MD10G1306300.v1.1

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008928
Physical Location & Seq
Reverse (-)
39209841 .. 39216348
6508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1306300.v1.1.491

Sequence Viewer

Length: 315 bp
ATGATGAGGGAGAAGATCAAGATCAGGAAGATCGACTACTTGCGAGCAAGGCAGGTGACCTTCTCAAAGAGGAGAAGAGGGGATTTGAAGAAAGCTGGAGAGCTATGGATTCTGTGTGAATCTGAAGCTGTTGTTATCATCTTTTCCCAAACTGGCAAGCTCTTTAATTTCTCAAGCTCCAGGTACGGATTTTATGGGAGGCGTCTAGAAAGTTCAACTTTGATGAGTCGAAAGCACGGGATGCTAAGGATCGACTTGAAACAGTTGCCTCGCAGGCTCAAAAGGTCGTTTTCCGAAATAGAAGAGGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

105

Amino Acids

12.35

Weight (kDa)

10.78

Isoelectric Point (pI)

74.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 14 - 57 1.5e-17 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AclWI GGATC 1 cut(s) 257
AcuI CTGAAG 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 202
AfaI GTAC 1 cut(s) 185
AgsI TTSAA 3 cut(s) 88, 216, 259
AjnI CCWGG 1 cut(s) 179
AluBI AGCT 5 cut(s) 95, 103, 128, 160, 177
AluI AGCT 5 cut(s) 95, 103, 128, 160, 177
AlwI GGATC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BciT130I CCWGG 1 cut(s) 181
BfaI CTAG 1 cut(s) 206
BfuAI ACCTGC 1 cut(s) 43
BglI GCCNNNNNGGC 1 cut(s) 274
Bme1390I CCNGG 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 231
BpmI CTGGAG 2 cut(s) 117, 163
Bpu10I CCTNAGC 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 157
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 157
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 246
BseJI GATNNNNATC 1 cut(s) 20
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 85
Bsp143I GATC 4 cut(s) 15, 21, 30, 249
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 1 cut(s) 257
BsrI ACTGG 1 cut(s) 157
BssMI GATC 4 cut(s) 15, 21, 30, 249
BssNI GRCGYC 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 181
Bst4CI ACNGT 1 cut(s) 264
Bst6I CTCTTC 2 cut(s) 70, 297
BstACI GRCGYC 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 241
BstC8I GCNNGC 3 cut(s) 45, 158, 275
BstDEI CTNAG 1 cut(s) 245
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 1 cut(s) 246
BstKTI GATC 4 cut(s) 18, 24, 33, 252
BstMBI GATC 4 cut(s) 15, 21, 30, 249
BstMWI GCNNNNNNNGC 3 cut(s) 49, 241, 274
BstNI CCWGG 1 cut(s) 181
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 179
BtsCI GGATG 1 cut(s) 246
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 3 cut(s) 45, 158, 275
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 184
CviJI RGCY 6 cut(s) 95, 103, 128, 160, 177, 277
CviKI_1 RGCY 6 cut(s) 95, 103, 128, 160, 177, 277
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 1 cut(s) 245
DpnI GATC 4 cut(s) 17, 23, 32, 251
DpnII GATC 4 cut(s) 15, 21, 30, 249
Eam1104I CTCTTC 2 cut(s) 70, 297
EarI CTCTTC 2 cut(s) 70, 297
Eco57I CTGAAG 1 cut(s) 144
Eco91I GGTNACC 1 cut(s) 55
EcoO65I GGTNACC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 179
FaiI YATR 2 cut(s) 106, 195
FalI AAGNNNNNCTT 2 cut(s) 202, 234
FokI GGATG 1 cut(s) 253
FspBI CTAG 1 cut(s) 206
GsuI CTGGAG 2 cut(s) 117, 163
HgaI GACGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 202
HinfI GANTC 3 cut(s) 109, 119, 226
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 124, 295
Hpy188III TCNNGA 4 cut(s) 19, 25, 206, 312
HpyAV CCTTC 1 cut(s) 70
HpyCH4III ACNGT 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 241, 274
HpyF3I CTNAG 1 cut(s) 245
Hsp92I GRCGYC 1 cut(s) 202
Kzo9I GATC 4 cut(s) 15, 21, 30, 249
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 7 cut(s) 10, 38, 81, 138, 166, 193, 259
LweI GCATC 1 cut(s) 231
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 1 cut(s) 55
MalI GATC 4 cut(s) 17, 23, 32, 251
MboI GATC 4 cut(s) 15, 21, 30, 249
MboII GAAGA 5 cut(s) 25, 40, 87, 100, 314
MluCI AATT 1 cut(s) 166
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 5 cut(s) 63, 71, 192, 279, 298
MseI TTAA 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 181
MvaI CCWGG 1 cut(s) 181
MwoI GCNNNNNNNGC 3 cut(s) 49, 241, 274
NdeII GATC 4 cut(s) 15, 21, 30, 249
NmuCI GTSAC 1 cut(s) 55
PaqCI CACCTGC 1 cut(s) 43
PfeI GAWTC 2 cut(s) 109, 119
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 179
PspEI GGTNACC 1 cut(s) 55
PspGI CCWGG 1 cut(s) 179
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SaqAI TTAA 1 cut(s) 165
Sau3AI GATC 4 cut(s) 15, 21, 30, 249
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 181
SetI ASST 9 cut(s) 57, 62, 97, 105, 130, 162, 179, 185, 287
SfaNI GCATC 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
Sse9I AATT 1 cut(s) 166
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 33, 229, 252
TasI AATT 1 cut(s) 166
TfiI GAWTC 2 cut(s) 109, 119
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TseFI GTSAC 1 cut(s) 55
Tsp45I GTSAC 1 cut(s) 55
TspGWI ACGGA 1 cut(s) 201
XbaI TCTAGA 1 cut(s) 205
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.