Rh2DG234300

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003589
Physical Location & Seq
Reverse (-)
23301786 .. 23303211
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG234300.1

Sequence Viewer

Length: 828 bp
ATGAATTCGGCTAATCCAAAACAAGCCTCAAGGCTTAAGCACGTTGATACGATAAAATCAAATCAAATGCCGGCAGCAGCAGCATCAATCACACCAATACCTAATTTCCATTTCCATTCCCACAAGGTAGCTCCCATTAGGTTTCGTCTCTGGGCCTCGATTCTCAGCATTGCCGCCGTAGCCAACCGAGCCACCATGGTCACCACTGCCGCCACCAGCAACAGCTGTCGCGGTGGCGCGTATACCACCATCAAAGAGCGCGTCACCTTCGAAAAAGATATCAAGAAGAGCAAATTCATCGCCCTCGCCGCTTCCGTCTCCGACGAACAATCCGCTTTCTCCTTCCTCTCTCAGGTTAGGGATCCTCGTGCTACTCACAATTGCTGGGCATACAAGGTGGGAGATCAGTACCGGTCTAATGATGATGGCGAGCCATCTGGAACGGCTGGGAAGCCCATTCATTCTGCTATTGAGTCTTCAGGAGTTGATAGAGTAATGGTGGTGGTCATCCGGTATTTTGGAGGAATTAAATTGGGCACTGGAGGACTAGTCAGGGCATATGGAGGAGTAACATCGGAATGCTTGAGAAATGCCCCAACTCATCTCGTAAAATCGAAGGTAAGAATGGGTGTAGAGGTTCCATTTGATCTTATCGGCATTCTATACCATCAGCTACAATCTTTTCAAGTTGAAGACATCAAACAAGATTATGAGACCGGTAAAGATGGTATCACCATGGTTACCTTTCAAGTTGATTTTGACTGTGTTGATAAATTAGAGGATGCTATCAAAAACAACTGTAGCCGGGAGTTAGTGTTCTATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

275

Amino Acids

30.24

Weight (kDa)

9.12

Isoelectric Point (pI)

28.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 95 - 195 9.8e-35 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 242
AccII CGCG 3 cut(s) 231, 239, 261
AciI CCGC 5 cut(s) 174, 210, 231, 309, 333
AclWI GGATC 2 cut(s) 356, 369
AcsI RAATTY 2 cut(s) 4, 293
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 352
AflII CTTAAG 1 cut(s) 35
AgeI ACCGGT 2 cut(s) 411, 716
AgsI TTSAA 3 cut(s) 686, 692, 749
AhlI ACTAGT 1 cut(s) 547
AloI GAACNNNNNNTCC 1 cut(s) 800
AluBI AGCT 3 cut(s) 131, 225, 673
AluI AGCT 3 cut(s) 131, 225, 673
Alw26I GTCTC 3 cut(s) 152, 322, 707
AlwI GGATC 2 cut(s) 356, 369
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 3 cut(s) 74, 77, 80
ApoI RAATTY 2 cut(s) 4, 293
ArsI GACNNNNNNTTYG 2 cut(s) 246, 278
AsiGI ACCGGT 2 cut(s) 411, 716
AspLEI GCGC 2 cut(s) 239, 261
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 3 cut(s) 193, 256, 724
AsuII TTCGAA 1 cut(s) 270
BaeGI GKGCMC 1 cut(s) 539
BamHI GGATCC 1 cut(s) 361
BauI CACGAG 1 cut(s) 366
BbsI GAAGAC 2 cut(s) 468, 699
BbvI GCAGC 3 cut(s) 86, 89, 92
BccI CCATC 5 cut(s) 257, 419, 442, 675, 719
BceAI ACGGC 2 cut(s) 161, 459
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 3 cut(s) 152, 322, 707
BcuI ACTAGT 1 cut(s) 547
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 799
BfrI CTTAAG 1 cut(s) 35
BisI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
BlsI GCNGC 6 cut(s) 76, 79, 82, 175, 211, 310
Bme1390I CCNGG 1 cut(s) 806
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 2 cut(s) 363, 639
BmrFI CCNGG 1 cut(s) 806
BmsI GCATC 2 cut(s) 92, 772
BpiI GAAGAC 2 cut(s) 468, 699
BpmI CTGGAG 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 13, 604
BpuMI CCSGG 1 cut(s) 806
BsaI GGTCTC 1 cut(s) 707
BsaJI CCNNGG 2 cut(s) 195, 735
BsaWI WCCGGW 3 cut(s) 411, 510, 716
BsaXI ACNNNNNCTCC 3 cut(s) 534, 564, 800
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse118I RCCGGY 3 cut(s) 70, 411, 716
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 195, 735
BseGI GGATG 2 cut(s) 507, 787
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 178, 365
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 3 cut(s) 86, 89, 92
BseYI CCCAGC 2 cut(s) 384, 446
Bsh1236I CGCG 3 cut(s) 231, 239, 261
BshFI GGCC 1 cut(s) 155
BshTI ACCGGT 2 cut(s) 411, 716
BsiSI CCGG 5 cut(s) 71, 412, 511, 717, 805
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 3 cut(s) 152, 322, 707
BsmBI CGTCTC 2 cut(s) 152, 322
BsmI GAATGC 2 cut(s) 584, 657
BsnI GGCC 1 cut(s) 155
Bso31I GGTCTC 1 cut(s) 707
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 3 cut(s) 361, 403, 646
Bsp19I CCATGG 2 cut(s) 195, 735
BspACI CCGC 5 cut(s) 174, 210, 231, 309, 333
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 2 cut(s) 177, 364
BspFNI CGCG 3 cut(s) 231, 239, 261
BspLI GGNNCC 2 cut(s) 363, 639
BspPI GGATC 2 cut(s) 356, 369
BspQI GCTCTTC 1 cut(s) 281
BspT104I TTCGAA 1 cut(s) 270
BspTI CTTAAG 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 707
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 3 cut(s) 70, 411, 716
BsrI ACTGG 1 cut(s) 544
BssAI RCCGGY 3 cut(s) 70, 411, 716
BssECI CCNNGG 2 cut(s) 195, 735
BssMI GATC 3 cut(s) 361, 403, 646
BssNAI GTATAC 1 cut(s) 243
BssSI CACGAG 1 cut(s) 366
BssT1I CCWWGG 2 cut(s) 195, 735
Bst1107I GTATAC 1 cut(s) 243
Bst2BI CACGAG 1 cut(s) 366
Bst4CI ACNGT 2 cut(s) 764, 800
Bst6I CTCTTC 1 cut(s) 281
BstAFI CTTAAG 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 72, 431
BstDEI CTNAG 2 cut(s) 164, 351
BstDSI CCRYGG 2 cut(s) 195, 735
BstEII GGTNACC 2 cut(s) 199, 739
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 507, 787
BstFNI CGCG 3 cut(s) 231, 239, 261
BstHHI GCGC 2 cut(s) 239, 261
BstKTI GATC 3 cut(s) 364, 406, 649
BstMAI GTCTC 3 cut(s) 152, 322, 707
BstMBI GATC 3 cut(s) 361, 403, 646
BstMWI GCNNNNNNNGC 4 cut(s) 80, 179, 188, 308
BstPI GGTNACC 2 cut(s) 199, 739
BstSCI CCNGG 1 cut(s) 804
BstSFI CTRYAG 1 cut(s) 799
BstSLI GKGCMC 1 cut(s) 539
BstUI CGCG 3 cut(s) 231, 239, 261
BstV1I GCAGC 3 cut(s) 86, 89, 92
BstV2I GAAGAC 2 cut(s) 468, 699
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BstZ17I GTATAC 1 cut(s) 243
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 2 cut(s) 195, 735
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 2 cut(s) 507, 787
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 204, 537
Cac8I GCNNGC 2 cut(s) 72, 431
CfoI GCGC 2 cut(s) 239, 261
Cfr10I RCCGGY 3 cut(s) 70, 411, 716
Cfr13I GGNCC 1 cut(s) 153
CseI GACGC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 409
CspAI ACCGGT 2 cut(s) 411, 716
CviAII CATG 2 cut(s) 196, 736
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 2 cut(s) 164, 351
DpnI GATC 3 cut(s) 363, 405, 648
DpnII GATC 3 cut(s) 361, 403, 646
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 195, 735
Eco31I GGTCTC 1 cut(s) 707
Eco32I GATATC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 462
Eco91I GGTNACC 2 cut(s) 199, 739
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoO65I GGTNACC 2 cut(s) 199, 739
EcoRI GAATTC 1 cut(s) 4
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 2 cut(s) 195, 735
ErhI CCWWGG 2 cut(s) 195, 735
Esp3I CGTCTC 2 cut(s) 152, 322
FaeI CATG 2 cut(s) 199, 739
FaiI YATR 9 cut(s) 197, 243, 391, 559, 561, 664, 711, 737, 822
FatI CATG 2 cut(s) 195, 735
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 1 cut(s) 242
Fnu4HI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
FokI GGATG 2 cut(s) 494, 794
Fsp4HI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
FspBI CTAG 1 cut(s) 548
GlaI GCGC 2 cut(s) 238, 260
GluI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
GsaI CCCAGC 2 cut(s) 388, 450
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 1 cut(s) 155
HapII CCGG 5 cut(s) 71, 412, 511, 717, 805
HgaI GACGC 1 cut(s) 250
HhaI GCGC 2 cut(s) 239, 261
Hin1II CATG 2 cut(s) 199, 739
Hin6I GCGC 2 cut(s) 237, 259
HinP1I GCGC 2 cut(s) 237, 259
HinfI GANTC 2 cut(s) 160, 473
HpaII CCGG 5 cut(s) 71, 412, 511, 717, 805
HphI GGTGA 3 cut(s) 193, 256, 724
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 2 cut(s) 322, 577
Hpy188III TCNNGA 3 cut(s) 283, 438, 480
Hpy8I GTNNAC 1 cut(s) 243
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 3 cut(s) 277, 352, 610
HpyCH4III ACNGT 2 cut(s) 764, 800
HpyCH4IV ACGT 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 179, 188, 308
HpyF3I CTNAG 2 cut(s) 164, 351
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 2 cut(s) 199, 739
HspAI GCGC 2 cut(s) 237, 259
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 361, 403, 646
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 3 cut(s) 86, 89, 92
LweI GCATC 2 cut(s) 92, 772
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 4 cut(s) 199, 262, 568, 739
MalI GATC 3 cut(s) 363, 405, 648
MboI GATC 3 cut(s) 361, 403, 646
MboII GAAGA 3 cut(s) 298, 468, 704
MfeI CAATTG 1 cut(s) 379
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 7 cut(s) 4, 103, 293, 379, 525, 530, 773
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 345
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 2 cut(s) 36, 528
MslI CAYNNNNRTG 1 cut(s) 577
MspA1I CMGCKG 1 cut(s) 225
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 5 cut(s) 71, 412, 511, 717, 805
MspR9I CCNGG 1 cut(s) 806
MunI CAATTG 1 cut(s) 379
Mva1269I GAATGC 2 cut(s) 584, 657
MvnI CGCG 3 cut(s) 231, 239, 261
MwoI GCNNNNNNNGC 4 cut(s) 80, 179, 188, 308
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 806
NcoI CCATGG 2 cut(s) 195, 735
NdeI CATATG 1 cut(s) 559
NdeII GATC 3 cut(s) 361, 403, 646
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 2 cut(s) 199, 739
NlaIV GGNNCC 2 cut(s) 363, 639
NmuCI GTSAC 2 cut(s) 199, 262
NspV TTCGAA 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 2 cut(s) 584, 657
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 160
PinAI ACCGGT 2 cut(s) 411, 716
PkrI GCNGC 6 cut(s) 76, 79, 82, 175, 211, 310
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PspEI GGTNACC 2 cut(s) 199, 739
PspFI CCCAGC 2 cut(s) 384, 446
PspN4I GGNNCC 2 cut(s) 363, 639
PspPI GGNCC 1 cut(s) 153
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 225
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 2 cut(s) 36, 528
SatI GCNGC 6 cut(s) 75, 78, 81, 174, 210, 309
Sau3AI GATC 3 cut(s) 361, 403, 646
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 806
SduI GDGCHC 1 cut(s) 539
SfaNI GCATC 2 cut(s) 92, 772
SfcI CTRYAG 1 cut(s) 799
SfuI TTCGAA 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 577
SmlI CTYRAG 3 cut(s) 28, 35, 583
SmoI CTYRAG 3 cut(s) 28, 35, 583
SpeI ACTAGT 1 cut(s) 547
Sse9I AATT 7 cut(s) 4, 103, 293, 379, 525, 530, 773
SsiI CCGC 5 cut(s) 174, 210, 231, 309, 333
SspMI CTAG 1 cut(s) 548
StyD4I CCNGG 1 cut(s) 804
StyI CCWWGG 2 cut(s) 195, 735
TaaI ACNGT 2 cut(s) 764, 800
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 158, 270, 614
TasI AATT 7 cut(s) 4, 103, 293, 379, 525, 530, 773
TauI GCSGC 3 cut(s) 176, 212, 311
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 36, 528
Tru9I TTAA 2 cut(s) 36, 528
TscAI CASTG 2 cut(s) 211, 544
TseFI GTSAC 2 cut(s) 199, 262
TseI GCWGC 3 cut(s) 74, 77, 80
Tsp45I GTSAC 2 cut(s) 199, 262
TspDTI ATGAA 3 cut(s) 17, 286, 449
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 2 cut(s) 211, 544
Vha464I CTTAAG 1 cut(s) 35
XagI CCTNNNNNAGG 1 cut(s) 350
XapI RAATTY 2 cut(s) 4, 293
XmiI GTMKAC 1 cut(s) 242
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.