FvH4_1g04461

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003933
Physical Location & Seq
Forward (+)
2375262 .. 2376003
742 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04461.t1

Sequence Viewer

Length: 660 bp
ATGAGCCAGGAACCAGAAACTGAAATCCTCAAAGAGGAACCACAACCTGCTGGACACTCTTTGATTCATATATCTGACAAGGATTCACATGTTGCTGGAGACGATTCTACTCAAGTCATCCAAGAAAAATCACAGGTTCCTGGAGAAGATTCTACTCAGATCATCCAAGAAAAACCACAGGTTCCTGGAGACTGTGCCACTCCAATCATCCAAGAAATACCAGAGGACGTTGTTGGGGACTTTCCCTCTGAAAATGACACAAAGATGCTTGTTTATTGCTTTAGATGCAAGAAGGATACCGATCATATTCCTGCGAGGTGCCCCAGCCGTCCAAAATATCCTAATTATTGCATGGTGTGCAATAAGAAGAGTGATCATGTAACTTCTGATTGCCCCTACGCAACTGAAGATCTCCTGAAGAAGCTTGCTTATTGCATGTTATGCGATAAGAACACTGATCATGACACTTTCAGTTGCCCTGACAAAGAAACTGGATTTGTATGGTACAATGGAGCTTATCTACCCAACGTTTGTGATCTTTGTAATAAGCTAGGTCACTTGGGTGAAGAATGCCGTCTTTTTGACAATGATCTTGAGTGTGATCTTCCGAGTGATGAGGATGACTGCCAGTTTGATGTAATATATCAGATTTCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

24.74

Weight (kDa)

4.4

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 55
AccB1I GGYRCC 1 cut(s) 318
AclI AACGTT 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 426, 437
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 88
AjnI CCWGG 3 cut(s) 6, 139, 184
AleI CACNNNNGTG 1 cut(s) 561
AluBI AGCT 3 cut(s) 424, 515, 550
AluI AGCT 3 cut(s) 424, 515, 550
Alw26I GTCTC 2 cut(s) 93, 183
AlwNI CAGNNNCTG 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 614, 646
AsuHPI GGTGA 1 cut(s) 575
BaeGI GKGCMC 1 cut(s) 323
BanI GGYRCC 1 cut(s) 318
BceAI ACGGC 2 cut(s) 312, 558
BciT130I CCWGG 3 cut(s) 8, 141, 186
BciVI GTATCC 1 cut(s) 289
BclI TGATCA 2 cut(s) 373, 457
BcoDI GTCTC 2 cut(s) 93, 183
BfaI CTAG 1 cut(s) 551
BfuAI ACCTGC 1 cut(s) 55
BfuI GTATCC 1 cut(s) 289
BglII AGATCT 1 cut(s) 409
Bme1390I CCNGG 3 cut(s) 8, 141, 186
BmiI GGNNCC 5 cut(s) 12, 39, 138, 183, 320
BmrFI CCNGG 3 cut(s) 8, 141, 186
BmsI GCATC 2 cut(s) 255, 275
BpmI CTGGAG 3 cut(s) 117, 162, 207
BpuEI CTTGAG 2 cut(s) 96, 614
BsaBI GATNNNNATC 1 cut(s) 300
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 496, 628
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 3 cut(s) 8, 141, 186
BseGI GGATG 4 cut(s) 117, 162, 207, 625
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 2 cut(s) 496, 628
BseSI GKGCMC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 323
BshNI GGYRCC 1 cut(s) 318
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 93, 183
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 575
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
BspCNI CTCAG 1 cut(s) 169
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 5 cut(s) 12, 39, 138, 183, 320
BspMI ACCTGC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 318
BsrI ACTGG 2 cut(s) 496, 628
BssMI GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
Bst2UI CCWGG 3 cut(s) 8, 141, 186
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 362
BstAPI GCANNNNNTGC 2 cut(s) 357, 441
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 117, 162, 207, 625
BstKTI GATC 8 cut(s) 162, 304, 376, 412, 460, 538, 592, 604
BstMAI GTCTC 2 cut(s) 93, 183
BstMBI GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
BstMWI GCNNNNNNNGC 3 cut(s) 285, 357, 441
BstNI CCWGG 3 cut(s) 8, 141, 186
BstNSI RCATGY 2 cut(s) 92, 439
BstSCI CCNGG 3 cut(s) 6, 139, 184
BstSLI GKGCMC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BsuI GTATCC 1 cut(s) 289
BtsCI GGATG 4 cut(s) 117, 162, 207, 625
BtsIMutI CAGTG 1 cut(s) 453
BveI ACCTGC 1 cut(s) 55
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 20
CciI TCATGA 1 cut(s) 460
Csp6I GTAC 1 cut(s) 505
CviAII CATG 5 cut(s) 89, 352, 377, 436, 461
CviJI RGCY 5 cut(s) 6, 327, 424, 515, 550
CviKI_1 RGCY 5 cut(s) 6, 327, 424, 515, 550
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 1 cut(s) 156
DpnI GATC 8 cut(s) 161, 303, 375, 411, 459, 537, 591, 603
DpnII GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 426, 437
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoRII CCWGG 3 cut(s) 6, 139, 184
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 5 cut(s) 92, 355, 380, 439, 464
FaqI GGGAC 1 cut(s) 251
FatI CATG 5 cut(s) 88, 351, 376, 435, 460
FbaI TGATCA 2 cut(s) 373, 457
FokI GGATG 4 cut(s) 104, 149, 194, 632
FspBI CTAG 1 cut(s) 551
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 3 cut(s) 117, 162, 207
Hin1II CATG 5 cut(s) 92, 355, 380, 439, 464
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 4 cut(s) 64, 83, 104, 149
HphI GGTGA 1 cut(s) 575
Hpy188I TCNGA 7 cut(s) 76, 159, 250, 388, 609, 648, 655
Hpy188III TCNNGA 3 cut(s) 415, 461, 593
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 2 cut(s) 228, 528
HpyCH4V TGCA 4 cut(s) 288, 351, 360, 435
HpyF10VI GCNNNNNNNGC 3 cut(s) 285, 357, 441
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 2 cut(s) 228, 528
Hsp92II CATG 5 cut(s) 92, 355, 380, 439, 464
Ksp22I TGATCA 2 cut(s) 373, 457
Kzo9I GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
LmnI GCTCC 1 cut(s) 512
LweI GCATC 2 cut(s) 255, 275
MaeI CTAG 1 cut(s) 551
MaeII ACGT 2 cut(s) 228, 528
MaeIII GTNAC 2 cut(s) 379, 554
MalI GATC 8 cut(s) 161, 303, 375, 411, 459, 537, 591, 603
MboI GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
MboII GAAGA 6 cut(s) 158, 379, 419, 430, 578, 596
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 1 cut(s) 343
MnlI CCTC 6 cut(s) 28, 38, 217, 256, 309, 610
MslI CAYNNNNRTG 2 cut(s) 263, 561
MspR9I CCNGG 3 cut(s) 8, 141, 186
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 3 cut(s) 8, 141, 186
MwoI GCNNNNNNNGC 3 cut(s) 285, 357, 441
NdeII GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
NlaIII CATG 5 cut(s) 92, 355, 380, 439, 464
NlaIV GGNNCC 5 cut(s) 12, 39, 138, 183, 320
NmuCI GTSAC 1 cut(s) 554
NspI RCATGY 2 cut(s) 92, 439
OliI CACNNNNGTG 1 cut(s) 561
PagI TCATGA 1 cut(s) 460
PciI ACATGT 1 cut(s) 88
PctI GAATGC 1 cut(s) 575
PfeI GAWTC 4 cut(s) 64, 83, 104, 149
PfoI TCCNGGA 2 cut(s) 139, 184
PscI ACATGT 1 cut(s) 88
Psp1406I AACGTT 1 cut(s) 528
Psp6I CCWGG 3 cut(s) 6, 139, 184
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 3 cut(s) 6, 139, 184
PspN4I GGNNCC 5 cut(s) 12, 39, 138, 183, 320
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 409
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
RseI CAYNNNNRTG 2 cut(s) 263, 561
Sau3AI GATC 8 cut(s) 159, 301, 373, 409, 457, 535, 589, 601
ScrFI CCNGG 3 cut(s) 8, 141, 186
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 2 cut(s) 255, 275
SmiMI CAYNNNNRTG 2 cut(s) 263, 561
SmlI CTYRAG 2 cut(s) 111, 593
SmoI CTYRAG 2 cut(s) 111, 593
Sse9I AATT 1 cut(s) 343
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 3 cut(s) 6, 139, 184
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 2 cut(s) 231, 531
TasI AATT 1 cut(s) 343
TfiI GAWTC 4 cut(s) 64, 83, 104, 149
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 1 cut(s) 56
TspRI CASTG 1 cut(s) 460
XagI CCTNNNNNAGG 1 cut(s) 32
XceI RCATGY 2 cut(s) 92, 439
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.