Rh2BG238500

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003589
Physical Location & Seq
Reverse (-)
23718852 .. 23779522
60671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG238500.1

Sequence Viewer

Length: 633 bp
ATGGTCACCACCGCCACCAGCAGCAACAGCTGTCGCGGTGGCGCGTACACCACCATCAAAGAGCGCGTCACCTTCGAAAAAGAGATCAAGAAGAGCAAATTCATCGCCCTCGCCGCTTCCGTCTCCGACGAACAATCCGCTTTCTCCTTCCTCTCTCAGGTTAGGGATCCTCGTGCTACTCACAATTGCTGGGCGTACAAGGTGGGAGATCAGTACCGGTCTAATGATGACGGTGAGCCATCTGGAACGGCCGGGAAGCCCATTCATTCCGCTATTGAGTCTTCAGGAGTTGATAGAGTAATGGTGGTGGTCATCCGCTATTTTGGAGGAATTAAATTGGGGACTGGAGGACTAGTCAGGGCATATGGAGGAGTAACATCGGAATGCTTGAGAAATGCCCCAACTCATCTCGTAAAATCGAAGGTAAGAATGGGTGTAGAGGTTCCATTTGATCTTATCGGCATTCTATACCATCAGCTACAATCTTTTCAAGTTGAAGACATCAAACAAGATTATGAGACCGGTAAAGATGGTATCACCATGGTTACCTTTCAAGTTGATTTTGACCGTGTTGAGAAATTAGAGGATGCTATCAAAAACAACTGTAGCCGGGAGTTGGTGTTCTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

210

Amino Acids

23.18

Weight (kDa)

6.44

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Impact_N PF01205 30 - 130 5.3e-35 Impact N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 36, 44, 66
AciI CCGC 6 cut(s) 12, 36, 114, 138, 270, 316
AclWI GGATC 2 cut(s) 161, 174
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 3 cut(s) 47, 197, 215
AfiI CCNNNNNNNGG 2 cut(s) 157, 616
AgeI ACCGGT 2 cut(s) 216, 521
AgsI TTSAA 3 cut(s) 491, 497, 554
AhlI ACTAGT 1 cut(s) 352
AloI GAACNNNNNNTCC 1 cut(s) 605
AluBI AGCT 2 cut(s) 30, 478
AluI AGCT 2 cut(s) 30, 478
Alw26I GTCTC 2 cut(s) 127, 512
AlwI GGATC 2 cut(s) 161, 174
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AsiGI ACCGGT 2 cut(s) 216, 521
AspLEI GCGC 2 cut(s) 44, 66
AsuC2I CCSGG 2 cut(s) 253, 611
AsuHPI GGTGA 3 cut(s) 61, 245, 529
AsuII TTCGAA 1 cut(s) 75
BamHI GGATCC 1 cut(s) 166
BauI CACGAG 1 cut(s) 171
BbsI GAAGAC 2 cut(s) 273, 504
BbvI GCAGC 1 cut(s) 33
BccI CCATC 4 cut(s) 62, 247, 480, 524
BceAI ACGGC 1 cut(s) 264
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BcnI CCSGG 2 cut(s) 253, 611
BcoDI GTCTC 2 cut(s) 127, 512
BcuI ACTAGT 1 cut(s) 352
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 604
BisI GCNGC 2 cut(s) 22, 114
BlsI GCNGC 2 cut(s) 23, 115
Bme1390I CCNGG 2 cut(s) 253, 611
BmiI GGNNCC 2 cut(s) 168, 444
BmrFI CCNGG 2 cut(s) 253, 611
BmsI GCATC 1 cut(s) 577
BpiI GAAGAC 2 cut(s) 273, 504
BpmI CTGGAG 1 cut(s) 366
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 409
BpuMI CCSGG 2 cut(s) 253, 611
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 1 cut(s) 540
BsaWI WCCGGW 2 cut(s) 216, 521
BsaXI ACNNNNNCTCC 3 cut(s) 339, 369, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 616
Bse118I RCCGGY 2 cut(s) 216, 521
Bse1I ACTGG 1 cut(s) 349
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 2 cut(s) 312, 592
BseLI CCNNNNNNNGG 2 cut(s) 157, 616
BseMII CTCAG 2 cut(s) 170, 618
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 1 cut(s) 384
BseX3I CGGCCG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 33
BseYI CCCAGC 1 cut(s) 189
Bsh1236I CGCG 3 cut(s) 36, 44, 66
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 1 cut(s) 251
BshTI ACCGGT 2 cut(s) 216, 521
BsiEI CGRYCG 1 cut(s) 252
BsiSI CCGG 4 cut(s) 217, 252, 522, 610
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 157, 616
BsmAI GTCTC 2 cut(s) 127, 512
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 2 cut(s) 389, 462
BsnI GGCC 1 cut(s) 251
Bso31I GGTCTC 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 75
Bsp143I GATC 4 cut(s) 84, 166, 208, 451
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 6 cut(s) 12, 36, 114, 138, 270, 316
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 2 cut(s) 169, 619
BspFNI CGCG 3 cut(s) 36, 44, 66
BspLI GGNNCC 2 cut(s) 168, 444
BspPI GGATC 2 cut(s) 161, 174
BspQI GCTCTTC 1 cut(s) 86
BspT104I TTCGAA 1 cut(s) 75
BspTNI GGTCTC 1 cut(s) 512
BsrFI RCCGGY 2 cut(s) 216, 521
BsrI ACTGG 1 cut(s) 349
BssAI RCCGGY 2 cut(s) 216, 521
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 4 cut(s) 84, 166, 208, 451
BssSI CACGAG 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 233, 569, 605
Bst6I CTCTTC 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 75
BstDEI CTNAG 2 cut(s) 156, 627
BstDSI CCRYGG 1 cut(s) 540
BstEII GGTNACC 2 cut(s) 4, 544
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 312, 592
BstFNI CGCG 3 cut(s) 36, 44, 66
BstHHI GCGC 2 cut(s) 44, 66
BstKTI GATC 4 cut(s) 87, 169, 211, 454
BstMAI GTCTC 2 cut(s) 127, 512
BstMBI GATC 4 cut(s) 84, 166, 208, 451
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 2 cut(s) 27, 113
BstPI GGTNACC 2 cut(s) 4, 544
BstSCI CCNGG 2 cut(s) 251, 609
BstSFI CTRYAG 1 cut(s) 604
BstUI CGCG 3 cut(s) 36, 44, 66
BstV1I GCAGC 1 cut(s) 33
BstV2I GAAGAC 2 cut(s) 273, 504
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BstZI CGGCCG 1 cut(s) 249
BsuRI GGCC 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 540
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 312, 592
CfoI GCGC 2 cut(s) 44, 66
Cfr10I RCCGGY 2 cut(s) 216, 521
CseI GACGC 1 cut(s) 55
Csp6I GTAC 3 cut(s) 46, 196, 214
CspAI ACCGGT 2 cut(s) 216, 521
CviAII CATG 1 cut(s) 541
CviJI RGCY 6 cut(s) 30, 238, 251, 259, 478, 609
CviKI_1 RGCY 6 cut(s) 30, 238, 251, 259, 478, 609
CviQI GTAC 3 cut(s) 46, 196, 214
DdeI CTNAG 2 cut(s) 156, 627
DpnI GATC 4 cut(s) 86, 168, 210, 453
DpnII GATC 4 cut(s) 84, 166, 208, 451
EaeI YGGCCR 1 cut(s) 249
EagI CGGCCG 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EclXI CGGCCG 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 540
Eco31I GGTCTC 1 cut(s) 512
Eco52I CGGCCG 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 267
Eco91I GGTNACC 2 cut(s) 4, 544
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoO65I GGTNACC 2 cut(s) 4, 544
EcoT14I CCWWGG 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 540
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 1 cut(s) 544
FaiI YATR 5 cut(s) 364, 366, 469, 516, 542
FaqI GGGAC 1 cut(s) 355
FatI CATG 1 cut(s) 540
FauNDI CATATG 1 cut(s) 364
Fnu4HI GCNGC 2 cut(s) 22, 114
FokI GGATG 2 cut(s) 299, 599
Fsp4HI GCNGC 2 cut(s) 22, 114
FspBI CTAG 1 cut(s) 353
GlaI GCGC 2 cut(s) 43, 65
GluI GCNGC 2 cut(s) 22, 114
GsaI CCCAGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 366
HaeIII GGCC 1 cut(s) 251
HapII CCGG 4 cut(s) 217, 252, 522, 610
HgaI GACGC 1 cut(s) 55
HhaI GCGC 2 cut(s) 44, 66
Hin1II CATG 1 cut(s) 544
Hin6I GCGC 2 cut(s) 42, 64
HinP1I GCGC 2 cut(s) 42, 64
HinfI GANTC 1 cut(s) 278
HpaII CCGG 4 cut(s) 217, 252, 522, 610
HphI GGTGA 3 cut(s) 61, 245, 529
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 3 cut(s) 127, 382, 628
Hpy188III TCNNGA 3 cut(s) 88, 243, 285
Hpy8I GTNNAC 1 cut(s) 48
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 3 cut(s) 82, 157, 415
HpyCH4III ACNGT 3 cut(s) 233, 569, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 113
HpyF3I CTNAG 2 cut(s) 156, 627
Hsp92II CATG 1 cut(s) 544
HspAI GCGC 2 cut(s) 42, 64
Kzo9I GATC 4 cut(s) 84, 166, 208, 451
LguI GCTCTTC 1 cut(s) 86
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 1 cut(s) 577
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 4 cut(s) 4, 67, 373, 544
MalI GATC 4 cut(s) 86, 168, 210, 453
MboI GATC 4 cut(s) 84, 166, 208, 451
MboII GAAGA 3 cut(s) 103, 273, 509
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 166
MluCI AATT 5 cut(s) 98, 184, 330, 335, 578
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 119, 161, 180, 320, 341, 362, 433, 577
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 4 cut(s) 217, 252, 522, 610
MspR9I CCNGG 2 cut(s) 253, 611
MunI CAATTG 1 cut(s) 184
Mva1269I GAATGC 2 cut(s) 389, 462
MvnI CGCG 3 cut(s) 36, 44, 66
MwoI GCNNNNNNNGC 2 cut(s) 27, 113
NciI CCSGG 2 cut(s) 253, 611
NcoI CCATGG 1 cut(s) 540
NdeI CATATG 1 cut(s) 364
NdeII GATC 4 cut(s) 84, 166, 208, 451
NlaIII CATG 1 cut(s) 544
NlaIV GGNNCC 2 cut(s) 168, 444
NmuCI GTSAC 2 cut(s) 4, 67
NspV TTCGAA 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 2 cut(s) 389, 462
PinAI ACCGGT 2 cut(s) 216, 521
PkrI GCNGC 2 cut(s) 23, 115
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PspEI GGTNACC 2 cut(s) 4, 544
PspFI CCCAGC 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 168, 444
PsuI RGATCY 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 3 cut(s) 47, 197, 215
RsaNI GTAC 3 cut(s) 46, 196, 214
RseI CAYNNNNRTG 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 2 cut(s) 22, 114
Sau3AI GATC 4 cut(s) 84, 166, 208, 451
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 253, 611
SetI ASST 8 cut(s) 32, 74, 162, 204, 426, 444, 480, 551
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 1 cut(s) 604
SfuI TTCGAA 1 cut(s) 75
SmiMI CAYNNNNRTG 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
SpeI ACTAGT 1 cut(s) 352
Sse9I AATT 5 cut(s) 98, 184, 330, 335, 578
SsiI CCGC 6 cut(s) 12, 36, 114, 138, 270, 316
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 2 cut(s) 251, 609
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 3 cut(s) 233, 569, 605
TaqI TCGA 2 cut(s) 75, 419
TasI AATT 5 cut(s) 98, 184, 330, 335, 578
TauI GCSGC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TseFI GTSAC 2 cut(s) 4, 67
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 2 cut(s) 4, 67
TspDTI ATGAA 2 cut(s) 91, 254
TspGWI ACGGA 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 155
XapI RAATTY 1 cut(s) 98
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.