Rw6G030910

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003757
Physical Location & Seq
Reverse (-)
54998217 .. 55000053
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G030910.1

Sequence Viewer

Length: 501 bp
ATGGCAACACTAGGCCAAATACTCTTCTTTCTGGTCTCCTTCTCAATCTCAACCCCATCTGCTTCTGTCAAGGACAAGCTGACCGCTTACGAAGCTCTCGAAGAGTATGACTTCCCAGTAGGCATTCTTCCCAAAGGCGTACTAAGCTACGAGCTAGACAATTCCACAGGTAAATTCTCAGTCTATTTGAATGGCTCTTGTACCTTTTCAATAGATTCATATAAGCTCAAGTACAAGTCCACCATAACGGGTGTGATAACCAAAGACAAGATCTCGAGCTTGAGTGGTATCACGGTCAAGGTCTTGTTCCTGTGGCTCAATATCGTTGAGGTGTCTCGGAGCGGCGACGACCTGGATTTCTCGGTGGGGATCGCTTCGGCGTCGTTTCCGATTGACAATTTCTACGAGTGTCCGCAGTGCGGGTGTGGATTGAACTGCAGCCCTGCGCCGCCTCAAGTAAGGAAGATTAAGATGAAAAAATCTCTGGTTTCTTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

166

Amino Acids

18.05

Weight (kDa)

7.53

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 29 - 134 8.3e-37 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 342
AciI CCGC 5 cut(s) 84, 342, 413, 420, 449
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 1 cut(s) 173
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 3 cut(s) 141, 202, 233
AfiI CCNNNNNNNGG 1 cut(s) 419
AgsI TTSAA 3 cut(s) 190, 210, 433
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 6 cut(s) 79, 95, 147, 154, 226, 279
AluI AGCT 6 cut(s) 79, 95, 147, 154, 226, 279
Alw26I GTCTC 2 cut(s) 40, 339
AlwI GGATC 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 274
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 1 cut(s) 438
ApoI RAATTY 1 cut(s) 173
AspLEI GCGC 1 cut(s) 448
AvaI CYCGRG 1 cut(s) 274
BarI GAAGNNNNNNTAC 2 cut(s) 111, 143
BbvI GCAGC 1 cut(s) 450
BccI CCATC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 40, 339
BfaI CTAG 2 cut(s) 11, 155
BfmI CTRYAG 1 cut(s) 436
BglII AGATCT 1 cut(s) 270
BisI GCNGC 3 cut(s) 343, 439, 449
BlsI GCNGC 3 cut(s) 344, 440, 450
Bme1390I CCNGG 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 353
BmrI ACTGGG 1 cut(s) 110
BmuI ACTGGG 1 cut(s) 110
BpuEI CTTGAG 3 cut(s) 212, 301, 438
BsaHI GRCGYC 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 353
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 116
BseXI GCAGC 1 cut(s) 450
BshFI GGCC 1 cut(s) 15
BsiHKCI CYCGRG 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 419
BsmAI GTCTC 2 cut(s) 40, 339
BsmI GAATGC 1 cut(s) 123
BsnI GGCC 1 cut(s) 15
Bso31I GGTCTC 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 274
Bsp143I GATC 2 cut(s) 270, 369
BspACI CCGC 5 cut(s) 84, 342, 413, 420, 449
BspANI GGCC 1 cut(s) 15
BspCNI CTCAG 1 cut(s) 191
BspMAI CTGCAG 1 cut(s) 440
BspPI GGATC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 342
BsrI ACTGG 1 cut(s) 116
BssMI GATC 2 cut(s) 270, 369
BssNI GRCGYC 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 2 cut(s) 29, 96
BstACI GRCGYC 1 cut(s) 380
BstDEI CTNAG 2 cut(s) 143, 178
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 2 cut(s) 273, 372
BstMAI GTCTC 2 cut(s) 40, 339
BstMBI GATC 2 cut(s) 270, 369
BstMWI GCNNNNNNNGC 2 cut(s) 92, 144
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 351
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 450
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 1 cut(s) 15
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 448
CseI GACGC 1 cut(s) 369
Csp6I GTAC 3 cut(s) 140, 201, 232
CviQI GTAC 3 cut(s) 140, 201, 232
DdeI CTNAG 2 cut(s) 143, 178
DpnI GATC 2 cut(s) 272, 371
DpnII GATC 2 cut(s) 270, 369
Eam1104I CTCTTC 2 cut(s) 29, 96
EarI CTCTTC 2 cut(s) 29, 96
Eco31I GGTCTC 1 cut(s) 40
Eco88I CYCGRG 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 351
FaiI YATR 4 cut(s) 108, 220, 222, 245
FauI CCCGC 1 cut(s) 413
Fnu4HI GCNGC 3 cut(s) 343, 439, 449
Fsp4HI GCNGC 3 cut(s) 343, 439, 449
FspBI CTAG 2 cut(s) 11, 155
GlaI GCGC 1 cut(s) 447
GluI GCNGC 3 cut(s) 343, 439, 449
HaeIII GGCC 1 cut(s) 15
HgaI GACGC 1 cut(s) 369
HhaI GCGC 1 cut(s) 448
Hin1I GRCGYC 1 cut(s) 380
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 2 cut(s) 339, 390
Hpy188III TCNNGA 2 cut(s) 98, 274
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 2 cut(s) 350, 385
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4V TGCA 1 cut(s) 438
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 144
HpyF3I CTNAG 2 cut(s) 143, 178
Hsp92I GRCGYC 1 cut(s) 380
HspAI GCGC 1 cut(s) 446
Kzo9I GATC 2 cut(s) 270, 369
LmnI GCTCC 1 cut(s) 339
LpnPI CCDG 8 cut(s) 17, 129, 153, 323, 338, 365, 456, 470
Lsp1109I GCAGC 1 cut(s) 450
MaeI CTAG 2 cut(s) 11, 155
MalI GATC 2 cut(s) 272, 371
MbiI CCGCTC 1 cut(s) 342
MboI GATC 2 cut(s) 270, 369
MboII GAAGA 5 cut(s) 16, 113, 119, 475, 483
MflI RGATCY 1 cut(s) 270
MluCI AATT 3 cut(s) 160, 173, 397
MnlI CCTC 2 cut(s) 322, 462
MseI TTAA 1 cut(s) 468
MspR9I CCNGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 123
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 92, 144
NdeII GATC 2 cut(s) 270, 369
PaeR7I CTCGAG 1 cut(s) 274
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 3 cut(s) 344, 440, 450
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PstI CTGCAG 1 cut(s) 440
PsuI RGATCY 1 cut(s) 270
RsaI GTAC 3 cut(s) 141, 202, 233
RsaNI GTAC 3 cut(s) 140, 201, 232
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 3 cut(s) 343, 439, 449
Sau3AI GATC 2 cut(s) 270, 369
ScrFI CCNGG 1 cut(s) 353
SfcI CTRYAG 1 cut(s) 436
Sfr274I CTCGAG 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 274
SmlI CTYRAG 4 cut(s) 227, 274, 280, 453
SmoI CTYRAG 4 cut(s) 227, 274, 280, 453
Sse9I AATT 3 cut(s) 160, 173, 397
SsiI CCGC 5 cut(s) 84, 342, 413, 420, 449
SspMI CTAG 2 cut(s) 11, 155
StyD4I CCNGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 2 cut(s) 99, 275
TasI AATT 3 cut(s) 160, 173, 397
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 345, 451
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 1 cut(s) 422
TseI GCWGC 1 cut(s) 438
TspDTI ATGAA 2 cut(s) 207, 488
TspRI CASTG 1 cut(s) 422
XapI RAATTY 1 cut(s) 173
XhoI CTCGAG 1 cut(s) 274
XspI CTAG 2 cut(s) 11, 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.