Rh5DG335100

Cancer-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002366
Physical Location & Seq
Forward (+)
44594822 .. 44626666
31845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG335100.1

Sequence Viewer

Length: 420 bp
ATGAATGTCGAGGCTGGTTCTGGTGGCAATGCTTTATCTTCTGAGCTCATGGATCATGGTCGTTGCCAGCATGGTGGTGGTGAGGTTAGACAAGGAGGTGAGAGAGTTGGGTTCCAGGTGGTCACATTTGACGGCCGTACTGCTCCACTTGCCTCATCCATCAGTTCCAGCCCTGAATCTTTGAGATGGCCCACTGTGGGGAAGTACAAAGTAGATGTTGCGTCGTTTGAGTCCTTGGCAGTTCCTGAATTACAGGTAAGAGAAGATACTGACCTATTTGTCATCGTTGAAGTCGATAAGATGGAGTTGTTTGGTTCATCTTACTTCCCTGCTGTCTTAAGAGTGCTAGAATCCAATGTTCCAGTCTTGGCGTCAGTTCCAATTCCAAAAGTTGGCCGCGATATACCAGCAGGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00730 ko01100 map00230 map00730 map01100
Pfam Domains
Protein Families
NTPase_1

Protein Analysis

139

Amino Acids

14.79

Weight (kDa)

4.85

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTPase_1 PF03266 28 - 129 2e-17 NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
Acc36I ACCTGC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 392
AccII CGCG 1 cut(s) 399
AciI CCGC 1 cut(s) 397
AclWI GGATC 1 cut(s) 60
AcoI YGGCCR 2 cut(s) 133, 394
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 3 cut(s) 139, 206, 415
AfiI CCNNNNNNNGG 3 cut(s) 197, 198, 392
AflII CTTAAG 1 cut(s) 337
AgsI TTSAA 1 cut(s) 290
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
Alw21I GWGCWC 1 cut(s) 48
AlwI GGATC 1 cut(s) 60
AlwNI CAGNNNCTG 1 cut(s) 245
AoxI GGCC 3 cut(s) 133, 188, 394
AspS9I GGNCC 1 cut(s) 189
AsuHPI GGTGA 2 cut(s) 92, 110
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 48
BccI CCATC 3 cut(s) 167, 180, 295
BceAI ACGGC 2 cut(s) 120, 148
BciT130I CCWGG 1 cut(s) 116
BfaI CTAG 1 cut(s) 347
BfrI CTTAAG 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 401
BisI GCNGC 1 cut(s) 397
BlsI GCNGC 1 cut(s) 398
Bme1390I CCNGG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 234
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 198, 392
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 1 cut(s) 155
BseLI CCNNNNNNNGG 3 cut(s) 197, 198, 392
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 362
BseX3I CGGCCG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 3 cut(s) 135, 190, 396
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 48
BslI CCNNNNNNNGG 3 cut(s) 197, 198, 392
BsnI GGCC 3 cut(s) 135, 190, 396
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 1 cut(s) 52
BspACI CCGC 1 cut(s) 397
BspANI GGCC 3 cut(s) 135, 190, 396
BspCNI CTCAG 1 cut(s) 34
BspFNI CGCG 1 cut(s) 399
BspLI GGNNCC 1 cut(s) 113
BspMI ACCTGC 1 cut(s) 401
BspPI GGATC 1 cut(s) 60
BspTI CTTAAG 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 34
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 1 cut(s) 52
BssNI GRCGYC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 196
BstACI GRCGYC 1 cut(s) 371
BstAFI CTTAAG 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 42
BstF5I GGATG 1 cut(s) 155
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 1 cut(s) 55
BstMBI GATC 1 cut(s) 52
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstUI CGCG 1 cut(s) 399
BstXI CCANNNNNNTGG 1 cut(s) 74
BstZI CGGCCG 1 cut(s) 133
BsuRI GGCC 3 cut(s) 135, 190, 396
BtsCI GGATG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 192
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 1 cut(s) 68
CaiI CAGNNNCTG 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 189
CseI GACGC 2 cut(s) 210, 360
Csp6I GTAC 3 cut(s) 138, 205, 414
CviAII CATG 3 cut(s) 49, 56, 71
CviJI RGCY 6 cut(s) 14, 46, 135, 171, 190, 396
CviKI_1 RGCY 6 cut(s) 14, 46, 135, 171, 190, 396
CviQI GTAC 3 cut(s) 138, 205, 414
DdeI CTNAG 1 cut(s) 42
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 2 cut(s) 133, 394
EagI CGGCCG 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 48
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 3 cut(s) 52, 59, 74
FaiI YATR 5 cut(s) 50, 57, 72, 404, 418
FatI CATG 3 cut(s) 48, 55, 70
Fnu4HI GCNGC 1 cut(s) 397
FokI GGATG 1 cut(s) 142
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 397
FspBI CTAG 1 cut(s) 347
GluI GCNGC 1 cut(s) 397
HaeIII GGCC 3 cut(s) 135, 190, 396
HgaI GACGC 2 cut(s) 210, 360
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 3 cut(s) 52, 59, 74
HinfI GANTC 3 cut(s) 176, 230, 350
HphI GGTGA 2 cut(s) 92, 110
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 245
Hpy99I CGWCG 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 196
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 1 cut(s) 42
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 3 cut(s) 52, 59, 74
Kzo9I GATC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 148
MaeI CTAG 1 cut(s) 347
MaeIII GTNAC 1 cut(s) 121
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 2 cut(s) 30, 275
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 2 cut(s) 248, 381
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 4 cut(s) 4, 76, 89, 163
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 75
MspCI CTTAAG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 149
NdeII GATC 1 cut(s) 52
NlaIII CATG 3 cut(s) 52, 59, 74
NlaIV GGNNCC 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PfeI GAWTC 2 cut(s) 176, 350
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 1 cut(s) 398
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 1 cut(s) 189
PstNI CAGNNNCTG 1 cut(s) 245
RsaI GTAC 3 cut(s) 139, 206, 415
RsaNI GTAC 3 cut(s) 138, 205, 414
RseI CAYNNNNRTG 1 cut(s) 75
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 1 cut(s) 397
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 1 cut(s) 189
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 48
SetI ASST 7 cut(s) 48, 87, 100, 120, 258, 276, 415
SmiMI CAYNNNNRTG 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 2 cut(s) 248, 381
SsiI CCGC 1 cut(s) 397
SspMI CTAG 1 cut(s) 347
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 9, 294
TasI AATT 2 cut(s) 248, 381
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 399
TfiI GAWTC 2 cut(s) 176, 350
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 1 cut(s) 121
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 2 cut(s) 17, 306
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 392
Vha464I CTTAAG 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.