FvH4_2g11353
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008928
Physical Location & Seq
Reverse (-)
9880463 .. 9882939
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11353.t1

Sequence Viewer

Length: 642 bp
ATGGGGAGAGGCAAGATTGTGATCCGAAGGATTGACAATTCCACGAGCAGGCAAGTGACGTTTTCCAAGCGAAGAAATGGGCTGCTGAAGAAGGCTAAGGAGCTTTCAATTCTCTGTGATGCTGAGGTTGGCTTGATCATCTTCTCCAGCACTGGCAGGCTCTATGATTATGCTAGTACTAGACAATTTTACCCTCACCACTTCAACATGTATAAGGAGAGAGAAAGTGGGAGTGAGAAGACTTTGCCGGTTTCTCGCTGGTCTCCGGTCACCGGCCGTCGGCTGCCGGACTCCGGTCCCCGGATTTTCTCCGACCGTCATCGGAATCTCGTCGGATTTTCTCCCGTCACCGGTCACTCCCCAAGCCACAAGAAATTTATTACCCCCAGTAATAAAAATTCCTCGACGCCTCCACGTCCTCCACCAGCAGTCGGTGCTACCAAATTAAGGTTGTTGAACGACGAGGCAGCGAAGGAGATAGAGTCGAACCAGAGGACAAGGATCTTGAGGCGGAGATCAGCGAGCTTTGACGACGAAGTACTAAAGGATCGTCGGGCTCGAGGACAAGGGAACCAAGGAGAAGCAGGAGGCGGTGAAGGTGATCGACGTCATCAGTGGCGCTCGATCGGTAGAGATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

214

Amino Acids

24.09

Weight (kDa)

10.99

Isoelectric Point (pI)

70.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 8.3e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 610
AciI CCGC 2 cut(s) 511, 591
AclWI GGATC 3 cut(s) 16, 509, 555
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 2 cut(s) 374, 397
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 2 cut(s) 407, 607
AfaI GTAC 2 cut(s) 178, 540
AfiI CCNNNNNNNGG 8 cut(s) 48, 272, 279, 293, 300, 350, 431, 447
AflIII ACRYGT 1 cut(s) 207
AgeI ACCGGT 1 cut(s) 350
AgsI TTSAA 3 cut(s) 108, 205, 457
AjiI CACGTC 1 cut(s) 416
AluBI AGCT 2 cut(s) 103, 525
AluI AGCT 2 cut(s) 103, 525
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 3 cut(s) 16, 509, 555
Ama87I CYCGRG 1 cut(s) 558
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 3 cut(s) 82, 283, 467
ApoI RAATTY 2 cut(s) 374, 397
AsiGI ACCGGT 1 cut(s) 350
AspLEI GCGC 1 cut(s) 621
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 5 cut(s) 188, 262, 340, 605, 611
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 559
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 245
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 3 cut(s) 69, 270, 479
BceAI ACGGC 1 cut(s) 261
BclI TGATCA 1 cut(s) 135
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 3 cut(s) 174, 180, 640
BfoI RGCGCY 1 cut(s) 622
BglII AGATCT 1 cut(s) 634
BisI GCNGC 3 cut(s) 83, 284, 468
BlsI GCNGC 3 cut(s) 84, 285, 469
BmcAI AGTACT 2 cut(s) 178, 540
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 558
BmgBI CACGTC 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 298, 572
BmrFI CCNGG 1 cut(s) 301
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 381
BoxI GACNNNNGTC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 245
BpmI CTGGAG 1 cut(s) 130
Bpu10I CCTNAGC 2 cut(s) 96, 123
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 2 cut(s) 407, 607
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 299, 574
BsaWI WCCGGW 3 cut(s) 265, 293, 350
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 8 cut(s) 48, 272, 279, 293, 300, 350, 431, 447
Bse118I RCCGGY 3 cut(s) 247, 272, 350
Bse1I ACTGG 2 cut(s) 157, 387
Bse8I GATNNNNATC 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 299, 574
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 8 cut(s) 48, 272, 279, 293, 300, 350, 431, 447
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 157, 387
BseX3I CGGCCG 1 cut(s) 274
BseXI GCAGC 3 cut(s) 69, 270, 479
Bsh1285I CGRYCG 3 cut(s) 277, 316, 627
BshFI GGCC 1 cut(s) 276
BshTI ACCGGT 1 cut(s) 350
BsiEI CGRYCG 3 cut(s) 277, 316, 627
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 7 cut(s) 248, 266, 273, 287, 294, 301, 351
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 8 cut(s) 48, 272, 279, 293, 300, 350, 431, 447
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 559
Bsp143I GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
BspACI CCGC 2 cut(s) 511, 591
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 298, 572
BspPI GGATC 3 cut(s) 16, 509, 555
BspTNI GGTCTC 1 cut(s) 267
BsrFI RCCGGY 3 cut(s) 247, 272, 350
BsrI ACTGG 2 cut(s) 157, 387
BssAI RCCGGY 3 cut(s) 247, 272, 350
BssECI CCNNGG 2 cut(s) 299, 574
BssMI GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
BssNI GRCGYC 2 cut(s) 407, 607
BssSI CACGAG 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 574
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 317
BstACI GRCGYC 2 cut(s) 407, 607
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 3 cut(s) 50, 158, 523
BstDEI CTNAG 2 cut(s) 96, 123
BstEII GGTNACC 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 622
BstHHI GCGC 1 cut(s) 621
BstKTI GATC 8 cut(s) 24, 138, 504, 518, 550, 604, 627, 637
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
BstMCI CGRYCG 3 cut(s) 277, 316, 627
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 211
BstPAI GACNNNNGTC 1 cut(s) 294
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 299
BstV1I GCAGC 3 cut(s) 69, 270, 479
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 2 cut(s) 501, 634
BstYI RGATCY 2 cut(s) 501, 634
BstZI CGGCCG 1 cut(s) 274
BsuRI GGCC 1 cut(s) 276
BtrI CACGTC 1 cut(s) 416
BtsIMutI CAGTG 2 cut(s) 150, 620
Cac8I GCNNGC 3 cut(s) 50, 158, 523
CfoI GCGC 1 cut(s) 621
Cfr10I RCCGGY 3 cut(s) 247, 272, 350
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 415
Csp6I GTAC 2 cut(s) 177, 539
CspAI ACCGGT 1 cut(s) 350
CviAII CATG 1 cut(s) 208
CviQI GTAC 2 cut(s) 177, 539
DdeI CTNAG 2 cut(s) 96, 123
DpnI GATC 8 cut(s) 23, 137, 503, 517, 549, 603, 626, 636
DpnII GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
EaeI YGGCCR 1 cut(s) 274
EagI CGGCCG 1 cut(s) 274
EciI GGCGGA 1 cut(s) 526
EclXI CGGCCG 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 559
Eco31I GGTCTC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 296
Eco52I CGGCCG 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 558
Eco91I GGTNACC 1 cut(s) 268
EcoO65I GGTNACC 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 1 cut(s) 211
FaiI YATR 4 cut(s) 165, 171, 209, 213
FaqI GGGAC 1 cut(s) 282
FatI CATG 1 cut(s) 207
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 83, 284, 468
FriOI GRGCYC 1 cut(s) 559
Fsp4HI GCNGC 3 cut(s) 83, 284, 468
FspBI CTAG 3 cut(s) 174, 180, 640
GlaI GCGC 1 cut(s) 620
GluI GCNGC 3 cut(s) 83, 284, 468
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 622
HaeIII GGCC 1 cut(s) 276
HapII CCGG 7 cut(s) 248, 266, 273, 287, 294, 301, 351
HgaI GACGC 1 cut(s) 415
HhaI GCGC 1 cut(s) 621
Hin1I GRCGYC 2 cut(s) 407, 607
Hin1II CATG 1 cut(s) 211
Hin6I GCGC 1 cut(s) 619
HinP1I GCGC 1 cut(s) 619
HinfI GANTC 3 cut(s) 290, 325, 482
HpaII CCGG 7 cut(s) 248, 266, 273, 287, 294, 301, 351
HphI GGTGA 5 cut(s) 188, 262, 340, 605, 611
Hpy188I TCNGA 4 cut(s) 26, 313, 324, 335
Hpy188III TCNNGA 1 cut(s) 505
Hpy99I CGWCG 7 cut(s) 282, 335, 409, 464, 536, 555, 609
HpyAV CCTTC 4 cut(s) 21, 85, 466, 590
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 3 cut(s) 59, 415, 607
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 2 cut(s) 96, 123
HpySE526I ACGT 3 cut(s) 59, 415, 607
Hsp92I GRCGYC 2 cut(s) 407, 607
Hsp92II CATG 1 cut(s) 211
HspAI GCGC 1 cut(s) 619
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 3 cut(s) 69, 270, 479
LweI GCATC 1 cut(s) 109
MaeI CTAG 3 cut(s) 174, 180, 640
MaeII ACGT 3 cut(s) 59, 415, 607
MaeIII GTNAC 4 cut(s) 55, 268, 346, 353
MalI GATC 8 cut(s) 23, 137, 503, 517, 549, 603, 626, 636
MboI GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
MboII GAAGA 4 cut(s) 84, 100, 133, 250
MflI RGATCY 2 cut(s) 501, 634
MhlI GDGCHC 1 cut(s) 559
MluCI AATT 6 cut(s) 37, 108, 185, 374, 397, 443
MlyI GAGTC 2 cut(s) 284, 491
MmeI TCCRAC 2 cut(s) 313, 336
MseI TTAA 1 cut(s) 446
MspI CCGG 7 cut(s) 248, 266, 273, 287, 294, 301, 351
MspR9I CCNGG 1 cut(s) 301
MwoI GCNNNNNNNGC 1 cut(s) 434
NciI CCSGG 1 cut(s) 301
NdeII GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
NlaIII CATG 1 cut(s) 211
NlaIV GGNNCC 2 cut(s) 298, 572
NmuCI GTSAC 4 cut(s) 55, 268, 346, 353
NspI RCATGY 1 cut(s) 211
PaeR7I CTCGAG 1 cut(s) 558
PciI ACATGT 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 556
PfeI GAWTC 1 cut(s) 325
PinAI ACCGGT 1 cut(s) 350
PkrI GCNGC 3 cut(s) 84, 285, 469
Ple19I CGATCG 1 cut(s) 627
PleI GAGTC 2 cut(s) 284, 490
PpsI GAGTC 2 cut(s) 284, 490
PscI ACATGT 1 cut(s) 207
PshAI GACNNNNGTC 1 cut(s) 294
PspEI GGTNACC 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 298, 572
PspPI GGNCC 1 cut(s) 296
PspXI VCTCGAGB 1 cut(s) 558
PsuI RGATCY 2 cut(s) 501, 634
PvuI CGATCG 1 cut(s) 627
RsaI GTAC 2 cut(s) 178, 540
RsaNI GTAC 2 cut(s) 177, 539
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 3 cut(s) 83, 284, 468
Sau3AI GATC 8 cut(s) 21, 135, 501, 515, 547, 601, 624, 634
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 2 cut(s) 178, 540
SchI GAGTC 2 cut(s) 284, 491
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 1 cut(s) 559
SetI ASST 8 cut(s) 62, 105, 129, 418, 452, 527, 601, 610
SfaNI GCATC 1 cut(s) 109
Sfr274I CTCGAG 1 cut(s) 558
SinI GGWCC 1 cut(s) 296
SlaI CTCGAG 1 cut(s) 558
SmlI CTYRAG 2 cut(s) 505, 558
SmoI CTYRAG 2 cut(s) 505, 558
Sse9I AATT 6 cut(s) 37, 108, 185, 374, 397, 443
SsiI CCGC 2 cut(s) 511, 591
SspMI CTAG 3 cut(s) 174, 180, 640
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 3 cut(s) 62, 418, 610
TaqI TCGA 5 cut(s) 404, 485, 559, 604, 623
TasI AATT 6 cut(s) 37, 108, 185, 374, 397, 443
TatI WGTACW 2 cut(s) 176, 538
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 2 cut(s) 157, 620
TseFI GTSAC 4 cut(s) 55, 268, 346, 353
TseI GCWGC 3 cut(s) 82, 283, 467
Tsp45I GTSAC 4 cut(s) 55, 268, 346, 353
TspRI CASTG 2 cut(s) 157, 620
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 2 cut(s) 374, 397
XceI RCATGY 1 cut(s) 211
XhoI CTCGAG 1 cut(s) 558
XspI CTAG 3 cut(s) 174, 180, 640
ZraI GACGTC 1 cut(s) 608
ZrmI AGTACT 2 cut(s) 178, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.