MD03G1290000.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003757
Physical Location & Seq
Reverse (-)
36798798 .. 36799307
510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1290000.v1.1.491

Sequence Viewer

Length: 510 bp
ATGTCTCTCGCAACAACTCTCGCCGTCCTCGTCCTCCTATCACCGTTGTCCACGTCATCAACCGCCTCCGCCAACGGCGATGGCGACGGCTCGCTGTCTGTGTACGATGCAATCCAAGAATTCAACTTTCCGATGGGTCTGCTCCCGAAGGGCGTCACCGGATACGAGCTCGACAATGGCAACGGCCGATTCCTCGCGTATTTGAACGGCTCCTGCAGCTTCTCGCTGGAAGGCTCGTACCAGCTCAAGTACAAGTCCACCATTACCGGCACAATCTCCCCGAACAAGCTCTCCTCGCTGACCGGGGTCAGCGTCAAGGTCCTGTTCCTGTGGCTCGACATCGTCGAGGTCACCCGGAGAGGTGACAACCTCGAGTTCTCGGTGGGGATAGCGTCGGCGGCGTTTCCAATCGACAATTTTTACGAGTGCCCGCAGTGCGGGTGCGGGTTGGATTGCTTCAATGGCGGACAAGTAAGGAAGATTAAAATGCCTCTTGTTTCTTCGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

170

Amino Acids

17.93

Weight (kDa)

4.82

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 34 - 140 8.9e-35 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 197
AciI CCGC 7 cut(s) 63, 69, 398, 431, 438, 444, 465
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 1 cut(s) 119
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 104, 239, 251
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 3 cut(s) 124, 205, 460
AjiI CACGTC 1 cut(s) 54
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
AluBI AGCT 4 cut(s) 169, 219, 244, 289
AluI AGCT 4 cut(s) 169, 219, 244, 289
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 9
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 216
ApoI RAATTY 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 319
AsuC2I CCSGG 2 cut(s) 304, 355
AsuHPI GGTGA 4 cut(s) 33, 148, 343, 374
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 1 cut(s) 431
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 228
BccI CCATC 2 cut(s) 74, 127
BceAI ACGGC 5 cut(s) 8, 91, 103, 199, 223
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciVI GTATCC 1 cut(s) 155
BcnI CCSGG 2 cut(s) 304, 355
BcoDI GTCTC 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 214
BfuI GTATCC 1 cut(s) 155
BisI GCNGC 2 cut(s) 217, 399
BlsI GCNGC 2 cut(s) 218, 400
Bme1390I CCNGG 2 cut(s) 304, 355
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 371
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 304, 355
BmsI GCATC 1 cut(s) 97
BoxI GACNNNNGTC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 2 cut(s) 304, 355
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse118I RCCGGY 1 cut(s) 266
BseDI CCNNGG 1 cut(s) 303
BseLI CCNNNNNNNGG 1 cut(s) 437
BseRI GAGGAG 1 cut(s) 283
BseSI GKGCMC 1 cut(s) 431
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 1 cut(s) 228
Bsh1236I CGCG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 4 cut(s) 159, 267, 303, 355
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 171, 431
BspACI CCGC 7 cut(s) 63, 69, 398, 431, 438, 444, 465
BspANI GGCC 1 cut(s) 186
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 266
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 303
BssNI GRCGYC 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 2 cut(s) 92, 431
BstEII GGTNACC 1 cut(s) 349
BstFNI CGCG 1 cut(s) 197
BstMAI GTCTC 1 cut(s) 9
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 5 cut(s) 216, 295, 398, 435, 462
BstPAI GACNNNNGTC 1 cut(s) 305
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 2 cut(s) 302, 353
BstSFI CTRYAG 1 cut(s) 214
BstSLI GKGCMC 1 cut(s) 431
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 228
BstZI CGGCCG 1 cut(s) 184
BsuI GTATCC 1 cut(s) 155
BsuRI GGCC 1 cut(s) 186
BtgZI GCGATG 1 cut(s) 93
BtrI CACGTC 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 1 cut(s) 440
Cac8I GCNNGC 2 cut(s) 92, 431
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 319
CseI GACGC 3 cut(s) 142, 301, 381
Csp6I GTAC 3 cut(s) 103, 238, 250
CviJI RGCY 9 cut(s) 90, 169, 186, 210, 219, 234, 244, 289, 334
CviKI_1 RGCY 9 cut(s) 90, 169, 186, 210, 219, 234, 244, 289, 334
CviQI GTAC 3 cut(s) 103, 238, 250
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
EciI GGCGGA 2 cut(s) 58, 480
Ecl136II GAGCTC 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 319
Eco52I CGGCCG 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 371
Eco91I GGTNACC 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 171
FauI CCCGC 3 cut(s) 431, 437, 438
Fnu4HI GCNGC 2 cut(s) 217, 399
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 2 cut(s) 217, 399
GluI GCNGC 2 cut(s) 217, 399
HaeIII GGCC 1 cut(s) 186
HapII CCGG 4 cut(s) 159, 267, 303, 355
HgaI GACGC 3 cut(s) 142, 301, 381
Hin1I GRCGYC 1 cut(s) 153
HinfI GANTC 1 cut(s) 189
HpaII CCGG 4 cut(s) 159, 267, 303, 355
HphI GGTGA 4 cut(s) 33, 148, 343, 374
Hpy166II GTNNAC 3 cut(s) 51, 103, 258
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 3 cut(s) 51, 103, 258
Hpy99I CGWCG 3 cut(s) 89, 347, 397
HpyAV CCTTC 2 cut(s) 142, 224
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 2 cut(s) 110, 216
HpyF10VI GCNNNNNNNGC 5 cut(s) 216, 295, 398, 435, 462
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 1 cut(s) 153
LmnI GCTCC 2 cut(s) 147, 215
LpnPI CCDG 9 cut(s) 172, 212, 226, 254, 280, 316, 335, 341, 368
Lsp1109I GCAGC 1 cut(s) 228
LweI GCATC 1 cut(s) 97
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 154, 349, 362
MboII GAAGA 2 cut(s) 490, 492
MhlI GDGCHC 2 cut(s) 171, 431
MluCI AATT 2 cut(s) 119, 415
MmeI TCCRAC 1 cut(s) 429
MnlI CCTC 9 cut(s) 38, 44, 76, 203, 304, 340, 353, 380, 501
MseI TTAA 1 cut(s) 483
MspI CCGG 4 cut(s) 159, 267, 303, 355
MspR9I CCNGG 2 cut(s) 304, 355
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 5 cut(s) 216, 295, 398, 435, 462
NciI CCSGG 2 cut(s) 304, 355
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 3 cut(s) 154, 349, 362
PaeR7I CTCGAG 1 cut(s) 371
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 81, 342
PfeI GAWTC 1 cut(s) 189
PflFI GACNNNGTC 1 cut(s) 341
PkrI GCNGC 2 cut(s) 218, 400
PpuMI RGGWCCY 1 cut(s) 319
PshAI GACNNNNGTC 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 319
PspEI GGTNACC 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 319
PspPPI RGGWCCY 1 cut(s) 319
PspXI VCTCGAGB 1 cut(s) 371
PstI CTGCAG 1 cut(s) 218
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 3 cut(s) 104, 239, 251
RsaNI GTAC 3 cut(s) 103, 238, 250
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 2 cut(s) 217, 399
Sau96I GGNCC 1 cut(s) 319
ScrFI CCNGG 2 cut(s) 304, 355
SduI GDGCHC 2 cut(s) 171, 431
SetI ASST 9 cut(s) 56, 171, 221, 246, 291, 321, 351, 364, 372
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 214
Sfr274I CTCGAG 1 cut(s) 371
SinI GGWCC 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 371
SmlI CTYRAG 2 cut(s) 245, 371
SmoI CTYRAG 2 cut(s) 245, 371
Sse9I AATT 2 cut(s) 119, 415
SsiI CCGC 7 cut(s) 63, 69, 398, 431, 438, 444, 465
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 302, 353
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 1 cut(s) 56
TaqI TCGA 5 cut(s) 171, 336, 345, 372, 411
TasI AATT 2 cut(s) 119, 415
TatI WGTACW 1 cut(s) 249
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 1 cut(s) 189
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TscAI CASTG 1 cut(s) 440
TseFI GTSAC 3 cut(s) 154, 349, 362
TseI GCWGC 1 cut(s) 216
Tsp45I GTSAC 3 cut(s) 154, 349, 362
TspRI CASTG 1 cut(s) 440
Tth111I GACNNNGTC 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.