MD11G1308200.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003757
Physical Location & Seq
Forward (+)
42202405 .. 42202896
492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1308200.v1.1.491

Sequence Viewer

Length: 492 bp
ATGTCTCCAAAGGCAACGCTGCTACCAGGCCTAATTCTCTTCTTTCTGATCTCCATCTCAATCCCGTCGTCCTTCGCCGCCGACGTGCTGACGGTTTACGAAGCCCTCGAGGAGTACGACTTCCCGGTCGGCATTCTCCCCAAAGGCGCACTGAGCTACAAGCTGGACAGCTCCACAGGTAAGTTCTCAGTCTACTTGAATAGCACTTGTACATTTTCCATCGACTCGTATAAGTTGAAGTACAAGTCCACCATAAAGGGTGTGATAAAGACCGACAAGATCTCAAGCTTGAGTGGCATCCAAGTTAAGGTGCTCTTTCTGTGGCTGAGCATTACCACCGTGACTCGTGACGACGATGAGCTAAAATTCTCGGTCGGCATCGGCTCCGCAAACTTTCCGGTCAGTAATTTCTATGAGAGTCCGACCTGTGGTTGCGGTTTCGATTGTGTCAACGGGAAAAGAAAGATTAAGAATTTGGTGTCCGCACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

164

Amino Acids

17.77

Weight (kDa)

8.61

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 137 1.1e-34 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AbsI CCTCGAGG 1 cut(s) 107
AccI GTMKAC 1 cut(s) 192
AciI CCGC 4 cut(s) 78, 387, 435, 483
AcsI RAATTY 2 cut(s) 365, 472
AfaI GTAC 3 cut(s) 116, 211, 242
AfiI CCNNNNNNNGG 2 cut(s) 307, 428
AgsI TTSAA 2 cut(s) 199, 238
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 5 cut(s) 156, 163, 171, 288, 361
AluI AGCT 5 cut(s) 156, 163, 171, 288, 361
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 19
ApoI RAATTY 2 cut(s) 365, 472
AspLEI GCGC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 107
BauI CACGAG 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 6
BccI CCATC 2 cut(s) 62, 227
BciT130I CCWGG 1 cut(s) 27
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 9
BglII AGATCT 1 cut(s) 279
BisI GCNGC 2 cut(s) 20, 78
BlpI GCTNAGC 1 cut(s) 326
BlsI GCNGC 2 cut(s) 21, 79
Bme1390I CCNGG 2 cut(s) 27, 125
BmeT110I CYCGRG 1 cut(s) 107
BmgBI CACGTC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 2 cut(s) 27, 125
BmsI GCATC 2 cut(s) 306, 387
Bpu1102I GCTNAGC 1 cut(s) 326
BpuEI CTTGAG 2 cut(s) 268, 310
BpuMI CCSGG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 53
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 428
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 297
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 2 cut(s) 307, 428
BseMII CTCAG 3 cut(s) 143, 201, 317
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 6
Bsh1285I CGRYCG 2 cut(s) 129, 375
BshFI GGCC 1 cut(s) 30
BsiEI CGRYCG 2 cut(s) 129, 375
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 125, 398
BslI CCNNNNNNNGG 2 cut(s) 307, 428
BsmAI GTCTC 1 cut(s) 9
BsmI GAATGC 1 cut(s) 132
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 209
Bsp143I GATC 2 cut(s) 48, 279
Bsp1720I GCTNAGC 1 cut(s) 326
BspACI CCGC 4 cut(s) 78, 387, 435, 483
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 3 cut(s) 144, 200, 318
BspLI GGNNCC 1 cut(s) 385
BsrGI TGTACA 1 cut(s) 209
BssMI GATC 2 cut(s) 48, 279
BssSI CACGAG 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 94, 340
Bst6I CTCTTC 1 cut(s) 44
BstAUI TGTACA 1 cut(s) 209
BstDEI CTNAG 3 cut(s) 152, 187, 326
BstF5I GGATG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 2 cut(s) 51, 282
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 48, 279
BstMCI CGRYCG 2 cut(s) 129, 375
BstMWI GCNNNNNNNGC 2 cut(s) 153, 294
BstNI CCWGG 1 cut(s) 27
BstSCI CCNGG 2 cut(s) 25, 123
BstV1I GCAGC 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BsuRI GGCC 1 cut(s) 30
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 149
CfoI GCGC 1 cut(s) 149
Csp6I GTAC 3 cut(s) 115, 210, 241
CviJI RGCY 9 cut(s) 30, 104, 156, 163, 171, 288, 325, 361, 384
CviKI_1 RGCY 9 cut(s) 30, 104, 156, 163, 171, 288, 325, 361, 384
CviQI GTAC 3 cut(s) 115, 210, 241
DdeI CTNAG 3 cut(s) 152, 187, 326
DpnI GATC 2 cut(s) 50, 281
DpnII GATC 2 cut(s) 48, 279
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco147I AGGCCT 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 25
FaiI YATR 3 cut(s) 231, 254, 414
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 20, 78
FokI GGATG 1 cut(s) 284
Fsp4HI GCNGC 2 cut(s) 20, 78
GlaI GCGC 1 cut(s) 148
GluI GCNGC 2 cut(s) 20, 78
HaeIII GGCC 1 cut(s) 30
HapII CCGG 2 cut(s) 125, 398
HhaI GCGC 1 cut(s) 149
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 286
HinfI GANTC 3 cut(s) 224, 343, 418
HpaII CCGG 2 cut(s) 125, 398
Hpy166II GTNNAC 4 cut(s) 97, 193, 249, 451
Hpy188I TCNGA 2 cut(s) 48, 423
Hpy188III TCNNGA 1 cut(s) 347
Hpy8I GTNNAC 4 cut(s) 97, 193, 249, 451
Hpy99I CGWCG 3 cut(s) 70, 86, 356
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 94, 340
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 294
HpyF3I CTNAG 3 cut(s) 152, 187, 326
HpySE526I ACGT 1 cut(s) 84
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 2 cut(s) 48, 279
LmnI GCTCC 2 cut(s) 176, 389
LpnPI CCDG 7 cut(s) 12, 39, 138, 149, 162, 411, 439
Lsp1109I GCAGC 1 cut(s) 6
LweI GCATC 2 cut(s) 306, 387
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 2 cut(s) 340, 347
MalI GATC 2 cut(s) 50, 281
MboI GATC 2 cut(s) 48, 279
MboII GAAGA 1 cut(s) 31
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 4 cut(s) 33, 365, 406, 472
MlyI GAGTC 3 cut(s) 218, 337, 427
MmeI TCCRAC 1 cut(s) 446
MnlI CCTC 2 cut(s) 103, 116
MseI TTAA 3 cut(s) 306, 468, 490
MspI CCGG 2 cut(s) 125, 398
MspR9I CCNGG 2 cut(s) 27, 125
Mva1269I GAATGC 1 cut(s) 132
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 153, 294
NciI CCSGG 1 cut(s) 125
NdeII GATC 2 cut(s) 48, 279
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 340, 347
PaeR7I CTCGAG 1 cut(s) 107
PceI AGGCCT 1 cut(s) 30
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 105, 114
PctI GAATGC 1 cut(s) 132
PkrI GCNGC 2 cut(s) 21, 79
PleI GAGTC 3 cut(s) 218, 337, 426
PpsI GAGTC 3 cut(s) 218, 337, 426
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 385
PspXI VCTCGAGB 1 cut(s) 107
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 3 cut(s) 116, 211, 242
RsaNI GTAC 3 cut(s) 115, 210, 241
SaqAI TTAA 3 cut(s) 306, 468, 490
SatI GCNGC 2 cut(s) 20, 78
Sau3AI GATC 2 cut(s) 48, 279
SchI GAGTC 3 cut(s) 218, 337, 427
ScrFI CCNGG 2 cut(s) 27, 125
SduI GDGCHC 1 cut(s) 315
SetI ASST 9 cut(s) 87, 158, 165, 173, 181, 290, 312, 363, 428
SfaNI GCATC 2 cut(s) 306, 387
Sfr274I CTCGAG 1 cut(s) 107
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 3 cut(s) 107, 283, 289
SmoI CTYRAG 3 cut(s) 107, 283, 289
Sse9I AATT 4 cut(s) 33, 365, 406, 472
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 4 cut(s) 78, 387, 435, 483
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 25, 123
TaaI ACNGT 2 cut(s) 94, 340
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 108, 222, 441
TaqII GACCGA 2 cut(s) 287, 361
TasI AATT 4 cut(s) 33, 365, 406, 472
TatI WGTACW 2 cut(s) 209, 240
TauI GCSGC 1 cut(s) 80
Tru1I TTAA 3 cut(s) 306, 468, 490
Tru9I TTAA 3 cut(s) 306, 468, 490
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 2 cut(s) 340, 347
TseI GCWGC 1 cut(s) 19
Tsp45I GTSAC 2 cut(s) 340, 347
TspRI CASTG 1 cut(s) 156
XapI RAATTY 2 cut(s) 365, 472
XhoI CTCGAG 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.