Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004392

Physical Location & Seq

Genomic Coordinates Forward (+)

26221829 .. 26225903

Gene Structure 4075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G65620.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGTTAATGGCGACTCCAACGTCTCGAGCCTCTCTCAATCTTCTTCGTCGATCGCCAAAACCCAAATATTTCTCATCTTCTTCTTGTCATTTTCGTCCCTCAACTTTTCGTAAATCATATCCTTGCCCCATCTGGTCTTCTTCTTTCTCTTTTTGTCTCCCTCCTCCTAGATCCACCACTTCCACCTCTCTCTCCTCCTCCTCTTTCCGCCCCTTCTCCTCTCCTCCGTCCATGTCTTCCGCCGCCGCTGCCGCCGTGGAATCCGTCGTCTCCGACGAGACTCTTTCCTCGAATCCTCTCTTGCAGGACTTCGATTTCCCTCCGTTTGATTCCGTTGATGCCAGTCATGTTCGTCCTGGGATTCGTGCTCTATTGCAGCATCTCGAAGCTGAATTGGAGGAGCTAGAGAAATCTGTGGAGCCAACATGGCCAAAACTGGTGGAACCATTAGAGAAAATCGTTGATCGGTTAACTGTTGTTTGGGGAATGATCAATCACCTTAAGGCTGTCAAGGACACACCTGAGCTACGTGCTGCCATCGAAGACGTTCAGCCAGAGAAAGTGAAGTTCCAGCTCAGGTTGGGGCAAAGCAAGCCTATTTACAATGCCTTTAAAGCTATTCGTGAATCTCCTGATTGGTCATCGCTCAGTGAAGCTCGTCAACGCTTAGTAGAAGCTCAAATAAAGGAGGCGGTTCTCATTGGTATTGCTCTTGATGATGAAAAGAGAGAAGAGTTTAATAAAATTGAACAGGAACTCGAAAAACTTTCCCATAAGTTTTCTGAGAATGTTTTGGATGCAACAAAGAAATTTGAAAAGTTGATAACAGACAAGAAAGAGATCGAGGGATTGCCTCCATCTGCTCTTGGGCTATTCGCACAAGCAGCTGTCTCCAAGGGCCATGAAAATGCAACTGCTGAGAATGGACCATGGATCATTACACTGGATGCTCCTAGTTATCTTCCTGTCATGCAACATGCTAAAAACCGTGCTCTGCGTGAGGAAGTCTACCGTGCTTACCTATCTCGTGCCTCTTCTGGCGATTTGGATAATACGGCAATTATTGACCAGATTTTGAAGCTTCGCTTGGAAAAAGCTAAGCTTCTTGGTTACAACAATTACGCTGAGGTAAGCATGGCTATGAAAATGGCTACTGTTGAGAAAGCAGCAGAGCTTTTAGAGAAGCTTCGTAGTGCATCCTGGGATGCGGCTGTTCAAGACATGGAAGACCTCAAGAGTTTCGCAAAGAACCAGGGTGCTGCAGAATCTGACAGTATGACTCATTGGGACACCACTTTCTGGAGTGAGAGGCTTCGTGAATCCAAATACGATATTAATGAGGAAGAACTACGTCCATACTTCTCACTGCCAAAGGTTATGGATGGACTTTTCAGTCTAGCTAAGACACTTTTTGGAATTGATATTGAACCAGCAGATGGTTTAGCTCCGGTCTGGAACAATGATGTTCGGTTCTACCGCGTCAAAGATTCTTCTGGGAACCCAATCGCTTACTTTTACTTTGATCCATACTCTCGCCCGTCAGAGAAAAGAGGTGGCGCTTGGATGGATGAGGTTGTTTCCCGTAGCCGAGTCATGGCTCAGAAAGGCTCTTCTGTTAGGCTGCCTGTTGCACACATGGTCTGCAACCAAACTCCACCAGTAGGTGACAAGCCAAGCCTTATGACATTCCGTGAGGTAGAGACAGTATTTCATGAATTTGGACATGCTCTTCAGCACATGCTTACGAAGCAGGATGAGGGGCTAGTTGCTGGTATCCGAAATATCGAGTGGGACGCGGTTGAATTACCATCACAGTTTATGGAGAATTGGTGCTACCACAGGGACACTTTAATGAGCATTGCTAAGCATTACGAAACAGGAGAAACACTCCCAGAGGAAGTATACAAGAAACTCCTGGCCGCAAGAACATTCCGTGCAGGGTCCTTTAGTCTTCGTCAGTTGAAATTTGCCAGTGTTGATCTTGAGCTGCACACAAAATACGTACCAGGAGGACCAGAGTCCATCTATGACGTAGATCAAAGGGTATCAGTAAAAACCCAAGTTATCCCTCCACTTCCTGAGGATAGATTTCTCTGTAGCTTCAGTCACATCTTTGCCGGGGGATATGCAGCTGGATATTACAGTTACAAGTGGGCAGAAGTTTTGTCTGCTGATGCATTTTCAGCATTTGAAGACGCTGGACTAGACGACATCAAGGCTGTTAAAGAAACAGGACAGAGATTCAGAAACACCATACTTGCACTTGGAGGAGGGAAAGCGCCTCTTAAGGTGTTTGTTGAGTTTAGAGGACGAGAACCTTCACCCGAGCCTCTTCTCAGACACAATGGACTCTTGGCTGCCTCTGCTTCTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031974 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M3

Protein Analysis

791

Amino Acids

88.76

Weight (kDa)

5.9

Isoelectric Point (pI)

50.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP_N	PF19310	135 - 257	1e-56	Neurolysin/Thimet oligopeptidase, N-terminal domain
Peptidase_M3	PF01432	337 - 797	9.2e-142	Peptidase family M3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10540 AT5G65620 AT5G65620
fragaria_vesca	FvH4_1g19050 FvH4_1g19050 FvH4_1g19050
malus_domestica	MD01G1044200.v1.1
prunus_persica	Prupe.6G167100_v2.0.a1 Prupe.6G167100_v2.0.a1 Prupe.6G209200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0110061 RchiOBHm_Chr7g0233391
rosa_laevigata	RLG00000001272 RLG00000017783
rosa_multiflora	Rmu_co8163522.1_g000001 Rmu_co8337137.1_g000001 Rmu_sc0008806.1_g000009 Rmu_sc0009935.1_g000003 Rmu_sc0013750.1_g000001 Rmu_sc0024311.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133750
rosa_rugosa	Rorug02G0165400 Rorug02G0165400
rosa_samantha	Rh2AG217200 Rh2BG227200 Rh2CG219400 Rh2DG222900 Rh7CG455700
rosa_wichuraiana	Rw0G010890 Rw2G016780 Rw7G035960 Rw7G035980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	19, 1392
AccB7I	CCANNNNNTGG	2 cut(s)	1299, 1436
AccI	GTMKAC	2 cut(s)	1008, 1902
AccII	CGCG	2 cut(s)	1479, 1796
AclWI	GGATC	3 cut(s)	165, 941, 1517
AcoI	YGGCCR	2 cut(s)	428, 1917
AcsI	RAATTY	3 cut(s)	809, 1715, 1964
AcuI	CTGAAG	2 cut(s)	1715, 2086
AfaI	GTAC	1 cut(s)	2004
AfiI	CCNNNNNNNGG	4 cut(s)	1299, 1436, 1594, 1661
AflII	CTTAAG	2 cut(s)	500, 2285
AgsI	TTSAA	8 cut(s)	749, 815, 1078, 1217, 1427, 1802, 1963, 2192
AjnI	CCWGG	5 cut(s)	355, 1199, 1251, 1914, 2005
AjuI	GAANNNNNNNTTGG	2 cut(s)	1070, 1102
Alw21I	GWGCWC	2 cut(s)	370, 994
Alw26I	GTCTC	6 cut(s)	27, 161, 272, 274, 895, 1694
AlwI	GGATC	3 cut(s)	165, 941, 1517
AlwNI	CAGNNNCTG	1 cut(s)	1268
Ama87I	CYCGRG	2 cut(s)	24, 2324
AoxI	GGCC	3 cut(s)	428, 898, 1917
ApoI	RAATTY	3 cut(s)	809, 1715, 1964
AseI	ATTAAT	1 cut(s)	1335
Asp700I	GAANNNNTTC	1 cut(s)	546
AspLEI	GCGC	2 cut(s)	1559, 2281
AspS9I	GGNCC	4 cut(s)	898, 926, 1941, 2012
AsuC2I	CCSGG	1 cut(s)	2119
AsuHPI	GGTGA	3 cut(s)	488, 1676, 2313
AvaI	CYCGRG	2 cut(s)	24, 2324
AvaII	GGWCC	3 cut(s)	926, 1941, 2012
AxyI	CCTNAGG	1 cut(s)	2079
BalI	TGGCCA	1 cut(s)	430
BauI	CACGAG	1 cut(s)	1026
BbsI	GAAGAC	6 cut(s)	129, 228, 549, 1233, 1943, 2199
Bbv12I	GWGCWC	2 cut(s)	370, 994
BbvCI	CCTCAGC	1 cut(s)	1125
BccI	CCATC	8 cut(s)	137, 545, 865, 1376, 1430, 1558, 1816, 2030
BceAI	ACGGC	2 cut(s)	239, 1071
BciT130I	CCWGG	5 cut(s)	357, 1201, 1253, 1916, 2007
BciVI	GTATCC	1 cut(s)	1784
BclI	TGATCA	1 cut(s)	489
BcnI	CCSGG	1 cut(s)	2119
BcoDI	GTCTC	6 cut(s)	27, 161, 272, 274, 895, 1694
BfaI	CTAG	6 cut(s)	168, 404, 954, 1397, 1763, 2204
BfmI	CTRYAG	2 cut(s)	1260, 2095
BfoI	RGCGCY	2 cut(s)	1560, 2282
BfrI	CTTAAG	2 cut(s)	500, 2285
BfuI	GTATCC	1 cut(s)	1784
BglI	GCCNNNNNGGC	1 cut(s)	427
BlpI	GCTNAGC	2 cut(s)	1098, 1863
Bme1390I	CCNGG	6 cut(s)	357, 1201, 1253, 1916, 2007, 2119
Bme18I	GGWCC	3 cut(s)	926, 1941, 2012
BmeT110I	CYCGRG	2 cut(s)	24, 2324
BmgT120I	GGNCC	4 cut(s)	898, 926, 1941, 2012
BmiI	GGNNCC	4 cut(s)	420, 444, 1499, 1942
BmrFI	CCNGG	6 cut(s)	357, 1201, 1253, 1916, 2007, 2119
BmsI	GCATC	7 cut(s)	328, 388, 787, 937, 1195, 1205, 2164
BoxI	GACNNNNGTC	1 cut(s)	2017
BpiI	GAAGAC	6 cut(s)	129, 228, 549, 1233, 1943, 2199
BplI	GAGNNNNNCTC	4 cut(s)	18, 50, 1872, 1904
BpmI	CTGGAG	1 cut(s)	1321
Bpu10I	CCTNAGC	3 cut(s)	522, 575, 1125
Bpu1102I	GCTNAGC	2 cut(s)	1098, 1863
BpuEI	CTTGAG	2 cut(s)	1217, 2003
BpuMI	CCSGG	1 cut(s)	2119
BsaAI	YACGTR	2 cut(s)	530, 2002
BsaJI	CCNNGG	7 cut(s)	255, 356, 894, 929, 1200, 1252, 2118
BsaWI	WCCGGW	1 cut(s)	1447
BsaXI	ACNNNNNCTCC	2 cut(s)	1814, 1844
Bsc4I	CCNNNNNNNGG	4 cut(s)	1299, 1436, 1594, 1661
Bse1I	ACTGG	5 cut(s)	342, 441, 948, 1658, 1971
Bse21I	CCTNAGG	1 cut(s)	2079
Bse3DI	GCAATG	1 cut(s)	1857
BseBI	CCWGG	5 cut(s)	357, 1201, 1253, 1916, 2007
BseDI	CCNNGG	7 cut(s)	255, 356, 894, 929, 1200, 1252, 2118
BseGI	GGATG	8 cut(s)	802, 952, 1196, 1210, 1387, 1569, 1573, 1759
BseLI	CCNNNNNNNGG	4 cut(s)	1299, 1436, 1594, 1661
BseMI	GCAATG	1 cut(s)	1857
BseMII	CTCAG	9 cut(s)	513, 589, 661, 774, 909, 1116, 1613, 2070, 2350
BseNI	ACTGG	5 cut(s)	342, 441, 948, 1658, 1971
BseRI	GAGGAG	8 cut(s)	153, 184, 187, 190, 208, 213, 413, 2283
BsgI	GTGCAG	2 cut(s)	1956, 1973
Bsh1236I	CGCG	2 cut(s)	1479, 1796
Bsh1285I	CGRYCG	1 cut(s)	53
BshFI	GGCC	3 cut(s)	430, 900, 1919
BsiEI	CGRYCG	1 cut(s)	53
BsiHKAI	GWGCWC	2 cut(s)	370, 994
BsiHKCI	CYCGRG	2 cut(s)	24, 2324
BsiSI	CCGG	2 cut(s)	1448, 2118
BslFI	GGGAC	4 cut(s)	81, 1301, 1805, 1856
BslI	CCNNNNNNNGG	4 cut(s)	1299, 1436, 1594, 1661
BsmAI	GTCTC	6 cut(s)	27, 161, 272, 274, 895, 1694
BsmBI	CGTCTC	2 cut(s)	27, 274
BsmFI	GGGAC	4 cut(s)	81, 1301, 1805, 1856
BsnI	GGCC	3 cut(s)	430, 900, 1919
BsoBI	CYCGRG	2 cut(s)	24, 2324
Bsp1286I	GDGCHC	2 cut(s)	370, 994
Bsp143I	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
Bsp1720I	GCTNAGC	2 cut(s)	1098, 1863
Bsp19I	CCATGG	1 cut(s)	929
BspANI	GGCC	3 cut(s)	430, 900, 1919
BspCNI	CTCAG	9 cut(s)	514, 588, 660, 775, 910, 1117, 1612, 2071, 2349
BspFNI	CGCG	2 cut(s)	1479, 1796
BspHI	TCATGA	1 cut(s)	1711
BspLI	GGNNCC	4 cut(s)	420, 444, 1499, 1942
BspMAI	CTGCAG	1 cut(s)	1264
BspPI	GGATC	3 cut(s)	165, 941, 1517
BspQI	GCTCTTC	2 cut(s)	1615, 1734
BspTI	CTTAAG	2 cut(s)	500, 2285
BsrDI	GCAATG	1 cut(s)	1857
BsrI	ACTGG	5 cut(s)	342, 441, 948, 1658, 1971
BssECI	CCNNGG	7 cut(s)	255, 356, 894, 929, 1200, 1252, 2118
BssMI	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
BssNAI	GTATAC	1 cut(s)	1903
BssSI	CACGAG	1 cut(s)	1026
BssT1I	CCWWGG	2 cut(s)	894, 929
Bst1107I	GTATAC	1 cut(s)	1903
Bst2BI	CACGAG	1 cut(s)	1026
Bst2UI	CCWGG	5 cut(s)	357, 1201, 1253, 1916, 2007
Bst4CI	ACNGT	8 cut(s)	475, 989, 1013, 1156, 1274, 1705, 1815, 2144
Bst6I	CTCTTC	5 cut(s)	726, 1039, 1615, 1734, 2337
BstAFI	CTTAAG	2 cut(s)	500, 2285
BstBAI	YACGTR	2 cut(s)	530, 2002
BstC8I	GCNNGC	1 cut(s)	593
BstDSI	CCRYGG	2 cut(s)	255, 929
BstF5I	GGATG	8 cut(s)	802, 952, 1196, 1210, 1387, 1569, 1573, 1759
BstFNI	CGCG	2 cut(s)	1479, 1796
BstH2I	RGCGCY	2 cut(s)	1560, 2282
BstHHI	GCGC	2 cut(s)	1559, 2281
BstKTI	GATC	9 cut(s)	53, 173, 466, 492, 843, 936, 1525, 1981, 2038
BstMAI	GTCTC	6 cut(s)	27, 161, 272, 274, 895, 1694
BstMBI	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
BstMCI	CGRYCG	1 cut(s)	53
BstMWI	GCNNNNNNNGC	9 cut(s)	248, 251, 427, 592, 614, 884, 1747, 2183, 2363
BstNI	CCWGG	5 cut(s)	357, 1201, 1253, 1916, 2007
BstNSI	RCATGY	3 cut(s)	980, 1727, 1741
BstPAI	GACNNNNGTC	1 cut(s)	2017
BstSCI	CCNGG	6 cut(s)	355, 1199, 1251, 1914, 2005, 2117
BstSFI	CTRYAG	2 cut(s)	1260, 2095
BstSNI	TACGTA	1 cut(s)	2002
BstUI	CGCG	2 cut(s)	1479, 1796
BstV2I	GAAGAC	6 cut(s)	129, 228, 549, 1233, 1943, 2199
BstX2I	RGATCY	1 cut(s)	170
BstYI	RGATCY	1 cut(s)	170
BstZ17I	GTATAC	1 cut(s)	1903
Bsu36I	CCTNAGG	1 cut(s)	2079
BsuI	GTATCC	1 cut(s)	1784
BsuRI	GGCC	3 cut(s)	430, 900, 1919
BtgI	CCRYGG	2 cut(s)	255, 929
BtgZI	GCGATG	1 cut(s)	627
BtsCI	GGATG	8 cut(s)	802, 952, 1196, 1210, 1387, 1569, 1573, 1759
BtsI	GCAGTG	1 cut(s)	1364
BtsIMutI	CAGTG	4 cut(s)	655, 941, 1364, 1978
Cac8I	GCNNGC	1 cut(s)	593
CaiI	CAGNNNCTG	1 cut(s)	1268
CciI	TCATGA	1 cut(s)	1711
CfoI	GCGC	2 cut(s)	1559, 2281
Cfr13I	GGNCC	4 cut(s)	898, 926, 1941, 2012
CseI	GACGC	3 cut(s)	1468, 1802, 2204
Csp6I	GTAC	1 cut(s)	2003
CspCI	CAANNNNNGTGG	2 cut(s)	420, 455
CviQI	GTAC	1 cut(s)	2003
DpnI	GATC	9 cut(s)	52, 172, 465, 491, 842, 935, 1524, 1980, 2037
DpnII	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
DraI	TTTAAA	1 cut(s)	613
DrdI	GACNNNNNNGTC	2 cut(s)	19, 1392
DseDI	GACNNNNNNGTC	2 cut(s)	19, 1392
EaeI	YGGCCR	2 cut(s)	428, 1917
Eam1104I	CTCTTC	5 cut(s)	726, 1039, 1615, 1734, 2337
EarI	CTCTTC	5 cut(s)	726, 1039, 1615, 1734, 2337
EciI	GGCGGA	2 cut(s)	197, 229
Eco105I	TACGTA	1 cut(s)	2002
Eco130I	CCWWGG	2 cut(s)	894, 929
Eco47I	GGWCC	3 cut(s)	926, 1941, 2012
Eco57I	CTGAAG	2 cut(s)	1715, 2086
Eco81I	CCTNAGG	1 cut(s)	2079
Eco88I	CYCGRG	2 cut(s)	24, 2324
EcoO109I	RGGNCCY	1 cut(s)	1941
EcoRII	CCWGG	5 cut(s)	355, 1199, 1251, 1914, 2005
EcoT14I	CCWWGG	2 cut(s)	894, 929
EcoT22I	ATGCAT	1 cut(s)	2179
ErhI	CCWWGG	2 cut(s)	894, 929
Esp3I	CGTCTC	2 cut(s)	27, 274
FalI	AAGNNNNNCTT	6 cut(s)	1070, 1102, 1086, 1118, 2268, 2300
FaqI	GGGAC	4 cut(s)	81, 1301, 1805, 1856
FbaI	TGATCA	1 cut(s)	489
FblI	GTMKAC	2 cut(s)	1008, 1902
FokI	GGATG	8 cut(s)	809, 959, 1183, 1217, 1394, 1576, 1580, 1766
FspBI	CTAG	6 cut(s)	168, 404, 954, 1397, 1763, 2204
GlaI	GCGC	2 cut(s)	1558, 2280
GsuI	CTGGAG	1 cut(s)	1321
HaeII	RGCGCY	2 cut(s)	1560, 2282
HaeIII	GGCC	3 cut(s)	430, 900, 1919
HapII	CCGG	2 cut(s)	1448, 2118
HgaI	GACGC	3 cut(s)	1468, 1802, 2204
HhaI	GCGC	2 cut(s)	1559, 2281
Hin6I	GCGC	2 cut(s)	1557, 2279
HinP1I	GCGC	2 cut(s)	1557, 2279
HincII	GTYRAC	2 cut(s)	471, 662
HindII	GTYRAC	2 cut(s)	471, 662
HindIII	AAGCTT	3 cut(s)	1079, 1100, 1184
HpaI	GTTAAC	1 cut(s)	471
HpaII	CCGG	2 cut(s)	1448, 2118
HphI	GGTGA	3 cut(s)	488, 1676, 2313
Hpy166II	GTNNAC	4 cut(s)	471, 662, 1009, 1903
Hpy188I	TCNGA	8 cut(s)	274, 784, 1270, 1543, 1602, 1778, 2246, 2339
Hpy8I	GTNNAC	4 cut(s)	471, 662, 1009, 1903
Hpy99I	CGWCG	3 cut(s)	51, 269, 278
HpyAV	CCTTC	2 cut(s)	223, 2328
HpyCH4III	ACNGT	8 cut(s)	475, 989, 1013, 1156, 1274, 1705, 1815, 2144
HpyCH4IV	ACGT	6 cut(s)	20, 529, 546, 1351, 2001, 2031
HpyF10VI	GCNNNNNNNGC	9 cut(s)	248, 251, 427, 592, 614, 884, 1747, 2183, 2363
HpySE526I	ACGT	6 cut(s)	20, 529, 546, 1351, 2001, 2031
HspAI	GCGC	2 cut(s)	1557, 2279
Ksp22I	TGATCA	1 cut(s)	489
KspAI	GTTAAC	1 cut(s)	471
Kzo9I	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
LguI	GCTCTTC	2 cut(s)	1615, 1734
LmnI	GCTCC	4 cut(s)	400, 418, 955, 1450
LweI	GCATC	7 cut(s)	328, 388, 787, 937, 1195, 1205, 2164
MaeI	CTAG	6 cut(s)	168, 404, 954, 1397, 1763, 2204
MaeII	ACGT	6 cut(s)	20, 529, 546, 1351, 2001, 2031
MaeIII	GTNAC	4 cut(s)	1109, 1664, 2105, 2144
MalI	GATC	9 cut(s)	52, 172, 465, 491, 842, 935, 1524, 1980, 2037
MboI	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
MflI	RGATCY	1 cut(s)	170
MhlI	GDGCHC	2 cut(s)	370, 994
MlsI	TGGCCA	1 cut(s)	430
MluNI	TGGCCA	1 cut(s)	430
MlyI	GAGTC	6 cut(s)	7, 274, 1273, 1599, 2027, 2343
MmeI	TCCRAC	2 cut(s)	41, 297
Mox20I	TGGCCA	1 cut(s)	430
Mph1103I	ATGCAT	1 cut(s)	2179
MroXI	GAANNNNTTC	1 cut(s)	546
MscI	TGGCCA	1 cut(s)	430
MslI	CAYNNNNRTG	3 cut(s)	906, 1139, 1850
Msp20I	TGGCCA	1 cut(s)	430
MspA1I	CMGCKG	3 cut(s)	248, 887, 2132
MspCI	CTTAAG	2 cut(s)	500, 2285
MspI	CCGG	2 cut(s)	1448, 2118
MspR9I	CCNGG	6 cut(s)	357, 1201, 1253, 1916, 2007, 2119
MvaI	CCWGG	5 cut(s)	357, 1201, 1253, 1916, 2007
MvnI	CGCG	2 cut(s)	1479, 1796
MwoI	GCNNNNNNNGC	9 cut(s)	248, 251, 427, 592, 614, 884, 1747, 2183, 2363
NciI	CCSGG	1 cut(s)	2119
NcoI	CCATGG	1 cut(s)	929
NdeII	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
NlaIV	GGNNCC	4 cut(s)	420, 444, 1499, 1942
NmeAIII	GCCGAG	1 cut(s)	1613
NmuCI	GTSAC	2 cut(s)	1664, 2105
NsiI	ATGCAT	1 cut(s)	2179
NspI	RCATGY	3 cut(s)	980, 1727, 1741
PaeR7I	CTCGAG	1 cut(s)	24
PagI	TCATGA	1 cut(s)	1711
PciSI	GCTCTTC	2 cut(s)	1615, 1734
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PdmI	GAANNNNTTC	1 cut(s)	546
PfeI	GAWTC	9 cut(s)	260, 292, 329, 361, 626, 1265, 1319, 1487, 2241
PflMI	CCANNNNNTGG	2 cut(s)	1299, 1436
Ple19I	CGATCG	1 cut(s)	53
PleI	GAGTC	6 cut(s)	7, 274, 1273, 1598, 2026, 2343
PpsI	GAGTC	6 cut(s)	7, 274, 1273, 1598, 2026, 2343
Ppu21I	YACGTR	2 cut(s)	530, 2002
PpuMI	RGGWCCY	1 cut(s)	1941
PshAI	GACNNNNGTC	1 cut(s)	2017
PshBI	ATTAAT	1 cut(s)	1335
Psp5II	RGGWCCY	1 cut(s)	1941
Psp6I	CCWGG	5 cut(s)	355, 1199, 1251, 1914, 2005
PspGI	CCWGG	5 cut(s)	355, 1199, 1251, 1914, 2005
PspN4I	GGNNCC	4 cut(s)	420, 444, 1499, 1942
PspPI	GGNCC	4 cut(s)	898, 926, 1941, 2012
PspPPI	RGGWCCY	1 cut(s)	1941
PstI	CTGCAG	1 cut(s)	1264
PstNI	CAGNNNCTG	1 cut(s)	1268
PsuI	RGATCY	1 cut(s)	170
PvuI	CGATCG	1 cut(s)	53
PvuII	CAGCTG	2 cut(s)	887, 2132
RsaI	GTAC	1 cut(s)	2004
RsaNI	GTAC	1 cut(s)	2003
RseI	CAYNNNNRTG	3 cut(s)	906, 1139, 1850
SapI	GCTCTTC	2 cut(s)	1615, 1734
Sau3AI	GATC	9 cut(s)	50, 170, 463, 489, 840, 933, 1522, 1978, 2035
Sau96I	GGNCC	4 cut(s)	898, 926, 1941, 2012
SchI	GAGTC	6 cut(s)	7, 274, 1273, 1599, 2027, 2343
ScrFI	CCNGG	6 cut(s)	357, 1201, 1253, 1916, 2007, 2119
SduI	GDGCHC	2 cut(s)	370, 994
SfaNI	GCATC	7 cut(s)	328, 388, 787, 937, 1195, 1205, 2164
SfcI	CTRYAG	2 cut(s)	1260, 2095
Sfr274I	CTCGAG	1 cut(s)	24
SinI	GGWCC	3 cut(s)	926, 1941, 2012
SlaI	CTCGAG	1 cut(s)	24
SmiMI	CAYNNNNRTG	3 cut(s)	906, 1139, 1850
SmlI	CTYRAG	5 cut(s)	24, 500, 1232, 1982, 2285
SmoI	CTYRAG	5 cut(s)	24, 500, 1232, 1982, 2285
SnaBI	TACGTA	1 cut(s)	2002
SspI	AATATT	1 cut(s)	68
SspMI	CTAG	6 cut(s)	168, 404, 954, 1397, 1763, 2204
StyD4I	CCNGG	6 cut(s)	355, 1199, 1251, 1914, 2005, 2117
StyI	CCWWGG	2 cut(s)	894, 929
TaaI	ACNGT	8 cut(s)	475, 989, 1013, 1156, 1274, 1705, 1815, 2144
TaiI	ACGT	6 cut(s)	23, 532, 549, 1354, 2004, 2034
TaqI	TCGA	9 cut(s)	25, 49, 290, 312, 384, 540, 759, 843, 1785
TauI	GCSGC	5 cut(s)	245, 248, 254, 1211, 1922
TfiI	GAWTC	9 cut(s)	260, 292, 329, 361, 626, 1265, 1319, 1487, 2241
TscAI	CASTG	4 cut(s)	655, 948, 1371, 1978
TseFI	GTSAC	2 cut(s)	1664, 2105
Tsp45I	GTSAC	2 cut(s)	1664, 2105
TspDTI	ATGAA	5 cut(s)	735, 918, 1157, 1700, 1728
TspGWI	ACGGA	6 cut(s)	216, 253, 312, 322, 1679, 1922
TspRI	CASTG	4 cut(s)	655, 948, 1371, 1978
Van91I	CCANNNNNTGG	2 cut(s)	1299, 1436
Vha464I	CTTAAG	2 cut(s)	500, 2285
VpaK11BI	GGWCC	3 cut(s)	926, 1941, 2012
VspI	ATTAAT	1 cut(s)	1335
XapI	RAATTY	3 cut(s)	809, 1715, 1964
XceI	RCATGY	3 cut(s)	980, 1727, 1741
XhoI	CTCGAG	1 cut(s)	24
XmiI	GTMKAC	2 cut(s)	1008, 1902
XmnI	GAANNNNTTC	1 cut(s)	546
XspI	CTAG	6 cut(s)	168, 404, 954, 1397, 1763, 2204
Zsp2I	ATGCAT	1 cut(s)	2179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.