Rroxscaffold_2G00129190

CMP-sialic acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004039
Physical Location & Seq
Forward (+)
65030105 .. 65032876
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00129190.1

Sequence Viewer

Length: 987 bp
ATGGCGACCGCCGCCAATCCAAAACGTCCGCCGCCGACCTCGGACCAAATGAACGCCAAGGTCTTCCTCTACTCCCTTCTTCTGGCTCTCCAATACGGCGCTCAGCCTCTCATCTCCAAGCGCTTCATCAGAAGAGAAGTGATCGTAACGTCGTCTGTTCTGACGTGTGAGGTAGCCAAGGTCATTTGTGCCCTAATTTTCATGGCCAGAGACGGGAGCTTGAAGAAGGTCTACAAAGAGTGGACTTTGGTTGGTGCGTTGACCGCTTCGGGGCTTCCCGCTGCTATTTATGCACTGCAGAATAGTTTGCTTCAGATTTCGTACAAGAATCTCGATTCTTTAACCTTCTCAATGCTTAACCAGACTAAGATCATTTTTACTGCTATCTGTACTTATTTGATATTGAGGCAGAAGCAATCGATTCAACAAGTTGGGGCGCTTTTCTTGTTGATTATTGCAGCTGTTCTTTTAAGTTTCGGGGAAGGGTCGAGCAAAAGGTCTAGTGGTGGCAATTCTGACCAGATATTGTTTCATGGGATTATTCCTGTGTTGGTTGCGTCTGTGCTTTCGGGTTTGGCGTCCTCTCTGTGTCAATGGGCTTCCCAGGTTAAGAAGCACTCGTCGTACTTGATGACTGTAGAGATGTCTATTGTTGGAAGTTTGTGCATGTTGGTGAGTACCGCTAAATCTCCCGATGGAGAAGCTATCAGAAAACATGGACTTTTCTATGGATGGACTCTACTGACTTGGATCCCAGTCATGTCCAATGCCTTGGGTGGAATTCTTGTTGGCCTAGTCACAACCCATGCTGGCGGGGTTAGAAAGGGGTTTGTTATTGTTTCTGCGCTTCTTGTTACGGCTTTGCTGCAGTTCATTTTTGAAGGGAAACCACCTTCATTGTATTGTCTGGCATCTCTTCCCCTCGTTGTTAGCAGCATTTCGATATACCAGAAATATCCTTACCGAGTTAAAAAGAAAGAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005338 GO:0005342 GO:0005463 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0006811 GO:0006820 GO:0007275 GO:0008150 GO:0008509 GO:0008514 GO:0009555 GO:0009653 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010208 GO:0010584 GO:0010605 GO:0010927 GO:0012505 GO:0015075 GO:0015136 GO:0015165 GO:0015318 GO:0015711 GO:0015739 GO:0015780 GO:0015789 GO:0015849 GO:0015931 GO:0015932 GO:0016043 GO:0019222 GO:0022607 GO:0022857 GO:0030162 GO:0030198 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032434 GO:0032435 GO:0032501 GO:0032502 GO:0032989 GO:0034220 GO:0042176 GO:0042177 GO:0043062 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045861 GO:0046942 GO:0046943 GO:0048229 GO:0048509 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051246 GO:0051248 GO:0051341 GO:0051354 GO:0055085 GO:0060255 GO:0061136 GO:0065007 GO:0065009 GO:0071702 GO:0071705 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0085029 GO:0090481 GO:0098656 GO:1901264 GO:1901505 GO:1901799 GO:1902183 GO:1903050 GO:1903051 GO:1903069 GO:1903070 GO:1903362 GO:1903363 GO:1903573 GO:1903825 GO:1903856 GO:1903857 GO:1904292 GO:1904293 GO:1905039 GO:1905897 GO:1990569 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nuc_sug_transp

Protein Analysis

328

Amino Acids

35.56

Weight (kDa)

9.75

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 22 - 158 1.3e-07 EamA-like transporter family
Nuc_sug_transp PF04142 26 - 316 1.3e-32 Nucleotide-sugar transporter
PUNUT PF16913 95 - 222 7.7e-11 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 231
AciI CCGC 8 cut(s) 9, 12, 29, 32, 264, 279, 681, 813
AclWI GGATC 2 cut(s) 745, 758
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 780
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 578
AfaI GTAC 4 cut(s) 323, 391, 626, 679
AfeI AGCGCT 1 cut(s) 122
AfiI CCNNNNNNNGG 3 cut(s) 82, 213, 270
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 3 cut(s) 223, 425, 881
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 3 cut(s) 219, 461, 704
AluI AGCT 3 cut(s) 219, 461, 704
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 2 cut(s) 745, 758
Aor51HI AGCGCT 1 cut(s) 122
AoxI GGCC 2 cut(s) 204, 790
ApeKI GCWGC 4 cut(s) 281, 458, 865, 933
ApoI RAATTY 1 cut(s) 780
AspLEI GCGC 4 cut(s) 101, 123, 439, 847
AspS9I GGNCC 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 685
AvaII GGWCC 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 193
BalI TGGCCA 1 cut(s) 206
BamHI GGATCC 1 cut(s) 750
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BbsI GAAGAC 1 cut(s) 55
BbvI GCAGC 4 cut(s) 268, 470, 852, 945
BccI CCATC 2 cut(s) 689, 726
BceAI ACGGC 2 cut(s) 112, 873
BciT130I CCWGG 1 cut(s) 605
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 2 cut(s) 501, 794
BfmI CTRYAG 3 cut(s) 296, 636, 866
BfoI RGCGCY 3 cut(s) 102, 124, 440
BisI GCNGC 6 cut(s) 12, 32, 282, 459, 866, 934
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 6 cut(s) 13, 33, 283, 460, 867, 935
Bme1390I CCNGG 1 cut(s) 605
Bme18I GGWCC 1 cut(s) 43
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 1 cut(s) 752
BmrFI CCNGG 1 cut(s) 605
BmrI ACTGGG 1 cut(s) 749
BmsI GCATC 1 cut(s) 920
BmuI ACTGGG 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 55
Bpu1102I GCTNAGC 1 cut(s) 102
Bsa29I ATCGAT 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 578
BsaJI CCNNGG 5 cut(s) 39, 57, 177, 603, 771
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 213, 270
Bse1I ACTGG 1 cut(s) 755
BseBI CCWGG 1 cut(s) 605
BseCI ATCGAT 1 cut(s) 419
BseDI CCNNGG 5 cut(s) 39, 57, 177, 603, 771
BseGI GGATG 1 cut(s) 737
BseLI CCNNNNNNNGG 3 cut(s) 82, 213, 270
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 755
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 4 cut(s) 268, 470, 852, 945
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 206, 792
BshVI ATCGAT 1 cut(s) 419
BsiEI CGRYCG 1 cut(s) 9
BslI CCNNNNNNNGG 3 cut(s) 82, 213, 270
BsmAI GTCTC 1 cut(s) 204
BsmBI CGTCTC 1 cut(s) 204
BsnI GGCC 2 cut(s) 206, 792
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 3 cut(s) 141, 369, 750
Bsp1720I GCTNAGC 1 cut(s) 102
BspACI CCGC 8 cut(s) 9, 12, 29, 32, 264, 279, 681, 813
BspANI GGCC 2 cut(s) 206, 792
BspCNI CTCAG 1 cut(s) 115
BspDI ATCGAT 1 cut(s) 419
BspLI GGNNCC 1 cut(s) 752
BspMAI CTGCAG 2 cut(s) 300, 870
BspPI GGATC 2 cut(s) 745, 758
BsrI ACTGG 1 cut(s) 755
BssECI CCNNGG 5 cut(s) 39, 57, 177, 603, 771
BssMI GATC 3 cut(s) 141, 369, 750
BssNI GRCGYC 1 cut(s) 578
BssT1I CCWWGG 3 cut(s) 57, 177, 771
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 637
Bst6I CTCTTC 2 cut(s) 127, 921
BstACI GRCGYC 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 811
BstDEI CTNAG 2 cut(s) 102, 366
BstF5I GGATG 1 cut(s) 737
BstH2I RGCGCY 3 cut(s) 102, 124, 440
BstHHI GCGC 4 cut(s) 101, 123, 439, 847
BstKTI GATC 3 cut(s) 144, 372, 753
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 3 cut(s) 141, 369, 750
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 11, 263, 290
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 1 cut(s) 603
BstSFI CTRYAG 3 cut(s) 296, 636, 866
BstSLI GKGCMC 1 cut(s) 193
BstV1I GCAGC 4 cut(s) 268, 470, 852, 945
BstV2I GAAGAC 1 cut(s) 55
BstX2I RGATCY 1 cut(s) 750
BstXI CCANNNNNNTGG 1 cut(s) 772
BstYI RGATCY 1 cut(s) 750
Bsu15I ATCGAT 1 cut(s) 419
BsuRI GGCC 2 cut(s) 206, 792
BsuTUI ATCGAT 1 cut(s) 419
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 737
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 811
CfoI GCGC 4 cut(s) 101, 123, 439, 847
Cfr13I GGNCC 1 cut(s) 43
ClaI ATCGAT 1 cut(s) 419
CseI GACGC 2 cut(s) 546, 567
Csp6I GTAC 4 cut(s) 322, 390, 625, 678
CspCI CAANNNNNGTGG 2 cut(s) 879, 914
CviAII CATG 6 cut(s) 202, 533, 667, 716, 760, 806
CviQI GTAC 4 cut(s) 322, 390, 625, 678
DdeI CTNAG 2 cut(s) 102, 366
DpnI GATC 3 cut(s) 143, 371, 752
DpnII GATC 3 cut(s) 141, 369, 750
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 2 cut(s) 127, 921
EarI CTCTTC 2 cut(s) 127, 921
EciI GGCGGA 1 cut(s) 18
Eco130I CCWWGG 3 cut(s) 57, 177, 771
Eco47I GGWCC 1 cut(s) 43
Eco47III AGCGCT 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 780
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 3 cut(s) 57, 177, 771
ErhI CCWWGG 3 cut(s) 57, 177, 771
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 6 cut(s) 205, 536, 670, 719, 763, 809
FaiI YATR 9 cut(s) 203, 291, 534, 668, 717, 729, 761, 807, 946
FatI CATG 6 cut(s) 201, 532, 666, 715, 759, 805
FauI CCCGC 2 cut(s) 286, 806
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 6 cut(s) 12, 32, 282, 459, 866, 934
FokI GGATG 1 cut(s) 744
Fsp4HI GCNGC 6 cut(s) 12, 32, 282, 459, 866, 934
FspBI CTAG 2 cut(s) 501, 794
GlaI GCGC 4 cut(s) 100, 122, 438, 846
GluI GCNGC 6 cut(s) 12, 32, 282, 459, 866, 934
HaeII RGCGCY 3 cut(s) 102, 124, 440
HaeIII GGCC 2 cut(s) 206, 792
HgaI GACGC 2 cut(s) 546, 567
HhaI GCGC 4 cut(s) 101, 123, 439, 847
Hin1I GRCGYC 1 cut(s) 578
Hin1II CATG 6 cut(s) 205, 536, 670, 719, 763, 809
Hin6I GCGC 4 cut(s) 99, 121, 437, 845
HinP1I GCGC 4 cut(s) 99, 121, 437, 845
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 5 cut(s) 328, 335, 421, 736, 980
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 3 cut(s) 232, 243, 261
Hpy188I TCNGA 6 cut(s) 43, 131, 162, 315, 517, 710
Hpy188III TCNNGA 2 cut(s) 332, 692
Hpy8I GTNNAC 3 cut(s) 232, 243, 261
Hpy99I CGWCG 2 cut(s) 154, 625
HpyAV CCTTC 6 cut(s) 86, 220, 355, 476, 875, 903
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4IV ACGT 3 cut(s) 25, 149, 164
HpyCH4V TGCA 5 cut(s) 293, 298, 458, 666, 868
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 263, 290
HpyF3I CTNAG 2 cut(s) 102, 366
HpySE526I ACGT 3 cut(s) 25, 149, 164
Hsp92I GRCGYC 1 cut(s) 578
Hsp92II CATG 6 cut(s) 205, 536, 670, 719, 763, 809
HspAI GCGC 4 cut(s) 99, 121, 437, 845
Kzo9I GATC 3 cut(s) 141, 369, 750
LmnI GCTCC 1 cut(s) 216
Lsp1109I GCAGC 4 cut(s) 268, 470, 852, 945
LweI GCATC 1 cut(s) 920
MaeI CTAG 2 cut(s) 501, 794
MaeII ACGT 3 cut(s) 25, 149, 164
MaeIII GTNAC 3 cut(s) 145, 796, 853
MalI GATC 3 cut(s) 143, 371, 752
MboI GATC 3 cut(s) 141, 369, 750
MboII GAAGA 5 cut(s) 55, 71, 144, 235, 908
MflI RGATCY 1 cut(s) 750
MhlI GDGCHC 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 3 cut(s) 195, 511, 780
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 1 cut(s) 730
MmeI TCCRAC 1 cut(s) 634
MnlI CCTC 7 cut(s) 49, 77, 117, 163, 399, 592, 932
Mox20I TGGCCA 1 cut(s) 206
MscI TGGCCA 1 cut(s) 206
MseI TTAA 6 cut(s) 341, 357, 470, 609, 969, 985
MslI CAYNNNNRTG 1 cut(s) 671
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 2 cut(s) 281, 461
MspR9I CCNGG 1 cut(s) 605
MvaI CCWGG 1 cut(s) 605
MwoI GCNNNNNNNGC 3 cut(s) 11, 263, 290
NdeII GATC 3 cut(s) 141, 369, 750
NlaIII CATG 6 cut(s) 205, 536, 670, 719, 763, 809
NlaIV GGNNCC 1 cut(s) 752
NmuCI GTSAC 1 cut(s) 796
NspI RCATGY 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 575
PfeI GAWTC 4 cut(s) 328, 335, 421, 980
PkrI GCNGC 6 cut(s) 13, 33, 283, 460, 867, 935
PleI GAGTC 1 cut(s) 730
PpsI GAGTC 1 cut(s) 730
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 752
PspPI GGNCC 1 cut(s) 43
PstI CTGCAG 2 cut(s) 300, 870
PsuI RGATCY 1 cut(s) 750
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 4 cut(s) 323, 391, 626, 679
RsaNI GTAC 4 cut(s) 322, 390, 625, 678
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 6 cut(s) 341, 357, 470, 609, 969, 985
SatI GCNGC 6 cut(s) 12, 32, 282, 459, 866, 934
Sau3AI GATC 3 cut(s) 141, 369, 750
Sau96I GGNCC 1 cut(s) 43
SchI GAGTC 1 cut(s) 730
ScrFI CCNGG 1 cut(s) 605
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 1 cut(s) 920
SfcI CTRYAG 3 cut(s) 296, 636, 866
SinI GGWCC 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 671
Sse9I AATT 3 cut(s) 195, 511, 780
SsiI CCGC 8 cut(s) 9, 12, 29, 32, 264, 279, 681, 813
SspMI CTAG 2 cut(s) 501, 794
StyD4I CCNGG 1 cut(s) 603
StyI CCWWGG 3 cut(s) 57, 177, 771
TaaI ACNGT 1 cut(s) 637
TaiI ACGT 3 cut(s) 28, 152, 167
TaqI TCGA 4 cut(s) 333, 419, 488, 941
TasI AATT 3 cut(s) 195, 511, 780
TatI WGTACW 1 cut(s) 389
TauI GCSGC 2 cut(s) 14, 34
TfiI GAWTC 4 cut(s) 328, 335, 421, 980
Tru1I TTAA 6 cut(s) 341, 357, 470, 609, 969, 985
Tru9I TTAA 6 cut(s) 341, 357, 470, 609, 969, 985
TscAI CASTG 1 cut(s) 300
TseFI GTSAC 1 cut(s) 796
TseI GCWGC 4 cut(s) 281, 458, 865, 933
Tsp45I GTSAC 1 cut(s) 796
TspDTI ATGAA 6 cut(s) 65, 115, 190, 521, 862, 885
TspRI CASTG 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 1 cut(s) 780
XceI RCATGY 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 2 cut(s) 501, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.