Rw2G017500

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003589
Physical Location & Seq
Reverse (-)
21606878 .. 21608303
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017500.1

Sequence Viewer

Length: 789 bp
ATGAATTTGGCTAATCCAAAACAAGCCTCAAGGCTTAAGCACGTTGATACGATAAAATCAAATCAAATGCCGGCAGCGGCAGCATCAATCACACCAATACCTAATTTCCATTTCCATTCCCACAAGGTAGCTCCCATTAGGTTTCGTCTCTGGGCCTCGATTCTCAGCATTGCCGCCGTATCCAACCGAGCCACCATGGTCACCACTGCCGCCACCAGCAACAGCTGTCGCGGTGGCGCGTATTCCACCATCAAAGAGCGCGTCACCTTCGAAAAAGAGATCAAGAAGAGCAAATTCATCGCCCTCGCCGCTTCCGTTAGGGATCCTCGTGCTACTCACAATTGCTGGGCATACAAGGTGGGAGATCAGTACCGGTCTAATGATGACGGTGAGCCATCTGGAACGGCCGGGAAGCCCATTCATTCTGCTATTGAGTCTTCAGGAGTTGATAGAGTAATGGTGGTGGTCATCCGTTATTTTGGAGGAATTAAATTGGGGACTGGAGGACTAGTCAGGGCATATGGAGGAGTAACATCGGAATGCTTGAGAAATGCCCCAACTCATCTCGTAAAATCGAAGGTAAGAATGGGTGTAGAGGTTCCATTTGATCTTATCGGCATTCTATACCATCAGCTACAATCTTTTCAAGTTGAAGACATCAAACAAGATTATGAGACCGGTAAAGATGGTATCACCATGGTTACCTTTCAAGTTGATTTTGACTGTGTTGATAAATTAGAGGATGCTATCAAAAATAACTGTAGCCGGGAGTTAGTGTTCTATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

262

Amino Acids

28.86

Weight (kDa)

9.31

Isoelectric Point (pI)

28.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 99 - 182 1e-28 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 231, 239, 261
AciI CCGC 5 cut(s) 77, 174, 210, 231, 309
AclWI GGATC 2 cut(s) 317, 330
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 2 cut(s) 4, 293
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 1 cut(s) 371
AflII CTTAAG 1 cut(s) 35
AgeI ACCGGT 2 cut(s) 372, 677
AgsI TTSAA 3 cut(s) 647, 653, 710
AhlI ACTAGT 1 cut(s) 508
AloI GAACNNNNNNTCC 1 cut(s) 761
AluBI AGCT 3 cut(s) 131, 225, 634
AluI AGCT 3 cut(s) 131, 225, 634
Alw26I GTCTC 2 cut(s) 152, 668
AlwI GGATC 2 cut(s) 317, 330
AoxI GGCC 2 cut(s) 153, 405
ApeKI GCWGC 2 cut(s) 74, 80
ApoI RAATTY 2 cut(s) 4, 293
ArsI GACNNNNNNTTYG 2 cut(s) 246, 278
AsiGI ACCGGT 2 cut(s) 372, 677
AspLEI GCGC 2 cut(s) 239, 261
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 2 cut(s) 409, 767
AsuHPI GGTGA 4 cut(s) 193, 256, 401, 685
AsuII TTCGAA 1 cut(s) 270
BamHI GGATCC 1 cut(s) 322
BauI CACGAG 1 cut(s) 327
BbsI GAAGAC 2 cut(s) 429, 660
BbvI GCAGC 2 cut(s) 86, 92
BccI CCATC 4 cut(s) 257, 403, 636, 680
BceAI ACGGC 2 cut(s) 161, 420
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciVI GTATCC 1 cut(s) 190
BcnI CCSGG 2 cut(s) 409, 767
BcoDI GTCTC 2 cut(s) 152, 668
BcuI ACTAGT 1 cut(s) 508
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 760
BfrI CTTAAG 1 cut(s) 35
BfuI GTATCC 1 cut(s) 190
BisI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
BlsI GCNGC 6 cut(s) 76, 79, 82, 175, 211, 310
Bme1390I CCNGG 2 cut(s) 409, 767
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 2 cut(s) 324, 600
BmrFI CCNGG 2 cut(s) 409, 767
BmsI GCATC 2 cut(s) 92, 733
BpiI GAAGAC 2 cut(s) 429, 660
BpmI CTGGAG 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 13, 565
BpuMI CCSGG 2 cut(s) 409, 767
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 2 cut(s) 195, 696
BsaWI WCCGGW 2 cut(s) 372, 677
BsaXI ACNNNNNCTCC 3 cut(s) 495, 525, 761
Bse118I RCCGGY 3 cut(s) 70, 372, 677
Bse1I ACTGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 195, 696
BseGI GGATG 2 cut(s) 468, 748
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 1 cut(s) 505
BseRI GAGGAG 1 cut(s) 540
BseX3I CGGCCG 1 cut(s) 405
BseXI GCAGC 2 cut(s) 86, 92
BseYI CCCAGC 1 cut(s) 345
Bsh1236I CGCG 3 cut(s) 231, 239, 261
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 2 cut(s) 155, 407
BshTI ACCGGT 2 cut(s) 372, 677
BsiEI CGRYCG 1 cut(s) 408
BsiSI CCGG 5 cut(s) 71, 373, 408, 678, 766
BslFI GGGAC 1 cut(s) 511
BsmAI GTCTC 2 cut(s) 152, 668
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 511
BsmI GAATGC 2 cut(s) 545, 618
BsnI GGCC 2 cut(s) 155, 407
Bso31I GGTCTC 1 cut(s) 668
Bsp119I TTCGAA 1 cut(s) 270
Bsp143I GATC 4 cut(s) 279, 322, 364, 607
Bsp19I CCATGG 2 cut(s) 195, 696
BspACI CCGC 5 cut(s) 77, 174, 210, 231, 309
BspANI GGCC 2 cut(s) 155, 407
BspCNI CTCAG 1 cut(s) 177
BspFNI CGCG 3 cut(s) 231, 239, 261
BspLI GGNNCC 2 cut(s) 324, 600
BspPI GGATC 2 cut(s) 317, 330
BspQI GCTCTTC 1 cut(s) 281
BspT104I TTCGAA 1 cut(s) 270
BspTI CTTAAG 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 668
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 3 cut(s) 70, 372, 677
BsrI ACTGG 1 cut(s) 505
BssAI RCCGGY 3 cut(s) 70, 372, 677
BssECI CCNNGG 2 cut(s) 195, 696
BssMI GATC 4 cut(s) 279, 322, 364, 607
BssSI CACGAG 1 cut(s) 327
BssT1I CCWWGG 2 cut(s) 195, 696
Bst2BI CACGAG 1 cut(s) 327
Bst4CI ACNGT 3 cut(s) 389, 725, 761
Bst6I CTCTTC 1 cut(s) 281
BstAFI CTTAAG 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 164
BstDSI CCRYGG 2 cut(s) 195, 696
BstEII GGTNACC 2 cut(s) 199, 700
BstF5I GGATG 2 cut(s) 468, 748
BstFNI CGCG 3 cut(s) 231, 239, 261
BstHHI GCGC 2 cut(s) 239, 261
BstKTI GATC 4 cut(s) 282, 325, 367, 610
BstMAI GTCTC 2 cut(s) 152, 668
BstMBI GATC 4 cut(s) 279, 322, 364, 607
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 2 cut(s) 80, 308
BstPI GGTNACC 2 cut(s) 199, 700
BstSCI CCNGG 2 cut(s) 407, 765
BstSFI CTRYAG 1 cut(s) 760
BstUI CGCG 3 cut(s) 231, 239, 261
BstV1I GCAGC 2 cut(s) 86, 92
BstV2I GAAGAC 2 cut(s) 429, 660
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BstZI CGGCCG 1 cut(s) 405
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 2 cut(s) 155, 407
BtgI CCRYGG 2 cut(s) 195, 696
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 2 cut(s) 468, 748
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 72
CfoI GCGC 2 cut(s) 239, 261
Cfr10I RCCGGY 3 cut(s) 70, 372, 677
Cfr13I GGNCC 1 cut(s) 153
CseI GACGC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 370
CspAI ACCGGT 2 cut(s) 372, 677
CviAII CATG 2 cut(s) 196, 697
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 1 cut(s) 164
DpnI GATC 4 cut(s) 281, 324, 366, 609
DpnII GATC 4 cut(s) 279, 322, 364, 607
EaeI YGGCCR 1 cut(s) 405
EagI CGGCCG 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
EclXI CGGCCG 1 cut(s) 405
Eco130I CCWWGG 2 cut(s) 195, 696
Eco31I GGTCTC 1 cut(s) 668
Eco52I CGGCCG 1 cut(s) 405
Eco57I CTGAAG 1 cut(s) 423
Eco91I GGTNACC 2 cut(s) 199, 700
EcoO65I GGTNACC 2 cut(s) 199, 700
EcoT14I CCWWGG 2 cut(s) 195, 696
ErhI CCWWGG 2 cut(s) 195, 696
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 2 cut(s) 199, 700
FaiI YATR 8 cut(s) 197, 352, 520, 522, 625, 672, 698, 783
FaqI GGGAC 1 cut(s) 511
FatI CATG 2 cut(s) 195, 696
FauNDI CATATG 1 cut(s) 520
Fnu4HI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
FokI GGATG 2 cut(s) 455, 755
Fsp4HI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
FspBI CTAG 1 cut(s) 509
GlaI GCGC 2 cut(s) 238, 260
GluI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
GsaI CCCAGC 1 cut(s) 349
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 2 cut(s) 155, 407
HapII CCGG 5 cut(s) 71, 373, 408, 678, 766
HgaI GACGC 1 cut(s) 250
HhaI GCGC 2 cut(s) 239, 261
Hin1II CATG 2 cut(s) 199, 700
Hin6I GCGC 2 cut(s) 237, 259
HinP1I GCGC 2 cut(s) 237, 259
HinfI GANTC 2 cut(s) 160, 434
HpaII CCGG 5 cut(s) 71, 373, 408, 678, 766
HphI GGTGA 4 cut(s) 193, 256, 401, 685
Hpy188I TCNGA 1 cut(s) 538
Hpy188III TCNNGA 3 cut(s) 283, 399, 441
HpyAV CCTTC 2 cut(s) 277, 571
HpyCH4III ACNGT 3 cut(s) 389, 725, 761
HpyCH4IV ACGT 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 308
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 2 cut(s) 199, 700
HspAI GCGC 2 cut(s) 237, 259
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 4 cut(s) 279, 322, 364, 607
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 2 cut(s) 86, 92
LweI GCATC 2 cut(s) 92, 733
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 4 cut(s) 199, 262, 529, 700
MalI GATC 4 cut(s) 281, 324, 366, 609
MboI GATC 4 cut(s) 279, 322, 364, 607
MboII GAAGA 3 cut(s) 298, 429, 665
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 322
MluCI AATT 7 cut(s) 4, 103, 293, 340, 486, 491, 734
MlyI GAGTC 1 cut(s) 443
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 9 cut(s) 37, 166, 314, 336, 476, 497, 518, 589, 733
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 2 cut(s) 36, 489
MslI CAYNNNNRTG 1 cut(s) 538
MspA1I CMGCKG 2 cut(s) 77, 225
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 5 cut(s) 71, 373, 408, 678, 766
MspR9I CCNGG 2 cut(s) 409, 767
MunI CAATTG 1 cut(s) 340
Mva1269I GAATGC 2 cut(s) 545, 618
MvnI CGCG 3 cut(s) 231, 239, 261
MwoI GCNNNNNNNGC 2 cut(s) 80, 308
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 2 cut(s) 409, 767
NcoI CCATGG 2 cut(s) 195, 696
NdeI CATATG 1 cut(s) 520
NdeII GATC 4 cut(s) 279, 322, 364, 607
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 2 cut(s) 199, 700
NlaIV GGNNCC 2 cut(s) 324, 600
NmuCI GTSAC 2 cut(s) 199, 262
NspV TTCGAA 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 2 cut(s) 545, 618
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 160
PinAI ACCGGT 2 cut(s) 372, 677
PkrI GCNGC 6 cut(s) 76, 79, 82, 175, 211, 310
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PspEI GGTNACC 2 cut(s) 199, 700
PspFI CCCAGC 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 324, 600
PspPI GGNCC 1 cut(s) 153
PsuI RGATCY 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 225
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 538
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 2 cut(s) 36, 489
SatI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
Sau3AI GATC 4 cut(s) 279, 322, 364, 607
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 409, 767
SfaNI GCATC 2 cut(s) 92, 733
SfcI CTRYAG 1 cut(s) 760
SfuI TTCGAA 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 538
SmlI CTYRAG 3 cut(s) 28, 35, 544
SmoI CTYRAG 3 cut(s) 28, 35, 544
SpeI ACTAGT 1 cut(s) 508
Sse9I AATT 7 cut(s) 4, 103, 293, 340, 486, 491, 734
SsiI CCGC 5 cut(s) 77, 174, 210, 231, 309
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 2 cut(s) 407, 765
StyI CCWWGG 2 cut(s) 195, 696
TaaI ACNGT 3 cut(s) 389, 725, 761
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 158, 270, 575
TasI AATT 7 cut(s) 4, 103, 293, 340, 486, 491, 734
TauI GCSGC 4 cut(s) 80, 176, 212, 311
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 36, 489
Tru9I TTAA 2 cut(s) 36, 489
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 2 cut(s) 199, 262
TseI GCWGC 2 cut(s) 74, 80
Tsp45I GTSAC 2 cut(s) 199, 262
TspDTI ATGAA 3 cut(s) 17, 286, 410
TspGWI ACGGA 2 cut(s) 304, 461
TspRI CASTG 1 cut(s) 211
Vha464I CTTAAG 1 cut(s) 35
XapI RAATTY 2 cut(s) 4, 293
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.