MD03G1290300.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003757
Physical Location & Seq
Reverse (-)
36808549 .. 36809374
826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1290300.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTCTCCAAAGGCAACACTGCTACCAAGCCTAATTCTCTTCTTTCTGCTCTCTTTCTTGATCCCCTCGTCCTTCGCTGCCGACGAGCTGACGGTTTACGAAGCCCTCGAGGAGTACGACTTCCCGGTGGGCATACTCCCCAAAGACGTAGTGAGCTACGAGCTGGACACCTCCACAGGTAAGTTCTCAGTCTACTTGAACAGCACTTGTAGATTTACCATCGACTCGTATAAGCTGAAGTACAAGTCCACCATAACGGGTATTGTAACCACTGGCAAGATCTCTAGCTTGAGTGGCATCGAAGTTAGGGTGCTCTTTCTGTGGCTGAGCATTGTCTCAGTGACTCGTGACGACGATGAGCTAGAATTATCGGTCGGCATCGCCTCCGCAAACTTTCCGGTGAGTAATTTCGATGAAAGTCCGACCTGTGGTTGTGGTTTCGACTGTGTGAATGGGAAAAGAATGATTAAGAATTTGGTGTCTGCTCTTTTAGGTCAGTCTTTAGCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

171

Amino Acids

18.6

Weight (kDa)

4.6

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 138 2.8e-35 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 107
AccI GTMKAC 1 cut(s) 192
AciI CCGC 1 cut(s) 387
AclWI GGATC 1 cut(s) 55
AcsI RAATTY 1 cut(s) 472
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 2 cut(s) 116, 242
AfiI CCNNNNNNNGG 1 cut(s) 428
AgsI TTSAA 1 cut(s) 199
AluBI AGCT 7 cut(s) 88, 156, 163, 235, 288, 361, 506
AluI AGCT 7 cut(s) 88, 156, 163, 235, 288, 361, 506
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 9, 340
AlwI GGATC 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 107
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 1 cut(s) 472
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 412
AvaI CYCGRG 1 cut(s) 107
BauI CACGAG 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 227
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 9, 340
BfaI CTAG 2 cut(s) 285, 362
BglII AGATCT 1 cut(s) 279
BisI GCNGC 1 cut(s) 78
BlpI GCTNAGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 107
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 306, 387
Bpu1102I GCTNAGC 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 310
BpuMI CCSGG 1 cut(s) 125
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 3 cut(s) 201, 317, 351
BseNI ACTGG 1 cut(s) 277
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 375
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 125, 398
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 2 cut(s) 9, 340
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 2 cut(s) 60, 279
Bsp1720I GCTNAGC 1 cut(s) 326
BspACI CCGC 1 cut(s) 387
BspCNI CTCAG 3 cut(s) 200, 318, 350
BspPI GGATC 1 cut(s) 55
BsrI ACTGG 1 cut(s) 277
BssMI GATC 2 cut(s) 60, 279
BssSI CACGAG 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 94, 446
Bst6I CTCTTC 1 cut(s) 44
BstDEI CTNAG 3 cut(s) 187, 326, 337
BstKTI GATC 2 cut(s) 63, 282
BstMAI GTCTC 2 cut(s) 9, 340
BstMBI GATC 2 cut(s) 60, 279
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 1 cut(s) 294
BstSCI CCNGG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 3 cut(s) 17, 270, 345
Csp6I GTAC 2 cut(s) 115, 241
CviQI GTAC 2 cut(s) 115, 241
DdeI CTNAG 3 cut(s) 187, 326, 337
DpnI GATC 2 cut(s) 62, 281
DpnII GATC 2 cut(s) 60, 279
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 107
FaiI YATR 3 cut(s) 134, 231, 254
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 2 cut(s) 285, 362
GluI GCNGC 1 cut(s) 78
HapII CCGG 2 cut(s) 125, 398
HinfI GANTC 2 cut(s) 224, 343
HpaII CCGG 2 cut(s) 125, 398
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 3 cut(s) 97, 193, 249
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 2 cut(s) 58, 347
Hpy8I GTNNAC 3 cut(s) 97, 193, 249
Hpy99I CGWCG 2 cut(s) 86, 356
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 94, 446
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 294
HpyF3I CTNAG 3 cut(s) 187, 326, 337
HpySE526I ACGT 1 cut(s) 147
Kzo9I GATC 2 cut(s) 60, 279
LpnPI CCDG 6 cut(s) 138, 149, 162, 258, 411, 439
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 2 cut(s) 306, 387
MaeI CTAG 2 cut(s) 285, 362
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 265, 340, 347
MalI GATC 2 cut(s) 62, 281
MboI GATC 2 cut(s) 60, 279
MboII GAAGA 1 cut(s) 31
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 4 cut(s) 33, 365, 406, 472
MlyI GAGTC 2 cut(s) 218, 337
MmeI TCCRAC 1 cut(s) 446
MnlI CCTC 5 cut(s) 76, 103, 116, 181, 394
MseI TTAA 1 cut(s) 468
MspI CCGG 2 cut(s) 125, 398
MspR9I CCNGG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 294
NciI CCSGG 1 cut(s) 125
NdeII GATC 2 cut(s) 60, 279
NmuCI GTSAC 2 cut(s) 340, 347
PaeR7I CTCGAG 1 cut(s) 107
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 105, 114
PkrI GCNGC 1 cut(s) 79
PleI GAGTC 2 cut(s) 218, 337
PpsI GAGTC 2 cut(s) 218, 337
PspXI VCTCGAGB 1 cut(s) 107
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 2 cut(s) 116, 242
RsaNI GTAC 2 cut(s) 115, 241
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 2 cut(s) 60, 279
SchI GAGTC 2 cut(s) 218, 337
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 315
SfaNI GCATC 2 cut(s) 306, 387
Sfr274I CTCGAG 1 cut(s) 107
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 2 cut(s) 107, 289
SmoI CTYRAG 2 cut(s) 107, 289
Sse9I AATT 4 cut(s) 33, 365, 406, 472
SsiI CCGC 1 cut(s) 387
SspMI CTAG 2 cut(s) 285, 362
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 94, 446
TaiI ACGT 1 cut(s) 150
TaqI TCGA 5 cut(s) 108, 222, 300, 411, 441
TaqII GACCGA 1 cut(s) 361
TasI AATT 4 cut(s) 33, 365, 406, 472
TatI WGTACW 1 cut(s) 240
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 3 cut(s) 24, 277, 345
TseFI GTSAC 2 cut(s) 340, 347
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 2 cut(s) 340, 347
TspDTI ATGAA 1 cut(s) 429
TspRI CASTG 3 cut(s) 24, 277, 345
XapI RAATTY 1 cut(s) 472
XhoI CTCGAG 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 2 cut(s) 285, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.