MD11G1308300.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003757
Physical Location & Seq
Forward (+)
42204179 .. 42204685
507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1308300.v1.1.491

Sequence Viewer

Length: 507 bp
ATGTCACGCACAGCAATTATTGCCGTCATCGTCCTCCTATCACCGTTCTCCACGTCACCAACCCTCTCCACCGCTGCCAACGGTGGTGAATCGCGGTCTGTGTACGATGCAATCGAAGACTTTAACTTTCCGATGGGTCTGCTCCCCAAGGGCGTCACGGGATACGATCTCGACGGCAACGGAATATTCCGCGCGTATCTGAATGGCTCCTGTAGCTTCTCGCTGGAAGGGTCGTACCAGCTCAAGTACAAGTCCACTATTACCGGCACGATCTCCCAGAACAAGCTCTCCTCGCTGACCGGGGTGAGCGTCAAGGTCCTGTTCCTGTGGCTCAACATCGTCGAGGTCACCCGGAGCGGCGACAACCTTGACTTCTCGGTGGGGATCGCGTCGGCGGCGTTTCCGATCGACAATTTCTATGAGTGCCCGCAGTGCGGCTGCGGGTTGGATTGCGTCAATGGCGGACAAGTAAGGAAGATTAAAATGCCTCTTGTTTCTTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

169

Amino Acids

17.93

Weight (kDa)

5.32

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 34 - 139 1.7e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 357
AccII CGCG 4 cut(s) 94, 192, 194, 389
AciI CCGC 9 cut(s) 72, 94, 190, 357, 395, 428, 435, 441, 462
AclWI GGATC 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 104, 236, 248
AfiI CCNNNNNNNGG 1 cut(s) 434
AjiI CACGTC 1 cut(s) 54
AloI GAACNNNNNNTCC 2 cut(s) 272, 304
AluBI AGCT 3 cut(s) 216, 241, 286
AluI AGCT 3 cut(s) 216, 241, 286
AlwI GGATC 1 cut(s) 392
ApeKI GCWGC 2 cut(s) 74, 438
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 2 cut(s) 301, 352
AsuHPI GGTGA 5 cut(s) 33, 48, 98, 316, 340
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 61, 425
BccI CCATC 1 cut(s) 127
BceAI ACGGC 2 cut(s) 8, 190
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciVI GTATCC 1 cut(s) 155
BcnI CCSGG 2 cut(s) 301, 352
BfmI CTRYAG 1 cut(s) 211
BfuI GTATCC 1 cut(s) 155
BisI GCNGC 5 cut(s) 75, 358, 396, 436, 439
BlsI GCNGC 5 cut(s) 76, 359, 397, 437, 440
Bme1390I CCNGG 2 cut(s) 301, 352
Bme18I GGWCC 1 cut(s) 316
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 2 cut(s) 301, 352
BmsI GCATC 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 2 cut(s) 301, 352
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 147, 300
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse118I RCCGGY 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 147, 300
BseLI CCNNNNNNNGG 1 cut(s) 434
BseRI GAGGAG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 2 cut(s) 61, 425
Bsh1236I CGCG 4 cut(s) 94, 192, 194, 389
Bsh1285I CGRYCG 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 408
BsiSI CCGG 3 cut(s) 264, 300, 352
BslI CCNNNNNNNGG 1 cut(s) 434
Bsp1286I GDGCHC 1 cut(s) 428
Bsp143I GATC 4 cut(s) 166, 270, 384, 405
BspACI CCGC 9 cut(s) 72, 94, 190, 357, 395, 428, 435, 441, 462
BspFNI CGCG 4 cut(s) 94, 192, 194, 389
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 1 cut(s) 392
BsrBI CCGCTC 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 147, 300
BssMI GATC 4 cut(s) 166, 270, 384, 405
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 45, 83
BstACI GRCGYC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 428
BstEII GGTNACC 1 cut(s) 346
BstFNI CGCG 4 cut(s) 94, 192, 194, 389
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 4 cut(s) 169, 273, 387, 408
BstMBI GATC 4 cut(s) 166, 270, 384, 405
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 6 cut(s) 20, 213, 292, 395, 432, 459
BstPI GGTNACC 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 299, 350
BstSFI CTRYAG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 4 cut(s) 94, 192, 194, 389
BstV1I GCAGC 2 cut(s) 61, 425
BstV2I GAAGAC 1 cut(s) 123
BsuI GTATCC 1 cut(s) 155
BtrI CACGTC 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 1 cut(s) 437
Cac8I GCNNGC 1 cut(s) 428
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 4 cut(s) 142, 298, 378, 442
Csp6I GTAC 3 cut(s) 103, 235, 247
CviJI RGCY 6 cut(s) 207, 216, 241, 286, 331, 438
CviKI_1 RGCY 6 cut(s) 207, 216, 241, 286, 331, 438
CviQI GTAC 3 cut(s) 103, 235, 247
DpnI GATC 4 cut(s) 168, 272, 386, 407
DpnII GATC 4 cut(s) 166, 270, 384, 405
EciI GGCGGA 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 147
Eco47I GGWCC 1 cut(s) 316
Eco91I GGTNACC 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 316
EcoO65I GGTNACC 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaiI YATR 1 cut(s) 420
FauI CCCGC 2 cut(s) 434, 435
Fnu4HI GCNGC 5 cut(s) 75, 358, 396, 436, 439
Fsp4HI GCNGC 5 cut(s) 75, 358, 396, 436, 439
GlaI GCGC 1 cut(s) 193
GluI GCNGC 5 cut(s) 75, 358, 396, 436, 439
HapII CCGG 3 cut(s) 264, 300, 352
HgaI GACGC 4 cut(s) 142, 298, 378, 442
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 1 cut(s) 89
HpaII CCGG 3 cut(s) 264, 300, 352
HphI GGTGA 5 cut(s) 33, 48, 98, 316, 340
Hpy166II GTNNAC 2 cut(s) 103, 255
Hpy188I TCNGA 3 cut(s) 132, 201, 405
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 2 cut(s) 103, 255
Hpy99I CGWCG 3 cut(s) 176, 344, 394
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 2 cut(s) 45, 83
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 1 cut(s) 110
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 213, 292, 395, 432, 459
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 1 cut(s) 192
Kzo9I GATC 4 cut(s) 166, 270, 384, 405
LmnI GCTCC 3 cut(s) 147, 212, 354
LpnPI CCDG 9 cut(s) 209, 223, 251, 277, 290, 313, 332, 338, 365
Lsp1109I GCAGC 2 cut(s) 61, 425
LweI GCATC 1 cut(s) 97
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 4 cut(s) 3, 54, 154, 346
MalI GATC 4 cut(s) 168, 272, 386, 407
MbiI CCGCTC 1 cut(s) 357
MboI GATC 4 cut(s) 166, 270, 384, 405
MboII GAAGA 3 cut(s) 128, 487, 489
MhlI GDGCHC 1 cut(s) 428
MluCI AATT 2 cut(s) 15, 412
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 5 cut(s) 44, 74, 301, 337, 498
MseI TTAA 2 cut(s) 123, 480
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 3 cut(s) 264, 300, 352
MspR9I CCNGG 2 cut(s) 301, 352
MvnI CGCG 4 cut(s) 94, 192, 194, 389
MwoI GCNNNNNNNGC 6 cut(s) 20, 213, 292, 395, 432, 459
NciI CCSGG 2 cut(s) 301, 352
NdeII GATC 4 cut(s) 166, 270, 384, 405
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 4 cut(s) 3, 54, 154, 346
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 5 cut(s) 76, 359, 397, 437, 440
Ple19I CGATCG 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 316
Psp5II RGGWCCY 1 cut(s) 316
PspEI GGTNACC 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 316
PvuI CGATCG 1 cut(s) 408
RsaI GTAC 3 cut(s) 104, 236, 248
RsaNI GTAC 3 cut(s) 103, 235, 247
SaqAI TTAA 2 cut(s) 123, 480
SatI GCNGC 5 cut(s) 75, 358, 396, 436, 439
Sau3AI GATC 4 cut(s) 166, 270, 384, 405
Sau96I GGNCC 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 301, 352
SduI GDGCHC 1 cut(s) 428
SetI ASST 7 cut(s) 56, 218, 243, 288, 318, 348, 369
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 211
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 2 cut(s) 15, 412
SsiI CCGC 9 cut(s) 72, 94, 190, 357, 395, 428, 435, 441, 462
SspI AATATT 1 cut(s) 186
StyD4I CCNGG 2 cut(s) 299, 350
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 2 cut(s) 45, 83
TaiI ACGT 1 cut(s) 56
TaqI TCGA 4 cut(s) 114, 171, 342, 408
TasI AATT 2 cut(s) 15, 412
TatI WGTACW 1 cut(s) 246
TauI GCSGC 3 cut(s) 360, 398, 438
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 123, 480
Tru9I TTAA 2 cut(s) 123, 480
TscAI CASTG 1 cut(s) 437
TseFI GTSAC 4 cut(s) 3, 54, 154, 346
TseI GCWGC 2 cut(s) 74, 438
Tsp45I GTSAC 4 cut(s) 3, 54, 154, 346
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.