Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0004392

Physical Location & Seq

Genomic Coordinates Reverse (-)

20246303 .. 20253008

Gene Structure 6706 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G016780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGAAGCATGCTCCTCGCTTAGCCTTAGCCTTAGCCTTGGGCTTGCAAATTCCAAAGAGAGCCCTATTGGTCTCTCTCCTTATACTCAACATGCTCATGGCAACTCGCGTCATTCTCTCCCGCTCAGTCTCCCCACTCCTCCTCAAGCGAGCTCCTTATCCCCATTTTACCCCTCAACGCTTACCCAAATCCCATCCCTGTCCCCTCTGGTCTTCTTCCTTCTCCTTCTGCCTCACTCTCCTCCCTAAACCCGCCCCCACATCCCCTCCCTTCACCTCCCGACGACCTCGCTCTTCCCTCTCGATGGCCGCCTCTGCTGCCGTCGTCGACGAATCTCTCTCCTCCAATCCTCTCCTCCGGGACTTCGACTTCCCTCCCTTCGACGTCGTTGACGCCTCGCACGTGCGACCTGGAATTCGAGCCCTATTGAAGAAGCTCGAGTCTGATTTGGAGGAGTTGGAGCGCACGGTGGAGCCTAACTGGCCGAAGCTGGTGGTGCCGTTGGAGAAGATCGTTGACCGGTTGACTGTGGTTTGGGGAGTAGTCAACCACCTCAAAGCTGTTAAGGACTCGTCGGAGCTTCGTGCTGCTATTGAAGAAGTGCAACCGGAGAAAGTGAAGTTTTATCTTAGGTTGGGACAAAGCAAGCCGATTTACAATGCGTTTAAAGCAATTCAAGACTCTCCTGATTGGAAGACATTGAGTGATGCTCAGAAACGTATAGTTGAAAACCAAATAAAGGAGGCAGTTCTTAGTGGAGTTGCCCTTGAGGATGATAAAAGAGAGGCTTTTAACAAAATTGAACAGGAACTGGAAAGACTGTCTCAAAAATTTGGGGAGAATGTTCTGGATGCCACAAAGAAATTTGAAAAGTTAATCACCGATAAGAAAGAAATTGATGGATTGCCAGCTACTGCTCTAGGATTGGCTGCACAAACAGCAGTGTCAAAGGGGCATGAAAATGCGACTGCTGAGAATGGGCCTTGGGTATTTACATTAGATGGTCCAAGTTTTCTCTCTGTTATGCAACATGCTCGAAACCGGTCTCTGCGCGAGGAAATATACCGTGCTTATGTAACTCGTGCATCTAGTGGAGAGCTGGATAATACTGCAATTATTGACTCAATCTTGAAGCTTAGGTTGGAAAAGGCTAAGCTTCTGAATTACAACAACTATGCTGAGGTAAGCATGGCAACCAAAATGGCTACTGTTGAAAAAGCAGAAGAGCTTCTAGAGAAACTTCGAAGTGCTTCTTGGAAGGCTGGAGTTCAAGATATGGAAGACCTTAAGAATTTCTGCAAAAGCCAAGATGCTCCAGAAGCTGATGATTTGAACCACTGGGACATTAACTTTTGGAGTGAGAGGCTTCGGGAGTCCAAATTTGATATCAACGAGGAAGAATTGCGCCCCTATTTCTCCTTGCCAAAGGTTATGGATGGCCTGTTTAGCCTAGCGAACAAGATTTTTGGAATTGACATTGAACCAGCTGATGGTTTAGCTCCGGTTTGGAACAATGATGTTAGATTCTATCGTGTCAAAGATTCTTCTGGGGATCCTATTGCATATTTTTACTTCGATCCCTATACTCGTCCATCTGAGAAAAGGGGAGGTGCGTGGATGGATGAAGTACTTGCTAGGAGCCGTGTATTGTCACGTGATGGTACCTCTCCAAGGTTGCCTGTGGCACACATGGTATGCAATCAATCACCACCAGTGGGAGACAAACCCAGCTTGATGACCTTCCGCGAGGTTGAGACCGTCTTCCATGAATTTGGTCATGCACTTCAGCATATGCTGACTAAGCAAGATGAGGGTCTTGTTGCTGGTATCCGAGGGGTAGAGTGGGATGCTGTGAATTGCCCTCACAGTTCATGGAAAATTGGTGTTACCACAGGTAATTTAGACACTTTAATGAGCATTGCTAAGCATTATGAAACTGCGGAAACCCTTCCAGAAGAAGTATTCCTGAAGCTTCTTGCAGCTAGGACTTTTCGTGCCGGCTCCCTAAGCCTTCGACAGATAAGATTTGCAATTGTAGATCTGAAGCTGCACACGGATTACATACCTGGTGGGTCAGAATCCATTTATGATGTTGATCAGAGGGTTAGTGAAAAAACACAAGTGATCCCCCCACTTCCAGAAGATAGGTTCCTTTGTGGTTTCAGCCATATCTTTGCAGGTGGATATGCAGCTGGGTACTATAGTTACAAGTGGGCTGAGGTCTTGTCTGCTGATGCTTTCTCTGCATTTGAGGATGCTGGATTGGATAATAGCAAGGTAAGCTGTAAAGAAACCGGAAACAAGTTCCGAGAAACGATCCTTGCTCTTGGAGGCGGGAAAGCACCACTGGACGTTTTTGTGGAATTCCGAGGACGTGAGCCTTCACCGGAGCCACTGCTCAGGCACAATGGCCTATTACAAGTTGCTACTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031974 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M3

Protein Analysis

811

Amino Acids

90.81

Weight (kDa)

6.25

Isoelectric Point (pI)

47.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP_N	PF19310	139 - 261	2.7e-57	Neurolysin/Thimet oligopeptidase, N-terminal domain
Peptidase_M3	PF01432	341 - 804	3e-123	Peptidase family M3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10540 AT5G65620 AT5G65620
fragaria_vesca	FvH4_1g19050 FvH4_1g19050 FvH4_1g19050
malus_domestica	MD01G1044200.v1.1
prunus_persica	Prupe.6G167100_v2.0.a1 Prupe.6G167100_v2.0.a1 Prupe.6G209200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0110061 RchiOBHm_Chr7g0233391
rosa_laevigata	RLG00000001272 RLG00000017783
rosa_multiflora	Rmu_co8163522.1_g000001 Rmu_co8337137.1_g000001 Rmu_sc0008806.1_g000009 Rmu_sc0009935.1_g000003 Rmu_sc0013750.1_g000001 Rmu_sc0024311.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133750
rosa_rugosa	Rorug02G0165400 Rorug02G0165400
rosa_samantha	Rh2AG217200 Rh2BG227200 Rh2CG219400 Rh2DG222900 Rh7CG455700
rosa_wichuraiana	Rw0G010890 Rw2G016780 Rw7G035960 Rw7G035980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2168
AatII	GACGTC	1 cut(s)	387
Acc36I	ACCTGC	1 cut(s)	2168
Acc65I	GGTACC	1 cut(s)	1661
AccB1I	GGYRCC	2 cut(s)	496, 1661
AccB7I	CCANNNNNTGG	1 cut(s)	1490
AccBSI	CCGCTC	1 cut(s)	123
AccI	GTMKAC	1 cut(s)	327
AccII	CGCG	3 cut(s)	108, 1053, 1746
AciI	CCGC	6 cut(s)	121, 252, 309, 1744, 1940, 2334
AclWI	GGATC	5 cut(s)	1547, 1560, 1571, 2119, 2311
AcoI	YGGCCR	2 cut(s)	306, 482
AcsI	RAATTY	8 cut(s)	48, 414, 830, 863, 1291, 1379, 1769, 2363
AcuI	CTGAAG	3 cut(s)	1769, 1988, 2063
AcvI	CACGTG	2 cut(s)	403, 1655
AcyI	GRCGYC	2 cut(s)	384, 393
AfaI	GTAC	3 cut(s)	1629, 1663, 2198
AfiI	CCNNNNNNNGG	5 cut(s)	304, 739, 1490, 1671, 1715
AflII	CTTAAG	1 cut(s)	1286
AgeI	ACCGGT	2 cut(s)	519, 1041
AjiI	CACGTC	1 cut(s)	2375
AjnI	CCWGG	2 cut(s)	409, 2065
AjuI	GAANNNNNNNTTGG	4 cut(s)	1124, 1156, 1237, 1269
Alw21I	GWGCWC	1 cut(s)	154
Alw26I	GTCTC	6 cut(s)	76, 133, 828, 1050, 1713, 1748
AlwI	GGATC	5 cut(s)	1547, 1560, 1571, 2119, 2311
AlwNI	CAGNNNCTG	3 cut(s)	811, 914, 1322
Ama87I	CYCGRG	1 cut(s)	437
AoxI	GGCC	5 cut(s)	306, 482, 980, 1438, 2410
ApeKI	GCWGC	6 cut(s)	317, 587, 929, 1979, 2047, 2189
ApoI	RAATTY	8 cut(s)	48, 414, 830, 863, 1291, 1379, 1769, 2363
ArsI	GACNNNNNNTTYG	2 cut(s)	399, 431
AsiGI	ACCGGT	2 cut(s)	519, 1041
Asp700I	GAANNNNTTC	3 cut(s)	1227, 1249, 1947
Asp718I	GGTACC	1 cut(s)	1661
AspLEI	GCGC	3 cut(s)	465, 1053, 1407
AspS9I	GGNCC	2 cut(s)	980, 1004
AsuC2I	CCSGG	1 cut(s)	359
AsuHPI	GGTGA	4 cut(s)	265, 871, 1698, 2376
AsuII	TTCGAA	1 cut(s)	1243
AvaI	CYCGRG	1 cut(s)	437
AvaII	GGWCC	1 cut(s)	1004
BaeI	ACNNNNGTAYC	2 cut(s)	2188, 2221
BamHI	GGATCC	1 cut(s)	1552
BanI	GGYRCC	2 cut(s)	496, 1661
BanII	GRGCYC	3 cut(s)	64, 154, 424
BauI	CACGAG	1 cut(s)	1080
BbrPI	CACGTG	2 cut(s)	403, 1655
BbsI	GAAGAC	4 cut(s)	204, 701, 1287, 1753
Bbv12I	GWGCWC	1 cut(s)	154
BbvCI	CCTCAGC	2 cut(s)	1179, 2217
BbvI	GCAGC	6 cut(s)	304, 574, 916, 1991, 2034, 2201
BccI	CCATC	9 cut(s)	201, 298, 893, 995, 1430, 1484, 1600, 1612, 1652
BceAI	ACGGC	3 cut(s)	305, 484, 1626
BcgI	CGANNNNNNTGC	3 cut(s)	30, 1016, 1050
BciT130I	CCWGG	2 cut(s)	411, 2067
BciVI	GTATCC	1 cut(s)	1838
BclI	TGATCA	1 cut(s)	2095
BcnI	CCSGG	1 cut(s)	359
BcoDI	GTCTC	6 cut(s)	76, 133, 828, 1050, 1713, 1748
BfaI	CTAG	6 cut(s)	920, 1089, 1232, 1451, 1635, 1983
BfmI	CTRYAG	1 cut(s)	2200
BfrI	CTTAAG	1 cut(s)	1286
BfuAI	ACCTGC	1 cut(s)	2168
BfuI	GTATCC	1 cut(s)	1838
BglI	GCCNNNNNGGC	1 cut(s)	481
BglII	AGATCT	1 cut(s)	2038
BisI	GCNGC	7 cut(s)	309, 318, 588, 930, 1980, 2048, 2190
BlpI	GCTNAGC	3 cut(s)	19, 1152, 1923
BlsI	GCNGC	7 cut(s)	310, 319, 589, 931, 1981, 2049, 2191
BmcAI	AGTACT	1 cut(s)	1629
Bme1390I	CCNGG	3 cut(s)	359, 411, 2067
Bme18I	GGWCC	1 cut(s)	1004
BmeT110I	CYCGRG	1 cut(s)	437
BmgBI	CACGTC	1 cut(s)	2375
BmgT120I	GGNCC	2 cut(s)	980, 1004
BmiI	GGNNCC	8 cut(s)	474, 498, 1554, 1640, 1663, 2002, 2150, 2391
BmrFI	CCNGG	3 cut(s)	359, 411, 2067
BmrI	ACTGGG	1 cut(s)	1348
BmsI	GCATC	7 cut(s)	697, 841, 1094, 1300, 1837, 2224, 2245
BmuI	ACTGGG	1 cut(s)	1348
BpiI	GAAGAC	4 cut(s)	204, 701, 1287, 1753
BplI	GAGNNNNNCTC	2 cut(s)	694, 726
BpmI	CTGGAG	2 cut(s)	1284, 1299
Bpu10I	CCTNAGC	7 cut(s)	25, 31, 1136, 1179, 2006, 2217, 2399
Bpu1102I	GCTNAGC	3 cut(s)	19, 1152, 1923
Bpu14I	TTCGAA	1 cut(s)	1243
BpuEI	CTTGAG	2 cut(s)	128, 788
BpuMI	CCSGG	1 cut(s)	359
BsaAI	YACGTR	2 cut(s)	403, 1655
BsaBI	GATNNNNATC	1 cut(s)	2094
BsaHI	GRCGYC	2 cut(s)	384, 393
BsaI	GGTCTC	3 cut(s)	76, 1050, 1748
BsaJI	CCNNGG	5 cut(s)	36, 983, 1670, 1831, 2368
BsaWI	WCCGGW	6 cut(s)	519, 607, 1041, 1501, 2294, 2386
BsaXI	ACNNNNNCTCC	2 cut(s)	250, 280
Bsc4I	CCNNNNNNNGG	5 cut(s)	304, 739, 1490, 1671, 1715
Bse118I	RCCGGY	3 cut(s)	519, 1041, 1997
Bse1I	ACTGG	5 cut(s)	485, 816, 1343, 1712, 2352
Bse3DI	GCAATG	1 cut(s)	1917
Bse8I	GATNNNNATC	1 cut(s)	2094
BseBI	CCWGG	2 cut(s)	411, 2067
BseDI	CCNNGG	5 cut(s)	36, 983, 1670, 1831, 2368
BseGI	GGATG	9 cut(s)	193, 260, 778, 856, 1441, 1623, 1627, 1852, 2260
BseJI	GATNNNNATC	1 cut(s)	2094
BseLI	CCNNNNNNNGG	5 cut(s)	304, 739, 1490, 1671, 1715
BseMI	GCAATG	1 cut(s)	1917
BseMII	CTCAG	7 cut(s)	138, 725, 963, 1170, 1587, 2208, 2413
BseNI	ACTGG	5 cut(s)	485, 816, 1343, 1712, 2352
BseRI	GAGGAG	6 cut(s)	128, 131, 230, 331, 344, 467
BseXI	GCAGC	6 cut(s)	304, 574, 916, 1991, 2034, 2201
BseYI	CCCAGC	2 cut(s)	1727, 2192
BsgI	GTGCAG	2 cut(s)	915, 2033
Bsh1236I	CGCG	3 cut(s)	108, 1053, 1746
BshFI	GGCC	5 cut(s)	308, 484, 982, 1440, 2412
BshNI	GGYRCC	2 cut(s)	496, 1661
BshTI	ACCGGT	2 cut(s)	519, 1041
BsiHKAI	GWGCWC	1 cut(s)	154
BsiHKCI	CYCGRG	1 cut(s)	437
BsiSI	CCGG	8 cut(s)	358, 520, 608, 1042, 1502, 1998, 2295, 2387
BslFI	GGGAC	4 cut(s)	186, 374, 651, 1355
BslI	CCNNNNNNNGG	5 cut(s)	304, 739, 1490, 1671, 1715
BsmAI	GTCTC	6 cut(s)	76, 133, 828, 1050, 1713, 1748
BsmFI	GGGAC	4 cut(s)	186, 374, 651, 1355
BsnI	GGCC	5 cut(s)	308, 484, 982, 1440, 2412
Bso31I	GGTCTC	3 cut(s)	76, 1050, 1748
BsoBI	CYCGRG	1 cut(s)	437
Bsp119I	TTCGAA	1 cut(s)	1243
Bsp1286I	GDGCHC	3 cut(s)	64, 154, 424
Bsp143I	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
Bsp1720I	GCTNAGC	3 cut(s)	19, 1152, 1923
BspACI	CCGC	6 cut(s)	121, 252, 309, 1744, 1940, 2334
BspANI	GGCC	5 cut(s)	308, 484, 982, 1440, 2412
BspCNI	CTCAG	7 cut(s)	137, 724, 964, 1171, 1588, 2209, 2412
BspFNI	CGCG	3 cut(s)	108, 1053, 1746
BspLI	GGNNCC	8 cut(s)	474, 498, 1554, 1640, 1663, 2002, 2150, 2391
BspMI	ACCTGC	1 cut(s)	2168
BspPI	GGATC	5 cut(s)	1547, 1560, 1571, 2119, 2311
BspQI	GCTCTTC	2 cut(s)	298, 1218
BspT104I	TTCGAA	1 cut(s)	1243
BspT107I	GGYRCC	2 cut(s)	496, 1661
BspTI	CTTAAG	1 cut(s)	1286
BspTNI	GGTCTC	3 cut(s)	76, 1050, 1748
BsrBI	CCGCTC	1 cut(s)	123
BsrDI	GCAATG	1 cut(s)	1917
BsrFI	RCCGGY	3 cut(s)	519, 1041, 1997
BsrI	ACTGG	5 cut(s)	485, 816, 1343, 1712, 2352
BssAI	RCCGGY	3 cut(s)	519, 1041, 1997
BssECI	CCNNGG	5 cut(s)	36, 983, 1670, 1831, 2368
BssMI	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
BssNI	GRCGYC	2 cut(s)	384, 393
BssSI	CACGAG	1 cut(s)	1080
BssT1I	CCWWGG	3 cut(s)	36, 983, 1670
Bst2BI	CACGAG	1 cut(s)	1080
Bst2UI	CCWGG	2 cut(s)	411, 2067
Bst4CI	ACNGT	7 cut(s)	469, 529, 822, 1067, 1210, 1759, 1868
Bst6I	CTCTTC	2 cut(s)	298, 1218
BstACI	GRCGYC	2 cut(s)	384, 393
BstAFI	CTTAAG	1 cut(s)	1286
BstBAI	YACGTR	2 cut(s)	403, 1655
BstBI	TTCGAA	1 cut(s)	1243
BstC8I	GCNNGC	6 cut(s)	9, 44, 150, 647, 909, 1999
BstENI	CCTNNNNNAGG	1 cut(s)	1669
BstF5I	GGATG	9 cut(s)	193, 260, 778, 856, 1441, 1623, 1627, 1852, 2260
BstFNI	CGCG	3 cut(s)	108, 1053, 1746
BstHHI	GCGC	3 cut(s)	465, 1053, 1407
BstKTI	GATC	7 cut(s)	513, 1555, 1579, 2041, 2098, 2127, 2319
BstMAI	GTCTC	6 cut(s)	76, 133, 828, 1050, 1713, 1748
BstMBI	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
BstNI	CCWGG	2 cut(s)	411, 2067
BstNSI	RCATGY	3 cut(s)	11, 94, 1034
BstSCI	CCNGG	3 cut(s)	357, 409, 2065
BstSFI	CTRYAG	1 cut(s)	2200
BstUI	CGCG	3 cut(s)	108, 1053, 1746
BstV1I	GCAGC	6 cut(s)	304, 574, 916, 1991, 2034, 2201
BstV2I	GAAGAC	4 cut(s)	204, 701, 1287, 1753
BstX2I	RGATCY	2 cut(s)	1552, 2038
BstXI	CCANNNNNNTGG	1 cut(s)	1772
BstYI	RGATCY	2 cut(s)	1552, 2038
BsuI	GTATCC	1 cut(s)	1838
BsuRI	GGCC	5 cut(s)	308, 484, 982, 1440, 2412
BtrI	CACGTC	1 cut(s)	2375
BtsCI	GGATG	9 cut(s)	193, 260, 778, 856, 1441, 1623, 1627, 1852, 2260
BtsI	GCAGTG	2 cut(s)	948, 2393
BtsIMutI	CAGTG	5 cut(s)	948, 1336, 1719, 2345, 2393
BveI	ACCTGC	1 cut(s)	2168
Cac8I	GCNNGC	6 cut(s)	9, 44, 150, 647, 909, 1999
CaiI	CAGNNNCTG	3 cut(s)	811, 914, 1322
CfoI	GCGC	3 cut(s)	465, 1053, 1407
Cfr10I	RCCGGY	3 cut(s)	519, 1041, 1997
Cfr13I	GGNCC	2 cut(s)	980, 1004
CseI	GACGC	2 cut(s)	97, 401
CsiI	ACCWGGT	1 cut(s)	2065
Csp6I	GTAC	3 cut(s)	1628, 1662, 2197
CspAI	ACCGGT	2 cut(s)	519, 1041
CviQI	GTAC	3 cut(s)	1628, 1662, 2197
DpnI	GATC	7 cut(s)	512, 1554, 1578, 2040, 2097, 2126, 2318
DpnII	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
DraI	TTTAAA	1 cut(s)	667
EaeI	YGGCCR	2 cut(s)	306, 482
Eam1104I	CTCTTC	2 cut(s)	298, 1218
EarI	CTCTTC	2 cut(s)	298, 1218
Ecl136II	GAGCTC	1 cut(s)	152
Eco130I	CCWWGG	3 cut(s)	36, 983, 1670
Eco24I	GRGCYC	3 cut(s)	64, 154, 424
Eco31I	GGTCTC	3 cut(s)	76, 1050, 1748
Eco32I	GATATC	1 cut(s)	1387
Eco47I	GGWCC	1 cut(s)	1004
Eco53kI	GAGCTC	1 cut(s)	152
Eco57I	CTGAAG	3 cut(s)	1769, 1988, 2063
Eco72I	CACGTG	2 cut(s)	403, 1655
Eco88I	CYCGRG	1 cut(s)	437
EcoICRI	GAGCTC	1 cut(s)	152
EcoNI	CCTNNNNNAGG	1 cut(s)	1669
EcoRI	GAATTC	2 cut(s)	414, 2363
EcoRII	CCWGG	2 cut(s)	409, 2065
EcoRV	GATATC	1 cut(s)	1387
EcoT14I	CCWWGG	3 cut(s)	36, 983, 1670
EcoT38I	GRGCYC	3 cut(s)	64, 154, 424
ErhI	CCWWGG	3 cut(s)	36, 983, 1670
FalI	AAGNNNNNCTT	4 cut(s)	772, 804, 1237, 1269
FaqI	GGGAC	4 cut(s)	186, 374, 651, 1355
FauI	CCCGC	3 cut(s)	128, 259, 2327
FauNDI	CATATG	1 cut(s)	1791
FbaI	TGATCA	1 cut(s)	2095
FblI	GTMKAC	1 cut(s)	327
Fnu4HI	GCNGC	7 cut(s)	309, 318, 588, 930, 1980, 2048, 2190
FokI	GGATG	9 cut(s)	180, 247, 785, 863, 1448, 1630, 1634, 1859, 2267
FriOI	GRGCYC	3 cut(s)	64, 154, 424
Fsp4HI	GCNGC	7 cut(s)	309, 318, 588, 930, 1980, 2048, 2190
FspBI	CTAG	6 cut(s)	920, 1089, 1232, 1451, 1635, 1983
GlaI	GCGC	3 cut(s)	464, 1052, 1406
GluI	GCNGC	7 cut(s)	309, 318, 588, 930, 1980, 2048, 2190
GsaI	CCCAGC	2 cut(s)	1731, 2196
GsuI	CTGGAG	2 cut(s)	1284, 1299
HaeIII	GGCC	5 cut(s)	308, 484, 982, 1440, 2412
HapII	CCGG	8 cut(s)	358, 520, 608, 1042, 1502, 1998, 2295, 2387
HgaI	GACGC	2 cut(s)	97, 401
HhaI	GCGC	3 cut(s)	465, 1053, 1407
Hin1I	GRCGYC	2 cut(s)	384, 393
Hin6I	GCGC	3 cut(s)	463, 1051, 1405
HinP1I	GCGC	3 cut(s)	463, 1051, 1405
HincII	GTYRAC	5 cut(s)	328, 391, 517, 525, 547
HindII	GTYRAC	5 cut(s)	328, 391, 517, 525, 547
HindIII	AAGCTT	3 cut(s)	1133, 1154, 1970
HinfI	GANTC	9 cut(s)	332, 440, 569, 680, 1121, 1373, 1524, 1541, 2078
HpaII	CCGG	8 cut(s)	358, 520, 608, 1042, 1502, 1998, 2295, 2387
HphI	GGTGA	4 cut(s)	265, 871, 1698, 2376
Hpy166II	GTNNAC	5 cut(s)	328, 391, 517, 525, 547
Hpy8I	GTNNAC	5 cut(s)	328, 391, 517, 525, 547
Hpy99I	CGWCG	7 cut(s)	285, 326, 329, 332, 386, 389, 577
HpyAV	CCTTC	9 cut(s)	229, 235, 280, 388, 1252, 1750, 1958, 2021, 2391
HpyCH4III	ACNGT	7 cut(s)	469, 529, 822, 1067, 1210, 1759, 1868
HpyCH4IV	ACGT	6 cut(s)	384, 402, 718, 1654, 2352, 2374
HpySE526I	ACGT	6 cut(s)	384, 402, 718, 1654, 2352, 2374
Hsp92I	GRCGYC	2 cut(s)	384, 393
HspAI	GCGC	3 cut(s)	463, 1051, 1405
KpnI	GGTACC	1 cut(s)	1665
KroI	GCCGGC	1 cut(s)	1997
KroNI	GCCGGC	1 cut(s)	1999
Ksp22I	TGATCA	1 cut(s)	2095
Kzo9I	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
LguI	GCTCTTC	2 cut(s)	298, 1218
Lsp1109I	GCAGC	6 cut(s)	304, 574, 916, 1991, 2034, 2201
LweI	GCATC	7 cut(s)	697, 841, 1094, 1300, 1837, 2224, 2245
MabI	ACCWGGT	1 cut(s)	2065
MaeI	CTAG	6 cut(s)	920, 1089, 1232, 1451, 1635, 1983
MaeII	ACGT	6 cut(s)	384, 402, 718, 1654, 2352, 2374
MaeIII	GTNAC	4 cut(s)	1075, 1650, 1885, 2204
MalI	GATC	7 cut(s)	512, 1554, 1578, 2040, 2097, 2126, 2318
MbiI	CCGCTC	1 cut(s)	123
MboI	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
MfeI	CAATTG	1 cut(s)	2031
MflI	RGATCY	2 cut(s)	1552, 2038
MhlI	GDGCHC	3 cut(s)	64, 154, 424
MlyI	GAGTC	5 cut(s)	449, 563, 674, 1115, 1382
MmeI	TCCRAC	4 cut(s)	438, 483, 555, 1122
MroNI	GCCGGC	1 cut(s)	1997
MroXI	GAANNNNTTC	3 cut(s)	1227, 1249, 1947
MseI	TTAA	7 cut(s)	564, 666, 792, 875, 1287, 1347, 1910
MslI	CAYNNNNRTG	3 cut(s)	95, 960, 1910
MspA1I	CMGCKG	2 cut(s)	1487, 2192
MspCI	CTTAAG	1 cut(s)	1286
MspI	CCGG	8 cut(s)	358, 520, 608, 1042, 1502, 1998, 2295, 2387
MspR9I	CCNGG	3 cut(s)	359, 411, 2067
MunI	CAATTG	1 cut(s)	2031
MvaI	CCWGG	2 cut(s)	411, 2067
MvnI	CGCG	3 cut(s)	108, 1053, 1746
NaeI	GCCGGC	1 cut(s)	1999
NciI	CCSGG	1 cut(s)	359
NdeI	CATATG	1 cut(s)	1791
NdeII	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
NgoMIV	GCCGGC	1 cut(s)	1997
NlaIV	GGNNCC	8 cut(s)	474, 498, 1554, 1640, 1663, 2002, 2150, 2391
NmuCI	GTSAC	1 cut(s)	1650
NspI	RCATGY	3 cut(s)	11, 94, 1034
NspV	TTCGAA	1 cut(s)	1243
PaeI	GCATGC	1 cut(s)	11
PaeR7I	CTCGAG	1 cut(s)	437
PaqCI	CACCTGC	1 cut(s)	2168
PciSI	GCTCTTC	2 cut(s)	298, 1218
PcsI	WCGNNNNNNNCGW	1 cut(s)	399
PdiI	GCCGGC	1 cut(s)	1999
PdmI	GAANNNNTTC	3 cut(s)	1227, 1249, 1947
PfeI	GAWTC	4 cut(s)	332, 1524, 1541, 2078
PflMI	CCANNNNNTGG	1 cut(s)	1490
PfoI	TCCNGGA	1 cut(s)	357
PinAI	ACCGGT	2 cut(s)	519, 1041
PkrI	GCNGC	7 cut(s)	310, 319, 589, 931, 1981, 2049, 2191
PleI	GAGTC	5 cut(s)	448, 563, 674, 1115, 1381
PmaCI	CACGTG	2 cut(s)	403, 1655
PmlI	CACGTG	2 cut(s)	403, 1655
PpsI	GAGTC	5 cut(s)	448, 563, 674, 1115, 1381
Ppu21I	YACGTR	2 cut(s)	403, 1655
Psp124BI	GAGCTC	1 cut(s)	154
Psp6I	CCWGG	2 cut(s)	409, 2065
PspCI	CACGTG	2 cut(s)	403, 1655
PspFI	CCCAGC	2 cut(s)	1727, 2192
PspGI	CCWGG	2 cut(s)	409, 2065
PspN4I	GGNNCC	8 cut(s)	474, 498, 1554, 1640, 1663, 2002, 2150, 2391
PspPI	GGNCC	2 cut(s)	980, 1004
PspXI	VCTCGAGB	1 cut(s)	437
PstNI	CAGNNNCTG	3 cut(s)	811, 914, 1322
PsuI	RGATCY	2 cut(s)	1552, 2038
PvuII	CAGCTG	2 cut(s)	1487, 2192
RsaI	GTAC	3 cut(s)	1629, 1663, 2198
RsaNI	GTAC	3 cut(s)	1628, 1662, 2197
RseI	CAYNNNNRTG	3 cut(s)	95, 960, 1910
SacI	GAGCTC	1 cut(s)	154
SalI	GTCGAC	1 cut(s)	326
SapI	GCTCTTC	2 cut(s)	298, 1218
SaqAI	TTAA	7 cut(s)	564, 666, 792, 875, 1287, 1347, 1910
SatI	GCNGC	7 cut(s)	309, 318, 588, 930, 1980, 2048, 2190
Sau3AI	GATC	7 cut(s)	510, 1552, 1576, 2038, 2095, 2124, 2316
Sau96I	GGNCC	2 cut(s)	980, 1004
ScaI	AGTACT	1 cut(s)	1629
SchI	GAGTC	5 cut(s)	449, 563, 674, 1115, 1382
ScrFI	CCNGG	3 cut(s)	359, 411, 2067
SduI	GDGCHC	3 cut(s)	64, 154, 424
SexAI	ACCWGGT	1 cut(s)	2065
SfaNI	GCATC	7 cut(s)	697, 841, 1094, 1300, 1837, 2224, 2245
SfcI	CTRYAG	1 cut(s)	2200
Sfr274I	CTCGAG	1 cut(s)	437
SfuI	TTCGAA	1 cut(s)	1243
SgrDI	CGTCGACG	1 cut(s)	326
SinI	GGWCC	1 cut(s)	1004
SlaI	CTCGAG	1 cut(s)	437
SmiMI	CAYNNNNRTG	3 cut(s)	95, 960, 1910
SmlI	CTYRAG	4 cut(s)	143, 437, 767, 1286
SmoI	CTYRAG	4 cut(s)	143, 437, 767, 1286
SphI	GCATGC	1 cut(s)	11
SsiI	CCGC	6 cut(s)	121, 252, 309, 1744, 1940, 2334
SspMI	CTAG	6 cut(s)	920, 1089, 1232, 1451, 1635, 1983
SstI	GAGCTC	1 cut(s)	154
StyD4I	CCNGG	3 cut(s)	357, 409, 2065
StyI	CCWWGG	3 cut(s)	36, 983, 1670
TaaI	ACNGT	7 cut(s)	469, 529, 822, 1067, 1210, 1759, 1868
TaiI	ACGT	6 cut(s)	387, 405, 721, 1657, 2355, 2377
TatI	WGTACW	1 cut(s)	1627
TauI	GCSGC	1 cut(s)	311
TfiI	GAWTC	4 cut(s)	332, 1524, 1541, 2078
Tru1I	TTAA	7 cut(s)	564, 666, 792, 875, 1287, 1347, 1910
Tru9I	TTAA	7 cut(s)	564, 666, 792, 875, 1287, 1347, 1910
TscAI	CASTG	5 cut(s)	948, 1343, 1719, 2352, 2400
TseFI	GTSAC	1 cut(s)	1650
TseI	GCWGC	6 cut(s)	317, 587, 929, 1979, 2047, 2189
Tsp45I	GTSAC	1 cut(s)	1650
TspDTI	ATGAA	6 cut(s)	17, 972, 1638, 1782, 1860, 1947
TspGWI	ACGGA	1 cut(s)	2069
TspRI	CASTG	5 cut(s)	948, 1343, 1719, 2352, 2400
Van91I	CCANNNNNTGG	1 cut(s)	1490
Vha464I	CTTAAG	1 cut(s)	1286
VpaK11BI	GGWCC	1 cut(s)	1004
XagI	CCTNNNNNAGG	1 cut(s)	1669
XapI	RAATTY	8 cut(s)	48, 414, 830, 863, 1291, 1379, 1769, 2363
XbaI	TCTAGA	1 cut(s)	1231
XceI	RCATGY	3 cut(s)	11, 94, 1034
XhoI	CTCGAG	1 cut(s)	437
XmiI	GTMKAC	1 cut(s)	327
XmnI	GAANNNNTTC	3 cut(s)	1227, 1249, 1947
XspI	CTAG	6 cut(s)	920, 1089, 1232, 1451, 1635, 1983
ZraI	GACGTC	1 cut(s)	385
ZrmI	AGTACT	1 cut(s)	1629

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.