Rorug02G0007900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003933
Physical Location & Seq
Reverse (-)
666271 .. 668369
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0007900.1

Sequence Viewer

Length: 1116 bp
ATGGCCAAGGCAGTCACTTACAGCGTGACCACCTTAGGAGCCAAACCAGATGGCAAGACCGACTCAACTAAGGCCTTCCTTTCTGCATGGGCTAAAGCTTGTGCCTCGGTGAAACCCGCCGTGATTTACGTGCCGGCAGGGAGGTTCTTGCTCCGCAATGCCTTGTTCAGCGGACCATGCAAGAACAGCCGCATCACCTTCCGCATCGCCGGCACCCTCGTTGCTCCGTCTGATTACCGGGTCATCGGAAATGCCGGTAACTGGCTCCTCTTTCTGCACGTCAACGGAGTCACCATTTCCGGAGGAATTCTTGACGGTCAAGGCACTGGTTTGTGGGCTTGCAAGAACTCCGGCAAGAGTTGCCCCACGGGAGCCACGACATTGAGTTTTTCCAACTCCAACAATATTGTGGTTAGCGGATTGGTATCTCTAAACAGCCAAATGTTTCACATAGTCGTTAACGGTTGCAACAATGTGAAAATGCAAGGGGTTAAAGTATCGGCCTCCGGCAACAGCCCTAACACCGACGGTATCCATGTTCAAATGTCATCGGGTGTTACCATTCTCAACTCAAAGATCTCTACTGGTGATGATTGTGTATCAATTGGTCCCGGCACTTCTAACTTGTGGATTGAAAATGTTGCATGCGGACCAGGTCATGGAATTAGCATTGGGAGTCTAGGCAAAGACCTACAGGAAGCTGGTGTGCAAAATGTAACGGTGAAAACAGTGACATTTACCAATACTCAAAATGGTGTGAGAATCAAGTCCTGGGGGAGGGCTAGCACTGGATTTGCCAGGAACATTCTTTTCCAACATGCTGTCATGATTAACGTTCAAAATCCGATCGTCATCGATCAACAATATTGTCCCGACAAAAAAGGCTGCCCAGGCCAGGTTTCTGGAGTTAAAATCAGTGATGTGACATATCAAGACATTCATGGCACATCGGCAACAGAAGTTGCAGTGAAATTCGATTGTAGTTCAAAGTACCCATGTAGCAGAATCAGATTGGAGAATGTGAAGCTCACATACAAGAACCAAGGAGCTCTAGCTTCTTGTAGCAATGCTGGTGGAACATCTGCGGGTATGGTGCTGCCTACAAGTTGTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

371

Amino Acids

38.73

Weight (kDa)

9.33

Isoelectric Point (pI)

26.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 8 - 259 2.4e-14 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 34 - 357 9.4e-89 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AccB7I CCANNNNNTGG 1 cut(s) 659
AccIII TCCGGA 1 cut(s) 299
AciI CCGC 8 cut(s) 117, 154, 171, 190, 202, 417, 648, 1085
AclI AACGTT 1 cut(s) 834
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 306, 971
AfaI GTAC 1 cut(s) 992
AfiI CCNNNNNNNGG 4 cut(s) 261, 659, 777, 895
AgsI TTSAA 4 cut(s) 542, 635, 839, 987
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 5 cut(s) 652, 770, 797, 889, 894
AluBI AGCT 5 cut(s) 98, 701, 1027, 1049, 1055
AluI AGCT 5 cut(s) 98, 701, 1027, 1049, 1055
Alw21I GWGCWC 1 cut(s) 1051
Aor13HI TCCGGA 1 cut(s) 299
AoxI GGCC 4 cut(s) 3, 72, 501, 892
ApeKI GCWGC 2 cut(s) 885, 1096
ApoI RAATTY 2 cut(s) 306, 971
AspS9I GGNCC 3 cut(s) 173, 608, 650
AsuC2I CCSGG 2 cut(s) 239, 612
AsuHPI GGTGA 5 cut(s) 121, 187, 283, 599, 733
AsuNHI GCTAGC 1 cut(s) 782
AvaII GGWCC 3 cut(s) 173, 608, 650
AxyI CCTNAGG 1 cut(s) 34
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 1051
BarI GAAGNNNNNNTAC 2 cut(s) 1016, 1048
Bbv12I GWGCWC 1 cut(s) 1051
BbvI GCAGC 2 cut(s) 872, 1083
BccI CCATC 1 cut(s) 44
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 5 cut(s) 654, 772, 799, 891, 896
BciVI GTATCC 1 cut(s) 542
BcnI CCSGG 2 cut(s) 239, 612
BfaI CTAG 3 cut(s) 680, 783, 1052
BfmI CTRYAG 2 cut(s) 692, 1112
BfuI GTATCC 1 cut(s) 542
BglII AGATCT 1 cut(s) 576
BisI GCNGC 3 cut(s) 190, 886, 1097
BlsI GCNGC 3 cut(s) 191, 887, 1098
Bme1390I CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
Bme18I GGWCC 3 cut(s) 173, 608, 650
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 3 cut(s) 173, 608, 650
BmiI GGNNCC 5 cut(s) 40, 214, 266, 373, 610
BmrFI CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
BmsI GCATC 2 cut(s) 201, 213
BmtI GCTAGC 1 cut(s) 786
BpmI CTGGAG 1 cut(s) 924
BpuMI CCSGG 2 cut(s) 239, 612
Bsa29I ATCGAT 1 cut(s) 855
BsaAI YACGTR 1 cut(s) 130
BsaBI GATNNNNATC 2 cut(s) 424, 851
BsaJI CCNNGG 6 cut(s) 6, 105, 366, 771, 889, 1042
BsaWI WCCGGW 1 cut(s) 299
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 659, 777, 895
Bse118I RCCGGY 3 cut(s) 133, 209, 254
Bse1I ACTGG 4 cut(s) 266, 331, 589, 793
Bse21I CCTNAGG 1 cut(s) 34
Bse3DI GCAATG 2 cut(s) 163, 1072
Bse8I GATNNNNATC 2 cut(s) 424, 851
BseAI TCCGGA 1 cut(s) 299
BseBI CCWGG 5 cut(s) 654, 772, 799, 891, 896
BseCI ATCGAT 1 cut(s) 855
BseDI CCNNGG 6 cut(s) 6, 105, 366, 771, 889, 1042
BseJI GATNNNNATC 2 cut(s) 424, 851
BseLI CCNNNNNNNGG 4 cut(s) 261, 659, 777, 895
BseMI GCAATG 2 cut(s) 163, 1072
BseNI ACTGG 4 cut(s) 266, 331, 589, 793
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 872, 1083
BsgI GTGCAG 1 cut(s) 260
Bsh1285I CGRYCG 1 cut(s) 849
BshFI GGCC 4 cut(s) 5, 74, 503, 894
BshNI GGYRCC 1 cut(s) 212
BshVI ATCGAT 1 cut(s) 855
BsiEI CGRYCG 1 cut(s) 849
BsiHKAI GWGCWC 1 cut(s) 1051
BsiSI CCGG 8 cut(s) 134, 210, 238, 255, 300, 351, 507, 612
BslFI GGGAC 2 cut(s) 594, 855
BslI CCNNNNNNNGG 4 cut(s) 261, 659, 777, 895
BsmFI GGGAC 2 cut(s) 594, 855
BsnI GGCC 4 cut(s) 5, 74, 503, 894
Bsp1286I GDGCHC 1 cut(s) 1051
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 3 cut(s) 576, 846, 856
BspACI CCGC 8 cut(s) 117, 154, 171, 190, 202, 417, 648, 1085
BspANI GGCC 4 cut(s) 5, 74, 503, 894
BspDI ATCGAT 1 cut(s) 855
BspEI TCCGGA 1 cut(s) 299
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 5 cut(s) 40, 214, 266, 373, 610
BspOI GCTAGC 1 cut(s) 786
BspT107I GGYRCC 1 cut(s) 212
BsrDI GCAATG 2 cut(s) 163, 1072
BsrFI RCCGGY 3 cut(s) 133, 209, 254
BsrI ACTGG 4 cut(s) 266, 331, 589, 793
BssAI RCCGGY 3 cut(s) 133, 209, 254
BssECI CCNNGG 6 cut(s) 6, 105, 366, 771, 889, 1042
BssMI GATC 3 cut(s) 576, 846, 856
BssT1I CCWWGG 2 cut(s) 6, 1042
Bst2UI CCWGG 5 cut(s) 654, 772, 799, 891, 896
Bst4CI ACNGT 5 cut(s) 317, 464, 530, 721, 730
BstAPI GCANNNNNTGC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 5 cut(s) 135, 211, 340, 646, 784
BstDEI CTNAG 2 cut(s) 34, 69
BstDSI CCRYGG 1 cut(s) 366
BstENI CCTNNNNNAGG 1 cut(s) 775
BstKTI GATC 3 cut(s) 579, 849, 859
BstMBI GATC 3 cut(s) 576, 846, 856
BstMCI CGRYCG 1 cut(s) 849
BstMWI GCNNNNNNNGC 5 cut(s) 177, 186, 210, 360, 891
BstNI CCWGG 5 cut(s) 654, 772, 799, 891, 896
BstNSI RCATGY 2 cut(s) 648, 821
BstSCI CCNGG 7 cut(s) 237, 610, 652, 770, 797, 889, 894
BstSFI CTRYAG 2 cut(s) 692, 1112
BstV1I GCAGC 2 cut(s) 872, 1083
BstX2I RGATCY 1 cut(s) 576
BstXI CCANNNNNNTGG 1 cut(s) 902
BstYI RGATCY 1 cut(s) 576
Bsu15I ATCGAT 1 cut(s) 855
Bsu36I CCTNAGG 1 cut(s) 34
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 4 cut(s) 5, 74, 503, 894
BsuTUI ATCGAT 1 cut(s) 855
BtgI CCRYGG 1 cut(s) 366
BtgZI GCGATG 1 cut(s) 190
BtrI CACGTC 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 5 cut(s) 324, 735, 786, 922, 972
Cac8I GCNNGC 5 cut(s) 135, 211, 340, 646, 784
CciI TCATGA 1 cut(s) 825
Cfr10I RCCGGY 3 cut(s) 133, 209, 254
Cfr13I GGNCC 3 cut(s) 173, 608, 650
ClaI ATCGAT 1 cut(s) 855
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 1 cut(s) 991
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviAII CATG 9 cut(s) 87, 177, 536, 645, 659, 818, 826, 941, 996
CviQI GTAC 1 cut(s) 991
DdeI CTNAG 2 cut(s) 34, 69
DpnI GATC 3 cut(s) 578, 848, 858
DpnII GATC 3 cut(s) 576, 846, 856
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 1049
Eco130I CCWWGG 2 cut(s) 6, 1042
Eco147I AGGCCT 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 1051
Eco47I GGWCC 3 cut(s) 173, 608, 650
Eco53kI GAGCTC 1 cut(s) 1049
Eco81I CCTNAGG 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 1049
EcoNI CCTNNNNNAGG 1 cut(s) 775
EcoRI GAATTC 1 cut(s) 306
EcoRII CCWGG 5 cut(s) 652, 770, 797, 889, 894
EcoT14I CCWWGG 2 cut(s) 6, 1042
EcoT38I GRGCYC 1 cut(s) 1051
ErhI CCWWGG 2 cut(s) 6, 1042
FaeI CATG 9 cut(s) 90, 180, 539, 648, 662, 821, 829, 944, 999
FaqI GGGAC 2 cut(s) 594, 855
FatI CATG 9 cut(s) 86, 176, 535, 644, 658, 817, 825, 940, 995
FauI CCCGC 2 cut(s) 124, 1078
Fnu4HI GCNGC 3 cut(s) 190, 886, 1097
FriOI GRGCYC 1 cut(s) 1051
Fsp4HI GCNGC 3 cut(s) 190, 886, 1097
FspBI CTAG 3 cut(s) 680, 783, 1052
GluI GCNGC 3 cut(s) 190, 886, 1097
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 4 cut(s) 5, 74, 503, 894
HapII CCGG 8 cut(s) 134, 210, 238, 255, 300, 351, 507, 612
Hin1II CATG 9 cut(s) 90, 180, 539, 648, 662, 821, 829, 944, 999
HincII GTYRAC 2 cut(s) 283, 460
HindII GTYRAC 2 cut(s) 283, 460
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 5 cut(s) 62, 288, 676, 762, 1005
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 8 cut(s) 134, 210, 238, 255, 300, 351, 507, 612
HphI GGTGA 5 cut(s) 121, 187, 283, 599, 733
Hpy166II GTNNAC 2 cut(s) 283, 460
Hpy188I TCNGA 4 cut(s) 232, 248, 846, 1010
Hpy188III TCNNGA 6 cut(s) 300, 311, 826, 872, 903, 932
Hpy8I GTNNAC 2 cut(s) 283, 460
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 2 cut(s) 85, 208
HpyCH4III ACNGT 5 cut(s) 317, 464, 530, 721, 730
HpyCH4IV ACGT 3 cut(s) 129, 279, 834
HpyCH4V TGCA 9 cut(s) 86, 180, 277, 342, 468, 484, 644, 709, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 186, 210, 360, 891
HpyF3I CTNAG 2 cut(s) 34, 69
HpySE526I ACGT 3 cut(s) 129, 279, 834
Hsp92II CATG 9 cut(s) 90, 180, 539, 648, 662, 821, 829, 944, 999
Kpn2I TCCGGA 1 cut(s) 299
KroI GCCGGC 2 cut(s) 133, 209
KroNI GCCGGC 2 cut(s) 135, 211
KspAI GTTAAC 1 cut(s) 460
Kzo9I GATC 3 cut(s) 576, 846, 856
LmnI GCTCC 6 cut(s) 38, 156, 229, 270, 371, 1046
Lsp1109I GCAGC 2 cut(s) 872, 1083
LweI GCATC 2 cut(s) 201, 213
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 3 cut(s) 680, 783, 1052
MaeII ACGT 3 cut(s) 129, 279, 834
MaeIII GTNAC 8 cut(s) 13, 25, 257, 289, 556, 715, 730, 922
MalI GATC 3 cut(s) 578, 848, 858
MboI GATC 3 cut(s) 576, 846, 856
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 1 cut(s) 576
MhlI GDGCHC 1 cut(s) 1051
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 306, 603, 663, 971
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 56, 297, 685
MmeI TCCRAC 3 cut(s) 417, 423, 838
MnlI CCTC 7 cut(s) 115, 135, 227, 278, 296, 514, 771
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 299
MroNI GCCGGC 2 cut(s) 133, 209
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 459, 492, 831, 909
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 171
MspI CCGG 8 cut(s) 134, 210, 238, 255, 300, 351, 507, 612
MspR9I CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
MunI CAATTG 1 cut(s) 603
MvaI CCWGG 5 cut(s) 654, 772, 799, 891, 896
MwoI GCNNNNNNNGC 5 cut(s) 177, 186, 210, 360, 891
NaeI GCCGGC 2 cut(s) 135, 211
NciI CCSGG 2 cut(s) 239, 612
NdeII GATC 3 cut(s) 576, 846, 856
NgoMIV GCCGGC 2 cut(s) 133, 209
NheI GCTAGC 1 cut(s) 782
NlaIII CATG 9 cut(s) 90, 180, 539, 648, 662, 821, 829, 944, 999
NlaIV GGNNCC 5 cut(s) 40, 214, 266, 373, 610
NmuCI GTSAC 5 cut(s) 13, 25, 289, 730, 922
NspI RCATGY 2 cut(s) 648, 821
PaeI GCATGC 1 cut(s) 648
PagI TCATGA 1 cut(s) 825
PceI AGGCCT 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PdiI GCCGGC 2 cut(s) 135, 211
PfeI GAWTC 2 cut(s) 762, 1005
PflFI GACNNNGTC 1 cut(s) 654
PflMI CCANNNNNTGG 1 cut(s) 659
PkrI GCNGC 3 cut(s) 191, 887, 1098
Ple19I CGATCG 1 cut(s) 849
PleI GAGTC 3 cut(s) 56, 296, 684
PpsI GAGTC 3 cut(s) 56, 296, 684
Ppu21I YACGTR 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 1051
Psp1406I AACGTT 1 cut(s) 834
Psp6I CCWGG 5 cut(s) 652, 770, 797, 889, 894
PspGI CCWGG 5 cut(s) 652, 770, 797, 889, 894
PspN4I GGNNCC 5 cut(s) 40, 214, 266, 373, 610
PspPI GGNCC 3 cut(s) 173, 608, 650
PsuI RGATCY 1 cut(s) 576
PsyI GACNNNGTC 1 cut(s) 654
PvuI CGATCG 1 cut(s) 849
RsaI GTAC 1 cut(s) 992
RsaNI GTAC 1 cut(s) 991
SacI GAGCTC 1 cut(s) 1051
SaqAI TTAA 4 cut(s) 459, 492, 831, 909
SatI GCNGC 3 cut(s) 190, 886, 1097
Sau3AI GATC 3 cut(s) 576, 846, 856
Sau96I GGNCC 3 cut(s) 173, 608, 650
SchI GAGTC 3 cut(s) 56, 297, 685
ScrFI CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
SduI GDGCHC 1 cut(s) 1051
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 2 cut(s) 201, 213
SfcI CTRYAG 2 cut(s) 692, 1112
SinI GGWCC 3 cut(s) 173, 608, 650
SphI GCATGC 1 cut(s) 648
Sse9I AATT 4 cut(s) 306, 603, 663, 971
SseBI AGGCCT 1 cut(s) 74
SsiI CCGC 8 cut(s) 117, 154, 171, 190, 202, 417, 648, 1085
SspI AATATT 2 cut(s) 406, 866
SspMI CTAG 3 cut(s) 680, 783, 1052
SstI GAGCTC 1 cut(s) 1051
StuI AGGCCT 1 cut(s) 74
StyD4I CCNGG 7 cut(s) 237, 610, 652, 770, 797, 889, 894
StyI CCWWGG 2 cut(s) 6, 1042
TaaI ACNGT 5 cut(s) 317, 464, 530, 721, 730
TaiI ACGT 3 cut(s) 132, 282, 837
TaqI TCGA 2 cut(s) 855, 975
TaqII GACCGA 1 cut(s) 74
TasI AATT 4 cut(s) 306, 603, 663, 971
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 2 cut(s) 762, 1005
Tru1I TTAA 4 cut(s) 459, 492, 831, 909
Tru9I TTAA 4 cut(s) 459, 492, 831, 909
TscAI CASTG 5 cut(s) 331, 735, 793, 922, 972
TseFI GTSAC 5 cut(s) 13, 25, 289, 730, 922
TseI GCWGC 2 cut(s) 885, 1096
Tsp45I GTSAC 5 cut(s) 13, 25, 289, 730, 922
TspDTI ATGAA 1 cut(s) 929
TspGWI ACGGA 2 cut(s) 216, 300
TspRI CASTG 5 cut(s) 331, 735, 793, 922, 972
Tth111I GACNNNGTC 1 cut(s) 654
Van91I CCANNNNNTGG 1 cut(s) 659
VpaK11BI GGWCC 3 cut(s) 173, 608, 650
XagI CCTNNNNNAGG 1 cut(s) 775
XapI RAATTY 2 cut(s) 306, 971
XceI RCATGY 2 cut(s) 648, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 3 cut(s) 680, 783, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.