MD15G1314800.v1.1

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003589
Physical Location & Seq
Reverse (-)
31989275 .. 31991279
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1314800.v1.1.491

Sequence Viewer

Length: 804 bp
ATGCCAGTAGCAGCGATAACGCCACCTAATTTCAATCTCAGTTTCCAGAAATACTACCAGAAGGTAGCGCCGACCAAGCTCCACCAAGGCATTCGAATCTTGGTTTCAATTGCAGGAGTAGGCTGCTGCGTCGCCACCGGAGCCAATAAAGCCATGGTCACCACCGCCGCCGCAACCAGCAGCAATAGCAACAGGAGTGGTGGAGGCGGCGCGTATACGGGCATCAAGGAGATAGTGAGATTCGAAAAAGAGATCAAGAAGAGCAAATTCATCGCCGTCGCTGGTCCCATCTCCGACGAACAATCCGCTCAATCGTTCCTTTCCCAGGTACGAGAGCAACGCGCTACTCACAATTGTTGGGCGTACAAGGTTGGAGATCAATTCCGGTCTAATGATGACGGTGAACCTTCCGGAACGGCCGGAAAACCAATCCATTCTGCTATTGAATCTTCAGGAATTGACAGAGTTATGGTGGTTGTCATCAGATATTTTGGTGGTATTAAATTGGGAACTGGAGGACTCGTCAGGGCTTACGGAGGAGTTGCCTCTGAATGCTTGAAAAATGCCCCAACTTGTCTTGTGAAATCAAAGGTAAGAATGGGGGTCGAGGTTCCCTTTGATCTTTTGGGCATTCTATACCATCAGCTACAATCTTTTCACGTTGAAGACATCAAGCAAGATTATGAGACCGGTAAAGATGGTATCACCATGGTTACTTACCAAGTTGATTTTGACCGTATTGAGAAATTGGAGGAAGCTATCAAAAACAATTGTAGCCGAGAATTAGTGTTCTATAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

268

Amino Acids

29.06

Weight (kDa)

8.92

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 86 - 186 1.6e-35 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 215
AccII CGCG 2 cut(s) 212, 342
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 5 cut(s) 165, 168, 171, 207, 306
AcoI YGGCCR 1 cut(s) 417
AcsI RAATTY 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 2 cut(s) 330, 365
AfiI CCNNNNNNNGG 1 cut(s) 325
AgeI ACCGGT 1 cut(s) 689
AgsI TTSAA 5 cut(s) 34, 108, 446, 559, 665
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 3 cut(s) 79, 646, 758
AluI AGCT 3 cut(s) 79, 646, 758
Alw26I GTCTC 1 cut(s) 680
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 1 cut(s) 417
ApeKI GCWGC 4 cut(s) 11, 123, 126, 180
ApoI RAATTY 1 cut(s) 266
AsiGI ACCGGT 1 cut(s) 689
AspLEI GCGC 3 cut(s) 70, 212, 344
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 3 cut(s) 151, 413, 697
AsuII TTCGAA 2 cut(s) 94, 243
AvaII GGWCC 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 672
BbvI GCAGC 4 cut(s) 23, 110, 113, 192
BccI CCATC 3 cut(s) 296, 648, 692
BceAI ACGGC 2 cut(s) 260, 432
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 680
BfoI RGCGCY 1 cut(s) 71
BisI GCNGC 7 cut(s) 12, 124, 127, 168, 171, 181, 208
BlsI GCNGC 7 cut(s) 13, 125, 128, 169, 172, 182, 209
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 3 cut(s) 142, 286, 612
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 534
Bpu14I TTCGAA 2 cut(s) 94, 243
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 4 cut(s) 85, 153, 324, 708
BsaWI WCCGGW 4 cut(s) 137, 384, 410, 689
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse118I RCCGGY 1 cut(s) 689
Bse1I ACTGG 2 cut(s) 5, 517
BseAI TCCGGA 1 cut(s) 410
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 4 cut(s) 85, 153, 324, 708
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 52
BseNI ACTGG 2 cut(s) 5, 517
BseRI GAGGAG 1 cut(s) 552
BseX3I CGGCCG 1 cut(s) 417
BseXI GCAGC 4 cut(s) 23, 110, 113, 192
Bsh1236I CGCG 2 cut(s) 212, 342
Bsh1285I CGRYCG 1 cut(s) 420
BshFI GGCC 1 cut(s) 419
BshTI ACCGGT 1 cut(s) 689
BsiEI CGRYCG 1 cut(s) 420
BsiSI CCGG 5 cut(s) 138, 385, 411, 420, 690
BslFI GGGAC 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 1 cut(s) 680
BsmFI GGGAC 1 cut(s) 270
BsmI GAATGC 3 cut(s) 90, 557, 630
BsnI GGCC 1 cut(s) 419
Bso31I GGTCTC 1 cut(s) 680
Bsp119I TTCGAA 2 cut(s) 94, 243
Bsp13I TCCGGA 1 cut(s) 410
Bsp143I GATC 3 cut(s) 252, 376, 619
Bsp19I CCATGG 2 cut(s) 153, 708
BspACI CCGC 5 cut(s) 165, 168, 171, 207, 306
BspANI GGCC 1 cut(s) 419
BspCNI CTCAG 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 410
BspFNI CGCG 2 cut(s) 212, 342
BspLI GGNNCC 3 cut(s) 142, 286, 612
BspQI GCTCTTC 1 cut(s) 254
BspT104I TTCGAA 2 cut(s) 94, 243
BspTNI GGTCTC 1 cut(s) 680
BsrBI CCGCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 689
BsrI ACTGG 2 cut(s) 5, 517
BssAI RCCGGY 1 cut(s) 689
BssECI CCNNGG 4 cut(s) 85, 153, 324, 708
BssMI GATC 3 cut(s) 252, 376, 619
BssNAI GTATAC 1 cut(s) 216
BssT1I CCWWGG 3 cut(s) 85, 153, 708
Bst1107I GTATAC 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 401, 737
Bst6I CTCTTC 1 cut(s) 254
BstBI TTCGAA 2 cut(s) 94, 243
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 2 cut(s) 153, 708
BstEII GGTNACC 1 cut(s) 157
BstENI CCTNNNNNAGG 1 cut(s) 323
BstFNI CGCG 2 cut(s) 212, 342
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 3 cut(s) 70, 212, 344
BstKTI GATC 3 cut(s) 255, 379, 622
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 3 cut(s) 252, 376, 619
BstMCI CGRYCG 1 cut(s) 420
BstMWI GCNNNNNNNGC 4 cut(s) 76, 140, 149, 186
BstNI CCWGG 1 cut(s) 326
BstPI GGTNACC 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 2 cut(s) 212, 342
BstV1I GCAGC 4 cut(s) 23, 110, 113, 192
BstV2I GAAGAC 1 cut(s) 672
BstZ17I GTATAC 1 cut(s) 216
BstZI CGGCCG 1 cut(s) 417
BsuRI GGCC 1 cut(s) 419
BtgI CCRYGG 2 cut(s) 153, 708
BtgZI GCGATG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 799
CfoI GCGC 3 cut(s) 70, 212, 344
Cfr10I RCCGGY 1 cut(s) 689
Cfr13I GGNCC 1 cut(s) 284
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 329, 364
CspAI ACCGGT 1 cut(s) 689
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 2 cut(s) 154, 709
CviJI RGCY 9 cut(s) 79, 123, 143, 152, 419, 530, 646, 758, 777
CviKI_1 RGCY 9 cut(s) 79, 123, 143, 152, 419, 530, 646, 758, 777
CviQI GTAC 2 cut(s) 329, 364
DdeI CTNAG 1 cut(s) 38
DpnI GATC 3 cut(s) 254, 378, 621
DpnII GATC 3 cut(s) 252, 376, 619
EaeI YGGCCR 1 cut(s) 417
EagI CGGCCG 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
EclXI CGGCCG 1 cut(s) 417
Eco130I CCWWGG 3 cut(s) 85, 153, 708
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 1 cut(s) 284
Eco52I CGGCCG 1 cut(s) 417
Eco57I CTGAAG 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO65I GGTNACC 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 3 cut(s) 85, 153, 708
ErhI CCWWGG 3 cut(s) 85, 153, 708
FaeI CATG 2 cut(s) 157, 712
FaiI YATR 7 cut(s) 155, 216, 470, 637, 684, 710, 795
FaqI GGGAC 1 cut(s) 270
FatI CATG 2 cut(s) 153, 708
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 7 cut(s) 12, 124, 127, 168, 171, 181, 208
Fsp4HI GCNGC 7 cut(s) 12, 124, 127, 168, 171, 181, 208
GlaI GCGC 3 cut(s) 69, 211, 343
GluI GCNGC 7 cut(s) 12, 124, 127, 168, 171, 181, 208
GsuI CTGGAG 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 1 cut(s) 419
HapII CCGG 5 cut(s) 138, 385, 411, 420, 690
HgaI GACGC 1 cut(s) 118
HhaI GCGC 3 cut(s) 70, 212, 344
Hin1II CATG 2 cut(s) 157, 712
Hin6I GCGC 3 cut(s) 68, 210, 342
HinP1I GCGC 3 cut(s) 68, 210, 342
HinfI GANTC 4 cut(s) 96, 240, 446, 519
HpaII CCGG 5 cut(s) 138, 385, 411, 420, 690
HphI GGTGA 3 cut(s) 151, 413, 697
Hpy166II GTNNAC 2 cut(s) 216, 404
Hpy188I TCNGA 3 cut(s) 295, 485, 550
Hpy188III TCNNGA 4 cut(s) 46, 256, 411, 453
Hpy8I GTNNAC 2 cut(s) 216, 404
Hpy99I CGWCG 3 cut(s) 134, 281, 299
HpyAV CCTTC 2 cut(s) 55, 417
HpyCH4III ACNGT 2 cut(s) 401, 737
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 140, 149, 186
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 2 cut(s) 157, 712
HspAI GCGC 3 cut(s) 68, 210, 342
Kpn2I TCCGGA 1 cut(s) 410
Kzo9I GATC 3 cut(s) 252, 376, 619
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 2 cut(s) 84, 140
Lsp1109I GCAGC 4 cut(s) 23, 110, 113, 192
LweI GCATC 1 cut(s) 231
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 2 cut(s) 157, 712
MalI GATC 3 cut(s) 254, 378, 621
MbiI CCGCTC 1 cut(s) 308
MboI GATC 3 cut(s) 252, 376, 619
MboII GAAGA 3 cut(s) 271, 441, 677
MfeI CAATTG 3 cut(s) 108, 352, 769
MlyI GAGTC 1 cut(s) 513
MmeI TCCRAC 2 cut(s) 318, 352
MnlI CCTC 6 cut(s) 197, 509, 530, 556, 601, 745
MroI TCCGGA 1 cut(s) 410
MseI TTAA 1 cut(s) 501
MspI CCGG 5 cut(s) 138, 385, 411, 420, 690
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 3 cut(s) 108, 352, 769
Mva1269I GAATGC 3 cut(s) 90, 557, 630
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 2 cut(s) 212, 342
MwoI GCNNNNNNNGC 4 cut(s) 76, 140, 149, 186
NcoI CCATGG 2 cut(s) 153, 708
NdeII GATC 3 cut(s) 252, 376, 619
NlaIII CATG 2 cut(s) 157, 712
NlaIV GGNNCC 3 cut(s) 142, 286, 612
NmeAIII GCCGAG 1 cut(s) 803
NmuCI GTSAC 1 cut(s) 157
NspV TTCGAA 2 cut(s) 94, 243
PciSI GCTCTTC 1 cut(s) 254
PctI GAATGC 3 cut(s) 90, 557, 630
PfeI GAWTC 3 cut(s) 96, 240, 446
PinAI ACCGGT 1 cut(s) 689
PkrI GCNGC 7 cut(s) 13, 125, 128, 169, 172, 182, 209
PleI GAGTC 1 cut(s) 513
PpsI GAGTC 1 cut(s) 513
Psp6I CCWGG 1 cut(s) 324
PspEI GGTNACC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 142, 286, 612
PspPI GGNCC 1 cut(s) 284
RsaI GTAC 2 cut(s) 330, 365
RsaNI GTAC 2 cut(s) 329, 364
SapI GCTCTTC 1 cut(s) 254
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 7 cut(s) 12, 124, 127, 168, 171, 181, 208
Sau3AI GATC 3 cut(s) 252, 376, 619
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 1 cut(s) 513
ScrFI CCNGG 1 cut(s) 326
SfaNI GCATC 1 cut(s) 231
SfuI TTCGAA 2 cut(s) 94, 243
SinI GGWCC 1 cut(s) 284
SsiI CCGC 5 cut(s) 165, 168, 171, 207, 306
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 3 cut(s) 85, 153, 708
TaaI ACNGT 2 cut(s) 401, 737
TaiI ACGT 1 cut(s) 663
TaqI TCGA 3 cut(s) 94, 243, 606
TauI GCSGC 3 cut(s) 170, 173, 210
TfiI GAWTC 3 cut(s) 96, 240, 446
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 4 cut(s) 11, 123, 126, 180
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 259
TspGWI ACGGA 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 284
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 1 cut(s) 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmiI GTMKAC 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.