FvH4_2g25870

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003757
Physical Location & Seq
Reverse (-)
20882047 .. 20884027
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25870.t1

Sequence Viewer

Length: 501 bp
ATGTCTCTCCCGGCCATTCTCGCGGTCCTCCTCCTCCTATCACCCTTATCCTTTTCCGCCGCCGATGACGACACACGGTCGGCCTACGACGTCATCCAGGACTTCAACTTCCCGATGGGGATTCTCCCCAAGGGCGTCACCGGCTACGACCTCGACCGGAGCAACGGCAAGTGGGCCGCCTACTTCGACGGCGCGTGCAGCTTCTCGCTCGAAGGCTCGTACCAGCTCAAGTACAAGTCCACCATCACCGGCACCATCTCCAACAACAGGCTCAAGGACCTGACCGGAGTCAGCGTTAAGGTCTTGTTCCTGTGGCTCAATATCGTCGAGGTTTCACGCAGCGGCGACGACCTGGACTTCTCGGTGGGGATCGCGTCGGCGTCGTTTCCGATTGATAATTTCTACGAGTGTCCGCAGTGCGGGTGTGGATTGAATTGCAGCCCGGCGCCGCCGCAAGTGAGGAAGATTAAGATGAAGAAGTCTCTGGTTTCTTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

167

Amino Acids

18.01

Weight (kDa)

5.33

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 134 6.2e-35 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 2 cut(s) 251, 445
AccII CGCG 3 cut(s) 23, 194, 374
AciI CCGC 9 cut(s) 23, 57, 60, 177, 342, 413, 420, 449, 452
AclWI GGATC 1 cut(s) 377
AcoI YGGCCR 1 cut(s) 12
AcyI GRCGYC 4 cut(s) 90, 135, 380, 446
AfaI GTAC 2 cut(s) 221, 233
AfiI CCNNNNNNNGG 2 cut(s) 267, 419
AgsI TTSAA 2 cut(s) 106, 433
AhdI GACNNNNNGTC 1 cut(s) 76
AjnI CCWGG 2 cut(s) 96, 351
AluBI AGCT 2 cut(s) 201, 226
AluI AGCT 2 cut(s) 201, 226
Alw26I GTCTC 2 cut(s) 9, 486
AlwI GGATC 1 cut(s) 377
AoxI GGCC 3 cut(s) 12, 81, 174
ApeKI GCWGC 3 cut(s) 198, 339, 438
AspLEI GCGC 2 cut(s) 194, 448
AspS9I GGNCC 3 cut(s) 25, 174, 277
AsuC2I CCSGG 2 cut(s) 11, 443
AsuHPI GGTGA 3 cut(s) 33, 130, 238
AvaII GGWCC 2 cut(s) 25, 277
BanI GGYRCC 2 cut(s) 251, 445
BbvI GCAGC 3 cut(s) 210, 351, 450
BccI CCATC 3 cut(s) 109, 251, 263
BceAI ACGGC 2 cut(s) 181, 205
BciT130I CCWGG 2 cut(s) 98, 353
BcnI CCSGG 2 cut(s) 11, 443
BcoDI GTCTC 2 cut(s) 9, 486
BfoI RGCGCY 1 cut(s) 449
BisI GCNGC 8 cut(s) 60, 177, 199, 340, 343, 439, 449, 452
BlsI GCNGC 8 cut(s) 61, 178, 200, 341, 344, 440, 450, 453
Bme1390I CCNGG 4 cut(s) 11, 98, 353, 443
Bme18I GGWCC 2 cut(s) 25, 277
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgT120I GGNCC 3 cut(s) 25, 174, 277
BmiI GGNNCC 2 cut(s) 253, 447
BmrFI CCNGG 4 cut(s) 11, 98, 353, 443
BoxI GACNNNNGTC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 212, 257
BpuMI CCSGG 2 cut(s) 11, 443
BsaHI GRCGYC 4 cut(s) 90, 135, 380, 446
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 2 cut(s) 156, 284
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 419
Bse118I RCCGGY 2 cut(s) 140, 248
BseBI CCWGG 2 cut(s) 98, 353
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 2 cut(s) 267, 419
BseRI GAGGAG 2 cut(s) 20, 23
BseXI GCAGC 3 cut(s) 210, 351, 450
BsgI GTGCAG 1 cut(s) 217
Bsh1236I CGCG 3 cut(s) 23, 194, 374
Bsh1285I CGRYCG 2 cut(s) 80, 157
BshFI GGCC 3 cut(s) 14, 83, 176
BshNI GGYRCC 2 cut(s) 251, 445
BsiEI CGRYCG 2 cut(s) 80, 157
BsiSI CCGG 6 cut(s) 11, 141, 157, 249, 285, 443
BslI CCNNNNNNNGG 2 cut(s) 267, 419
BsmAI GTCTC 2 cut(s) 9, 486
BsnI GGCC 3 cut(s) 14, 83, 176
Bsp143I GATC 1 cut(s) 369
BspACI CCGC 9 cut(s) 23, 57, 60, 177, 342, 413, 420, 449, 452
BspANI GGCC 3 cut(s) 14, 83, 176
BspFNI CGCG 3 cut(s) 23, 194, 374
BspLI GGNNCC 2 cut(s) 253, 447
BspPI GGATC 1 cut(s) 377
BspT107I GGYRCC 2 cut(s) 251, 445
BsrFI RCCGGY 2 cut(s) 140, 248
BssAI RCCGGY 2 cut(s) 140, 248
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 1 cut(s) 369
BssNI GRCGYC 4 cut(s) 90, 135, 380, 446
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 98, 353
Bst4CI ACNGT 1 cut(s) 78
BstACI GRCGYC 4 cut(s) 90, 135, 380, 446
BstC8I GCNNGC 1 cut(s) 196
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 3 cut(s) 23, 194, 374
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 2 cut(s) 194, 448
BstKTI GATC 1 cut(s) 372
BstMAI GTCTC 2 cut(s) 9, 486
BstMBI GATC 1 cut(s) 369
BstMCI CGRYCG 2 cut(s) 80, 157
BstMWI GCNNNNNNNGC 3 cut(s) 20, 141, 198
BstNI CCWGG 2 cut(s) 98, 353
BstPAI GACNNNNGTC 1 cut(s) 287
BstSCI CCNGG 4 cut(s) 9, 96, 351, 441
BstUI CGCG 3 cut(s) 23, 194, 374
BstV1I GCAGC 3 cut(s) 210, 351, 450
BsuRI GGCC 3 cut(s) 14, 83, 176
BtsCI GGATG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 2 cut(s) 194, 448
Cfr10I RCCGGY 2 cut(s) 140, 248
Cfr13I GGNCC 3 cut(s) 25, 174, 277
CseI GACGC 3 cut(s) 124, 363, 369
Csp6I GTAC 2 cut(s) 220, 232
CviQI GTAC 2 cut(s) 220, 232
DinI GGCGCC 1 cut(s) 447
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
DriI GACNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 12
Eam1105I GACNNNNNGTC 1 cut(s) 76
EciI GGCGGA 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 25, 277
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 96, 351
EcoT14I CCWWGG 1 cut(s) 129
EgeI GGCGCC 1 cut(s) 447
EheI GGCGCC 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 129
FauI CCCGC 1 cut(s) 413
Fnu4HI GCNGC 8 cut(s) 60, 177, 199, 340, 343, 439, 449, 452
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 8 cut(s) 60, 177, 199, 340, 343, 439, 449, 452
GlaI GCGC 2 cut(s) 193, 447
GluI GCNGC 8 cut(s) 60, 177, 199, 340, 343, 439, 449, 452
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 3 cut(s) 14, 83, 176
HapII CCGG 6 cut(s) 11, 141, 157, 249, 285, 443
HgaI GACGC 3 cut(s) 124, 363, 369
HhaI GCGC 2 cut(s) 194, 448
Hin1I GRCGYC 4 cut(s) 90, 135, 380, 446
Hin6I GCGC 2 cut(s) 192, 446
HinP1I GCGC 2 cut(s) 192, 446
HinfI GANTC 2 cut(s) 121, 288
HpaII CCGG 6 cut(s) 11, 141, 157, 249, 285, 443
HphI GGTGA 3 cut(s) 33, 130, 238
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 6 cut(s) 92, 191, 329, 350, 379, 385
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 2 cut(s) 198, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 141, 198
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 4 cut(s) 90, 135, 380, 446
HspAI GCGC 2 cut(s) 192, 446
KasI GGCGCC 1 cut(s) 445
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 159
Lsp1109I GCAGC 3 cut(s) 210, 351, 450
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 136
MalI GATC 1 cut(s) 371
MboI GATC 1 cut(s) 369
MboII GAAGA 3 cut(s) 475, 483, 487
MluCI AATT 2 cut(s) 397, 433
Mly113I GGCGCC 1 cut(s) 446
MlyI GAGTC 1 cut(s) 297
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 6 cut(s) 38, 41, 44, 161, 322, 453
MseI TTAA 2 cut(s) 297, 468
MspA1I CMGCKG 1 cut(s) 342
MspI CCGG 6 cut(s) 11, 141, 157, 249, 285, 443
MspR9I CCNGG 4 cut(s) 11, 98, 353, 443
MvaI CCWGG 2 cut(s) 98, 353
MvnI CGCG 3 cut(s) 23, 194, 374
MwoI GCNNNNNNNGC 3 cut(s) 20, 141, 198
NarI GGCGCC 1 cut(s) 446
NciI CCSGG 2 cut(s) 11, 443
NdeII GATC 1 cut(s) 369
NlaIV GGNNCC 2 cut(s) 253, 447
NmuCI GTSAC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 121
PfoI TCCNGGA 1 cut(s) 96
PkrI GCNGC 8 cut(s) 61, 178, 200, 341, 344, 440, 450, 453
PleI GAGTC 1 cut(s) 296
PluTI GGCGCC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 296
PpuMI RGGWCCY 1 cut(s) 277
PshAI GACNNNNGTC 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 96, 351
PspGI CCWGG 2 cut(s) 96, 351
PspN4I GGNNCC 2 cut(s) 253, 447
PspPI GGNCC 3 cut(s) 25, 174, 277
PspPPI RGGWCCY 1 cut(s) 277
RsaI GTAC 2 cut(s) 221, 233
RsaNI GTAC 2 cut(s) 220, 232
SaqAI TTAA 2 cut(s) 297, 468
SatI GCNGC 8 cut(s) 60, 177, 199, 340, 343, 439, 449, 452
Sau3AI GATC 1 cut(s) 369
Sau96I GGNCC 3 cut(s) 25, 174, 277
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 4 cut(s) 11, 98, 353, 443
SetI ASST 8 cut(s) 93, 153, 203, 228, 282, 303, 333, 354
SfoI GGCGCC 1 cut(s) 447
SinI GGWCC 2 cut(s) 25, 277
SmlI CTYRAG 2 cut(s) 227, 272
SmoI CTYRAG 2 cut(s) 227, 272
Sse9I AATT 2 cut(s) 397, 433
SsiI CCGC 9 cut(s) 23, 57, 60, 177, 342, 413, 420, 449, 452
SspDI GGCGCC 1 cut(s) 445
StyD4I CCNGG 4 cut(s) 9, 96, 351, 441
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 1 cut(s) 93
TaqI TCGA 4 cut(s) 153, 186, 210, 327
TasI AATT 2 cut(s) 397, 433
TatI WGTACW 1 cut(s) 231
TauI GCSGC 5 cut(s) 62, 179, 345, 451, 454
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 2 cut(s) 297, 468
Tru9I TTAA 2 cut(s) 297, 468
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 3 cut(s) 198, 339, 438
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 488
TspRI CASTG 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 25, 277
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.