Rroxscaffold_2G00132400

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003589
Physical Location & Seq
Forward (+)
69401989 .. 69403698
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132400.1

Sequence Viewer

Length: 825 bp
ATGAATTTGGCTAATCCAGAACAAGCGTCAAGGCTTAAGCTGGTTGATATCATAAAATCAAATCAAATGCCAGCAGCAGCAGCAGCAATCAGACCAATACCTAATTTCCATTTCCATTCCCACAAGGTAGCTCCCATTAGGTTTCGTCTCTGGGCCTCGATTCTCAGCATTGCCGCCGTATCCAACCGAGCCACCATGGTCACCACCGCCGCCAGCAGCAGCTGTCGCGGTGGCGCGTACACCACCATCAAAGAGCGCGTCACCTTCGAAAAAGAGATCAAGAAGAGCAAATTCATCGCCCTCGCCGCTTCCGTCTCCGACGAACAATCCGCCTTCTCCTTCCTCTCTCAGGTTAGGGATCCTCGTGCTACTCACAATTGCTGGGCGTACAAGGTGGGAGATCAGTACCGGTCTAATGATGACGGTGAGCCATCTGGAACGGCCGGGAAGCCCATTCATTCTGCTATTGAGTCTTCAGGAGTTGATAGAGTAATGGTGGTGGTCATCCGGTATTTTGGAGGAATTAAATTGGGGACTGGAGGACTGGTCAGGGCATATGGAGGAGTAACATCGGAATGCTTGAGAAATGCCCCAACTCATCTCGTAAAATCGAAGGTAAGAATGGGTGTAGAGGTTCCATTTGATCTTATCGGCATTCTATACCATCAGCTACAATCTTTTCAAGTTGAAGACATCAAACAAGATTATGAGACCGGTAAGGAAGGTATCACCATGGTTACCTTTCAAGTTGATTTTGACCATGTTGATAAATTAGAGGATGCTATCAAAAACAACTGTAGCCGGGAGTTGGTGTTCTATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

274

Amino Acids

30.24

Weight (kDa)

9.08

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 94 - 194 9.8e-35 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 228, 236, 258
AciI CCGC 6 cut(s) 174, 207, 210, 228, 306, 330
AclWI GGATC 2 cut(s) 353, 366
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 2 cut(s) 4, 290
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 3 cut(s) 239, 389, 407
AfiI CCNNNNNNNGG 2 cut(s) 349, 808
AflII CTTAAG 1 cut(s) 35
AgeI ACCGGT 2 cut(s) 408, 713
AgsI TTSAA 3 cut(s) 683, 689, 746
AloI GAACNNNNNNTCC 1 cut(s) 797
AluBI AGCT 4 cut(s) 40, 131, 222, 670
AluI AGCT 4 cut(s) 40, 131, 222, 670
Alw26I GTCTC 3 cut(s) 152, 319, 704
AlwI GGATC 2 cut(s) 353, 366
AlwNI CAGNNNCTG 1 cut(s) 222
AoxI GGCC 2 cut(s) 153, 441
ApeKI GCWGC 6 cut(s) 74, 77, 80, 83, 216, 219
ApoI RAATTY 2 cut(s) 4, 290
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AsiGI ACCGGT 2 cut(s) 408, 713
AspLEI GCGC 2 cut(s) 236, 258
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 2 cut(s) 445, 803
AsuHPI GGTGA 4 cut(s) 193, 253, 437, 721
AsuII TTCGAA 1 cut(s) 267
BamHI GGATCC 1 cut(s) 358
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 2 cut(s) 465, 696
BbvI GCAGC 6 cut(s) 86, 89, 92, 95, 228, 231
BccI CCATC 3 cut(s) 254, 439, 672
BceAI ACGGC 2 cut(s) 161, 456
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciVI GTATCC 1 cut(s) 190
BcnI CCSGG 2 cut(s) 445, 803
BcoDI GTCTC 3 cut(s) 152, 319, 704
BfmI CTRYAG 1 cut(s) 796
BfrI CTTAAG 1 cut(s) 35
BfuI GTATCC 1 cut(s) 190
BisI GCNGC 9 cut(s) 75, 78, 81, 84, 174, 210, 217, 220, 306
BlsI GCNGC 9 cut(s) 76, 79, 82, 85, 175, 211, 218, 221, 307
Bme1390I CCNGG 2 cut(s) 445, 803
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 2 cut(s) 360, 636
BmrFI CCNGG 2 cut(s) 445, 803
BmsI GCATC 1 cut(s) 769
BpiI GAAGAC 2 cut(s) 465, 696
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 601
BpuMI CCSGG 2 cut(s) 445, 803
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 2 cut(s) 195, 732
BsaWI WCCGGW 3 cut(s) 408, 507, 713
BsaXI ACNNNNNCTCC 3 cut(s) 531, 561, 797
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 808
Bse118I RCCGGY 2 cut(s) 408, 713
Bse1I ACTGG 2 cut(s) 541, 549
Bse3DI GCAATG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 195, 732
BseGI GGATG 2 cut(s) 504, 784
BseLI CCNNNNNNNGG 2 cut(s) 349, 808
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 178, 362
BseNI ACTGG 2 cut(s) 541, 549
BseRI GAGGAG 1 cut(s) 576
BseX3I CGGCCG 1 cut(s) 441
BseXI GCAGC 6 cut(s) 86, 89, 92, 95, 228, 231
BseYI CCCAGC 1 cut(s) 381
Bsh1236I CGCG 3 cut(s) 228, 236, 258
Bsh1285I CGRYCG 1 cut(s) 444
BshFI GGCC 2 cut(s) 155, 443
BshTI ACCGGT 2 cut(s) 408, 713
BsiEI CGRYCG 1 cut(s) 444
BsiSI CCGG 5 cut(s) 409, 444, 508, 714, 802
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 2 cut(s) 349, 808
BsmAI GTCTC 3 cut(s) 152, 319, 704
BsmBI CGTCTC 2 cut(s) 152, 319
BsmFI GGGAC 1 cut(s) 547
BsmI GAATGC 2 cut(s) 581, 654
BsnI GGCC 2 cut(s) 155, 443
Bso31I GGTCTC 1 cut(s) 704
Bsp119I TTCGAA 1 cut(s) 267
Bsp143I GATC 4 cut(s) 276, 358, 400, 643
Bsp19I CCATGG 2 cut(s) 195, 732
BspACI CCGC 6 cut(s) 174, 207, 210, 228, 306, 330
BspANI GGCC 2 cut(s) 155, 443
BspCNI CTCAG 2 cut(s) 177, 361
BspFNI CGCG 3 cut(s) 228, 236, 258
BspLI GGNNCC 2 cut(s) 360, 636
BspPI GGATC 2 cut(s) 353, 366
BspQI GCTCTTC 1 cut(s) 278
BspT104I TTCGAA 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 2 cut(s) 408, 713
BsrI ACTGG 2 cut(s) 541, 549
BssAI RCCGGY 2 cut(s) 408, 713
BssECI CCNNGG 2 cut(s) 195, 732
BssMI GATC 4 cut(s) 276, 358, 400, 643
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 2 cut(s) 195, 732
Bst2BI CACGAG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 425, 797
Bst6I CTCTTC 1 cut(s) 278
BstAFI CTTAAG 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 72, 214
BstDEI CTNAG 2 cut(s) 164, 348
BstDSI CCRYGG 2 cut(s) 195, 732
BstEII GGTNACC 2 cut(s) 199, 736
BstENI CCTNNNNNAGG 1 cut(s) 347
BstF5I GGATG 2 cut(s) 504, 784
BstFNI CGCG 3 cut(s) 228, 236, 258
BstHHI GCGC 2 cut(s) 236, 258
BstKTI GATC 4 cut(s) 279, 361, 403, 646
BstMAI GTCTC 3 cut(s) 152, 319, 704
BstMBI GATC 4 cut(s) 276, 358, 400, 643
BstMCI CGRYCG 1 cut(s) 444
BstMWI GCNNNNNNNGC 4 cut(s) 80, 83, 225, 305
BstPI GGTNACC 2 cut(s) 199, 736
BstSCI CCNGG 2 cut(s) 443, 801
BstSFI CTRYAG 1 cut(s) 796
BstUI CGCG 3 cut(s) 228, 236, 258
BstV1I GCAGC 6 cut(s) 86, 89, 92, 95, 228, 231
BstV2I GAAGAC 2 cut(s) 465, 696
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BstZI CGGCCG 1 cut(s) 441
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 2 cut(s) 155, 443
BtgI CCRYGG 2 cut(s) 195, 732
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 2 cut(s) 504, 784
Cac8I GCNNGC 2 cut(s) 72, 214
CaiI CAGNNNCTG 1 cut(s) 222
CfoI GCGC 2 cut(s) 236, 258
Cfr10I RCCGGY 2 cut(s) 408, 713
Cfr13I GGNCC 1 cut(s) 153
CseI GACGC 2 cut(s) 15, 247
Csp6I GTAC 3 cut(s) 238, 388, 406
CspAI ACCGGT 2 cut(s) 408, 713
CviAII CATG 3 cut(s) 196, 733, 761
CviQI GTAC 3 cut(s) 238, 388, 406
DdeI CTNAG 2 cut(s) 164, 348
DpnI GATC 4 cut(s) 278, 360, 402, 645
DpnII GATC 4 cut(s) 276, 358, 400, 643
EaeI YGGCCR 1 cut(s) 441
EagI CGGCCG 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
EciI GGCGGA 1 cut(s) 319
EclXI CGGCCG 1 cut(s) 441
Eco130I CCWWGG 2 cut(s) 195, 732
Eco31I GGTCTC 1 cut(s) 704
Eco32I GATATC 1 cut(s) 49
Eco52I CGGCCG 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 459
Eco91I GGTNACC 2 cut(s) 199, 736
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoO65I GGTNACC 2 cut(s) 199, 736
EcoRV GATATC 1 cut(s) 49
EcoT14I CCWWGG 2 cut(s) 195, 732
ErhI CCWWGG 2 cut(s) 195, 732
Esp3I CGTCTC 2 cut(s) 152, 319
FaeI CATG 3 cut(s) 199, 736, 764
FaiI YATR 9 cut(s) 53, 197, 556, 558, 661, 708, 734, 762, 819
FaqI GGGAC 1 cut(s) 547
FatI CATG 3 cut(s) 195, 732, 760
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 9 cut(s) 75, 78, 81, 84, 174, 210, 217, 220, 306
FokI GGATG 2 cut(s) 491, 791
Fsp4HI GCNGC 9 cut(s) 75, 78, 81, 84, 174, 210, 217, 220, 306
GlaI GCGC 2 cut(s) 235, 257
GluI GCNGC 9 cut(s) 75, 78, 81, 84, 174, 210, 217, 220, 306
GsaI CCCAGC 1 cut(s) 385
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 2 cut(s) 155, 443
HapII CCGG 5 cut(s) 409, 444, 508, 714, 802
HgaI GACGC 2 cut(s) 15, 247
HhaI GCGC 2 cut(s) 236, 258
Hin1II CATG 3 cut(s) 199, 736, 764
Hin6I GCGC 2 cut(s) 234, 256
HinP1I GCGC 2 cut(s) 234, 256
HinfI GANTC 2 cut(s) 160, 470
HpaII CCGG 5 cut(s) 409, 444, 508, 714, 802
HphI GGTGA 4 cut(s) 193, 253, 437, 721
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 3 cut(s) 92, 319, 574
Hpy188III TCNNGA 4 cut(s) 17, 280, 435, 477
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 5 cut(s) 274, 343, 349, 607, 716
HpyCH4III ACNGT 2 cut(s) 425, 797
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 83, 225, 305
HpyF3I CTNAG 2 cut(s) 164, 348
Hsp92II CATG 3 cut(s) 199, 736, 764
HspAI GCGC 2 cut(s) 234, 256
Kzo9I GATC 4 cut(s) 276, 358, 400, 643
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 6 cut(s) 86, 89, 92, 95, 228, 231
LweI GCATC 1 cut(s) 769
MaeIII GTNAC 4 cut(s) 199, 259, 565, 736
MalI GATC 4 cut(s) 278, 360, 402, 645
MboI GATC 4 cut(s) 276, 358, 400, 643
MboII GAAGA 3 cut(s) 295, 465, 701
MfeI CAATTG 1 cut(s) 376
MflI RGATCY 1 cut(s) 358
MluCI AATT 7 cut(s) 4, 103, 290, 376, 522, 527, 770
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 2 cut(s) 207, 342
MnlI CCTC 9 cut(s) 166, 311, 353, 372, 512, 533, 554, 625, 769
MseI TTAA 2 cut(s) 36, 525
MslI CAYNNNNRTG 1 cut(s) 574
MspA1I CMGCKG 1 cut(s) 222
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 5 cut(s) 409, 444, 508, 714, 802
MspR9I CCNGG 2 cut(s) 445, 803
MunI CAATTG 1 cut(s) 376
Mva1269I GAATGC 2 cut(s) 581, 654
MvnI CGCG 3 cut(s) 228, 236, 258
MwoI GCNNNNNNNGC 4 cut(s) 80, 83, 225, 305
NciI CCSGG 2 cut(s) 445, 803
NcoI CCATGG 2 cut(s) 195, 732
NdeI CATATG 1 cut(s) 556
NdeII GATC 4 cut(s) 276, 358, 400, 643
NlaIII CATG 3 cut(s) 199, 736, 764
NlaIV GGNNCC 2 cut(s) 360, 636
NmuCI GTSAC 2 cut(s) 199, 259
NspV TTCGAA 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 2 cut(s) 581, 654
PfeI GAWTC 1 cut(s) 160
PinAI ACCGGT 2 cut(s) 408, 713
PkrI GCNGC 9 cut(s) 76, 79, 82, 85, 175, 211, 218, 221, 307
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PspEI GGTNACC 2 cut(s) 199, 736
PspFI CCCAGC 1 cut(s) 381
PspN4I GGNNCC 2 cut(s) 360, 636
PspPI GGNCC 1 cut(s) 153
PstNI CAGNNNCTG 1 cut(s) 222
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 222
RsaI GTAC 3 cut(s) 239, 389, 407
RsaNI GTAC 3 cut(s) 238, 388, 406
RseI CAYNNNNRTG 1 cut(s) 574
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 36, 525
SatI GCNGC 9 cut(s) 75, 78, 81, 84, 174, 210, 217, 220, 306
Sau3AI GATC 4 cut(s) 276, 358, 400, 643
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 445, 803
SfaNI GCATC 1 cut(s) 769
SfcI CTRYAG 1 cut(s) 796
SfuI TTCGAA 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 574
SmlI CTYRAG 2 cut(s) 35, 580
SmoI CTYRAG 2 cut(s) 35, 580
Sse9I AATT 7 cut(s) 4, 103, 290, 376, 522, 527, 770
SsiI CCGC 6 cut(s) 174, 207, 210, 228, 306, 330
StyD4I CCNGG 2 cut(s) 443, 801
StyI CCWWGG 2 cut(s) 195, 732
TaaI ACNGT 2 cut(s) 425, 797
TaqI TCGA 3 cut(s) 158, 267, 611
TasI AATT 7 cut(s) 4, 103, 290, 376, 522, 527, 770
TauI GCSGC 3 cut(s) 176, 212, 308
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 36, 525
Tru9I TTAA 2 cut(s) 36, 525
TseFI GTSAC 2 cut(s) 199, 259
TseI GCWGC 6 cut(s) 74, 77, 80, 83, 216, 219
Tsp45I GTSAC 2 cut(s) 199, 259
TspDTI ATGAA 3 cut(s) 17, 283, 446
TspGWI ACGGA 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 35
XagI CCTNNNNNAGG 1 cut(s) 347
XapI RAATTY 2 cut(s) 4, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.