MD05G1049400.v1.1
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008928
Physical Location & Seq
Forward (+)
8584055 .. 8585473
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1049400.v1.1.491

Sequence Viewer

Length: 363 bp
ATGGGGAGAGGAAAGATTGTGATTAGAAGGATTGACAATTCGACGAGCAGGCAAGTGACCTTCTCGAAGAGAAGAAATGGGTTGCTTAAGAAGGCTAAGGAGCTATCAATCCTTTGTGATGCTGAGGTTGGATTGATTGTCTTCTCTAGCACCGGCAGGCTTTACGATTATGCTAGCACTAGGAAATGCTCTTTTCTTGGTACGTACGAAGGAGGATGCATGATGGGGAAGGCGGAGTTTTTAGATGTTAGAATTCTCTACCTACAAGTTCTCACTCAATTGCATAAGTATATATATAGGGGGCGGTTCTGTGGCGACATAAAAAAGACAAATTTTGAGACCGTTAGATTTGTACACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

121

Amino Acids

13.83

Weight (kDa)

9.93

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.7e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 233, 304
AcsI RAATTY 2 cut(s) 252, 331
AfaI GTAC 3 cut(s) 202, 206, 354
AflII CTTAAG 1 cut(s) 86
AluBI AGCT 1 cut(s) 103
AluI AGCT 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 332
ApoI RAATTY 2 cut(s) 252, 331
AsuNHI GCTAGC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 133
BbvCI CCTCAGC 1 cut(s) 123
BccI CCATC 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 3 cut(s) 147, 174, 180
BfrI CTTAAG 1 cut(s) 86
BmsI GCATC 2 cut(s) 109, 206
BmtI GCTAGC 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 133
Bpu10I CCTNAGC 2 cut(s) 96, 123
BsaAI YACGTR 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 332
Bse118I RCCGGY 1 cut(s) 152
BseGI GGATG 1 cut(s) 221
BseMII CTCAG 1 cut(s) 114
BsiSI CCGG 1 cut(s) 153
BsiWI CGTACG 1 cut(s) 204
BsmAI GTCTC 1 cut(s) 332
Bso31I GGTCTC 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 352
BspACI CCGC 2 cut(s) 233, 304
BspCNI CTCAG 1 cut(s) 115
BspOI GCTAGC 1 cut(s) 177
BspTI CTTAAG 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 343
Bst6I CTCTTC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 86
BstAUI TGTACA 1 cut(s) 352
BstBAI YACGTR 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 50, 158, 175
BstDEI CTNAG 2 cut(s) 96, 123
BstF5I GGATG 1 cut(s) 221
BstMAI GTCTC 1 cut(s) 332
BstSNI TACGTA 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 221
Cac8I GCNNGC 3 cut(s) 50, 158, 175
Cfr10I RCCGGY 1 cut(s) 152
Csp6I GTAC 3 cut(s) 201, 205, 353
CviAII CATG 1 cut(s) 220
CviJI RGCY 3 cut(s) 95, 103, 160
CviKI_1 RGCY 3 cut(s) 95, 103, 160
CviQI GTAC 3 cut(s) 201, 205, 353
DdeI CTNAG 2 cut(s) 96, 123
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 248
Eco105I TACGTA 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 252
EcoT22I ATGCAT 1 cut(s) 221
FaeI CATG 1 cut(s) 223
FaiI YATR 8 cut(s) 171, 221, 285, 291, 293, 295, 297, 320
FatI CATG 1 cut(s) 219
FokI GGATG 1 cut(s) 228
FspBI CTAG 3 cut(s) 147, 174, 180
HapII CCGG 1 cut(s) 153
Hin1II CATG 1 cut(s) 223
HpaII CCGG 1 cut(s) 153
Hpy166II GTNNAC 1 cut(s) 355
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 5 cut(s) 21, 70, 85, 203, 223
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 2 cut(s) 219, 283
HpyF3I CTNAG 2 cut(s) 96, 123
HpySE526I ACGT 1 cut(s) 203
Hsp92II CATG 1 cut(s) 223
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 3 cut(s) 34, 142, 166
LweI GCATC 2 cut(s) 109, 206
MaeI CTAG 3 cut(s) 147, 174, 180
MaeII ACGT 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 55
MboII GAAGA 3 cut(s) 79, 84, 133
MfeI CAATTG 1 cut(s) 278
MluCI AATT 4 cut(s) 37, 252, 278, 331
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 2 cut(s) 118, 206
Mph1103I ATGCAT 1 cut(s) 221
MseI TTAA 2 cut(s) 87, 361
MspCI CTTAAG 1 cut(s) 86
MspI CCGG 1 cut(s) 153
MunI CAATTG 1 cut(s) 278
NheI GCTAGC 1 cut(s) 173
NlaIII CATG 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 221
Pfl23II CGTACG 1 cut(s) 204
Ppu21I YACGTR 1 cut(s) 204
PspLI CGTACG 1 cut(s) 204
RsaI GTAC 3 cut(s) 202, 206, 354
RsaNI GTAC 3 cut(s) 201, 205, 353
SaqAI TTAA 2 cut(s) 87, 361
SetI ASST 5 cut(s) 62, 105, 129, 206, 264
SfaNI GCATC 2 cut(s) 109, 206
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SnaBI TACGTA 1 cut(s) 204
Sse9I AATT 4 cut(s) 37, 252, 278, 331
SsiI CCGC 2 cut(s) 233, 304
SspMI CTAG 3 cut(s) 147, 174, 180
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 1 cut(s) 206
TaqI TCGA 2 cut(s) 41, 65
TasI AATT 4 cut(s) 37, 252, 278, 331
TatI WGTACW 1 cut(s) 352
Tru1I TTAA 2 cut(s) 87, 361
Tru9I TTAA 2 cut(s) 87, 361
TseFI GTSAC 1 cut(s) 55
Tsp45I GTSAC 1 cut(s) 55
Vha464I CTTAAG 1 cut(s) 86
XapI RAATTY 2 cut(s) 252, 331
XspI CTAG 3 cut(s) 147, 174, 180
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.