MD03G1290200.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003757
Physical Location & Seq
Reverse (-)
36801217 .. 36801764
548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1290200.v1.1.491

Sequence Viewer

Length: 504 bp
ATGTCTCTCGCAACAATTCTCGCCGTCTTCGTCCTCCTATCACCGTTCTACACGTCATCAACCGCCTCCGCCAACGGCGACGGCTCGCGGTCTGTGTACGATGCAATCGAAGAATTCAACTTTCCGATGGGTCTGCTCCCGAAGGGCGTCACCGGATACGAGCTCGACAATGGCAACGGCCGATTCCGCGCGTATTTGAACGGCTCCTGCAGCTTCTCGCTGGAAGGCTCGTACCAGCTCAAGTACAAGTCCACCATTACCGGCACAATCTCCCCGAACAAGCTCTCCTCGCTGACCGGGGTCAGCGTCAAGGTCCTGTTCCTGTGGCTCGACATCGTCGAGGTGACCCGGAGCGGCGACAACCTCGAGTTCTCGGTGGGGATCGCGTCGGCGGCGTTTCCGATCGACAATTTTTACGAGTGCCCGCAGTGCGGGTGCGGGTTGGATTGCGTCAATGGCGGACAAGTAAGGAAGATTAAAATGCCTCTTGTTTCTTCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

168

Amino Acids

17.9

Weight (kDa)

5.07

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 138 2.4e-35 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 354
AccII CGCG 4 cut(s) 88, 189, 191, 386
AclWI GGATC 1 cut(s) 389
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 3 cut(s) 98, 233, 245
AfiI CCNNNNNNNGG 1 cut(s) 431
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 2 cut(s) 118, 199
AjiI CACGTC 1 cut(s) 54
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 4 cut(s) 163, 213, 238, 283
AluI AGCT 4 cut(s) 163, 213, 238, 283
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 389
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 210
ApoI RAATTY 1 cut(s) 113
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 1 cut(s) 313
AsuC2I CCSGG 2 cut(s) 298, 349
AsuHPI GGTGA 3 cut(s) 33, 142, 355
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 1 cut(s) 313
BaeGI GKGCMC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 222
BccI CCATC 1 cut(s) 121
BceAI ACGGC 5 cut(s) 8, 91, 97, 193, 217
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciVI GTATCC 1 cut(s) 149
BcnI CCSGG 2 cut(s) 298, 349
BcoDI GTCTC 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 208
BfuI GTATCC 1 cut(s) 149
BisI GCNGC 3 cut(s) 211, 355, 393
BlsI GCNGC 3 cut(s) 212, 356, 394
Bme1390I CCNGG 2 cut(s) 298, 349
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 2 cut(s) 298, 349
BmsI GCATC 1 cut(s) 91
BoxI GACNNNNGTC 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 2 cut(s) 298, 349
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 297
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse118I RCCGGY 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 431
BseRI GAGGAG 1 cut(s) 277
BseSI GKGCMC 1 cut(s) 425
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 222
Bsh1236I CGCG 4 cut(s) 88, 189, 191, 386
Bsh1285I CGRYCG 2 cut(s) 181, 405
BshFI GGCC 1 cut(s) 180
BsiEI CGRYCG 2 cut(s) 181, 405
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 4 cut(s) 153, 261, 297, 349
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 180
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 165, 425
Bsp143I GATC 2 cut(s) 381, 402
BspANI GGCC 1 cut(s) 180
BspFNI CGCG 4 cut(s) 88, 189, 191, 386
BspLI GGNNCC 1 cut(s) 205
BspMAI CTGCAG 1 cut(s) 212
BspPI GGATC 1 cut(s) 389
BsrBI CCGCTC 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 2 cut(s) 381, 402
BssNI GRCGYC 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 2 cut(s) 86, 425
BstEII GGTNACC 1 cut(s) 343
BstFNI CGCG 4 cut(s) 88, 189, 191, 386
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 2 cut(s) 384, 405
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 381, 402
BstMCI CGRYCG 2 cut(s) 181, 405
BstMWI GCNNNNNNNGC 6 cut(s) 186, 210, 289, 392, 429, 456
BstPAI GACNNNNGTC 1 cut(s) 299
BstPI GGTNACC 1 cut(s) 343
BstSCI CCNGG 2 cut(s) 296, 347
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 425
BstUI CGCG 4 cut(s) 88, 189, 191, 386
BstV1I GCAGC 1 cut(s) 222
BstV2I GAAGAC 1 cut(s) 19
BstZI CGGCCG 1 cut(s) 178
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 1 cut(s) 180
BtrI CACGTC 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 1 cut(s) 434
Cac8I GCNNGC 2 cut(s) 86, 425
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 313
CseI GACGC 4 cut(s) 136, 295, 375, 439
Csp6I GTAC 3 cut(s) 97, 232, 244
CviJI RGCY 9 cut(s) 84, 163, 180, 204, 213, 228, 238, 283, 328
CviKI_1 RGCY 9 cut(s) 84, 163, 180, 204, 213, 228, 238, 283, 328
CviQI GTAC 3 cut(s) 97, 232, 244
DpnI GATC 2 cut(s) 383, 404
DpnII GATC 2 cut(s) 381, 402
EaeI YGGCCR 1 cut(s) 178
EagI CGGCCG 1 cut(s) 178
EciI GGCGGA 2 cut(s) 58, 474
Ecl136II GAGCTC 1 cut(s) 163
EclXI CGGCCG 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 313
Eco52I CGGCCG 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 163
Eco88I CYCGRG 1 cut(s) 365
Eco91I GGTNACC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 163
EcoO109I RGGNCCY 1 cut(s) 313
EcoO65I GGTNACC 1 cut(s) 343
EcoRI GAATTC 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 165
FauI CCCGC 3 cut(s) 425, 431, 432
Fnu4HI GCNGC 3 cut(s) 211, 355, 393
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 3 cut(s) 211, 355, 393
GlaI GCGC 1 cut(s) 190
GluI GCNGC 3 cut(s) 211, 355, 393
HaeIII GGCC 1 cut(s) 180
HapII CCGG 4 cut(s) 153, 261, 297, 349
HgaI GACGC 4 cut(s) 136, 295, 375, 439
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 147
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 1 cut(s) 183
HpaII CCGG 4 cut(s) 153, 261, 297, 349
HphI GGTGA 3 cut(s) 33, 142, 355
Hpy166II GTNNAC 2 cut(s) 97, 252
Hpy188I TCNGA 2 cut(s) 126, 402
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 2 cut(s) 97, 252
Hpy99I CGWCG 3 cut(s) 83, 341, 391
HpyAV CCTTC 2 cut(s) 136, 218
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 2 cut(s) 104, 210
HpyF10VI GCNNNNNNNGC 6 cut(s) 186, 210, 289, 392, 429, 456
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 1 cut(s) 147
HspAI GCGC 1 cut(s) 189
Kzo9I GATC 2 cut(s) 381, 402
LmnI GCTCC 3 cut(s) 141, 209, 351
LpnPI CCDG 9 cut(s) 166, 206, 220, 248, 274, 310, 329, 335, 362
Lsp1109I GCAGC 1 cut(s) 222
LweI GCATC 1 cut(s) 91
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 148, 343
MalI GATC 2 cut(s) 383, 404
MbiI CCGCTC 1 cut(s) 354
MboI GATC 2 cut(s) 381, 402
MboII GAAGA 4 cut(s) 19, 122, 484, 486
MhlI GDGCHC 2 cut(s) 165, 425
MluCI AATT 3 cut(s) 15, 113, 409
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 6 cut(s) 44, 76, 298, 334, 374, 495
MseI TTAA 1 cut(s) 477
MspI CCGG 4 cut(s) 153, 261, 297, 349
MspR9I CCNGG 2 cut(s) 298, 349
MvnI CGCG 4 cut(s) 88, 189, 191, 386
MwoI GCNNNNNNNGC 6 cut(s) 186, 210, 289, 392, 429, 456
NciI CCSGG 2 cut(s) 298, 349
NdeII GATC 2 cut(s) 381, 402
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 148, 343
PaeR7I CTCGAG 1 cut(s) 365
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 105, 336
PfeI GAWTC 1 cut(s) 183
PflFI GACNNNGTC 1 cut(s) 335
PkrI GCNGC 3 cut(s) 212, 356, 394
Ple19I CGATCG 1 cut(s) 405
PpuMI RGGWCCY 1 cut(s) 313
PshAI GACNNNNGTC 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 165
Psp5II RGGWCCY 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 313
PspPPI RGGWCCY 1 cut(s) 313
PspXI VCTCGAGB 1 cut(s) 365
PstI CTGCAG 1 cut(s) 212
PsyI GACNNNGTC 1 cut(s) 335
PvuI CGATCG 1 cut(s) 405
RsaI GTAC 3 cut(s) 98, 233, 245
RsaNI GTAC 3 cut(s) 97, 232, 244
SacI GAGCTC 1 cut(s) 165
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 3 cut(s) 211, 355, 393
Sau3AI GATC 2 cut(s) 381, 402
Sau96I GGNCC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 298, 349
SduI GDGCHC 2 cut(s) 165, 425
SetI ASST 8 cut(s) 56, 165, 215, 240, 285, 315, 345, 366
SfaNI GCATC 1 cut(s) 91
SfcI CTRYAG 1 cut(s) 208
Sfr274I CTCGAG 1 cut(s) 365
SinI GGWCC 1 cut(s) 313
SlaI CTCGAG 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 239, 365
SmoI CTYRAG 2 cut(s) 239, 365
Sse9I AATT 3 cut(s) 15, 113, 409
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 2 cut(s) 296, 347
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 1 cut(s) 56
TaqI TCGA 7 cut(s) 108, 165, 330, 339, 366, 405, 497
TasI AATT 3 cut(s) 15, 113, 409
TatI WGTACW 1 cut(s) 243
TauI GCSGC 2 cut(s) 357, 395
TfiI GAWTC 1 cut(s) 183
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 1 cut(s) 434
TseFI GTSAC 2 cut(s) 148, 343
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 2 cut(s) 148, 343
TspRI CASTG 1 cut(s) 434
Tth111I GACNNNGTC 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 1 cut(s) 113
XhoI CTCGAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.