Rh2CG230200

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003589
Physical Location & Seq
Reverse (-)
23294163 .. 23294561
399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG230200.1

Sequence Viewer

Length: 264 bp
ATGGTCACCAGCAGCTCTCGCGGTGGCACTTATACCACCATCAAGGAGCACATCACCTTCGACAAGGAGATCAAGAAGAGCAAATTCATTGTCCTCGCCGCTTCCGTCTCCGATGAACAATCCGCTCTCTCCATCCTCTCTCAGGTGGGAGATCAGTACCGGTCTAATGATGATGGTGAGCCATCTGGAACGACCGGGAAACCCATTCATTCTGCTATTGAGTCTTCCGGAATTGATAGAGTAATGGTACTGGTCATCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

87

Amino Acids

9.37

Weight (kDa)

6.05

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 51 - 87 6.4e-08 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 125
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 227
AciI CCGC 3 cut(s) 21, 99, 123
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 2 cut(s) 158, 249
AfiI CCNNNNNNNGG 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 159
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 112
Aor13HI TCCGGA 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 12
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AsiGI ACCGGT 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 46, 188
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 24
BccI CCATC 4 cut(s) 47, 140, 167, 190
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 112
BisI GCNGC 2 cut(s) 13, 99
BlsI GCNGC 2 cut(s) 14, 100
Bme1390I CCNGG 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 196
BsaWI WCCGGW 3 cut(s) 159, 227, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 255
BseAI TCCGGA 1 cut(s) 227
BseGI GGATG 2 cut(s) 132, 255
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 255
BseXI GCAGC 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 195
BshTI ACCGGT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 4 cut(s) 160, 195, 228, 259
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 112
BsmBI CGTCTC 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 51
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 2 cut(s) 69, 151
BspACI CCGC 3 cut(s) 21, 99, 123
BspCNI CTCAG 1 cut(s) 154
BspEI TCCGGA 1 cut(s) 227
BspFNI CGCG 1 cut(s) 21
BspQI GCTCTTC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 159
BssMI GATC 2 cut(s) 69, 151
Bst6I CTCTTC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 141
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 2 cut(s) 132, 255
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 2 cut(s) 72, 154
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 2 cut(s) 69, 151
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 1 cut(s) 18
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 1 cut(s) 194
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 24
BstV2I GAAGAC 1 cut(s) 216
BtsCI GGATG 2 cut(s) 132, 255
Cfr10I RCCGGY 1 cut(s) 159
Csp6I GTAC 2 cut(s) 157, 248
CspAI ACCGGT 1 cut(s) 159
CviJI RGCY 2 cut(s) 15, 181
CviKI_1 RGCY 2 cut(s) 15, 181
CviQI GTAC 2 cut(s) 157, 248
DdeI CTNAG 1 cut(s) 141
DpnI GATC 2 cut(s) 71, 153
DpnII GATC 2 cut(s) 69, 151
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 4
Esp3I CGTCTC 1 cut(s) 112
FaiI YATR 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 13, 99
FokI GGATG 2 cut(s) 119, 242
Fsp4HI GCNGC 2 cut(s) 13, 99
GluI GCNGC 2 cut(s) 13, 99
HapII CCGG 4 cut(s) 160, 195, 228, 259
HinfI GANTC 1 cut(s) 221
HpaII CCGG 4 cut(s) 160, 195, 228, 259
HphI GGTGA 2 cut(s) 46, 188
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 3 cut(s) 73, 186, 228
HpyAV CCTTC 1 cut(s) 67
HpyF10VI GCNNNNNNNGC 1 cut(s) 18
HpyF3I CTNAG 1 cut(s) 141
Kpn2I TCCGGA 1 cut(s) 227
Kzo9I GATC 2 cut(s) 69, 151
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 7 cut(s) 22, 128, 171, 173, 208, 236, 241
Lsp1109I GCAGC 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 4
MalI GATC 2 cut(s) 71, 153
MbiI CCGCTC 1 cut(s) 125
MboI GATC 2 cut(s) 69, 151
MboII GAAGA 2 cut(s) 88, 216
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 2 cut(s) 83, 231
MlyI GAGTC 1 cut(s) 230
MnlI CCTC 2 cut(s) 104, 146
MroI TCCGGA 1 cut(s) 227
MspI CCGG 4 cut(s) 160, 195, 228, 259
MspR9I CCNGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 18
NciI CCSGG 1 cut(s) 196
NdeII GATC 2 cut(s) 69, 151
NmuCI GTSAC 1 cut(s) 4
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 2 cut(s) 14, 100
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PspEI GGTNACC 1 cut(s) 4
RsaI GTAC 2 cut(s) 158, 249
RsaNI GTAC 2 cut(s) 157, 248
SapI GCTCTTC 1 cut(s) 71
SatI GCNGC 2 cut(s) 13, 99
Sau3AI GATC 2 cut(s) 69, 151
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 196
SduI GDGCHC 1 cut(s) 51
SetI ASST 3 cut(s) 17, 59, 147
Sse9I AATT 2 cut(s) 83, 231
SsiI CCGC 3 cut(s) 21, 99, 123
StyD4I CCNGG 1 cut(s) 194
TaqI TCGA 1 cut(s) 60
TasI AATT 2 cut(s) 83, 231
TauI GCSGC 1 cut(s) 101
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 12
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 76, 129, 197
TspGWI ACGGA 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.