MD15G1231300.v1.1

Cancer-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002366
Physical Location & Seq
Reverse (-)
18933028 .. 18934239
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1231300.v1.1.491

Sequence Viewer

Length: 384 bp
ATGCGGGGTTCTACTGTACTAATTAACCCAGAGTCTTTGAGATGGCCTACCGTGGGGAAGTACAAAGTAGATGTTGCGTCGTTTGAGTCACTGGCACTGCCTGAATTGCAGGTAAGAGAAGATACCGATCTATTCATCGTTGATGAAGTTGGGAAGATGGAGTTGTTCAGTTCATCGTTCTTCCCTTGTGTTTTAAGGATTCTGGAGTCCAATGTCCCGTTTTTGGCCACAGTCCCAATTCCAAAATTCGGTCGTGATATACCTGCAGTTGCAAGGTTGAAGAATCATCCGGGGGCAACAATGTTTACGCTGAGCAAAGGTAACAGGGATGCTGTTAAAGAAGAGATATACTCCCATTTGGTAGCTTTGTTGAGTAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 map00230 map00730 map01100
Pfam Domains
Protein Families
NTPase_1

Protein Analysis

128

Amino Acids

14.18

Weight (kDa)

6.73

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTPase_1 PF03266 11 - 116 1.1e-23 NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 100, 271
AciI CCGC 1 cut(s) 4
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 1 cut(s) 245
AfaI GTAC 2 cut(s) 18, 62
AfiI CCNNNNNNNGG 3 cut(s) 53, 223, 248
AgsI TTSAA 1 cut(s) 280
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
AoxI GGCC 2 cut(s) 44, 225
ApoI RAATTY 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 291
BalI TGGCCA 1 cut(s) 227
BccI CCATC 2 cut(s) 36, 151
BcnI CCSGG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 264
BfuAI ACCTGC 2 cut(s) 100, 271
BlpI GCTNAGC 1 cut(s) 311
Bme1390I CCNGG 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 319
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 51, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 223, 248
Bse1I ACTGG 1 cut(s) 96
Bse8I GATNNNNATC 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 51, 290
BseGI GGATG 2 cut(s) 286, 334
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 3 cut(s) 53, 223, 248
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 2 cut(s) 46, 227
BsiEI CGRYCG 1 cut(s) 253
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 2 cut(s) 200, 218
BslI CCNNNNNNNGG 3 cut(s) 53, 223, 248
BsmFI GGGAC 2 cut(s) 200, 218
BsnI GGCC 2 cut(s) 46, 227
Bsp143I GATC 1 cut(s) 127
Bsp1720I GCTNAGC 1 cut(s) 311
BspACI CCGC 1 cut(s) 4
BspANI GGCC 2 cut(s) 46, 227
BspCNI CTCAG 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 268
BspMI ACCTGC 2 cut(s) 100, 271
BsrI ACTGG 1 cut(s) 96
BssECI CCNNGG 2 cut(s) 51, 290
BssMI GATC 1 cut(s) 127
Bst4CI ACNGT 3 cut(s) 16, 52, 232
Bst6I CTCTTC 1 cut(s) 336
BstDEI CTNAG 1 cut(s) 311
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 286, 334
BstKTI GATC 1 cut(s) 130
BstMBI GATC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 264
BsuRI GGCC 2 cut(s) 46, 227
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 286, 334
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 89, 95
BveI ACCTGC 2 cut(s) 100, 271
CseI GACGC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 17, 61
CviJI RGCY 3 cut(s) 46, 227, 365
CviKI_1 RGCY 3 cut(s) 46, 227, 365
CviQI GTAC 2 cut(s) 17, 61
DdeI CTNAG 1 cut(s) 311
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
FaiI YATR 2 cut(s) 260, 349
FaqI GGGAC 2 cut(s) 200, 218
FokI GGATG 2 cut(s) 273, 341
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 2 cut(s) 46, 227
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 66
HinfI GANTC 5 cut(s) 32, 86, 199, 206, 283
HpaII CCGG 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 306, 377
Hpy188III TCNNGA 2 cut(s) 203, 254
Hpy8I GTNNAC 2 cut(s) 306, 377
Hpy99I CGWCG 1 cut(s) 82
HpyCH4III ACNGT 3 cut(s) 16, 52, 232
HpyCH4V TGCA 3 cut(s) 109, 266, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 1 cut(s) 311
Kzo9I GATC 1 cut(s) 127
LpnPI CCDG 8 cut(s) 42, 77, 95, 114, 188, 276, 303, 310
LweI GCATC 1 cut(s) 319
MaeIII GTNAC 2 cut(s) 87, 320
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 5 cut(s) 131, 166, 172, 292, 353
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 4 cut(s) 21, 104, 237, 245
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 3 cut(s) 41, 95, 215
Mox20I TGGCCA 1 cut(s) 227
MscI TGGCCA 1 cut(s) 227
MseI TTAA 3 cut(s) 24, 194, 336
Msp20I TGGCCA 1 cut(s) 227
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 106
NciI CCSGG 1 cut(s) 291
NdeII GATC 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 87
PfeI GAWTC 2 cut(s) 199, 283
PleI GAGTC 3 cut(s) 40, 94, 214
PpsI GAGTC 3 cut(s) 40, 94, 214
PstI CTGCAG 1 cut(s) 268
RsaI GTAC 2 cut(s) 18, 62
RsaNI GTAC 2 cut(s) 17, 61
SaqAI TTAA 3 cut(s) 24, 194, 336
Sau3AI GATC 1 cut(s) 127
SchI GAGTC 3 cut(s) 41, 95, 215
ScrFI CCNGG 1 cut(s) 291
SetI ASST 5 cut(s) 114, 265, 278, 322, 367
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 264
Sse9I AATT 4 cut(s) 21, 104, 237, 245
SsiI CCGC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 16, 52, 232
TaqII GACCGA 1 cut(s) 239
TasI AATT 4 cut(s) 21, 104, 237, 245
TatI WGTACW 2 cut(s) 16, 60
TfiI GAWTC 2 cut(s) 199, 283
Tru1I TTAA 3 cut(s) 24, 194, 336
Tru9I TTAA 3 cut(s) 24, 194, 336
TscAI CASTG 2 cut(s) 96, 102
TseFI GTSAC 1 cut(s) 87
Tsp45I GTSAC 1 cut(s) 87
TspDTI ATGAA 3 cut(s) 124, 159, 162
TspRI CASTG 2 cut(s) 96, 102
XapI RAATTY 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.