RchiOBHm_Chr6g0294201

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003757
Physical Location & Seq
Reverse (-)
56616209 .. 56617206
998 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26403

Sequence Viewer

Length: 501 bp
ATGTCTCTCCCAGCCATTCTCGCCGTTCTCGTCCTCCTTTCACCGTTCTCCTTCGCCGTCGCCGACGACGATACCCGGTCGGCCTACGACGTCATCCAGGACTTCAACTTCCCGATGGGGATTCTCCCCAAGGGCGTCACCGGCTACGACCTCGACCGGAGCACCGGCAAGTGGGCCGCCTATTTCGACGGCGCGTGTAGCTTCGCGCTCGAAGGCTCGTACCAGCTCAAGTACAAGTCCACCATCACCGGCACTATCTCCGAGAACAGGCTCAAGGACCTGACCGGAGTCAGCGTCAAGGTCTTGTTCCTGTGGCTTAATATCGTTGAGGTGTCTCGGAGCGGCGACGACCTGGATTTCTCGGTGGGGATCGCTTCGGCGTCGTTTCCGATTGACAATTTCTACGAGTGTCCGCAGTGCGGGTGTGGATTGAACTGCAGCCCTGCGCCGCCTCAAGTAAGGAAGATTAAGATGAAAAAATCTCTGGTTTCTTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

166

Amino Acids

18.03

Weight (kDa)

5.05

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 134 5.4e-35 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccBSI CCGCTC 1 cut(s) 342
AccII CGCG 2 cut(s) 194, 206
AciI CCGC 5 cut(s) 177, 342, 413, 420, 449
AclWI GGATC 1 cut(s) 377
AcyI GRCGYC 3 cut(s) 90, 135, 380
AfaI GTAC 2 cut(s) 221, 233
AfiI CCNNNNNNNGG 3 cut(s) 171, 267, 419
AgsI TTSAA 2 cut(s) 106, 433
AjnI CCWGG 2 cut(s) 96, 351
AluBI AGCT 2 cut(s) 201, 226
AluI AGCT 2 cut(s) 201, 226
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 9, 339
AlwI GGATC 1 cut(s) 377
AoxI GGCC 2 cut(s) 81, 174
ApeKI GCWGC 1 cut(s) 438
AspLEI GCGC 3 cut(s) 194, 208, 448
AspS9I GGNCC 2 cut(s) 174, 277
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 3 cut(s) 33, 130, 238
AvaII GGWCC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 450
BccI CCATC 2 cut(s) 109, 251
BceAI ACGGC 3 cut(s) 8, 41, 205
BciT130I CCWGG 2 cut(s) 98, 353
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 9, 339
BfmI CTRYAG 1 cut(s) 436
BisI GCNGC 4 cut(s) 177, 343, 439, 449
BlsI GCNGC 4 cut(s) 178, 344, 440, 450
Bme1390I CCNGG 3 cut(s) 76, 98, 353
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 2 cut(s) 174, 277
BmrFI CCNGG 3 cut(s) 76, 98, 353
BoxI GACNNNNGTC 1 cut(s) 287
BpuEI CTTGAG 3 cut(s) 212, 257, 438
BpuMI CCSGG 1 cut(s) 76
BsaHI GRCGYC 3 cut(s) 90, 135, 380
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 2 cut(s) 156, 284
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 267, 419
Bse118I RCCGGY 3 cut(s) 140, 164, 248
BseBI CCWGG 2 cut(s) 98, 353
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 171, 267, 419
BseXI GCAGC 1 cut(s) 450
BseYI CCCAGC 1 cut(s) 10
Bsh1236I CGCG 2 cut(s) 194, 206
Bsh1285I CGRYCG 2 cut(s) 80, 157
BshFI GGCC 2 cut(s) 83, 176
BsiEI CGRYCG 2 cut(s) 80, 157
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 6 cut(s) 76, 141, 157, 165, 249, 285
BslI CCNNNNNNNGG 3 cut(s) 171, 267, 419
BsmAI GTCTC 2 cut(s) 9, 339
BsnI GGCC 2 cut(s) 83, 176
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 1 cut(s) 369
BspACI CCGC 5 cut(s) 177, 342, 413, 420, 449
BspANI GGCC 2 cut(s) 83, 176
BspFNI CGCG 2 cut(s) 194, 206
BspMAI CTGCAG 1 cut(s) 440
BspPI GGATC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 342
BsrFI RCCGGY 3 cut(s) 140, 164, 248
BssAI RCCGGY 3 cut(s) 140, 164, 248
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 1 cut(s) 369
BssNI GRCGYC 3 cut(s) 90, 135, 380
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 98, 353
Bst4CI ACNGT 1 cut(s) 45
BstACI GRCGYC 3 cut(s) 90, 135, 380
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 194, 206
BstHHI GCGC 3 cut(s) 194, 208, 448
BstKTI GATC 1 cut(s) 372
BstMAI GTCTC 2 cut(s) 9, 339
BstMBI GATC 1 cut(s) 369
BstMCI CGRYCG 2 cut(s) 80, 157
BstMWI GCNNNNNNNGC 3 cut(s) 20, 141, 198
BstNI CCWGG 2 cut(s) 98, 353
BstPAI GACNNNNGTC 1 cut(s) 287
BstSCI CCNGG 3 cut(s) 74, 96, 351
BstSFI CTRYAG 1 cut(s) 436
BstUI CGCG 2 cut(s) 194, 206
BstV1I GCAGC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 83, 176
BtsCI GGATG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 1 cut(s) 422
CfoI GCGC 3 cut(s) 194, 208, 448
Cfr10I RCCGGY 3 cut(s) 140, 164, 248
Cfr13I GGNCC 2 cut(s) 174, 277
CseI GACGC 3 cut(s) 124, 283, 369
Csp6I GTAC 2 cut(s) 220, 232
CviQI GTAC 2 cut(s) 220, 232
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
Eco130I CCWWGG 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 96, 351
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FauI CCCGC 1 cut(s) 413
Fnu4HI GCNGC 4 cut(s) 177, 343, 439, 449
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 4 cut(s) 177, 343, 439, 449
GlaI GCGC 3 cut(s) 193, 207, 447
GluI GCNGC 4 cut(s) 177, 343, 439, 449
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 2 cut(s) 83, 176
HapII CCGG 6 cut(s) 76, 141, 157, 165, 249, 285
HgaI GACGC 3 cut(s) 124, 283, 369
HhaI GCGC 3 cut(s) 194, 208, 448
Hin1I GRCGYC 3 cut(s) 90, 135, 380
Hin6I GCGC 3 cut(s) 192, 206, 446
HinP1I GCGC 3 cut(s) 192, 206, 446
HinfI GANTC 2 cut(s) 121, 288
HpaII CCGG 6 cut(s) 76, 141, 157, 165, 249, 285
HphI GGTGA 3 cut(s) 33, 130, 238
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 3 cut(s) 262, 339, 390
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 7 cut(s) 62, 68, 71, 92, 191, 350, 385
HpyAV CCTTC 2 cut(s) 61, 206
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 1 cut(s) 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 141, 198
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 3 cut(s) 90, 135, 380
HspAI GCGC 3 cut(s) 192, 206, 446
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 2 cut(s) 159, 339
Lsp1109I GCAGC 1 cut(s) 450
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 136
MalI GATC 1 cut(s) 371
MbiI CCGCTC 1 cut(s) 342
MboI GATC 1 cut(s) 369
MboII GAAGA 2 cut(s) 475, 483
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 1 cut(s) 397
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 4 cut(s) 44, 161, 322, 462
MseI TTAA 2 cut(s) 318, 468
MspI CCGG 6 cut(s) 76, 141, 157, 165, 249, 285
MspR9I CCNGG 3 cut(s) 76, 98, 353
MvaI CCWGG 2 cut(s) 98, 353
MvnI CGCG 2 cut(s) 194, 206
MwoI GCNNNNNNNGC 3 cut(s) 20, 141, 198
NciI CCSGG 1 cut(s) 76
NdeII GATC 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 136
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 60, 66
PfeI GAWTC 1 cut(s) 121
PfoI TCCNGGA 1 cut(s) 96
PkrI GCNGC 4 cut(s) 178, 344, 440, 450
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PpuMI RGGWCCY 1 cut(s) 277
PshAI GACNNNNGTC 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 96, 351
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 2 cut(s) 96, 351
PspPI GGNCC 2 cut(s) 174, 277
PspPPI RGGWCCY 1 cut(s) 277
PstI CTGCAG 1 cut(s) 440
RsaI GTAC 2 cut(s) 221, 233
RsaNI GTAC 2 cut(s) 220, 232
SaqAI TTAA 2 cut(s) 318, 468
SatI GCNGC 4 cut(s) 177, 343, 439, 449
Sau3AI GATC 1 cut(s) 369
Sau96I GGNCC 2 cut(s) 174, 277
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 3 cut(s) 76, 98, 353
SduI GDGCHC 1 cut(s) 164
SetI ASST 8 cut(s) 93, 153, 203, 228, 282, 303, 333, 354
SfcI CTRYAG 1 cut(s) 436
SinI GGWCC 1 cut(s) 277
SmlI CTYRAG 3 cut(s) 227, 272, 453
SmoI CTYRAG 3 cut(s) 227, 272, 453
Sse9I AATT 1 cut(s) 397
SsiI CCGC 5 cut(s) 177, 342, 413, 420, 449
StyD4I CCNGG 3 cut(s) 74, 96, 351
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 153, 186, 210
TasI AATT 1 cut(s) 397
TatI WGTACW 1 cut(s) 231
TauI GCSGC 3 cut(s) 179, 345, 451
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 2 cut(s) 318, 468
Tru9I TTAA 2 cut(s) 318, 468
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 1 cut(s) 438
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 488
TspRI CASTG 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 277
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.