Rh2DG234100

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003589
Physical Location & Seq
Reverse (-)
23271447 .. 23284171
12725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG234100.1

Sequence Viewer

Length: 576 bp
ATGGTCACCAGCAGCTGTCGTGGTGGCACTTATACCACCATCAAGGAGCACATCACCTTCGACAAGGAGATCAAGAAGAGCAAATTCATTGTCCTCGCCGCTTCCGTCTCCGATGAACAATCCGCTCTCTCCATCCTCTCTCAGGTGGGAGATCAGTACCGGTCTAATGATGATGGTGAGCCATCTGGAACGACCGGGAAACCCATTCATTCTGCTATTGAGTCTTCCGAAATTGATAGAGTAATGGTACTGGTCATCCGGTATTTTGGAGGAATTAAATTGGGGACTGAAGGACTAGTCAGGGCATATGGAGGAGTAACATCGGAATGCTTGAGAGATGCCCCAACTCATCCCGTAAAATCGAAGGTAAGAATGGGTGTAGAGGTTCCATTTGATCTTATCGGCATTCTATATCATCAGCTACAATCTTTTCAAGTTGAAGACATCAAACAAGATTATGAGACCGGTAAAGATGGTATCACCATGGTTACCTTTCAAGTTGATTTTGACCGTGTTGAGAAATTAGAGAATGCTATCAAAAACAACTGTAGCCGGGAGTTGGTGTTCTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

191

Amino Acids

21.24

Weight (kDa)

5.31

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 51 - 111 3.9e-20 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 125
AciI CCGC 2 cut(s) 99, 123
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 158, 249
AfiI CCNNNNNNNGG 2 cut(s) 142, 559
AgeI ACCGGT 2 cut(s) 159, 464
AgsI TTSAA 3 cut(s) 434, 440, 497
AhlI ACTAGT 1 cut(s) 295
AloI GAACNNNNNNTCC 1 cut(s) 548
AluBI AGCT 2 cut(s) 15, 421
AluI AGCT 2 cut(s) 15, 421
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 2 cut(s) 112, 455
AlwNI CAGNNNCTG 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 12
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AsiGI ACCGGT 2 cut(s) 159, 464
AsuC2I CCSGG 2 cut(s) 196, 554
AsuHPI GGTGA 3 cut(s) 46, 188, 472
BbsI GAAGAC 2 cut(s) 216, 447
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 24
BccI CCATC 5 cut(s) 47, 140, 167, 190, 467
BcnI CCSGG 2 cut(s) 196, 554
BcoDI GTCTC 2 cut(s) 112, 455
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 547
BisI GCNGC 2 cut(s) 13, 99
BlsI GCNGC 2 cut(s) 14, 100
Bme1390I CCNGG 2 cut(s) 196, 554
BmiI GGNNCC 1 cut(s) 387
BmrFI CCNGG 2 cut(s) 196, 554
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 2 cut(s) 216, 447
BpuEI CTTGAG 1 cut(s) 352
BpuMI CCSGG 2 cut(s) 196, 554
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 483
BsaWI WCCGGW 3 cut(s) 159, 258, 464
BsaXI ACNNNNNCTCC 1 cut(s) 548
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 559
Bse118I RCCGGY 2 cut(s) 159, 464
Bse1I ACTGG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 3 cut(s) 132, 255, 349
BseLI CCNNNNNNNGG 2 cut(s) 142, 559
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 255
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 195
BshTI ACCGGT 2 cut(s) 159, 464
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 5 cut(s) 160, 195, 259, 465, 553
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 2 cut(s) 142, 559
BsmAI GTCTC 2 cut(s) 112, 455
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 3 cut(s) 332, 405, 535
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 3 cut(s) 69, 151, 394
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 2 cut(s) 99, 123
BspCNI CTCAG 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 387
BspQI GCTCTTC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 455
BsrBI CCGCTC 1 cut(s) 125
BsrFI RCCGGY 2 cut(s) 159, 464
BsrI ACTGG 1 cut(s) 255
BssAI RCCGGY 2 cut(s) 159, 464
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 3 cut(s) 69, 151, 394
BssT1I CCWWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 512, 548
Bst6I CTCTTC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 141
BstDSI CCRYGG 1 cut(s) 483
BstEII GGTNACC 2 cut(s) 4, 487
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 132, 255, 349
BstKTI GATC 3 cut(s) 72, 154, 397
BstMAI GTCTC 2 cut(s) 112, 455
BstMBI GATC 3 cut(s) 69, 151, 394
BstMCI CGRYCG 1 cut(s) 195
BstPI GGTNACC 2 cut(s) 4, 487
BstSCI CCNGG 2 cut(s) 194, 552
BstSFI CTRYAG 1 cut(s) 547
BstV1I GCAGC 1 cut(s) 24
BstV2I GAAGAC 2 cut(s) 216, 447
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 3 cut(s) 132, 255, 349
CaiI CAGNNNCTG 1 cut(s) 15
Cfr10I RCCGGY 2 cut(s) 159, 464
Csp6I GTAC 2 cut(s) 157, 248
CspAI ACCGGT 2 cut(s) 159, 464
CviAII CATG 1 cut(s) 484
CviJI RGCY 4 cut(s) 15, 181, 421, 552
CviKI_1 RGCY 4 cut(s) 15, 181, 421, 552
CviQI GTAC 2 cut(s) 157, 248
DdeI CTNAG 1 cut(s) 141
DpnI GATC 3 cut(s) 71, 153, 396
DpnII GATC 3 cut(s) 69, 151, 394
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 309
Eco91I GGTNACC 2 cut(s) 4, 487
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO65I GGTNACC 2 cut(s) 4, 487
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 1 cut(s) 487
FaiI YATR 7 cut(s) 33, 307, 309, 412, 459, 485, 570
FaqI GGGAC 1 cut(s) 298
FatI CATG 1 cut(s) 483
FauNDI CATATG 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 13, 99
FokI GGATG 3 cut(s) 119, 242, 336
Fsp4HI GCNGC 2 cut(s) 13, 99
FspBI CTAG 1 cut(s) 296
GluI GCNGC 2 cut(s) 13, 99
HapII CCGG 5 cut(s) 160, 195, 259, 465, 553
Hin1II CATG 1 cut(s) 487
HinfI GANTC 1 cut(s) 221
HpaII CCGG 5 cut(s) 160, 195, 259, 465, 553
HphI GGTGA 3 cut(s) 46, 188, 472
Hpy188I TCNGA 3 cut(s) 112, 229, 325
Hpy188III TCNNGA 2 cut(s) 73, 186
HpyAV CCTTC 3 cut(s) 67, 284, 358
HpyCH4III ACNGT 2 cut(s) 512, 548
HpyF3I CTNAG 1 cut(s) 141
Hsp92II CATG 1 cut(s) 487
Kzo9I GATC 3 cut(s) 69, 151, 394
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 46
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 1 cut(s) 328
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 3 cut(s) 4, 316, 487
MalI GATC 3 cut(s) 71, 153, 396
MbiI CCGCTC 1 cut(s) 125
MboI GATC 3 cut(s) 69, 151, 394
MboII GAAGA 3 cut(s) 88, 216, 452
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 5 cut(s) 83, 231, 273, 278, 521
MlyI GAGTC 1 cut(s) 230
MnlI CCTC 5 cut(s) 104, 146, 263, 305, 376
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 325
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 5 cut(s) 160, 195, 259, 465, 553
MspR9I CCNGG 2 cut(s) 196, 554
Mva1269I GAATGC 3 cut(s) 332, 405, 535
NciI CCSGG 2 cut(s) 196, 554
NcoI CCATGG 1 cut(s) 483
NdeI CATATG 1 cut(s) 307
NdeII GATC 3 cut(s) 69, 151, 394
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 1 cut(s) 387
NmuCI GTSAC 1 cut(s) 4
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 3 cut(s) 332, 405, 535
PinAI ACCGGT 2 cut(s) 159, 464
PkrI GCNGC 2 cut(s) 14, 100
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PspEI GGTNACC 2 cut(s) 4, 487
PspN4I GGNNCC 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 15
RsaI GTAC 2 cut(s) 158, 249
RsaNI GTAC 2 cut(s) 157, 248
RseI CAYNNNNRTG 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 13, 99
Sau3AI GATC 3 cut(s) 69, 151, 394
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 196, 554
SduI GDGCHC 1 cut(s) 51
SetI ASST 7 cut(s) 17, 59, 147, 369, 387, 423, 494
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 547
SmiMI CAYNNNNRTG 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 5 cut(s) 83, 231, 273, 278, 521
SsiI CCGC 2 cut(s) 99, 123
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 194, 552
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 512, 548
TaqI TCGA 2 cut(s) 60, 362
TasI AATT 5 cut(s) 83, 231, 273, 278, 521
TauI GCSGC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 12
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 76, 129, 197
TspGWI ACGGA 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 83
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.