Rh2CG230400

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003589
Physical Location & Seq
Reverse (-)
23310059 .. 23312561
2503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG230400.1

Sequence Viewer

Length: 681 bp
ATGAATTTGGCTAATCCAAAACAAGCCTCGAGGCTTAAGCACGTTGATACGATAAAATCAAATCAAATGCCGGCAGCAGCAGCATCAATCACACCAATACCTAATTTCCATTTCCATTCCCACAAGGTAGCTCCCATTAGGTTTCGTCTCTGGGCCTCGATTCTCAGCATTGCCGCCGTATCCAACCGAGCCACCATGGTCACCACCGCCACCAGCAGCAGCTGTCGCGGTGGCGCGTACACCACCATCAAAGAGCGCGTCACCTTCGAAAAAGAGATCAAGAAGAGCAAATTCATCGCCCTCGCCGCTTCCGTCTCCGACGAACAATCCGCTTTCTCCTTCCTCTCACAGGTTAGGGATCCTCGTGCTACTCACAATTGCTGGGCATACAAGGTGGGAGATCAGTACCGGTCTAATGATGACGGGGAGCCATCTGGAACGGCTGGGAAGCCCATTCATTCTGCTATTGAGTCTTCAGGAGTTGATAGAGTAATGGTGGTGGTCATCCGGTATTTTGGAGGAATTAAATTGGGGACTGGAGGACTAGTCAGGGCATATGGAGGAGTAACATCGGAATGCTTGAGAAATGCCCCAACTCATCTCGTAAAATCGAAGGTACTAACTCTCTTGCTCTGCTTTCGTGCTTTCATTTCAACTTGGGTGCCCAAAACTCTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

226

Amino Acids

24.6

Weight (kDa)

10.02

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 94 - 194 6.3e-35 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 661
AccII CGCG 3 cut(s) 228, 236, 258
AciI CCGC 5 cut(s) 174, 207, 228, 306, 330
AclWI GGATC 2 cut(s) 353, 366
AcsI RAATTY 2 cut(s) 4, 290
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 3 cut(s) 239, 407, 618
AfiI CCNNNNNNNGG 1 cut(s) 349
AflII CTTAAG 1 cut(s) 35
AgeI ACCGGT 1 cut(s) 408
AgsI TTSAA 1 cut(s) 654
AhlI ACTAGT 1 cut(s) 544
AluBI AGCT 2 cut(s) 131, 222
AluI AGCT 2 cut(s) 131, 222
Alw26I GTCTC 2 cut(s) 152, 319
AlwI GGATC 2 cut(s) 353, 366
AlwNI CAGNNNCTG 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 5 cut(s) 74, 77, 80, 216, 219
ApoI RAATTY 2 cut(s) 4, 290
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AsiGI ACCGGT 1 cut(s) 408
AspLEI GCGC 2 cut(s) 236, 258
AspS9I GGNCC 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 193, 253
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 666
BamHI GGATCC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 661
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 465
BbvI GCAGC 5 cut(s) 86, 89, 92, 228, 231
BccI CCATC 2 cut(s) 254, 439
BceAI ACGGC 2 cut(s) 161, 456
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciVI GTATCC 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 152, 319
BcuI ACTAGT 1 cut(s) 544
BfaI CTAG 1 cut(s) 545
BfrI CTTAAG 1 cut(s) 35
BfuI GTATCC 1 cut(s) 190
BisI GCNGC 7 cut(s) 75, 78, 81, 174, 217, 220, 306
BlsI GCNGC 7 cut(s) 76, 79, 82, 175, 218, 221, 307
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 3 cut(s) 360, 429, 663
BmsI GCATC 1 cut(s) 92
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 601
BsaJI CCNNGG 1 cut(s) 195
BsaWI WCCGGW 2 cut(s) 408, 507
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse118I RCCGGY 2 cut(s) 70, 408
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 1 cut(s) 666
BseXI GCAGC 5 cut(s) 86, 89, 92, 228, 231
BseYI CCCAGC 2 cut(s) 381, 443
Bsh1236I CGCG 3 cut(s) 228, 236, 258
BshFI GGCC 1 cut(s) 155
BshNI GGYRCC 1 cut(s) 661
BshTI ACCGGT 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 3 cut(s) 71, 409, 508
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 1 cut(s) 349
BsmAI GTCTC 2 cut(s) 152, 319
BsmBI CGTCTC 2 cut(s) 152, 319
BsmFI GGGAC 1 cut(s) 547
BsmI GAATGC 1 cut(s) 581
BsnI GGCC 1 cut(s) 155
BsoBI CYCGRG 1 cut(s) 28
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 3 cut(s) 276, 358, 400
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 5 cut(s) 174, 207, 228, 306, 330
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 177
BspFNI CGCG 3 cut(s) 228, 236, 258
BspLI GGNNCC 3 cut(s) 360, 429, 663
BspPI GGATC 2 cut(s) 353, 366
BspQI GCTCTTC 1 cut(s) 278
BspT104I TTCGAA 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 661
BspTI CTTAAG 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 2 cut(s) 70, 408
BsrI ACTGG 1 cut(s) 541
BssAI RCCGGY 2 cut(s) 70, 408
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 3 cut(s) 276, 358, 400
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 195
Bst2BI CACGAG 1 cut(s) 363
Bst6I CTCTTC 2 cut(s) 278, 678
BstAFI CTTAAG 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 164
BstDSI CCRYGG 1 cut(s) 195
BstEII GGTNACC 1 cut(s) 199
BstENI CCTNNNNNAGG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 504
BstFNI CGCG 3 cut(s) 228, 236, 258
BstHHI GCGC 2 cut(s) 236, 258
BstKTI GATC 3 cut(s) 279, 361, 403
BstMAI GTCTC 2 cut(s) 152, 319
BstMBI GATC 3 cut(s) 276, 358, 400
BstMWI GCNNNNNNNGC 3 cut(s) 80, 225, 305
BstPI GGTNACC 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 666
BstUI CGCG 3 cut(s) 228, 236, 258
BstV1I GCAGC 5 cut(s) 86, 89, 92, 228, 231
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 195
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 72
CaiI CAGNNNCTG 1 cut(s) 222
CfoI GCGC 2 cut(s) 236, 258
Cfr10I RCCGGY 2 cut(s) 70, 408
Cfr13I GGNCC 1 cut(s) 153
CseI GACGC 1 cut(s) 247
Csp6I GTAC 3 cut(s) 238, 406, 617
CspAI ACCGGT 1 cut(s) 408
CviAII CATG 1 cut(s) 196
CviQI GTAC 3 cut(s) 238, 406, 617
DdeI CTNAG 1 cut(s) 164
DpnI GATC 3 cut(s) 278, 360, 402
DpnII GATC 3 cut(s) 276, 358, 400
Eam1104I CTCTTC 2 cut(s) 278, 678
EarI CTCTTC 2 cut(s) 278, 678
Eco130I CCWWGG 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 28
Eco91I GGTNACC 1 cut(s) 199
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 195
Esp3I CGTCTC 2 cut(s) 152, 319
FaeI CATG 1 cut(s) 199
FaiI YATR 5 cut(s) 197, 388, 556, 558, 679
FaqI GGGAC 1 cut(s) 547
FatI CATG 1 cut(s) 195
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 7 cut(s) 75, 78, 81, 174, 217, 220, 306
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 7 cut(s) 75, 78, 81, 174, 217, 220, 306
FspBI CTAG 1 cut(s) 545
GlaI GCGC 2 cut(s) 235, 257
GluI GCNGC 7 cut(s) 75, 78, 81, 174, 217, 220, 306
GsaI CCCAGC 2 cut(s) 385, 447
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 1 cut(s) 155
HapII CCGG 3 cut(s) 71, 409, 508
HgaI GACGC 1 cut(s) 247
HhaI GCGC 2 cut(s) 236, 258
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 2 cut(s) 234, 256
HinP1I GCGC 2 cut(s) 234, 256
HinfI GANTC 2 cut(s) 160, 470
HpaII CCGG 3 cut(s) 71, 409, 508
HphI GGTGA 2 cut(s) 193, 253
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 2 cut(s) 319, 574
Hpy188III TCNNGA 3 cut(s) 280, 435, 477
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 3 cut(s) 274, 349, 607
HpyCH4IV ACGT 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 225, 305
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 2 cut(s) 234, 256
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 276, 358, 400
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 2 cut(s) 136, 427
Lsp1109I GCAGC 5 cut(s) 86, 89, 92, 228, 231
LweI GCATC 1 cut(s) 92
MaeI CTAG 1 cut(s) 545
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 3 cut(s) 199, 259, 565
MalI GATC 3 cut(s) 278, 360, 402
MboI GATC 3 cut(s) 276, 358, 400
MboII GAAGA 3 cut(s) 295, 465, 665
MfeI CAATTG 1 cut(s) 376
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 6 cut(s) 4, 103, 290, 376, 522, 527
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 2 cut(s) 207, 342
MnlI CCTC 9 cut(s) 24, 37, 166, 311, 353, 372, 512, 533, 554
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 2 cut(s) 36, 525
MslI CAYNNNNRTG 1 cut(s) 574
MspA1I CMGCKG 1 cut(s) 222
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 3 cut(s) 71, 409, 508
MunI CAATTG 1 cut(s) 376
Mva1269I GAATGC 1 cut(s) 581
MvnI CGCG 3 cut(s) 228, 236, 258
MwoI GCNNNNNNNGC 3 cut(s) 80, 225, 305
NaeI GCCGGC 1 cut(s) 72
NcoI CCATGG 1 cut(s) 195
NdeI CATATG 1 cut(s) 556
NdeII GATC 3 cut(s) 276, 358, 400
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 3 cut(s) 360, 429, 663
NmuCI GTSAC 2 cut(s) 199, 259
NspV TTCGAA 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 581
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 160
PinAI ACCGGT 1 cut(s) 408
PkrI GCNGC 7 cut(s) 76, 79, 82, 175, 218, 221, 307
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PspEI GGTNACC 1 cut(s) 199
PspFI CCCAGC 2 cut(s) 381, 443
PspN4I GGNNCC 3 cut(s) 360, 429, 663
PspPI GGNCC 1 cut(s) 153
PspXI VCTCGAGB 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 222
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 222
RsaI GTAC 3 cut(s) 239, 407, 618
RsaNI GTAC 3 cut(s) 238, 406, 617
RseI CAYNNNNRTG 1 cut(s) 574
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 36, 525
SatI GCNGC 7 cut(s) 75, 78, 81, 174, 217, 220, 306
Sau3AI GATC 3 cut(s) 276, 358, 400
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 479
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 1 cut(s) 92
Sfr274I CTCGAG 1 cut(s) 28
SfuI TTCGAA 1 cut(s) 267
SlaI CTCGAG 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 574
SmlI CTYRAG 3 cut(s) 28, 35, 580
SmoI CTYRAG 3 cut(s) 28, 35, 580
SpeI ACTAGT 1 cut(s) 544
Sse9I AATT 6 cut(s) 4, 103, 290, 376, 522, 527
SsiI CCGC 5 cut(s) 174, 207, 228, 306, 330
SspMI CTAG 1 cut(s) 545
StyI CCWWGG 1 cut(s) 195
TaiI ACGT 1 cut(s) 45
TaqI TCGA 4 cut(s) 29, 158, 267, 611
TasI AATT 6 cut(s) 4, 103, 290, 376, 522, 527
TauI GCSGC 2 cut(s) 176, 308
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 36, 525
Tru9I TTAA 2 cut(s) 36, 525
TseFI GTSAC 2 cut(s) 199, 259
TseI GCWGC 5 cut(s) 74, 77, 80, 216, 219
Tsp45I GTSAC 2 cut(s) 199, 259
TspDTI ATGAA 4 cut(s) 17, 283, 446, 637
TspGWI ACGGA 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 35
XagI CCTNNNNNAGG 1 cut(s) 347
XapI RAATTY 2 cut(s) 4, 290
XhoI CTCGAG 1 cut(s) 28
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.