AT5G66005

NTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002366
Physical Location & Seq
Reverse (-)
26397519 .. 26399274
1756 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66005.2

Sequence Viewer

Length: 444 bp
ATGAGGGAACGAGGACAAAGGGTTGGTTTTCAAGTGGTCACTTTGGATGGCCGCACAAGTTTGCTTGCTTCTTCTATTGTTTCTAGTCAAGAATCGATGACATGGCCTAGTGTTGGCAAGTACAAAGTGGACATTGCATCCTTCGAGTCTATAGCCTTGCCTGAGCTACAGGTCAAAGACGATACCAATCTTTTCATCATTGATGAAGTTGGCAAGATGGAGATGTTTAGTCCATCCTTTTTTCCGGCTGTCCTCAATGTTTTGGACTCCAACGTTCCTCTTCTTGCTTCCATTCCTAGCCCAAAATTTGGTCGACATCTTCCTGAAGTGGCAAGGTTGAAGAACCAACCGGGAGTGAATGTAATCTCCCTGAGTGCGACTAACAGAGATCCCATGAAAGAACATATCTTTGATGTTTTCTCTGGTTGGCTGCCCAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 map00230 map00730 map01100
Pfam Domains
Protein Families
NTPase_1

Protein Analysis

147

Amino Acids

16.32

Weight (kDa)

6.1

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTPase_1 PF03266 8 - 146 4.7e-36 NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 308
AccI GTMKAC 1 cut(s) 313
AciI CCGC 1 cut(s) 52
AclI AACGTT 1 cut(s) 273
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 2 cut(s) 113, 308
AgsI TTSAA 2 cut(s) 32, 340
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
AlwI GGATC 1 cut(s) 383
AoxI GGCC 2 cut(s) 49, 104
ApeKI GCWGC 1 cut(s) 430
ApoI RAATTY 1 cut(s) 305
AsuC2I CCSGG 1 cut(s) 351
BbvI GCAGC 1 cut(s) 417
BccI CCATC 3 cut(s) 41, 211, 241
BcnI CCSGG 1 cut(s) 351
BfaI CTAG 3 cut(s) 84, 108, 297
BfmI CTRYAG 2 cut(s) 150, 167
BisI GCNGC 2 cut(s) 52, 431
BlsI GCNGC 2 cut(s) 53, 432
Bme1390I CCNGG 1 cut(s) 351
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 351
Bsa29I ATCGAT 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 308
Bse3DI GCAATG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 186
BseCI ATCGAT 1 cut(s) 95
BseGI GGATG 3 cut(s) 52, 137, 233
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 2 cut(s) 113, 308
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 2 cut(s) 153, 362
BseXI GCAGC 1 cut(s) 417
BshFI GGCC 2 cut(s) 51, 106
BshVI ATCGAT 1 cut(s) 95
BsiSI CCGG 2 cut(s) 245, 350
BslI CCNNNNNNNGG 2 cut(s) 113, 308
BsnI GGCC 2 cut(s) 51, 106
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 1 cut(s) 52
BspANI GGCC 2 cut(s) 51, 106
BspCNI CTCAG 2 cut(s) 154, 363
BspDI ATCGAT 1 cut(s) 95
BspPI GGATC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 132
BssMI GATC 1 cut(s) 388
Bst6I CTCTTC 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 162, 371
BstF5I GGATG 3 cut(s) 52, 137, 233
BstKTI GATC 1 cut(s) 391
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 349
BstSFI CTRYAG 2 cut(s) 150, 167
BstV1I GCAGC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
Bsu15I ATCGAT 1 cut(s) 95
BsuRI GGCC 2 cut(s) 51, 106
BsuTUI ATCGAT 1 cut(s) 95
BtsCI GGATG 3 cut(s) 52, 137, 233
Cac8I GCNNGC 1 cut(s) 66
ClaI ATCGAT 1 cut(s) 95
Csp6I GTAC 1 cut(s) 121
CviAII CATG 2 cut(s) 102, 394
CviJI RGCY 7 cut(s) 51, 106, 155, 166, 248, 300, 430
CviKI_1 RGCY 7 cut(s) 51, 106, 155, 166, 248, 300, 430
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 2 cut(s) 162, 371
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 345
FaeI CATG 2 cut(s) 105, 397
FaiI YATR 4 cut(s) 103, 152, 395, 405
FatI CATG 2 cut(s) 101, 393
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 52, 431
FokI GGATG 3 cut(s) 59, 124, 220
Fsp4HI GCNGC 2 cut(s) 52, 431
FspBI CTAG 3 cut(s) 84, 108, 297
GluI GCNGC 2 cut(s) 52, 431
HaeIII GGCC 2 cut(s) 51, 106
HapII CCGG 2 cut(s) 245, 350
Hin1II CATG 2 cut(s) 105, 397
HincII GTYRAC 1 cut(s) 314
HindII GTYRAC 1 cut(s) 314
HinfI GANTC 3 cut(s) 92, 146, 266
HpaII CCGG 2 cut(s) 245, 350
Hpy166II GTNNAC 2 cut(s) 130, 314
Hpy188III TCNNGA 2 cut(s) 89, 323
Hpy8I GTNNAC 2 cut(s) 130, 314
HpyAV CCTTC 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 436
HpyF3I CTNAG 2 cut(s) 162, 371
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 2 cut(s) 105, 397
Kzo9I GATC 1 cut(s) 388
LpnPI CCDG 7 cut(s) 155, 174, 258, 336, 363, 383, 408
Lsp1109I GCAGC 1 cut(s) 417
LweI GCATC 1 cut(s) 146
MaeI CTAG 3 cut(s) 84, 108, 297
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 37
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 4 cut(s) 63, 272, 311, 352
MflI RGATCY 1 cut(s) 388
MluCI AATT 1 cut(s) 305
MlyI GAGTC 2 cut(s) 155, 260
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 3 cut(s) 5, 263, 288
MspI CCGG 2 cut(s) 245, 350
MspR9I CCNGG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 436
NciI CCSGG 1 cut(s) 351
NdeII GATC 1 cut(s) 388
NlaIII CATG 2 cut(s) 105, 397
NmuCI GTSAC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 2 cut(s) 53, 432
PleI GAGTC 2 cut(s) 154, 260
PpsI GAGTC 2 cut(s) 154, 260
Psp1406I AACGTT 1 cut(s) 273
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SalI GTCGAC 1 cut(s) 312
SatI GCNGC 2 cut(s) 52, 431
Sau3AI GATC 1 cut(s) 388
SchI GAGTC 2 cut(s) 155, 260
ScrFI CCNGG 1 cut(s) 351
SetI ASST 4 cut(s) 168, 174, 276, 338
SfaNI GCATC 1 cut(s) 146
SfcI CTRYAG 2 cut(s) 150, 167
Sse9I AATT 1 cut(s) 305
SsiI CCGC 1 cut(s) 52
SspMI CTAG 3 cut(s) 84, 108, 297
StyD4I CCNGG 1 cut(s) 349
TaiI ACGT 1 cut(s) 276
TaqI TCGA 3 cut(s) 95, 144, 313
TasI AATT 1 cut(s) 305
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 1 cut(s) 92
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 184, 219, 410
Van91I CCANNNNNTGG 1 cut(s) 308
XapI RAATTY 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 313
XspI CTAG 3 cut(s) 84, 108, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.