Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004392

Physical Location & Seq

Genomic Coordinates Forward (+)

14355582 .. 14363310

Gene Structure 7729 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1044200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGAAGCATATTATCTTACGCAACAGAGTCTTATTGGCTTCTCTTCTTGTACTCAACGTGCTCATGGCAACTCGCGTCATCCTCTCTCGCTCAGTCCACCCTATCCTTAAACGCCCTCCTCCTTTTCCCCACTTTGCCCCCAAACGTTTCCCCAAATCTCATCCTTGCCCCCTCTGGTCTCCCTCATTCTCCTTCTGCCTCGCTACTCCCCACAAACCCACCACTCCCACCATTCCATCGTCACTCTCCACTCGCTCTTGCTCCTCCCTCTCTGCCACAGCCATGGCCGCCTCCGCCGCCGCAGACGAAGCTCTCGCAGCCAACCCCCTCCTCCAAGACTTTGATTTCCCTCCCTTCGACGTCATCGACGCCCAGCACGTCCGCCCTGGGATTCGCGCCCTCTTGAAGAAGCTCGAGGGTGATTTGGAGGAATTAGAGCGCACGGTGGAGCCCACGTGGACGAAGCTGGTCGTGCCGCTAGAGAAGATCTTTGACCGATTATCTGTGGTTTGGGGATTAGTCAACCACCTCAAGGCTGTTAAGGACTCCTCCGAGCTACGTTCTGCTATTGAAGAAGTCCAGCCGGAGAAAGTTAAGTTTGAGCTTAGGTTGAGGCAGAGTAAACCAATTTACAATGCATTTAAAGCTATTCGAGAATCTCCTGATTGGCAGACATTGAGTGATGCTCGCAAACGTATAGTTGAAGCCCAAATAAAGGAGGCAGTTTTGAGTGGTGTTTCTCTTGAGGATGACAAAAGAGATGAATTCAACAAAATTGAACAGGAACTAGAAAGACTGTCTCAAAAATTTTCAGACAATGTTTTGGATGCAACAAAGAAGTTTGAAAAGTTAATTACTGATAAGAATGAAATTGAAGGATTGCCAGCCACTGCTCTGGGAATGGCTGCACAAACAGCGGTGTCAAAGGGGCATGAAAATGCAAATGCTGAGAATGGGCCTTGGATTATTACATTGGACGGCCCAAGTTTCCTCTCTGTCATGCAACATGCTCGAAACCGGGCTTTGCGTGAGGAAATATACCGTGCTTATGTAGCTCGTGCATCCAGTGGAGATTTGGATAATACTGCTATCATTGACTCGATTTTGAAACTTAGGTTGGAAAAAGCGAAACTTCTGAATTACAATAACTATGCTGAGGTAAGCATGGCAACCAAAATGGCTACTGTTGAAAAAGCAGAGGAGCTCCTAGAAAAGCTTCGAAGTGCTTCATGGGATGCTGCAGTTCAAGATATGGAAGATCTTAAGAATTTTTCCAAGAGTCAAGATGCTCCTGAAGCTGATGATTTGAATCACTGGGACACCAGCTTTTGGAGTGAGAGGCTTCGTGAGTCAAAATTTGACATCAATGAGGAAGAATTGCGTCCATATTTCTCCTTGCCCAAGGTCATGGATGGCCTATTTAGACTAGCAAACATGCTTTTTGGAATTGATATTGAGCCGGCTGATGGTCTAGCGCCGGTTTGGAATAATGATGTTACATTCTATCGCATCAAAGATTCTTCTGGGAGTCCCATTGCATACTTCTATTTTGATCCATACACTCGTCCATCTGAGAAACGGGGAGGTGCATGGATGGATGAAGTTCTTGCTAGGAGTCATGTCTTGTCACGTGATGGTACCTCTGCGAGGTTACCTGTGGCACACATGGTGTGCAATCAATCACCACCAGTGGGAAACAAGCCCAGCCTTATGACCTTCCGTGAGGTAGAGACTGTCTTCCATGAATTTGGTCATGCACTTCAGCATATGCTGACTAAGCAGGATGAGGGTCTAGTTGCTGGTATTCGGGGAATAGAATGGGATGCTGTGGAATTACCCTCTCAGTTCATGGAAAATTGGTGCTACCATAGGGATACTTTAATGAGCATTGCAAAACATTATGAAACTGGGGAGACTCTTCCAGAAGAGACATATTTGAAGCTTCTGGCAGCTAGGACTTTCCGTGCAGGCTCCCTGAGCCTTCGGCAGATAAGATTTGCAAGTGTAGATCTGAAGCTGCACACAGATTATGTACCTGGTGGAGCAGAATCCATATGTGATGTTGATTGCAGGGTTAGTGAACGAACACAAGTGATCCCTCCACTTCCAGAAGACAGGTTCCTTTGTGGTTTCAGCCACATATTTGCAGGTGGATATGCAGCTGGGTACTACAGTTACAAGTGGGCTGAGGTGTTGTCTGCCGATGCTTTCTCTGCATTTGAGGATGCGGGATTGGATAACAGCAAGGCTGTTAAAGAGACTGGGAACAAGTTCCGAGAAACCATCCTTGCTCTTGGAGGCGGGAAAGCGCCACTGGAGGTTTTCGTGGAGTTCCGAGGTCGTGAACCTTCACCAGAACCACTGCTCAGGCACAATGGCCTATTAGCCACAGCTTCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031974 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M3

Protein Analysis

801

Amino Acids

89.79

Weight (kDa)

5.83

Isoelectric Point (pI)

49.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP_N	PF19310	131 - 253	2.2e-56	Neurolysin/Thimet oligopeptidase, N-terminal domain
Peptidase_M3	PF01432	333 - 793	5.2e-134	Peptidase family M3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10540 AT5G65620 AT5G65620
fragaria_vesca	FvH4_1g19050 FvH4_1g19050 FvH4_1g19050
malus_domestica	MD01G1044200.v1.1
prunus_persica	Prupe.6G167100_v2.0.a1 Prupe.6G167100_v2.0.a1 Prupe.6G209200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0110061 RchiOBHm_Chr7g0233391
rosa_laevigata	RLG00000001272 RLG00000017783
rosa_multiflora	Rmu_co8163522.1_g000001 Rmu_co8337137.1_g000001 Rmu_sc0008806.1_g000009 Rmu_sc0009935.1_g000003 Rmu_sc0013750.1_g000001 Rmu_sc0024311.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133750
rosa_rugosa	Rorug02G0165400 Rorug02G0165400
rosa_samantha	Rh2AG217200 Rh2BG227200 Rh2CG219400 Rh2DG222900 Rh7CG455700
rosa_wichuraiana	Rw0G010890 Rw2G016780 Rw7G035960 Rw7G035980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2138
AatII	GACGTC	1 cut(s)	363
Acc36I	ACCTGC	1 cut(s)	2138
Acc65I	GGTACC	1 cut(s)	1637
AccB1I	GGYRCC	1 cut(s)	1637
AccB7I	CCANNNNNTGG	1 cut(s)	1329
AccII	CGCG	2 cut(s)	75, 396
AciI	CCGC	9 cut(s)	288, 294, 297, 300, 382, 476, 917, 2228, 2301
AclI	AACGTT	1 cut(s)	145
AclWI	GGATC	2 cut(s)	1547, 2089
AcoI	YGGCCR	1 cut(s)	285
AcsI	RAATTY	5 cut(s)	764, 806, 1267, 1355, 1745
AcuI	CTGAAG	3 cut(s)	1314, 1745, 2033
AcvI	CACGTG	2 cut(s)	456, 1631
AcyI	GRCGYC	2 cut(s)	360, 369
AdeI	CACNNNGTG	1 cut(s)	1669
AfaI	GTAC	4 cut(s)	51, 1639, 2034, 2168
AfiI	CCNNNNNNNGG	6 cut(s)	532, 715, 1329, 1466, 1647, 1691
AflII	CTTAAG	1 cut(s)	1262
AjiI	CACGTC	1 cut(s)	379
AjnI	CCWGG	2 cut(s)	385, 2035
AjuI	GAANNNNNNNTTGG	2 cut(s)	1100, 1132
Alw21I	GWGCWC	2 cut(s)	63, 1206
Alw26I	GTCTC	6 cut(s)	183, 804, 1724, 1907, 1922, 2252
AlwI	GGATC	2 cut(s)	1547, 2089
AlwNI	CAGNNNCTG	2 cut(s)	890, 2396
Ama87I	CYCGRG	1 cut(s)	413
AoxI	GGCC	5 cut(s)	285, 956, 979, 1414, 2377
ApeKI	GCWGC	6 cut(s)	317, 905, 1238, 1949, 2017, 2159
ApoI	RAATTY	5 cut(s)	764, 806, 1267, 1355, 1745
Asp700I	GAANNNNTTC	3 cut(s)	1215, 1225, 2270
Asp718I	GGTACC	1 cut(s)	1637
AspLEI	GCGC	4 cut(s)	398, 441, 1477, 2311
AspS9I	GGNCC	2 cut(s)	956, 980
AsuC2I	CCSGG	1 cut(s)	1019
AsuHPI	GGTGA	3 cut(s)	431, 1674, 2343
AsuII	TTCGAA	1 cut(s)	1219
AvaI	CYCGRG	1 cut(s)	413
BaeI	ACNNNNGTAYC	2 cut(s)	2158, 2191
BanI	GGYRCC	1 cut(s)	1637
BanII	GRGCYC	2 cut(s)	453, 1206
BauI	CACGAG	1 cut(s)	1056
BbrPI	CACGTG	2 cut(s)	456, 1631
BbsI	GAAGAC	2 cut(s)	1729, 2118
Bbv12I	GWGCWC	2 cut(s)	63, 1206
BbvCI	CCTCAGC	2 cut(s)	1155, 2187
BbvI	GCAGC	6 cut(s)	329, 892, 1225, 1961, 2004, 2171
BccI	CCATC	7 cut(s)	244, 1406, 1460, 1576, 1588, 1628, 2291
BceAI	ACGGC	1 cut(s)	994
BcgI	CGANNNNNNTGC	2 cut(s)	992, 1026
BciT130I	CCWGG	2 cut(s)	387, 2037
BciVI	GTATCC	1 cut(s)	1867
BcnI	CCSGG	1 cut(s)	1019
BcoDI	GTCTC	6 cut(s)	183, 804, 1724, 1907, 1922, 2252
BfaI	CTAG	8 cut(s)	479, 788, 1208, 1427, 1472, 1611, 1793, 1953
BfmI	CTRYAG	2 cut(s)	1239, 2170
BfoI	RGCGCY	2 cut(s)	1478, 2312
BfrI	CTTAAG	1 cut(s)	1262
BfuAI	ACCTGC	1 cut(s)	2138
BfuI	GTATCC	1 cut(s)	1867
BglII	AGATCT	3 cut(s)	486, 1258, 2008
Bme1390I	CCNGG	3 cut(s)	387, 1019, 2037
BmeT110I	CYCGRG	1 cut(s)	413
BmgBI	CACGTC	1 cut(s)	379
BmgT120I	GGNCC	2 cut(s)	956, 980
BmiI	GGNNCC	4 cut(s)	450, 1639, 1972, 2120
BmrFI	CCNGG	3 cut(s)	387, 1019, 2037
BmrI	ACTGGG	3 cut(s)	1324, 1917, 2271
BmsI	GCATC	9 cut(s)	673, 817, 1070, 1225, 1276, 1518, 1813, 2194, 2215
BmuI	ACTGGG	3 cut(s)	1324, 1917, 2271
BpiI	GAAGAC	2 cut(s)	1729, 2118
BplI	GAGNNNNNCTC	2 cut(s)	670, 702
BpmI	CTGGAG	1 cut(s)	2336
Bpu10I	CCTNAGC	5 cut(s)	605, 1155, 1976, 2187, 2366
Bpu14I	TTCGAA	1 cut(s)	1219
BpuEI	CTTGAG	2 cut(s)	515, 764
BpuMI	CCSGG	1 cut(s)	1019
BsaAI	YACGTR	2 cut(s)	456, 1631
BsaBI	GATNNNNATC	1 cut(s)	1308
BsaHI	GRCGYC	2 cut(s)	360, 369
BsaI	GGTCTC	1 cut(s)	183
BsaJI	CCNNGG	6 cut(s)	282, 385, 386, 959, 1401, 2335
BsaXI	ACNNNNNCTCC	2 cut(s)	440, 470
Bsc4I	CCNNNNNNNGG	6 cut(s)	532, 715, 1329, 1466, 1647, 1691
Bse118I	RCCGGY	2 cut(s)	1459, 1477
Bse1I	ACTGG	6 cut(s)	1065, 1319, 1688, 1912, 2266, 2319
Bse3DI	GCAATG	2 cut(s)	1533, 1887
Bse8I	GATNNNNATC	1 cut(s)	1308
BseBI	CCWGG	2 cut(s)	387, 2037
BseDI	CCNNGG	6 cut(s)	282, 385, 386, 959, 1401, 2335
BseJI	GATNNNNATC	1 cut(s)	1308
BseLI	CCNNNNNNNGG	6 cut(s)	532, 715, 1329, 1466, 1647, 1691
BseMI	GCAATG	2 cut(s)	1533, 1887
BseMII	CTCAG	8 cut(s)	105, 939, 1146, 1563, 1856, 1967, 2178, 2380
BseNI	ACTGG	6 cut(s)	1065, 1319, 1688, 1912, 2266, 2319
BseRI	GAGGAG	5 cut(s)	108, 253, 320, 538, 1214
BseXI	GCAGC	6 cut(s)	329, 892, 1225, 1961, 2004, 2171
BseYI	CCCAGC	3 cut(s)	372, 1703, 2162
BsgI	GTGCAG	3 cut(s)	891, 1986, 2003
Bsh1236I	CGCG	2 cut(s)	75, 396
BshFI	GGCC	5 cut(s)	287, 958, 981, 1416, 2379
BshNI	GGYRCC	1 cut(s)	1637
BsiHKAI	GWGCWC	2 cut(s)	63, 1206
BsiHKCI	CYCGRG	1 cut(s)	413
BsiSI	CCGG	4 cut(s)	584, 1018, 1460, 1478
BslFI	GGGAC	2 cut(s)	1331, 1515
BslI	CCNNNNNNNGG	6 cut(s)	532, 715, 1329, 1466, 1647, 1691
BsmAI	GTCTC	6 cut(s)	183, 804, 1724, 1907, 1922, 2252
BsmFI	GGGAC	2 cut(s)	1331, 1515
BsnI	GGCC	5 cut(s)	287, 958, 981, 1416, 2379
Bso31I	GGTCTC	1 cut(s)	183
BsoBI	CYCGRG	1 cut(s)	413
Bsp119I	TTCGAA	1 cut(s)	1219
Bsp1286I	GDGCHC	3 cut(s)	63, 453, 1206
Bsp143I	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
Bsp19I	CCATGG	1 cut(s)	282
BspACI	CCGC	9 cut(s)	288, 294, 297, 300, 382, 476, 917, 2228, 2301
BspANI	GGCC	5 cut(s)	287, 958, 981, 1416, 2379
BspCNI	CTCAG	8 cut(s)	104, 940, 1147, 1564, 1855, 1968, 2179, 2379
BspFNI	CGCG	2 cut(s)	75, 396
BspLI	GGNNCC	4 cut(s)	450, 1639, 1972, 2120
BspMAI	CTGCAG	1 cut(s)	1243
BspMI	ACCTGC	1 cut(s)	2138
BspPI	GGATC	2 cut(s)	1547, 2089
BspT104I	TTCGAA	1 cut(s)	1219
BspT107I	GGYRCC	1 cut(s)	1637
BspTI	CTTAAG	1 cut(s)	1262
BspTNI	GGTCTC	1 cut(s)	183
BsrDI	GCAATG	2 cut(s)	1533, 1887
BsrFI	RCCGGY	2 cut(s)	1459, 1477
BsrI	ACTGG	6 cut(s)	1065, 1319, 1688, 1912, 2266, 2319
BssAI	RCCGGY	2 cut(s)	1459, 1477
BssECI	CCNNGG	6 cut(s)	282, 385, 386, 959, 1401, 2335
BssMI	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
BssNI	GRCGYC	2 cut(s)	360, 369
BssSI	CACGAG	1 cut(s)	1056
BssT1I	CCWWGG	3 cut(s)	282, 959, 1401
Bst2BI	CACGAG	1 cut(s)	1056
Bst2UI	CCWGG	2 cut(s)	387, 2037
Bst4CI	ACNGT	6 cut(s)	445, 798, 1043, 1186, 1735, 2174
Bst6I	CTCTTC	3 cut(s)	48, 1920, 1923
BstACI	GRCGYC	2 cut(s)	360, 369
BstAFI	CTTAAG	1 cut(s)	1262
BstBAI	YACGTR	2 cut(s)	456, 1631
BstBI	TTCGAA	1 cut(s)	1219
BstC8I	GCNNGC	4 cut(s)	688, 885, 1461, 1969
BstDSI	CCRYGG	1 cut(s)	282
BstEII	GGTNACC	1 cut(s)	1650
BstENI	CCTNNNNNAGG	1 cut(s)	1645
BstFNI	CGCG	2 cut(s)	75, 396
BstH2I	RGCGCY	2 cut(s)	1478, 2312
BstHHI	GCGC	4 cut(s)	398, 441, 1477, 2311
BstKTI	GATC	5 cut(s)	489, 1261, 1555, 2011, 2097
BstMAI	GTCTC	6 cut(s)	183, 804, 1724, 1907, 1922, 2252
BstMBI	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
BstNI	CCWGG	2 cut(s)	387, 2037
BstNSI	RCATGY	2 cut(s)	1010, 1438
BstPI	GGTNACC	1 cut(s)	1650
BstSCI	CCNGG	3 cut(s)	385, 1017, 2035
BstSFI	CTRYAG	2 cut(s)	1239, 2170
BstUI	CGCG	2 cut(s)	75, 396
BstV1I	GCAGC	6 cut(s)	329, 892, 1225, 1961, 2004, 2171
BstV2I	GAAGAC	2 cut(s)	1729, 2118
BstX2I	RGATCY	3 cut(s)	486, 1258, 2008
BstXI	CCANNNNNNTGG	4 cut(s)	283, 895, 1408, 1748
BstYI	RGATCY	3 cut(s)	486, 1258, 2008
BsuI	GTATCC	1 cut(s)	1867
BsuRI	GGCC	5 cut(s)	287, 958, 981, 1416, 2379
BtgI	CCRYGG	1 cut(s)	282
BtrI	CACGTC	1 cut(s)	379
BtsI	GCAGTG	2 cut(s)	888, 2360
BtsIMutI	CAGTG	6 cut(s)	888, 1072, 1312, 1695, 2312, 2360
BveI	ACCTGC	1 cut(s)	2138
Cac8I	GCNNGC	4 cut(s)	688, 885, 1461, 1969
CaiI	CAGNNNCTG	2 cut(s)	890, 2396
CfoI	GCGC	4 cut(s)	398, 441, 1477, 2311
Cfr10I	RCCGGY	2 cut(s)	1459, 1477
Cfr13I	GGNCC	2 cut(s)	956, 980
CseI	GACGC	3 cut(s)	64, 377, 1370
CsiI	ACCWGGT	1 cut(s)	2035
Csp6I	GTAC	4 cut(s)	50, 1638, 2033, 2167
CviQI	GTAC	4 cut(s)	50, 1638, 2033, 2167
DpnI	GATC	5 cut(s)	488, 1260, 1554, 2010, 2096
DpnII	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
DraI	TTTAAA	1 cut(s)	643
DraIII	CACNNNGTG	1 cut(s)	1669
EaeI	YGGCCR	1 cut(s)	285
Eam1104I	CTCTTC	3 cut(s)	48, 1920, 1923
EarI	CTCTTC	3 cut(s)	48, 1920, 1923
EciI	GGCGGA	2 cut(s)	283, 371
Ecl136II	GAGCTC	1 cut(s)	1204
Eco130I	CCWWGG	3 cut(s)	282, 959, 1401
Eco24I	GRGCYC	2 cut(s)	453, 1206
Eco31I	GGTCTC	1 cut(s)	183
Eco53kI	GAGCTC	1 cut(s)	1204
Eco57I	CTGAAG	3 cut(s)	1314, 1745, 2033
Eco72I	CACGTG	2 cut(s)	456, 1631
Eco88I	CYCGRG	1 cut(s)	413
Eco91I	GGTNACC	1 cut(s)	1650
EcoICRI	GAGCTC	1 cut(s)	1204
EcoNI	CCTNNNNNAGG	1 cut(s)	1645
EcoO65I	GGTNACC	1 cut(s)	1650
EcoRI	GAATTC	1 cut(s)	764
EcoRII	CCWGG	2 cut(s)	385, 2035
EcoT14I	CCWWGG	3 cut(s)	282, 959, 1401
EcoT22I	ATGCAT	1 cut(s)	640
EcoT38I	GRGCYC	2 cut(s)	453, 1206
ErhI	CCWWGG	3 cut(s)	282, 959, 1401
FalI	AAGNNNNNCTT	2 cut(s)	1116, 1148
FaqI	GGGAC	2 cut(s)	1331, 1515
FauI	CCCGC	2 cut(s)	2221, 2294
FauNDI	CATATG	2 cut(s)	1767, 2054
FriOI	GRGCYC	2 cut(s)	453, 1206
FspBI	CTAG	8 cut(s)	479, 788, 1208, 1427, 1472, 1611, 1793, 1953
GlaI	GCGC	4 cut(s)	397, 440, 1476, 2310
GsaI	CCCAGC	3 cut(s)	376, 1707, 2166
GsuI	CTGGAG	1 cut(s)	2336
HaeII	RGCGCY	2 cut(s)	1478, 2312
HaeIII	GGCC	5 cut(s)	287, 958, 981, 1416, 2379
HapII	CCGG	4 cut(s)	584, 1018, 1460, 1478
HgaI	GACGC	3 cut(s)	64, 377, 1370
HhaI	GCGC	4 cut(s)	398, 441, 1477, 2311
Hin1I	GRCGYC	2 cut(s)	360, 369
Hin6I	GCGC	4 cut(s)	396, 439, 1475, 2309
HinP1I	GCGC	4 cut(s)	396, 439, 1475, 2309
HincII	GTYRAC	1 cut(s)	523
HindII	GTYRAC	1 cut(s)	523
HindIII	AAGCTT	2 cut(s)	1214, 1940
HpaII	CCGG	4 cut(s)	584, 1018, 1460, 1478
HphI	GGTGA	3 cut(s)	431, 1674, 2343
Hpy166II	GTNNAC	6 cut(s)	97, 459, 523, 623, 2081, 2345
Hpy188I	TCNGA	7 cut(s)	553, 814, 1137, 1573, 2013, 2276, 2336
Hpy8I	GTNNAC	6 cut(s)	97, 459, 523, 623, 2081, 2345
Hpy99I	CGWCG	2 cut(s)	362, 371
HpyAV	CCTTC	6 cut(s)	202, 364, 869, 1726, 1991, 2358
HpyCH4III	ACNGT	6 cut(s)	445, 798, 1043, 1186, 1735, 2174
HpyCH4IV	ACGT	8 cut(s)	57, 145, 360, 378, 455, 559, 694, 1630
HpySE526I	ACGT	8 cut(s)	57, 145, 360, 378, 455, 559, 694, 1630
Hsp92I	GRCGYC	2 cut(s)	360, 369
HspAI	GCGC	4 cut(s)	396, 439, 1475, 2309
KpnI	GGTACC	1 cut(s)	1641
KroI	GCCGGC	1 cut(s)	1459
KroNI	GCCGGC	1 cut(s)	1461
Kzo9I	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
LmnI	GCTCC	7 cut(s)	266, 448, 1201, 1209, 1294, 1976, 2042
Lsp1109I	GCAGC	6 cut(s)	329, 892, 1225, 1961, 2004, 2171
LweI	GCATC	9 cut(s)	673, 817, 1070, 1225, 1276, 1518, 1813, 2194, 2215
MabI	ACCWGGT	1 cut(s)	2035
MaeI	CTAG	8 cut(s)	479, 788, 1208, 1427, 1472, 1611, 1793, 1953
MaeII	ACGT	8 cut(s)	57, 145, 360, 378, 455, 559, 694, 1630
MaeIII	GTNAC	5 cut(s)	240, 1495, 1626, 1650, 2174
MalI	GATC	5 cut(s)	488, 1260, 1554, 2010, 2096
MboI	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
MflI	RGATCY	3 cut(s)	486, 1258, 2008
MhlI	GDGCHC	3 cut(s)	63, 453, 1206
MlyI	GAGTC	8 cut(s)	36, 539, 1091, 1288, 1358, 1537, 1624, 1909
MmeI	TCCRAC	1 cut(s)	1098
Mph1103I	ATGCAT	1 cut(s)	640
MroNI	GCCGGC	1 cut(s)	1459
MroXI	GAANNNNTTC	3 cut(s)	1215, 1225, 2270
MseI	TTAA	8 cut(s)	108, 540, 594, 642, 851, 1263, 1880, 2253
MslI	CAYNNNNRTG	2 cut(s)	281, 936
MspA1I	CMGCKG	2 cut(s)	917, 2162
MspCI	CTTAAG	1 cut(s)	1262
MspI	CCGG	4 cut(s)	584, 1018, 1460, 1478
MspR9I	CCNGG	3 cut(s)	387, 1019, 2037
MvaI	CCWGG	2 cut(s)	387, 2037
MvnI	CGCG	2 cut(s)	75, 396
NaeI	GCCGGC	1 cut(s)	1461
NciI	CCSGG	1 cut(s)	1019
NcoI	CCATGG	1 cut(s)	282
NdeI	CATATG	2 cut(s)	1767, 2054
NdeII	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
NgoMIV	GCCGGC	1 cut(s)	1459
NlaIV	GGNNCC	4 cut(s)	450, 1639, 1972, 2120
NmuCI	GTSAC	2 cut(s)	240, 1626
NsiI	ATGCAT	1 cut(s)	640
NspI	RCATGY	2 cut(s)	1010, 1438
NspV	TTCGAA	1 cut(s)	1219
PaeR7I	CTCGAG	1 cut(s)	413
PaqCI	CACCTGC	1 cut(s)	2138
PasI	CCCWGGG	1 cut(s)	386
PcsI	WCGNNNNNNNCGW	2 cut(s)	363, 375
PdiI	GCCGGC	1 cut(s)	1461
PdmI	GAANNNNTTC	3 cut(s)	1215, 1225, 2270
PfeI	GAWTC	5 cut(s)	391, 656, 1309, 1517, 2048
PflMI	CCANNNNNTGG	1 cut(s)	1329
PleI	GAGTC	8 cut(s)	35, 539, 1091, 1287, 1357, 1536, 1623, 1909
PmaCI	CACGTG	2 cut(s)	456, 1631
PmlI	CACGTG	2 cut(s)	456, 1631
PpsI	GAGTC	8 cut(s)	35, 539, 1091, 1287, 1357, 1536, 1623, 1909
Ppu21I	YACGTR	2 cut(s)	456, 1631
Psp124BI	GAGCTC	1 cut(s)	1206
Psp1406I	AACGTT	1 cut(s)	145
Psp6I	CCWGG	2 cut(s)	385, 2035
PspCI	CACGTG	2 cut(s)	456, 1631
PspEI	GGTNACC	1 cut(s)	1650
PspFI	CCCAGC	3 cut(s)	372, 1703, 2162
PspGI	CCWGG	2 cut(s)	385, 2035
PspN4I	GGNNCC	4 cut(s)	450, 1639, 1972, 2120
PspPI	GGNCC	2 cut(s)	956, 980
PspXI	VCTCGAGB	1 cut(s)	413
PstI	CTGCAG	1 cut(s)	1243
PstNI	CAGNNNCTG	2 cut(s)	890, 2396
PsuI	RGATCY	3 cut(s)	486, 1258, 2008
PvuII	CAGCTG	1 cut(s)	2162
RsaI	GTAC	4 cut(s)	51, 1639, 2034, 2168
RsaNI	GTAC	4 cut(s)	50, 1638, 2033, 2167
RseI	CAYNNNNRTG	2 cut(s)	281, 936
SacI	GAGCTC	1 cut(s)	1206
SaqAI	TTAA	8 cut(s)	108, 540, 594, 642, 851, 1263, 1880, 2253
Sau3AI	GATC	5 cut(s)	486, 1258, 1552, 2008, 2094
Sau96I	GGNCC	2 cut(s)	956, 980
SchI	GAGTC	8 cut(s)	36, 539, 1091, 1288, 1358, 1537, 1624, 1909
ScrFI	CCNGG	3 cut(s)	387, 1019, 2037
SduI	GDGCHC	3 cut(s)	63, 453, 1206
SexAI	ACCWGGT	1 cut(s)	2035
SfaNI	GCATC	9 cut(s)	673, 817, 1070, 1225, 1276, 1518, 1813, 2194, 2215
SfcI	CTRYAG	2 cut(s)	1239, 2170
Sfr274I	CTCGAG	1 cut(s)	413
SfuI	TTCGAA	1 cut(s)	1219
SlaI	CTCGAG	1 cut(s)	413
SmiMI	CAYNNNNRTG	2 cut(s)	281, 936
SmlI	CTYRAG	4 cut(s)	413, 530, 743, 1262
SmoI	CTYRAG	4 cut(s)	413, 530, 743, 1262
SsiI	CCGC	9 cut(s)	288, 294, 297, 300, 382, 476, 917, 2228, 2301
SspMI	CTAG	8 cut(s)	479, 788, 1208, 1427, 1472, 1611, 1793, 1953
SstI	GAGCTC	1 cut(s)	1206
StyD4I	CCNGG	3 cut(s)	385, 1017, 2035
StyI	CCWWGG	3 cut(s)	282, 959, 1401
TaaI	ACNGT	6 cut(s)	445, 798, 1043, 1186, 1735, 2174
TaiI	ACGT	8 cut(s)	60, 148, 363, 381, 458, 562, 697, 1633
TaqI	TCGA	7 cut(s)	357, 366, 414, 652, 1012, 1100, 1219
TaqII	GACCGA	1 cut(s)	510
TatI	WGTACW	1 cut(s)	49
TauI	GCSGC	4 cut(s)	290, 299, 302, 478
TfiI	GAWTC	5 cut(s)	391, 656, 1309, 1517, 2048
Tru1I	TTAA	8 cut(s)	108, 540, 594, 642, 851, 1263, 1880, 2253
Tru9I	TTAA	8 cut(s)	108, 540, 594, 642, 851, 1263, 1880, 2253
TscAI	CASTG	6 cut(s)	895, 1072, 1319, 1695, 2319, 2367
TseFI	GTSAC	2 cut(s)	240, 1626
TseI	GCWGC	6 cut(s)	317, 905, 1238, 1949, 2017, 2159
Tsp45I	GTSAC	2 cut(s)	240, 1626
TspDTI	ATGAA	9 cut(s)	17, 777, 882, 948, 1218, 1614, 1758, 1837, 1917
TspGWI	ACGGA	2 cut(s)	1709, 1952
TspRI	CASTG	6 cut(s)	895, 1072, 1319, 1695, 2319, 2367
Van91I	CCANNNNNTGG	1 cut(s)	1329
Vha464I	CTTAAG	1 cut(s)	1262
XagI	CCTNNNNNAGG	1 cut(s)	1645
XapI	RAATTY	5 cut(s)	764, 806, 1267, 1355, 1745
XceI	RCATGY	2 cut(s)	1010, 1438
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1072
XhoI	CTCGAG	1 cut(s)	413
XmnI	GAANNNNTTC	3 cut(s)	1215, 1225, 2270
XspI	CTAG	8 cut(s)	479, 788, 1208, 1427, 1472, 1611, 1793, 1953
ZraI	GACGTC	1 cut(s)	361
Zsp2I	ATGCAT	1 cut(s)	640

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.