 8 cut(s) 9, 63, 346, 456, 571, 592, 664, 665
BstV1I GCAGC 3 cut(s) 361, 580, 592
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BsuI GTATCC 1 cut(s) 700
BsuRI GGCC 4 cut(s) 85, 355, 453, 624
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 2 cut(s) 73, 208
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 265
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 1 cut(s) 112
CaiI CAGNNNCTG 2 cut(s) 517, 550
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 2 cut(s) 101, 736
Cfr13I GGNCC 2 cut(s) 84, 354
Cfr9I CCCGGG 1 cut(s) 665
CseI GACGC 1 cut(s) 103
Csp6I GTAC 2 cut(s) 395, 725
CspAI ACCGGT 1 cut(s) 736
CviAII CATG 7 cut(s) 128, 255, 279, 559, 676, 688, 722
CviQI GTAC 2 cut(s) 395, 725
DdeI CTNAG 1 cut(s) 207
DpnI GATC 3 cut(s) 247, 467, 720
DpnII GATC 3 cut(s) 245, 465, 718
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 1 cut(s) 719
Eco147I AGGCCT 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 45, 335
Eco31I GGTCTC 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 665
Eco91I GGTNACC 1 cut(s) 659
EcoO109I RGGNCCY 1 cut(s) 354
EcoO65I GGTNACC 1 cut(s) 659
EcoRI GAATTC 2 cut(s) 445, 597
EcoRII CCWGG 5 cut(s) 63, 346, 456, 571, 592
EcoT38I GRGCYC 2 cut(s) 45, 335
FaeI CATG 7 cut(s) 131, 258, 282, 562, 679, 691, 725
FaiI YATR 8 cut(s) 129, 136, 256, 280, 560, 677, 689, 723
FatI CATG 7 cut(s) 127, 254, 278, 558, 675, 687, 721
Fnu4HI GCNGC 5 cut(s) 375, 569, 606, 615, 699
FokI GGATG 2 cut(s) 80, 215
FriOI GRGCYC 2 cut(s) 45, 335
Fsp4HI GCNGC 5 cut(s) 375, 569, 606, 615, 699
FspBI CTAG 2 cut(s) 111, 174
FspI TGCGCA 1 cut(s) 567
GlaI GCGC 1 cut(s) 567
GluI GCNGC 5 cut(s) 375, 569, 606, 615, 699
GsuI CTGGAG 1 cut(s) 440
HaeIII GGCC 4 cut(s) 85, 355, 453, 624
HapII CCGG 4 cut(s) 11, 102, 666, 737
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 568
Hin1II CATG 7 cut(s) 131, 258, 282, 562, 679, 691, 725
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HinfI GANTC 3 cut(s) 221, 251, 406
HpaII CCGG 4 cut(s) 11, 102, 666, 737
HphI GGTGA 2 cut(s) 415, 431
Hpy188I TCNGA 4 cut(s) 119, 245, 250, 526
Hpy188III TCNNGA 1 cut(s) 500
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 268, 291, 345, 430
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 2 cut(s) 258, 608
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 107, 352, 611, 614, 688
HpyF3I CTNAG 1 cut(s) 207
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 7 cut(s) 131, 258, 282, 562, 679, 691, 725
HspAI GCGC 1 cut(s) 566
Kzo9I GATC 3 cut(s) 245, 465, 718
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 2 cut(s) 338, 343
Lsp1109I GCAGC 3 cut(s) 361, 580, 592
LweI GCATC 2 cut(s) 193, 379
MaeI CTAG 2 cut(s) 111, 174
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 1 cut(s) 659
MalI GATC 3 cut(s) 247, 467, 720
MboI GATC 3 cut(s) 245, 465, 718
MboII GAAGA 4 cut(s) 22, 475, 490, 499
MflI RGATCY 1 cut(s) 245
MhlI GDGCHC 2 cut(s) 45, 335
MluCI AATT 6 cut(s) 326, 410, 445, 473, 597, 683
MmeI TCCRAC 1 cut(s) 519
MnlI CCTC 6 cut(s) 202, 231, 464, 507, 594, 600
MseI TTAA 2 cut(s) 216, 745
MspA1I CMGCKG 1 cut(s) 701
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 4 cut(s) 11, 102, 666, 737
MspR9I CCNGG 8 cut(s) 11, 65, 348, 458, 573, 594, 666, 667
Mva1269I GAATGC 2 cut(s) 167, 568
MvaI CCWGG 5 cut(s) 65, 348, 458, 573, 594
MwoI GCNNNNNNNGC 6 cut(s) 91, 107, 352, 611, 614, 688
NciI CCSGG 3 cut(s) 11, 666, 667
NdeII GATC 3 cut(s) 245, 465, 718
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 7 cut(s) 131, 258, 282, 562, 679, 691, 725
NlaIV GGNNCC 4 cut(s) 334, 345, 356, 648
NsbI TGCGCA 1 cut(s) 567
NspI RCATGY 1 cut(s) 282
PaqCI CACCTGC 1 cut(s) 703
PceI AGGCCT 1 cut(s) 453
PciSI GCTCTTC 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 2 cut(s) 167, 568
PfeI GAWTC 3 cut(s) 221, 251, 406
PfoI TCCNGGA 3 cut(s) 63, 456, 592
PinAI ACCGGT 1 cut(s) 736
PkrI GCNGC 5 cut(s) 376, 570, 607, 616, 700
Ppu21I YACGTR 1 cut(s) 416
Psp6I CCWGG 5 cut(s) 63, 346, 456, 571, 592
PspEI GGTNACC 1 cut(s) 659
PspGI CCWGG 5 cut(s) 63, 346, 456, 571, 592
PspN4I GGNNCC 4 cut(s) 334, 345, 356, 648
PspPI GGNCC 2 cut(s) 84, 354
PstNI CAGNNNCTG 2 cut(s) 517, 550
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 2 cut(s) 396, 726
RsaNI GTAC 2 cut(s) 395, 725
SapI GCTCTTC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 216, 745
SatI GCNGC 5 cut(s) 375, 569, 606, 615, 699
Sau3AI GATC 3 cut(s) 245, 465, 718
Sau96I GGNCC 2 cut(s) 84, 354
ScrFI CCNGG 8 cut(s) 11, 65, 348, 458, 573, 594, 666, 667
SduI GDGCHC 2 cut(s) 45, 335
SetI ASST 4 cut(s) 272, 418, 631, 697
SfaNI GCATC 2 cut(s) 193, 379
SmaI CCCGGG 1 cut(s) 667
SmlI CTYRAG 2 cut(s) 215, 618
SmoI CTYRAG 2 cut(s) 215, 618
Sse9I AATT 6 cut(s) 326, 410, 445, 473, 597, 683
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 3 cut(s) 614, 699, 730
SspMI CTAG 2 cut(s) 111, 174
StuI AGGCCT 1 cut(s) 453
StyD4I CCNGG 8 cut(s) 9, 63, 346, 456, 571, 592, 664, 665
TaiI ACGT 1 cut(s) 418
TaqI TCGA 3 cut(s) 33, 48, 272
TasI AATT 6 cut(s) 326, 410, 445, 473, 597, 683
TatI WGTACW 1 cut(s) 724
TauI GCSGC 2 cut(s) 617, 701
TfiI GAWTC 3 cut(s) 221, 251, 406
Tru1I TTAA 2 cut(s) 216, 745
Tru9I TTAA 2 cut(s) 216, 745
TscAI CASTG 1 cut(s) 265
TseI GCWGC 3 cut(s) 374, 568, 605
TspDTI ATGAA 4 cut(s) 507, 575, 643, 676
TspGWI ACGGA 1 cut(s) 380
TspMI CCCGGG 1 cut(s) 665
TspRI CASTG 1 cut(s) 265
Vha464I CTTAAG 1 cut(s) 215
XapI RAATTY 2 cut(s) 445, 597
XceI RCATGY 1 cut(s) 282
XmaI CCCGGG 1 cut(s) 665
XspI CTAG 2 cut(s) 111, 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.