AT5G65000

Nucleotide-sugar transporter family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004039
Physical Location & Seq
Reverse (-)
25964929 .. 25967449
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65000.1

Sequence Viewer

Length: 978 bp
ATGGCGACGGCTAACGGAGCAAAGAGTCCGTCGAGTATGGGCCCTAAGGTTTTGTTTTATTCCATATTGCTCACACTTCAGTACGGAGCCCAGCCTCTGATCTCCAAACGCTGCATCAGAAAGGATGTTATTGTAACTTCATCTGTTTTGACGTGCGAGATTGTTAAGGTCATATGTGCTCTGATTCTCATGGCAAGAAATGGTAGTTTGAAGGGATTAGCAAAAGAGTGGACGTTGATGGGATCCTTGACAGCATCAGGACTTCCTGCAGCCATATATGCACTGCAGAACAGTTTGCTGCAGATCTCATACAGGAGTCTTGATTCCTTGACATTTTCAATTCTGAATCAGACGAAAATCTTTTTCACAGCATTCTTTACTTTCATAATACTAAGGCAGAAGCAATCAATTCTACAAATAGGAGCCTTGTGTCTATTGATCATGGCAGCAGTCCTTCTAAGTGTTGGTGAAGGCTCTAACAAAGATTCAAGCGGCATTAATGCGGATCAAAAGCTGTTTTATGGAATTATCCCGGTCTTGGCAGCCTCTGTCCTGTCGGGTTTAGCCTCTTCTCTGTGTCAATGGGCTTCTCAGGTCAAGAAGCATTCATCATACTTAATGACGGTTGAAATGTCTATCGTTGGAAGCCTCTGTTTATTAGTAAGTACTCTTAAATCTCCAGATGGTGAAGCGATTAAAAAATATGGCTTCTTTCATGGTTGGACTGCTTTAACACTGGTCCCAGTAATAAGCAATGCTCTTGGTGGGATTCTTGTTGGCCTAGTTACATCACATGCCGGTGGTGTAAGAAAGGGATTTGTGATTGTGTCGGCATTACTTGTGACGGCGCTACTTCAATTTGCGTTTGAAGGAAAACCGCCATCATCGTATTGCCTAGTTGCTCTTCCTCTTGTGATGAGTAGTATCTCAATGTACCAGAAATACCCATACATTGACAAGAAGAAGAAGAAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005338 GO:0005342 GO:0005463 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0006811 GO:0006820 GO:0007275 GO:0008150 GO:0008509 GO:0008514 GO:0009555 GO:0009653 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010208 GO:0010584 GO:0010605 GO:0010927 GO:0012505 GO:0015075 GO:0015136 GO:0015165 GO:0015318 GO:0015711 GO:0015739 GO:0015780 GO:0015789 GO:0015849 GO:0015931 GO:0015932 GO:0016043 GO:0019222 GO:0022607 GO:0022857 GO:0030162 GO:0030198 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032989 GO:0034220 GO:0042176 GO:0042177 GO:0043062 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045861 GO:0046942 GO:0046943 GO:0048229 GO:0048509 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051246 GO:0051248 GO:0051341 GO:0051354 GO:0055085 GO:0060255 GO:0061136 GO:0065007 GO:0065009 GO:0071702 GO:0071705 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0085029 GO:0090481 GO:0098656 GO:1901264 GO:1901505 GO:1901799 GO:1902183 GO:1903050 GO:1903051 GO:1903069 GO:1903070 GO:1903362 GO:1903363 GO:1903573 GO:1903825 GO:1903856 GO:1903857 GO:1904292 GO:1904293 GO:1905039 GO:1905897 GO:1990569 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nuc_sug_transp

Protein Analysis

325

Amino Acids

34.84

Weight (kDa)

9.67

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nuc_sug_transp PF04142 40 - 312 3e-31 Nucleotide-sugar transporter
PUNUT PF16913 91 - 311 3.7e-11 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 492, 503, 878
AclWI GGATC 3 cut(s) 237, 250, 513
AcuI CTGAAG 1 cut(s) 62
AfaI GTAC 3 cut(s) 83, 667, 935
AfiI CCNNNNNNNGG 1 cut(s) 538
AgsI TTSAA 6 cut(s) 211, 339, 489, 629, 857, 869
AjiI CACGTC 1 cut(s) 153
AluBI AGCT 1 cut(s) 514
AluI AGCT 1 cut(s) 514
Alw21I GWGCWC 1 cut(s) 181
AlwI GGATC 3 cut(s) 237, 250, 513
AlwNI CAGNNNCTG 2 cut(s) 97, 548
AoxI GGCC 2 cut(s) 40, 778
ApaI GGGCCC 1 cut(s) 44
ApeKI GCWGC 5 cut(s) 111, 269, 298, 446, 542
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AseI ATTAAT 1 cut(s) 498
AspLEI GCGC 1 cut(s) 850
AspS9I GGNCC 3 cut(s) 40, 41, 739
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 2 cut(s) 479, 698
AvaII GGWCC 1 cut(s) 739
AxyI CCTNAGG 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 44
BamHI GGATCC 1 cut(s) 242
BanII GRGCYC 2 cut(s) 44, 91
Bbv12I GWGCWC 1 cut(s) 181
BbvI GCAGC 5 cut(s) 98, 281, 285, 458, 554
BccI CCATC 3 cut(s) 232, 677, 889
BceAI ACGGC 2 cut(s) 24, 861
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 1 cut(s) 533
BfaI CTAG 2 cut(s) 782, 896
BfmI CTRYAG 3 cut(s) 267, 284, 299
BfoI RGCGCY 1 cut(s) 851
BglII AGATCT 1 cut(s) 303
BisI GCNGC 6 cut(s) 112, 270, 299, 447, 493, 543
BlsI GCNGC 6 cut(s) 113, 271, 300, 448, 494, 544
BmcAI AGTACT 1 cut(s) 667
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 1 cut(s) 739
BmgBI CACGTC 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 40, 41, 739
BmiI GGNNCC 5 cut(s) 42, 88, 244, 424, 741
BmrFI CCNGG 1 cut(s) 533
BmrI ACTGGG 1 cut(s) 737
BmsI GCATC 2 cut(s) 123, 263
BmuI ACTGGG 1 cut(s) 737
BpmI CTGGAG 1 cut(s) 663
BpuMI CCSGG 1 cut(s) 533
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 2 cut(s) 741, 743
Bse21I CCTNAGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 760
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 1 cut(s) 760
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 2 cut(s) 741, 743
BseSI GKGCMC 1 cut(s) 44
BseXI GCAGC 5 cut(s) 98, 281, 285, 458, 554
BseYI CCCAGC 1 cut(s) 90
BshFI GGCC 2 cut(s) 42, 780
BsiHKAI GWGCWC 1 cut(s) 181
BsiSI CCGG 2 cut(s) 533, 798
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 1 cut(s) 538
BsmFI GGGAC 1 cut(s) 725
BsmI GAATGC 2 cut(s) 371, 604
BsnI GGCC 2 cut(s) 42, 780
Bsp120I GGGCCC 1 cut(s) 40
Bsp1286I GDGCHC 3 cut(s) 44, 91, 181
Bsp143I GATC 5 cut(s) 99, 242, 303, 438, 505
BspACI CCGC 3 cut(s) 492, 503, 878
BspANI GGCC 2 cut(s) 42, 780
BspCNI CTCAG 1 cut(s) 604
BspLI GGNNCC 5 cut(s) 42, 88, 244, 424, 741
BspMAI CTGCAG 3 cut(s) 271, 288, 303
BspPI GGATC 3 cut(s) 237, 250, 513
BspQI GCTCTTC 1 cut(s) 909
BsrDI GCAATG 1 cut(s) 760
BsrFI RCCGGY 1 cut(s) 797
BsrI ACTGG 2 cut(s) 741, 743
BssAI RCCGGY 1 cut(s) 797
BssMI GATC 5 cut(s) 99, 242, 303, 438, 505
Bst4CI ACNGT 2 cut(s) 293, 625
Bst6I CTCTTC 2 cut(s) 574, 909
BstDEI CTNAG 4 cut(s) 45, 392, 458, 591
BstF5I GGATG 1 cut(s) 130
BstH2I RGCGCY 1 cut(s) 851
BstHHI GCGC 1 cut(s) 850
BstKTI GATC 5 cut(s) 102, 245, 306, 441, 508
BstMBI GATC 5 cut(s) 99, 242, 303, 438, 505
BstMWI GCNNNNNNNGC 2 cut(s) 17, 278
BstNSI RCATGY 1 cut(s) 797
BstSCI CCNGG 1 cut(s) 531
BstSFI CTRYAG 3 cut(s) 267, 284, 299
BstSLI GKGCMC 1 cut(s) 44
BstV1I GCAGC 5 cut(s) 98, 281, 285, 458, 554
BstX2I RGATCY 2 cut(s) 242, 303
BstYI RGATCY 2 cut(s) 242, 303
Bsu36I CCTNAGG 1 cut(s) 45
BsuRI GGCC 2 cut(s) 42, 780
BtrI CACGTC 1 cut(s) 153
BtsCI GGATG 1 cut(s) 130
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 2 cut(s) 281, 734
CaiI CAGNNNCTG 2 cut(s) 97, 548
CfoI GCGC 1 cut(s) 850
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 3 cut(s) 40, 41, 739
Csp6I GTAC 3 cut(s) 82, 666, 934
CviAII CATG 4 cut(s) 190, 442, 716, 794
CviQI GTAC 3 cut(s) 82, 666, 934
DdeI CTNAG 4 cut(s) 45, 392, 458, 591
DpnI GATC 5 cut(s) 101, 244, 305, 440, 507
DpnII GATC 5 cut(s) 99, 242, 303, 438, 505
Eam1104I CTCTTC 2 cut(s) 574, 909
EarI CTCTTC 2 cut(s) 574, 909
Eco24I GRGCYC 2 cut(s) 44, 91
Eco47I GGWCC 1 cut(s) 739
Eco57I CTGAAG 1 cut(s) 62
Eco81I CCTNAGG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 41
EcoT38I GRGCYC 2 cut(s) 44, 91
FaeI CATG 4 cut(s) 193, 445, 719, 797
FaqI GGGAC 1 cut(s) 725
FatI CATG 4 cut(s) 189, 441, 715, 793
FauNDI CATATG 1 cut(s) 173
FbaI TGATCA 1 cut(s) 438
Fnu4HI GCNGC 6 cut(s) 112, 270, 299, 447, 493, 543
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 2 cut(s) 44, 91
Fsp4HI GCNGC 6 cut(s) 112, 270, 299, 447, 493, 543
FspBI CTAG 2 cut(s) 782, 896
GlaI GCGC 1 cut(s) 849
GluI GCNGC 6 cut(s) 112, 270, 299, 447, 493, 543
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 1 cut(s) 663
HaeII RGCGCY 1 cut(s) 851
HaeIII GGCC 2 cut(s) 42, 780
HapII CCGG 2 cut(s) 533, 798
HhaI GCGC 1 cut(s) 850
Hin1II CATG 4 cut(s) 193, 445, 719, 797
Hin6I GCGC 1 cut(s) 848
HinP1I GCGC 1 cut(s) 848
HinfI GANTC 7 cut(s) 25, 184, 316, 323, 346, 485, 769
HpaII CCGG 2 cut(s) 533, 798
HphI GGTGA 2 cut(s) 479, 698
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 5 cut(s) 99, 119, 183, 345, 351
Hpy188III TCNNGA 4 cut(s) 258, 320, 598, 680
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 2 cut(s) 10, 34
HpyAV CCTTC 5 cut(s) 205, 464, 464, 863, 964
HpyCH4III ACNGT 2 cut(s) 293, 625
HpyCH4IV ACGT 2 cut(s) 152, 233
HpyCH4V TGCA 5 cut(s) 114, 269, 281, 286, 301
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 278
HpyF3I CTNAG 4 cut(s) 45, 392, 458, 591
HpySE526I ACGT 2 cut(s) 152, 233
Hsp92II CATG 4 cut(s) 193, 445, 719, 797
HspAI GCGC 1 cut(s) 848
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 5 cut(s) 99, 242, 303, 438, 505
LguI GCTCTTC 1 cut(s) 909
LmnI GCTCC 3 cut(s) 17, 86, 422
Lsp1109I GCAGC 5 cut(s) 98, 281, 285, 458, 554
LweI GCATC 2 cut(s) 123, 263
MaeI CTAG 2 cut(s) 782, 896
MaeII ACGT 2 cut(s) 152, 233
MaeIII GTNAC 3 cut(s) 133, 784, 841
MalI GATC 5 cut(s) 101, 244, 305, 440, 507
MboI GATC 5 cut(s) 99, 242, 303, 438, 505
MboII GAAGA 4 cut(s) 561, 896, 973, 976
MflI RGATCY 2 cut(s) 242, 303
MhlI GDGCHC 3 cut(s) 44, 91, 181
MluCI AATT 4 cut(s) 339, 408, 525, 857
MlyI GAGTC 2 cut(s) 34, 325
MmeI TCCRAC 2 cut(s) 622, 701
MnlI CCTC 5 cut(s) 105, 556, 577, 659, 918
MseI TTAA 6 cut(s) 165, 498, 617, 672, 696, 731
MslI CAYNNNNRTG 1 cut(s) 798
MspI CCGG 2 cut(s) 533, 798
MspR9I CCNGG 1 cut(s) 533
Mva1269I GAATGC 2 cut(s) 371, 604
MwoI GCNNNNNNNGC 2 cut(s) 17, 278
NciI CCSGG 1 cut(s) 533
NdeI CATATG 1 cut(s) 173
NdeII GATC 5 cut(s) 99, 242, 303, 438, 505
NlaIII CATG 4 cut(s) 193, 445, 719, 797
NlaIV GGNNCC 5 cut(s) 42, 88, 244, 424, 741
NmuCI GTSAC 1 cut(s) 841
NspI RCATGY 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 909
PctI GAATGC 2 cut(s) 371, 604
PfeI GAWTC 5 cut(s) 184, 323, 346, 485, 769
PkrI GCNGC 6 cut(s) 113, 271, 300, 448, 494, 544
PleI GAGTC 2 cut(s) 33, 324
PpsI GAGTC 2 cut(s) 33, 324
PshBI ATTAAT 1 cut(s) 498
PspFI CCCAGC 1 cut(s) 90
PspN4I GGNNCC 5 cut(s) 42, 88, 244, 424, 741
PspOMI GGGCCC 1 cut(s) 40
PspPI GGNCC 3 cut(s) 40, 41, 739
PstI CTGCAG 3 cut(s) 271, 288, 303
PstNI CAGNNNCTG 2 cut(s) 97, 548
PsuI RGATCY 2 cut(s) 242, 303
RsaI GTAC 3 cut(s) 83, 667, 935
RsaNI GTAC 3 cut(s) 82, 666, 934
RseI CAYNNNNRTG 1 cut(s) 798
SapI GCTCTTC 1 cut(s) 909
SaqAI TTAA 6 cut(s) 165, 498, 617, 672, 696, 731
SatI GCNGC 6 cut(s) 112, 270, 299, 447, 493, 543
Sau3AI GATC 5 cut(s) 99, 242, 303, 438, 505
Sau96I GGNCC 3 cut(s) 40, 41, 739
ScaI AGTACT 1 cut(s) 667
SchI GAGTC 2 cut(s) 34, 325
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 3 cut(s) 44, 91, 181
SetI ASST 7 cut(s) 51, 155, 171, 236, 516, 597, 975
SfaNI GCATC 2 cut(s) 123, 263
SfcI CTRYAG 3 cut(s) 267, 284, 299
SinI GGWCC 1 cut(s) 739
SmiMI CAYNNNNRTG 1 cut(s) 798
Sse9I AATT 4 cut(s) 339, 408, 525, 857
SsiI CCGC 3 cut(s) 492, 503, 878
SspMI CTAG 2 cut(s) 782, 896
StyD4I CCNGG 1 cut(s) 531
TaaI ACNGT 2 cut(s) 293, 625
TaiI ACGT 2 cut(s) 155, 236
TaqI TCGA 1 cut(s) 32
TasI AATT 4 cut(s) 339, 408, 525, 857
TatI WGTACW 1 cut(s) 665
TauI GCSGC 1 cut(s) 495
TfiI GAWTC 5 cut(s) 184, 323, 346, 485, 769
Tru1I TTAA 6 cut(s) 165, 498, 617, 672, 696, 731
Tru9I TTAA 6 cut(s) 165, 498, 617, 672, 696, 731
TscAI CASTG 2 cut(s) 288, 741
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 5 cut(s) 111, 269, 298, 446, 542
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 4 cut(s) 129, 373, 597, 704
TspGWI ACGGA 3 cut(s) 18, 30, 99
TspRI CASTG 2 cut(s) 288, 741
VpaK11BI GGWCC 1 cut(s) 739
VspI ATTAAT 1 cut(s) 498
XceI RCATGY 1 cut(s) 797
XspI CTAG 2 cut(s) 782, 896
ZrmI AGTACT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.