MD09G1074000.v1.1

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008928
Physical Location & Seq
Forward (+)
5139651 .. 5140103
453 bp
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UTR
Exon/CDS
Intron
MD09G1074000.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGGAAGAGGTAAGGTGCAGATGAAGCGGATCGAGAACAAGATAAGCAGGCAAGTAACGTTTTCAAAGAGGAGGAGTGGATTGCTCAAAAAAGCTCATGAGATCTCTGTTCTGTGTGATGCTGAGGTGGCAGTTATTGTCTTCTCCACCGTAGGGAAGCTCTTTGAGTATTCTACTGATTTCAGGTCTGAACGTTACTCAAGCTTATTGAGCTTAGATTTTATCTGGGTATATACATCTCTTTTCTTTTTCTTGTTTGTTGTTAGTATTTTTTGCTTGTGCTACGCCAGTCATCTTAAACATAAAATAAACTTAAACTTAATTAAACAACTTATACACTTAAGCTCCATCTATCCCTCTACTTTTTTCTGGATCGGCCCTTTTTATCGATTTTTTGGTCCCATATTTAAGAGGTCGACATATTTACATGACAAAGATATACACAAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

151

Amino Acids

17.69

Weight (kDa)

9.85

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.7e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 416
AciI CCGC 1 cut(s) 28
AclI AACGTT 2 cut(s) 59, 193
AclWI GGATC 2 cut(s) 38, 380
AfiI CCNNNNNNNGG 1 cut(s) 153
AflII CTTAAG 1 cut(s) 340
AgsI TTSAA 1 cut(s) 66
AluBI AGCT 5 cut(s) 95, 160, 204, 213, 345
AluI AGCT 5 cut(s) 95, 160, 204, 213, 345
AlwI GGATC 2 cut(s) 38, 380
AoxI GGCC 1 cut(s) 376
AspS9I GGNCC 2 cut(s) 377, 398
AvaII GGWCC 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 133
BbvCI CCTCAGC 1 cut(s) 123
BccI CCATC 1 cut(s) 356
BfrI CTTAAG 1 cut(s) 340
BglII AGATCT 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 377, 398
BmiI GGNNCC 1 cut(s) 400
BmsI GCATC 1 cut(s) 109
BpiI GAAGAC 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 184
Bsa29I ATCGAT 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 288
BseCI ATCGAT 1 cut(s) 388
BseLI CCNNNNNNNGG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 288
BseRI GAGGAG 2 cut(s) 85, 88
BsgI GTGCAG 1 cut(s) 38
BshFI GGCC 1 cut(s) 378
BshVI ATCGAT 1 cut(s) 388
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 1 cut(s) 378
Bsp143I GATC 3 cut(s) 30, 102, 372
BspACI CCGC 1 cut(s) 28
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 1 cut(s) 115
BspDI ATCGAT 1 cut(s) 388
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 2 cut(s) 38, 380
BspTI CTTAAG 1 cut(s) 340
BsrI ACTGG 1 cut(s) 288
BssMI GATC 3 cut(s) 30, 102, 372
Bst4CI ACNGT 1 cut(s) 151
BstAFI CTTAAG 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 50
BstDEI CTNAG 2 cut(s) 123, 214
BstKTI GATC 3 cut(s) 33, 105, 375
BstMBI GATC 3 cut(s) 30, 102, 372
BstMWI GCNNNNNNNGC 3 cut(s) 25, 128, 210
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 388
BsuRI GGCC 1 cut(s) 378
BsuTUI ATCGAT 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 50
CciI TCATGA 1 cut(s) 97
Cfr13I GGNCC 2 cut(s) 377, 398
ClaI ATCGAT 1 cut(s) 388
CviAII CATG 2 cut(s) 98, 428
CviJI RGCY 6 cut(s) 95, 160, 204, 213, 345, 378
CviKI_1 RGCY 6 cut(s) 95, 160, 204, 213, 345, 378
DdeI CTNAG 2 cut(s) 123, 214
DpnI GATC 3 cut(s) 32, 104, 374
DpnII GATC 3 cut(s) 30, 102, 372
Eco47I GGWCC 1 cut(s) 398
FaeI CATG 2 cut(s) 101, 431
FaqI GGGAC 1 cut(s) 384
FatI CATG 2 cut(s) 97, 427
FblI GTMKAC 1 cut(s) 416
HaeIII GGCC 1 cut(s) 378
Hin1II CATG 2 cut(s) 101, 431
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 3 cut(s) 34, 98, 370
Hpy8I GTNNAC 1 cut(s) 417
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 2 cut(s) 59, 193
HpyCH4V TGCA 1 cut(s) 19
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 128, 210
HpyF3I CTNAG 2 cut(s) 123, 214
HpySE526I ACGT 2 cut(s) 59, 193
Hsp92II CATG 2 cut(s) 101, 431
Kzo9I GATC 3 cut(s) 30, 102, 372
LmnI GCTCC 1 cut(s) 350
LpnPI CCDG 5 cut(s) 34, 169, 211, 301, 355
LweI GCATC 1 cut(s) 109
MaeII ACGT 2 cut(s) 59, 193
MaeIII GTNAC 2 cut(s) 55, 194
MalI GATC 3 cut(s) 32, 104, 374
MboI GATC 3 cut(s) 30, 102, 372
MboII GAAGA 2 cut(s) 18, 133
MflI RGATCY 1 cut(s) 102
MluCI AATT 1 cut(s) 321
MnlI CCTC 5 cut(s) 63, 66, 118, 367, 405
MseI TTAA 6 cut(s) 297, 314, 320, 324, 341, 408
MspCI CTTAAG 1 cut(s) 340
MwoI GCNNNNNNNGC 3 cut(s) 25, 128, 210
NdeII GATC 3 cut(s) 30, 102, 372
NlaIII CATG 2 cut(s) 101, 431
NlaIV GGNNCC 1 cut(s) 400
PacI TTAATTAA 1 cut(s) 324
PagI TCATGA 1 cut(s) 97
Psp1406I AACGTT 2 cut(s) 59, 193
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 2 cut(s) 377, 398
PsuI RGATCY 1 cut(s) 102
SalI GTCGAC 1 cut(s) 415
SaqAI TTAA 6 cut(s) 297, 314, 320, 324, 341, 408
Sau3AI GATC 3 cut(s) 30, 102, 372
Sau96I GGNCC 2 cut(s) 377, 398
SfaNI GCATC 1 cut(s) 109
SinI GGWCC 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 199, 340
SmoI CTYRAG 2 cut(s) 199, 340
Sse9I AATT 1 cut(s) 321
SsiI CCGC 1 cut(s) 28
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 2 cut(s) 62, 196
TaqI TCGA 3 cut(s) 33, 388, 416
TasI AATT 1 cut(s) 321
Tru1I TTAA 6 cut(s) 297, 314, 320, 324, 341, 408
Tru9I TTAA 6 cut(s) 297, 314, 320, 324, 341, 408
TspDTI ATGAA 1 cut(s) 38
Vha464I CTTAAG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.