Rorug02G0173600

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003589
Physical Location & Seq
Reverse (-)
15791441 .. 15792535
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0173600.1

Sequence Viewer

Length: 579 bp
ATGTCACATTTCAGCTTTCTAGGGTTTCAGTACGGCTCATTCACACTGAGAAAGTCTCGCAATAAACCAGCGCCCAAATTGAGTACTAATGTCTCCAAAGAGAGACAGAGTTCTAGCGTGTCAAGGTCTTCATCCCAGCCAAAAATGGAGGAGATAAGATGGGTGTTTGATAAATTTGACACCAACAAAGACGGGAAGATAAGTCGGGAGGAGTACAAGTCGGCGTTGAAAACCCTCGACAAAGAAATGACAGAAGCTGAGATTGCCAAGACATTCAAGGCCCTTGATTCTGACGGAGACGGGTCCATAGGCTGGACGGAGTTTGTGGAGATGTTCAACATGGGAGCTGATGAGGCCAAGAAAGCTGACATTGAAGGCGCTTTTCGGGTGTTTGATTTGGATGGAAATGGGAAGATAAGCGCAGAGGAGTTGTCTCTTGTTCTCAAGAAGCTGGGAGAGAATTGCAGCCTTGGGGCTTGTAAGAAAATGGTGAAAGGGGTGGATGTTGATGGTGATGGTTTGATAGATATAAATGAGTTTACCAAAATGATGGGAAGCTTCAAGAAACCAAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

192

Amino Acids

21.34

Weight (kDa)

6.64

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARC_Ca_bdg PF10591 31 - 110 1.5e-06 Secreted protein acidic and rich in cysteine Ca binding region
EF-hand_7 PF13499 50 - 112 4.7e-18 EF-hand domain pair
EF-hand_1 PF00036 51 - 79 1.2e-08 EF hand domain
EF-hand_5 PF13202 55 - 74 2.1e-07 EF hand
EF-hand_6 PF13405 55 - 79 2.8e-06 EF-hand domain
EF-hand_8 PF13833 74 - 114 4e-07 EF-hand domain pair
EF-hand_7 PF13499 122 - 184 1.2e-11 EF-hand domain pair
EF-hand_1 PF00036 124 - 151 4.1e-07 EF hand domain
EF-hand_6 PF13405 124 - 152 1e-06 EF-hand domain
EF-hand_5 PF13202 127 - 146 9e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 312
AcsI RAATTY 1 cut(s) 173
AfaI GTAC 4 cut(s) 32, 85, 215, 574
AfiI CCNNNNNNNGG 1 cut(s) 312
AgsI TTSAA 5 cut(s) 229, 277, 337, 374, 562
AluBI AGCT 6 cut(s) 15, 257, 347, 365, 451, 558
AluI AGCT 6 cut(s) 15, 257, 347, 365, 451, 558
Alw26I GTCTC 5 cut(s) 60, 97, 97, 291, 438
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 2 cut(s) 279, 354
ApeKI GCWGC 1 cut(s) 465
ApoI RAATTY 1 cut(s) 173
AspLEI GCGC 3 cut(s) 73, 380, 422
AspS9I GGNCC 2 cut(s) 280, 303
AsuHPI GGTGA 2 cut(s) 502, 524
AvaII GGWCC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 477
BccI CCATC 5 cut(s) 153, 395, 503, 509, 544
BceAI ACGGC 1 cut(s) 49
BcoDI GTCTC 5 cut(s) 60, 97, 97, 291, 438
BfaI CTAG 2 cut(s) 20, 114
BfoI RGCGCY 2 cut(s) 74, 381
BisI GCNGC 1 cut(s) 466
BlsI GCNGC 1 cut(s) 467
BmcAI AGTACT 2 cut(s) 85, 574
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 2 cut(s) 280, 303
BmiI GGNNCC 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 40, 72
BpuEI CTTGAG 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 469
BseGI GGATG 3 cut(s) 131, 406, 508
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMII CTCAG 2 cut(s) 38, 249
BseRI GAGGAG 3 cut(s) 164, 224, 440
BseXI GCAGC 1 cut(s) 477
BseYI CCCAGC 2 cut(s) 135, 451
BshFI GGCC 2 cut(s) 281, 356
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 5 cut(s) 60, 97, 97, 291, 438
BsmBI CGTCTC 1 cut(s) 291
BsnI GGCC 2 cut(s) 281, 356
BspANI GGCC 2 cut(s) 281, 356
BspCNI CTCAG 2 cut(s) 39, 250
BspLI GGNNCC 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 469
BstDEI CTNAG 2 cut(s) 47, 258
BstF5I GGATG 3 cut(s) 131, 406, 508
BstH2I RGCGCY 2 cut(s) 74, 381
BstHHI GCGC 3 cut(s) 73, 380, 422
BstMAI GTCTC 5 cut(s) 60, 97, 97, 291, 438
BstMWI GCNNNNNNNGC 3 cut(s) 263, 353, 362
BstV1I GCAGC 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 550
BsuRI GGCC 2 cut(s) 281, 356
BtsCI GGATG 3 cut(s) 131, 406, 508
BtsIMutI CAGTG 1 cut(s) 44
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 3 cut(s) 73, 380, 422
Cfr13I GGNCC 2 cut(s) 280, 303
Csp6I GTAC 4 cut(s) 31, 84, 214, 573
CviAII CATG 1 cut(s) 340
CviQI GTAC 4 cut(s) 31, 84, 214, 573
DdeI CTNAG 2 cut(s) 47, 258
Eco130I CCWWGG 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 303
EcoO109I RGGNCCY 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 469
Esp3I CGTCTC 1 cut(s) 291
FaeI CATG 1 cut(s) 343
FaiI YATR 3 cut(s) 308, 341, 530
FatI CATG 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 466
FokI GGATG 3 cut(s) 118, 413, 515
Fsp4HI GCNGC 1 cut(s) 466
FspBI CTAG 2 cut(s) 20, 114
GlaI GCGC 3 cut(s) 72, 379, 421
GluI GCNGC 1 cut(s) 466
GsaI CCCAGC 2 cut(s) 139, 455
HaeII RGCGCY 2 cut(s) 74, 381
HaeIII GGCC 2 cut(s) 281, 356
HhaI GCGC 3 cut(s) 73, 380, 422
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 3 cut(s) 71, 378, 420
HinP1I GCGC 3 cut(s) 71, 378, 420
HindIII AAGCTT 1 cut(s) 556
HinfI GANTC 1 cut(s) 287
HphI GGTGA 2 cut(s) 502, 524
Hpy166II GTNNAC 1 cut(s) 540
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 3 cut(s) 206, 445, 562
Hpy8I GTNNAC 1 cut(s) 540
HpyAV CCTTC 1 cut(s) 368
HpyCH4V TGCA 1 cut(s) 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 353, 362
HpyF3I CTNAG 2 cut(s) 47, 258
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 3 cut(s) 71, 378, 420
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 4 cut(s) 81, 149, 298, 437
Lsp1109I GCAGC 1 cut(s) 477
MaeI CTAG 2 cut(s) 20, 114
MaeIII GTNAC 1 cut(s) 3
MboII GAAGA 3 cut(s) 120, 208, 424
MluCI AATT 3 cut(s) 77, 173, 460
MnlI CCTC 5 cut(s) 142, 202, 245, 346, 418
MwoI GCNNNNNNNGC 3 cut(s) 263, 353, 362
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 1 cut(s) 3
PfeI GAWTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 312
PkrI GCNGC 1 cut(s) 467
PspFI CCCAGC 2 cut(s) 135, 451
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 2 cut(s) 280, 303
PstNI CAGNNNCTG 1 cut(s) 257
RsaI GTAC 4 cut(s) 32, 85, 215, 574
RsaNI GTAC 4 cut(s) 31, 84, 214, 573
SatI GCNGC 1 cut(s) 466
Sau96I GGNCC 2 cut(s) 280, 303
ScaI AGTACT 2 cut(s) 85, 574
SetI ASST 7 cut(s) 17, 128, 259, 349, 367, 453, 560
SinI GGWCC 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 3 cut(s) 77, 173, 460
SspMI CTAG 2 cut(s) 20, 114
StyI CCWWGG 1 cut(s) 469
TaqI TCGA 1 cut(s) 237
TasI AATT 3 cut(s) 77, 173, 460
TatI WGTACW 3 cut(s) 83, 213, 572
TfiI GAWTC 1 cut(s) 287
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 465
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 2 cut(s) 309, 332
TspRI CASTG 1 cut(s) 51
Van91I CCANNNNNTGG 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 303
XapI RAATTY 1 cut(s) 173
XspI CTAG 2 cut(s) 20, 114
ZrmI AGTACT 2 cut(s) 85, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.