MD15G1231400.v1.1

Cancer-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002366
Physical Location & Seq
Reverse (-)
18934241 .. 18934914
674 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1231400.v1.1.491

Sequence Viewer

Length: 282 bp
ATGGCCGGCCCCGGAAAGTGCTTGCTGGTGACTGGACCTCCTGGTGTGGGCAAAAGCACTTTAGTAATGAGAGTGTTTGAAACCCTAAAAGCATCATATCCAAACTTAAAAATTCAGGGTTTCTACACTAGTGAGGTTAGAGAAGGAAGTGAGAGAGTGGGGTTCCAGGTGGTCACGTTGGACGGCCGTACAGCTCCCCTCTCATCCTCCACCATTTCCAGGTCTTCCTTTTCATCCATTCTAAATGTTTGTTGCTTCAGTTTCCATAACTCCATTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 map00230 map00730 map01100
Pfam Domains
Protein Families
NTPase_1

Protein Analysis

94

Amino Acids

9.94

Weight (kDa)

9.3

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 5 - 32 2.4e-06 AAA ATPase domain
NTPase_1 PF03266 8 - 75 1.6e-19 NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 3, 184
AcsI RAATTY 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 241
AfaI GTAC 1 cut(s) 190
AfiI CCNNNNNNNGG 2 cut(s) 47, 219
AgsI TTSAA 1 cut(s) 80
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 3 cut(s) 40, 165, 218
AluBI AGCT 1 cut(s) 194
AluI AGCT 1 cut(s) 194
AoxI GGCC 3 cut(s) 3, 7, 184
ApoI RAATTY 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 8, 35
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 216
BceAI ACGGC 2 cut(s) 171, 199
BciT130I CCWGG 3 cut(s) 42, 167, 220
BcnI CCSGG 1 cut(s) 12
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 2 cut(s) 129, 280
Bme1390I CCNGG 4 cut(s) 12, 42, 167, 220
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 2 cut(s) 8, 35
BmiI GGNNCC 2 cut(s) 10, 164
BmrFI CCNGG 4 cut(s) 12, 42, 167, 220
BmsI GCATC 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 10
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 219
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 37
BseBI CCWGG 3 cut(s) 42, 167, 220
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 2 cut(s) 203, 233
BseLI CCNNNNNNNGG 2 cut(s) 47, 219
BseNI ACTGG 1 cut(s) 37
BseX3I CGGCCG 1 cut(s) 184
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 3 cut(s) 5, 9, 186
BsiEI CGRYCG 1 cut(s) 187
BsiSI CCGG 2 cut(s) 6, 12
BslI CCNNNNNNNGG 2 cut(s) 47, 219
BsnI GGCC 3 cut(s) 5, 9, 186
BspANI GGCC 3 cut(s) 5, 9, 186
BspLI GGNNCC 2 cut(s) 10, 164
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 1 cut(s) 37
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 10
Bst2UI CCWGG 3 cut(s) 42, 167, 220
BstC8I GCNNGC 2 cut(s) 7, 23
BstF5I GGATG 2 cut(s) 203, 233
BstMCI CGRYCG 1 cut(s) 187
BstNI CCWGG 3 cut(s) 42, 167, 220
BstSCI CCNGG 4 cut(s) 10, 40, 165, 218
BstV2I GAAGAC 1 cut(s) 216
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 3 cut(s) 5, 9, 186
BtsCI GGATG 2 cut(s) 203, 233
Cac8I GCNNGC 2 cut(s) 7, 23
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 2 cut(s) 8, 35
Csp6I GTAC 1 cut(s) 189
CviJI RGCY 4 cut(s) 5, 9, 186, 194
CviKI_1 RGCY 4 cut(s) 5, 9, 186, 194
CviQI GTAC 1 cut(s) 189
EaeI YGGCCR 2 cut(s) 3, 184
EagI CGGCCG 1 cut(s) 184
EclXI CGGCCG 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 35
Eco52I CGGCCG 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 241
EcoRII CCWGG 3 cut(s) 40, 165, 218
FaiI YATR 2 cut(s) 97, 267
FokI GGATG 2 cut(s) 190, 220
FseI GGCCGGCC 1 cut(s) 9
FspBI CTAG 2 cut(s) 129, 280
HaeIII GGCC 3 cut(s) 5, 9, 186
HapII CCGG 2 cut(s) 6, 12
HpaII CCGG 2 cut(s) 6, 12
HphI GGTGA 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 137
HpyCH4IV ACGT 1 cut(s) 176
HpySE526I ACGT 1 cut(s) 176
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
LmnI GCTCC 1 cut(s) 199
LweI GCATC 1 cut(s) 101
MaeI CTAG 2 cut(s) 129, 280
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 2 cut(s) 28, 172
MboII GAAGA 1 cut(s) 216
MluCI AATT 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 4 cut(s) 48, 127, 209, 217
MroNI GCCGGC 1 cut(s) 5
MseI TTAA 1 cut(s) 107
MspI CCGG 2 cut(s) 6, 12
MspR9I CCNGG 4 cut(s) 12, 42, 167, 220
MvaI CCWGG 3 cut(s) 42, 167, 220
NaeI GCCGGC 1 cut(s) 7
NciI CCSGG 1 cut(s) 12
NgoMIV GCCGGC 1 cut(s) 5
NlaIV GGNNCC 2 cut(s) 10, 164
NmuCI GTSAC 2 cut(s) 28, 172
PdiI GCCGGC 1 cut(s) 7
Psp6I CCWGG 3 cut(s) 40, 165, 218
PspGI CCWGG 3 cut(s) 40, 165, 218
PspN4I GGNNCC 2 cut(s) 10, 164
PspPI GGNCC 2 cut(s) 8, 35
RigI GGCCGGCC 1 cut(s) 9
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 107
Sau96I GGNCC 2 cut(s) 8, 35
ScrFI CCNGG 4 cut(s) 12, 42, 167, 220
SetI ASST 6 cut(s) 40, 138, 171, 179, 196, 224
SfaNI GCATC 1 cut(s) 101
SinI GGWCC 1 cut(s) 35
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 1 cut(s) 111
SspMI CTAG 2 cut(s) 129, 280
StyD4I CCNGG 4 cut(s) 10, 40, 165, 218
TaiI ACGT 1 cut(s) 179
TasI AATT 1 cut(s) 111
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TseFI GTSAC 2 cut(s) 28, 172
Tsp45I GTSAC 2 cut(s) 28, 172
TspDTI ATGAA 1 cut(s) 222
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 1 cut(s) 111
XspI CTAG 2 cut(s) 129, 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.