FvH4_1g19820

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003589
Physical Location & Seq
Reverse (-)
12008606 .. 12010578
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19820.t1

Sequence Viewer

Length: 789 bp
ATGATAAAACCAAATCAAATGCATGCATCAGCAATCACATCCACACCAATACCTAATTCCCATTTCCGATTCCAATACAACAGGGCAGCTCCGATTAGGGTTCGTCTCTGGGCCTCGATTCTCAGCATTGCCGCCGTGGCCAACAGAGCCATCATGGTCACCACCACCGCCGCCAGCAGCAGCAACAGTCGCGGTGGCGCGTTCACCACCCTCAAAGAGACCGTCACCTTCGAAAAGGAGATCAAGAAGAGCAAATTCATCGCCCTCGCCGCTTCCGTCTCCGACGAACAATCCGCCTTCTCCTTCCTCTCTCAGGTTAGGGATGCTCGTGCTACTCACAATTGCTGGGCATACAAGGTGGGAGATCAGTACCGGTCTAATGATGATGGTGAGCCGTCTGGGACGGCTGGGAAGCCCATTCAGTCAGCTATTGAGTCTTCAGGAATTGATAGAGTAATGGTGGTGGTCATTCGGTATTTTGGAGGTATTAAGTTGGGGACTGGAGGACTAGTCAGGGCATATGGAGGAGTAACGTCGGAATGCTTGAGAAATGCCCCGACTCATCTTGTGAAATCGAAGGTAAGAATGGGGGTAGAGGTTCCATTTGATCTTATCGGCATTCTATACCATCAGCTACAATCTTTTCAAGCTGTAGACATCAAACAAGATTACGAGATTGGTAAAGACGGTATAACTATGGTTACCTTTCAAGTTGATTTTGACCGTGTTGAGAAATTAGAGGATGCAATCAAAACCAACTGTAGCCGGGAACTAGTGTTCTATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

263

Amino Acids

28.84

Weight (kDa)

9.3

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 82 - 182 2.1e-34 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 654
AccII CGCG 2 cut(s) 192, 200
AciI CCGC 6 cut(s) 132, 168, 171, 192, 270, 294
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 1 cut(s) 313
AgeI ACCGGT 1 cut(s) 372
AgsI TTSAA 2 cut(s) 647, 710
AhlI ACTAGT 2 cut(s) 508, 772
AjuI GAANNNNNNNTTGG 2 cut(s) 40, 72
AloI GAACNNNNNNTCC 1 cut(s) 761
AluBI AGCT 4 cut(s) 89, 428, 634, 650
AluI AGCT 4 cut(s) 89, 428, 634, 650
Alw26I GTCTC 3 cut(s) 110, 212, 283
AoxI GGCC 2 cut(s) 111, 138
ApeKI GCWGC 3 cut(s) 86, 177, 180
ApoI RAATTY 1 cut(s) 254
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AsiGI ACCGGT 1 cut(s) 372
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 111
AsuC2I CCSGG 1 cut(s) 767
AsuHPI GGTGA 4 cut(s) 151, 196, 217, 401
AsuII TTCGAA 1 cut(s) 231
BalI TGGCCA 1 cut(s) 140
BauI CACGAG 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 98, 189, 192
BccI CCATC 3 cut(s) 158, 380, 636
BceAI ACGGC 3 cut(s) 119, 379, 420
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BcnI CCSGG 1 cut(s) 767
BcoDI GTCTC 3 cut(s) 110, 212, 283
BcuI ACTAGT 2 cut(s) 508, 772
BfaI CTAG 2 cut(s) 509, 773
BfmI CTRYAG 2 cut(s) 651, 760
BglI GCCNNNNNGGC 1 cut(s) 137
BisI GCNGC 6 cut(s) 87, 132, 171, 178, 181, 270
BlsI GCNGC 6 cut(s) 88, 133, 172, 179, 182, 271
Bme1390I CCNGG 1 cut(s) 767
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 1 cut(s) 600
BmrFI CCNGG 1 cut(s) 767
BmsI GCATC 3 cut(s) 35, 313, 733
BpiI GAAGAC 1 cut(s) 429
BpmI CTGGAG 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 565
BpuMI CCSGG 1 cut(s) 767
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 3 cut(s) 38, 328, 748
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 136, 326
BseNI ACTGG 1 cut(s) 505
BseRI GAGGAG 1 cut(s) 540
BseXI GCAGC 3 cut(s) 98, 189, 192
BseYI CCCAGC 2 cut(s) 345, 407
Bsh1236I CGCG 2 cut(s) 192, 200
BshFI GGCC 2 cut(s) 113, 140
BshTI ACCGGT 1 cut(s) 372
BsiSI CCGG 2 cut(s) 373, 766
BslFI GGGAC 2 cut(s) 415, 511
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 3 cut(s) 110, 212, 283
BsmBI CGTCTC 2 cut(s) 110, 283
BsmFI GGGAC 2 cut(s) 415, 511
BsmI GAATGC 2 cut(s) 545, 618
BsnI GGCC 2 cut(s) 113, 140
Bso31I GGTCTC 1 cut(s) 212
Bsp119I TTCGAA 1 cut(s) 231
Bsp143I GATC 3 cut(s) 240, 364, 607
BspACI CCGC 6 cut(s) 132, 168, 171, 192, 270, 294
BspANI GGCC 2 cut(s) 113, 140
BspCNI CTCAG 2 cut(s) 135, 325
BspFNI CGCG 2 cut(s) 192, 200
BspLI GGNNCC 1 cut(s) 600
BspQI GCTCTTC 1 cut(s) 242
BspT104I TTCGAA 1 cut(s) 231
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 126
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 1 cut(s) 505
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 3 cut(s) 240, 364, 607
BssSI CACGAG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 327
Bst4CI ACNGT 5 cut(s) 188, 223, 689, 725, 761
Bst6I CTCTTC 1 cut(s) 242
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 24, 175
BstDEI CTNAG 2 cut(s) 122, 312
BstDSI CCRYGG 1 cut(s) 135
BstEII GGTNACC 2 cut(s) 157, 700
BstENI CCTNNNNNAGG 1 cut(s) 311
BstF5I GGATG 3 cut(s) 38, 328, 748
BstFNI CGCG 2 cut(s) 192, 200
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 3 cut(s) 243, 367, 610
BstMAI GTCTC 3 cut(s) 110, 212, 283
BstMBI GATC 3 cut(s) 240, 364, 607
BstMWI GCNNNNNNNGC 4 cut(s) 137, 146, 189, 269
BstNSI RCATGY 1 cut(s) 26
BstPI GGTNACC 2 cut(s) 157, 700
BstSCI CCNGG 1 cut(s) 765
BstSFI CTRYAG 2 cut(s) 651, 760
BstUI CGCG 2 cut(s) 192, 200
BstV1I GCAGC 3 cut(s) 98, 189, 192
BstV2I GAAGAC 1 cut(s) 429
BsuRI GGCC 2 cut(s) 113, 140
BtgI CCRYGG 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 38, 328, 748
Cac8I GCNNGC 2 cut(s) 24, 175
CfoI GCGC 1 cut(s) 200
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 111
Csp6I GTAC 1 cut(s) 370
CspAI ACCGGT 1 cut(s) 372
CviAII CATG 2 cut(s) 23, 154
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 2 cut(s) 122, 312
DpnI GATC 3 cut(s) 242, 366, 609
DpnII GATC 3 cut(s) 240, 364, 607
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
EciI GGCGGA 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 423
Eco91I GGTNACC 2 cut(s) 157, 700
EcoNI CCTNNNNNAGG 1 cut(s) 311
EcoO65I GGTNACC 2 cut(s) 157, 700
EcoT22I ATGCAT 2 cut(s) 24, 28
Esp3I CGTCTC 2 cut(s) 110, 283
FaeI CATG 2 cut(s) 26, 157
FaiI YATR 9 cut(s) 24, 155, 352, 520, 522, 625, 692, 698, 783
FaqI GGGAC 2 cut(s) 415, 511
FatI CATG 2 cut(s) 22, 153
FauNDI CATATG 1 cut(s) 520
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 6 cut(s) 87, 132, 171, 178, 181, 270
FokI GGATG 3 cut(s) 25, 335, 755
Fsp4HI GCNGC 6 cut(s) 87, 132, 171, 178, 181, 270
FspBI CTAG 2 cut(s) 509, 773
GlaI GCGC 1 cut(s) 199
GluI GCNGC 6 cut(s) 87, 132, 171, 178, 181, 270
GsaI CCCAGC 2 cut(s) 349, 411
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 2 cut(s) 113, 140
HapII CCGG 2 cut(s) 373, 766
HhaI GCGC 1 cut(s) 200
Hin1II CATG 2 cut(s) 26, 157
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 4 cut(s) 69, 118, 434, 559
HpaII CCGG 2 cut(s) 373, 766
HphI GGTGA 4 cut(s) 151, 196, 217, 401
Hpy166II GTNNAC 2 cut(s) 204, 655
Hpy188I TCNGA 4 cut(s) 68, 93, 283, 538
Hpy188III TCNNGA 2 cut(s) 244, 441
Hpy8I GTNNAC 2 cut(s) 204, 655
Hpy99I CGWCG 2 cut(s) 287, 538
HpyAV CCTTC 4 cut(s) 238, 307, 313, 571
HpyCH4III ACNGT 5 cut(s) 188, 223, 689, 725, 761
HpyCH4IV ACGT 1 cut(s) 533
HpyCH4V TGCA 3 cut(s) 22, 26, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 146, 189, 269
HpyF3I CTNAG 2 cut(s) 122, 312
HpySE526I ACGT 1 cut(s) 533
Hsp92II CATG 2 cut(s) 26, 157
HspAI GCGC 1 cut(s) 198
Kzo9I GATC 3 cut(s) 240, 364, 607
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 3 cut(s) 98, 189, 192
LweI GCATC 3 cut(s) 35, 313, 733
MaeI CTAG 2 cut(s) 509, 773
MaeII ACGT 1 cut(s) 533
MaeIII GTNAC 4 cut(s) 157, 223, 529, 700
MalI GATC 3 cut(s) 242, 366, 609
MboI GATC 3 cut(s) 240, 364, 607
MboII GAAGA 2 cut(s) 259, 429
MfeI CAATTG 1 cut(s) 340
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 5 cut(s) 55, 254, 340, 444, 734
MluNI TGGCCA 1 cut(s) 140
MlyI GAGTC 2 cut(s) 443, 553
MmeI TCCRAC 2 cut(s) 306, 516
MnlI CCTC 9 cut(s) 124, 221, 275, 317, 476, 497, 518, 589, 733
Mox20I TGGCCA 1 cut(s) 140
Mph1103I ATGCAT 2 cut(s) 24, 28
MscI TGGCCA 1 cut(s) 140
MseI TTAA 1 cut(s) 489
Msp20I TGGCCA 1 cut(s) 140
MspI CCGG 2 cut(s) 373, 766
MspR9I CCNGG 1 cut(s) 767
MunI CAATTG 1 cut(s) 340
Mva1269I GAATGC 2 cut(s) 545, 618
MvnI CGCG 2 cut(s) 192, 200
MwoI GCNNNNNNNGC 4 cut(s) 137, 146, 189, 269
NciI CCSGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 520
NdeII GATC 3 cut(s) 240, 364, 607
NlaIII CATG 2 cut(s) 26, 157
NlaIV GGNNCC 1 cut(s) 600
NmuCI GTSAC 2 cut(s) 157, 223
NsiI ATGCAT 2 cut(s) 24, 28
NspI RCATGY 1 cut(s) 26
NspV TTCGAA 1 cut(s) 231
PaeI GCATGC 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 2 cut(s) 545, 618
PfeI GAWTC 2 cut(s) 69, 118
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 6 cut(s) 88, 133, 172, 179, 182, 271
PleI GAGTC 2 cut(s) 442, 553
PpsI GAGTC 2 cut(s) 442, 553
PspEI GGTNACC 2 cut(s) 157, 700
PspFI CCCAGC 2 cut(s) 345, 407
PspN4I GGNNCC 1 cut(s) 600
PspPI GGNCC 1 cut(s) 111
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 6 cut(s) 87, 132, 171, 178, 181, 270
Sau3AI GATC 3 cut(s) 240, 364, 607
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 2 cut(s) 443, 553
ScrFI CCNGG 1 cut(s) 767
SfaNI GCATC 3 cut(s) 35, 313, 733
SfcI CTRYAG 2 cut(s) 651, 760
SfuI TTCGAA 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 544
SmoI CTYRAG 1 cut(s) 544
SpeI ACTAGT 2 cut(s) 508, 772
SphI GCATGC 1 cut(s) 26
Sse9I AATT 5 cut(s) 55, 254, 340, 444, 734
SsiI CCGC 6 cut(s) 132, 168, 171, 192, 270, 294
SspMI CTAG 2 cut(s) 509, 773
StyD4I CCNGG 1 cut(s) 765
TaaI ACNGT 5 cut(s) 188, 223, 689, 725, 761
TaiI ACGT 1 cut(s) 536
TaqI TCGA 3 cut(s) 116, 231, 575
TasI AATT 5 cut(s) 55, 254, 340, 444, 734
TauI GCSGC 3 cut(s) 134, 173, 272
TfiI GAWTC 2 cut(s) 69, 118
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 2 cut(s) 157, 223
TseI GCWGC 3 cut(s) 86, 177, 180
Tsp45I GTSAC 2 cut(s) 157, 223
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 311
XapI RAATTY 1 cut(s) 254
XceI RCATGY 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 2 cut(s) 509, 773
Zsp2I ATGCAT 2 cut(s) 24, 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.