pycom11g26940

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003757
Physical Location & Seq
Forward (+)
29387869 .. 29388381
513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g26940.1

Sequence Viewer

Length: 513 bp
ATGTCTCCAAAGGAAACACTGCTACCAAGCCTAATTCTCTTCTTTCTGATCTCCATCTCAATTCCGTCATCCTTCGCCGCCGATGAGCTGACGGTTTACGAAGCCCTCGAGGAGTACGACTTCCCGGTCGGCATTCTCCCCAAAGGCGTACTGAGCTACAAGCTGGACAACTCGACAGGTAAGTTCTCAGTCTACTTGAATAGCACTTGTACATTTACCATCGACTCGTATAAGTTGAAGTACAAGTCCACCATAAAGGGTGTGATAAAGACTGACAAGATCTCAAGCTTGAGTGGCATCCAAGTTAAGGTGCTCTTTCTGTGGCTGAGCATTACCACCGTGACTCGTGACGACGATGAGCTAGAATTCTCGGTCGGCATCGGCTCCGCAAACTTTCCGGTCAGTAATTTCGATGAGAGTCCGACCTGTGGTTGCGGTTTCGATTGTGTCAACGGGAAAAGAAAGATTAAGAATTTGGTGTCCGCACTTTCAAGTCAATCTTTAGCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

171

Amino Acids

18.63

Weight (kDa)

5.17

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 138 1.2e-35 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AbsI CCTCGAGG 1 cut(s) 107
AccI GTMKAC 1 cut(s) 192
AciI CCGC 4 cut(s) 78, 387, 435, 483
AcsI RAATTY 2 cut(s) 365, 472
AfaI GTAC 4 cut(s) 116, 150, 211, 242
AfiI CCNNNNNNNGG 2 cut(s) 307, 428
AgsI TTSAA 3 cut(s) 199, 238, 492
AluBI AGCT 6 cut(s) 88, 156, 163, 288, 361, 506
AluI AGCT 6 cut(s) 88, 156, 163, 288, 361, 506
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 107
ApoI RAATTY 2 cut(s) 365, 472
AsuC2I CCSGG 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 107
BauI CACGAG 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 315
BccI CCATC 2 cut(s) 62, 227
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 362
BglII AGATCT 1 cut(s) 279
BisI GCNGC 1 cut(s) 78
BlpI GCTNAGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 306, 387
Bpu1102I GCTNAGC 1 cut(s) 326
BpuEI CTTGAG 2 cut(s) 268, 310
BpuMI CCSGG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 53
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 428
Bse8I GATNNNNATC 1 cut(s) 53
BseGI GGATG 2 cut(s) 68, 297
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 2 cut(s) 307, 428
BseMII CTCAG 3 cut(s) 143, 201, 317
BseRI GAGGAG 1 cut(s) 125
Bsh1285I CGRYCG 2 cut(s) 129, 375
BsiEI CGRYCG 2 cut(s) 129, 375
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 125, 398
BslI CCNNNNNNNGG 2 cut(s) 307, 428
BsmAI GTCTC 1 cut(s) 9
BsmI GAATGC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 209
Bsp143I GATC 2 cut(s) 48, 279
Bsp1720I GCTNAGC 1 cut(s) 326
BspACI CCGC 4 cut(s) 78, 387, 435, 483
BspCNI CTCAG 3 cut(s) 144, 200, 318
BspLI GGNNCC 1 cut(s) 385
BsrGI TGTACA 1 cut(s) 209
BssMI GATC 2 cut(s) 48, 279
BssSI CACGAG 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 94, 340
Bst6I CTCTTC 1 cut(s) 44
BstAUI TGTACA 1 cut(s) 209
BstDEI CTNAG 3 cut(s) 152, 187, 326
BstF5I GGATG 2 cut(s) 68, 297
BstKTI GATC 2 cut(s) 51, 282
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 48, 279
BstMCI CGRYCG 2 cut(s) 129, 375
BstMWI GCNNNNNNNGC 2 cut(s) 153, 294
BstSCI CCNGG 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BtsCI GGATG 2 cut(s) 68, 297
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 17
Csp6I GTAC 4 cut(s) 115, 149, 210, 241
CviQI GTAC 4 cut(s) 115, 149, 210, 241
DdeI CTNAG 3 cut(s) 152, 187, 326
DpnI GATC 2 cut(s) 50, 281
DpnII GATC 2 cut(s) 48, 279
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 365
FaiI YATR 2 cut(s) 231, 254
FalI AAGNNNNNCTT 3 cut(s) 299, 331, 484
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 2 cut(s) 55, 284
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 362
GluI GCNGC 1 cut(s) 78
HapII CCGG 2 cut(s) 125, 398
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 286
HinfI GANTC 3 cut(s) 224, 343, 418
HpaII CCGG 2 cut(s) 125, 398
Hpy166II GTNNAC 4 cut(s) 97, 193, 249, 451
Hpy188I TCNGA 2 cut(s) 48, 423
Hpy188III TCNNGA 1 cut(s) 347
Hpy8I GTNNAC 4 cut(s) 97, 193, 249, 451
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 94, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 294
HpyF3I CTNAG 3 cut(s) 152, 187, 326
Kzo9I GATC 2 cut(s) 48, 279
LmnI GCTCC 1 cut(s) 389
LpnPI CCDG 5 cut(s) 138, 149, 162, 411, 439
LweI GCATC 2 cut(s) 306, 387
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 340, 347
MalI GATC 2 cut(s) 50, 281
MboI GATC 2 cut(s) 48, 279
MboII GAAGA 1 cut(s) 31
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 5 cut(s) 33, 60, 365, 406, 472
MlyI GAGTC 3 cut(s) 218, 337, 427
MmeI TCCRAC 1 cut(s) 446
MnlI CCTC 2 cut(s) 103, 116
MseI TTAA 2 cut(s) 306, 468
MspI CCGG 2 cut(s) 125, 398
MspR9I CCNGG 1 cut(s) 125
Mva1269I GAATGC 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 153, 294
NciI CCSGG 1 cut(s) 125
NdeII GATC 2 cut(s) 48, 279
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 340, 347
PaeR7I CTCGAG 1 cut(s) 107
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 114
PctI GAATGC 1 cut(s) 132
PkrI GCNGC 1 cut(s) 79
PleI GAGTC 3 cut(s) 218, 337, 426
PpsI GAGTC 3 cut(s) 218, 337, 426
PspN4I GGNNCC 1 cut(s) 385
PspXI VCTCGAGB 1 cut(s) 107
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 4 cut(s) 116, 150, 211, 242
RsaNI GTAC 4 cut(s) 115, 149, 210, 241
SaqAI TTAA 2 cut(s) 306, 468
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 2 cut(s) 48, 279
SchI GAGTC 3 cut(s) 218, 337, 427
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 315
SetI ASST 9 cut(s) 90, 158, 165, 181, 290, 312, 363, 428, 508
SfaNI GCATC 2 cut(s) 306, 387
Sfr274I CTCGAG 1 cut(s) 107
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 3 cut(s) 107, 283, 289
SmoI CTYRAG 3 cut(s) 107, 283, 289
Sse9I AATT 5 cut(s) 33, 60, 365, 406, 472
SsiI CCGC 4 cut(s) 78, 387, 435, 483
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 94, 340
TaqI TCGA 5 cut(s) 108, 173, 222, 411, 441
TaqII GACCGA 1 cut(s) 361
TasI AATT 5 cut(s) 33, 60, 365, 406, 472
TatI WGTACW 2 cut(s) 209, 240
TauI GCSGC 1 cut(s) 80
Tru1I TTAA 2 cut(s) 306, 468
Tru9I TTAA 2 cut(s) 306, 468
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 2 cut(s) 340, 347
Tsp45I GTSAC 2 cut(s) 340, 347
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 1 cut(s) 24
XapI RAATTY 2 cut(s) 365, 472
XhoI CTCGAG 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.