Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0003757

Physical Location & Seq

Genomic Coordinates Reverse (-)

38596605 .. 38601614

Gene Structure 5010 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0242500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGGGCTGCGCGAGCTCCAAGCTCGACGACCTGCCGGCGGTGGCGCTCTGCCGCGAGCGGTGTAGCTACCTCGACGAGGCGATTCACCAGCGCTACGCTCTCGCCGAGGCCCACATCGGTTATATTCACTCTCTCCGCGGCATCGGCCAGTCTCTCACTCATTTCATCGAACTAGAGGTCGCCAATTCCTCCGGCTCGCCGCCTTCTCCCACTCTCAACCTCCCTCCGGTCCGCAAAGGCGATCACGTCGCCGCCAAATCCTCCTCCCCGCACCACCTCTCCCACTCTAACTCCGGCTCCCACCTCCAATTCCACTCAGATTCCGACGACGACTCCGTCGGCTCGCTCCACCATTCCGACCACTCCTCGCCTCTGCACCTCCACGGCGGCATGGACTACATGGACGGCGGCGGCGGCCAAGACGACATGAACCCCTACCCGCCGCAGGGGTATACGCACATGAACTACATGAGGAACAAAGCCACGCCGTCTGTGGTGTACCAGCAGAAACCCATGAGCCCCGAGAACGTGTACCATATGGGCGAATCCTCCAACTCATCTTACTACCCCTATCCTTACCAAGACGGCGGCGGCGCCAATCCTTATCCCTATTCCGGGTACTCCAATTACGGCGGTGGAAGTAGCGGTGGGTATTATGGTAATTCCACACCCGCGCCTCCTCCTTACGGGGTGTCCTCGCCGCCGGCTCCCCAGTCAAAGCCACCGCCTCCGCCGCCTTCGCCGCCGAAAGCCTCCGCCTGGGACTTCATAAACCCGTTCGAGAGCTCAAACATTGAGAGCTACTACTCGGCTTACACGCCGAGCCGGGACTCGAGGGAGGTCAGAGAAGAAGAGGGAATTCCCGATTTGGAAGACGAGGAGTACAACAACCACAGCCAGTACCAGCAGGAAGTGGTGAAGGAAGTTCACAGAGAGCACCACAAGGAAGGGAAGCAGCATCATCGTTCTTCGAAAGCCGTGGAGGATGAGGAGGTCGTCGAAGCTCAGGCCTCGCTGTACCAGACGAGGCCGAGCGTCGAGAACGAAAACGGGGTGGAGTATGAGGTCCATGTGGTGGACAAGAAGGTTGTGGATGAGGAGGAGAAGCGTGAGGACCACCGGAGTTCCGGTGGAGGTGGTGGAGCATTCAAGCCGGGTTCTAGGGATGCTTTTGATGTGGCTAGAGAAATTCAGGCTCAGTTTCAGAGAGCTTCCGAGTCGGGTAACGAAATCGCAAGGATGCTGGAGGTGGGAAGGCATCCGTATAATCGAAAACATGTTCCTTCCAAGATGAAATCGCTAGCGTCGTTGCAGCCTTCTACTTCCAACAGTGTTGATGGGGCATCATCCTCCGAGGCTGGTCCTGCTCAATTGGGGTTTGATGAAGAACTGATGATGAGGACTAAAAATCTGTCTTCTACGTTGCACAAGCTCTATCTTTGGGAGAAGAAACTGTACAATGAAGTCAAGGCCGAGGAAAAGATGCGGGTGGTCCATGACAGGAAGTGTCGTAAGCTGAAGCGTTTAGATGAAAGGGGAGCAGAGGCCCAGAAAGTAGATACAACCCGAACTTTGATAAGGAGCTTGTCCACAAAAATAAAAATTGCGATTCAAGTTGTTGACAAGATATCTGTGACCATAAATAAGATCAGGGATGAAGAATTATGGCCACAGTTAAACGAATTAATTCAAGGGTTAACCAGGATGTGGAAATCTATGCTTGAATGCCATCGTACTCAGTGCCTAGCAATCAGAGAAGCCAGGGGTTTAGGTCCTATTGGATCTGGAAAGAAGCTAAGTGATGCTCATGTTGATGCTACATTGCAATTTGAGCATGAGCTTGTTAACTGGACTTTCAGATTCTCTACTTGGATTAGCGCTCAAAAGGGTTATGTGAGAGCTTTGAATAATTGGCTTTTAAAGTGTCTTCTTTATGAACCTGAAGAAACAGCAGATGGAATAGTTCCCTTCTCACCTGGTCGAATAGGTGCGCCTCCTGTTTTTGTAATTTGTAACCAGTGGTCACAAGCTTTGGACAGGATATCAGAAAGAGAGGTGGTTGATTCTATGCGTGTTTTCACCATGAGTGTGCTTCAGAGTTGGGAACAAGGTAAGCTTGAAATGCGGCAGAGAATGACGGAAAATCAAGATCTGCAAAAGAAATTTAAGAACTTGGATAAAAAGGACCAAAAGCTTCAGAAGGAGATTCAAGCATTAGACAAGAAAGTGGTGCTAGTCTCGGGGGATGTGGACTATCCTGGTGGACAGATTGTATATCAAAGTGAGACTAGAAATAGTACTTTACAGGCTAGTCTGCAAAGAATTTTTGAGGCCATGGAAAGGTTTACGGAAGTCTCCACAAAAGCTTACGAGGATCTTTTGCAACGCAGTGAAGAAGATAGGCTTGCAGGGGAGCATGAGAGAGTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF538

Protein Analysis

810

Amino Acids

91.06

Weight (kDa)

5.98

Isoelectric Point (pI)

51.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 58	3.3e-25	Protein of unknown function (DUF630)
DUF632	PF04782	392 - 697	1.9e-111	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003757)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02816 AT4G02370
fragaria_vesca	FvH4_2g25870 FvH4_2g25880
malus_domestica	MD03G1290000.v1.1 MD03G1290100.v1.1 MD03G1290200.v1.1 MD03G1290300.v1.1 MD11G1308200.v1.1 MD11G1308300.v1.1
prunus_persica	Prupe.8G263200_v2.0.a1 Prupe.8G263300_v2.0.a1
pyrus_communis	pycom03g22950 pycom11g26940 pycom11g26950
rosa_chinensis	RchiOBHm_Chr6g0294201 RchiOBHm_Chr6g0294211
rosa_laevigata	RLG00000011890 RLG00000011891
rosa_multiflora	Rmu_co8452345.1_g000001 Rmu_sc0002462.1_g000001 Rmu_sc0002462.1_g000002
rosa_roxburghii	Rroxscaffold_7G00173550
rosa_rugosa	Rorug06G0242400 Rorug06G0242500
rosa_samantha	Rh6AG354600 Rh6AG354700 Rh6BG361500 Rh6BG361600 Rh6CG368800 Rh6CG368900 Rh6DG355600 Rh6DG355700
rosa_wichuraiana	Rw6G030910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	335
Acc36I	ACCTGC	1 cut(s)	39
AccB1I	GGYRCC	1 cut(s)	593
AccB7I	CCANNNNNTGG	2 cut(s)	1075, 1707
AccBSI	CCGCTC	1 cut(s)	58
AccI	GTMKAC	1 cut(s)	452
AccII	CGCG	4 cut(s)	11, 54, 138, 674
AclWI	GGATC	2 cut(s)	1789, 2382
AcoI	YGGCCR	3 cut(s)	145, 415, 1667
AcsI	RAATTY	4 cut(s)	858, 1188, 2162, 2322
AcuI	CTGAAG	4 cut(s)	1538, 1962, 2078, 2180
AcyI	GRCGYC	1 cut(s)	594
AfaI	GTAC	9 cut(s)	500, 533, 620, 884, 902, 1019, 1457, 1735, 2299
AfeI	AGCGCT	2 cut(s)	92, 1879
AflIII	ACRYGT	2 cut(s)	528, 1276
AgsI	TTSAA	7 cut(s)	1150, 1613, 1691, 1724, 1906, 2120, 2210
AjiI	CACGTC	1 cut(s)	247
AjnI	CCWGG	5 cut(s)	758, 1700, 1760, 1975, 2257
Alw21I	GWGCWC	3 cut(s)	17, 788, 939
Alw26I	GTCTC	4 cut(s)	156, 2242, 2279, 2359
AlwI	GGATC	2 cut(s)	1789, 2382
Ama87I	CYCGRG	3 cut(s)	521, 832, 2239
Aor51HI	AGCGCT	2 cut(s)	92, 1879
AoxI	GGCC	9 cut(s)	108, 145, 415, 1008, 1028, 1470, 1545, 1667, 2331
ApeKI	GCWGC	3 cut(s)	6, 955, 1312
ApoI	RAATTY	4 cut(s)	858, 1188, 2162, 2322
ArsI	GACNNNNNNTTYG	2 cut(s)	162, 194
AseI	ATTAAT	1 cut(s)	1685
Asp700I	GAANNNNTTC	1 cut(s)	1686
AspLEI	GCGC	7 cut(s)	11, 46, 93, 596, 676, 1880, 1993
AspS9I	GGNCC	9 cut(s)	109, 229, 1066, 1114, 1361, 1492, 1546, 1772, 2185
AsuC2I	CCSGG	3 cut(s)	616, 827, 1155
AsuHPI	GGTGA	4 cut(s)	77, 928, 1965, 2071
AsuII	TTCGAA	1 cut(s)	971
AsuNHI	GCTAGC	1 cut(s)	1300
AvaI	CYCGRG	3 cut(s)	521, 832, 2239
AvaII	GGWCC	7 cut(s)	229, 1066, 1114, 1361, 1492, 1772, 2185
BalI	TGGCCA	1 cut(s)	1669
BanI	GGYRCC	1 cut(s)	593
BanII	GRGCYC	3 cut(s)	17, 521, 788
BarI	GAAGNNNNNNTAC	2 cut(s)	1439, 1471
BbsI	GAAGAC	3 cut(s)	879, 1407, 1919
Bbv12I	GWGCWC	3 cut(s)	17, 788, 939
BbvI	GCAGC	2 cut(s)	967, 1324
BccI	CCATC	3 cut(s)	1331, 1737, 1949
BceAI	ACGGC	6 cut(s)	400, 421, 472, 601, 646, 962
BcgI	CGANNNNNNTGC	2 cut(s)	944, 978
BciT130I	CCWGG	5 cut(s)	760, 1702, 1762, 1977, 2259
BcnI	CCSGG	3 cut(s)	616, 827, 1155
BcoDI	GTCTC	4 cut(s)	156, 2242, 2279, 2359
BfaI	CTAG	8 cut(s)	173, 1161, 1182, 1301, 1745, 2234, 2289, 2310
BfoI	RGCGCY	4 cut(s)	47, 94, 597, 1881
BfuAI	ACCTGC	1 cut(s)	39
BglII	AGATCT	1 cut(s)	2149
BmcAI	AGTACT	1 cut(s)	2299
Bme1390I	CCNGG	8 cut(s)	616, 760, 827, 1155, 1702, 1762, 1977, 2259
Bme18I	GGWCC	7 cut(s)	229, 1066, 1114, 1361, 1492, 1772, 2185
BmeT110I	CYCGRG	3 cut(s)	521, 832, 2239
BmgBI	CACGTC	1 cut(s)	247
BmgT120I	GGNCC	9 cut(s)	109, 229, 1066, 1114, 1361, 1492, 1546, 1772, 2185
BmiI	GGNNCC	3 cut(s)	298, 595, 708
BmrFI	CCNGG	8 cut(s)	616, 760, 827, 1155, 1702, 1762, 1977, 2259
BmrI	ACTGGG	1 cut(s)	706
BmsI	GCATC	9 cut(s)	150, 967, 1156, 1230, 1267, 1352, 1473, 1792, 1804
BmtI	GCTAGC	1 cut(s)	1304
BmuI	ACTGGG	1 cut(s)	706
BpiI	GAAGAC	3 cut(s)	879, 1407, 1919
BpmI	CTGGAG	1 cut(s)	1265
Bpu10I	CCTNAGC	1 cut(s)	1005
Bpu14I	TTCGAA	1 cut(s)	971
BpuMI	CCSGG	3 cut(s)	616, 827, 1155
BsaHI	GRCGYC	1 cut(s)	594
BsaJI	CCNNGG	9 cut(s)	105, 136, 382, 759, 978, 1353, 1473, 1761, 2334
BsaWI	WCCGGW	3 cut(s)	226, 1119, 1127
Bse118I	RCCGGY	2 cut(s)	34, 703
Bse1I	ACTGG	5 cut(s)	148, 712, 898, 1853, 2017
Bse3DI	GCAATG	1 cut(s)	1820
BseBI	CCWGG	5 cut(s)	760, 1702, 1762, 1977, 2259
BseDI	CCNNGG	9 cut(s)	105, 136, 382, 759, 978, 1353, 1473, 1761, 2334
BseGI	GGATG	9 cut(s)	991, 1099, 1171, 1245, 1258, 1346, 1660, 1710, 2251
BseMI	GCAATG	1 cut(s)	1820
BseMII	CTCAG	4 cut(s)	330, 1019, 1211, 1751
BseNI	ACTGG	5 cut(s)	148, 712, 898, 1853, 2017
BseRI	GAGGAG	7 cut(s)	253, 355, 669, 893, 1004, 1112, 1115
BseXI	GCAGC	2 cut(s)	967, 1324
BsgI	GTGCAG	1 cut(s)	359
Bsh1236I	CGCG	4 cut(s)	11, 54, 138, 674
BshFI	GGCC	9 cut(s)	110, 147, 417, 1010, 1030, 1472, 1547, 1669, 2333
BshNI	GGYRCC	1 cut(s)	593
BsiHKAI	GWGCWC	3 cut(s)	17, 788, 939
BsiHKCI	CYCGRG	3 cut(s)	521, 832, 2239
BslFI	GGGAC	2 cut(s)	776, 842
BsmAI	GTCTC	4 cut(s)	156, 2242, 2279, 2359
BsmFI	GGGAC	2 cut(s)	776, 842
BsmI	GAATGC	2 cut(s)	1145, 1730
BsnI	GGCC	9 cut(s)	110, 147, 417, 1010, 1030, 1472, 1547, 1669, 2333
BsoBI	CYCGRG	3 cut(s)	521, 832, 2239
Bsp119I	TTCGAA	1 cut(s)	971
Bsp1286I	GDGCHC	4 cut(s)	17, 521, 788, 939
Bsp1407I	TGTACA	1 cut(s)	1455
Bsp143I	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
Bsp19I	CCATGG	1 cut(s)	2334
BspANI	GGCC	9 cut(s)	110, 147, 417, 1010, 1030, 1472, 1547, 1669, 2333
BspCNI	CTCAG	4 cut(s)	329, 1018, 1210, 1750
BspFNI	CGCG	4 cut(s)	11, 54, 138, 674
BspLI	GGNNCC	3 cut(s)	298, 595, 708
BspMI	ACCTGC	1 cut(s)	39
BspOI	GCTAGC	1 cut(s)	1304
BspPI	GGATC	2 cut(s)	1789, 2382
BspT104I	TTCGAA	1 cut(s)	971
BspT107I	GGYRCC	1 cut(s)	593
BsrBI	CCGCTC	1 cut(s)	58
BsrDI	GCAATG	1 cut(s)	1820
BsrFI	RCCGGY	2 cut(s)	34, 703
BsrGI	TGTACA	1 cut(s)	1455
BsrI	ACTGG	5 cut(s)	148, 712, 898, 1853, 2017
BssAI	RCCGGY	2 cut(s)	34, 703
BssECI	CCNNGG	9 cut(s)	105, 136, 382, 759, 978, 1353, 1473, 1761, 2334
BssMI	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
BssNAI	GTATAC	1 cut(s)	453
BssNI	GRCGYC	1 cut(s)	594
BssT1I	CCWWGG	1 cut(s)	2334
Bst1107I	GTATAC	1 cut(s)	453
Bst2UI	CCWGG	5 cut(s)	760, 1702, 1762, 1977, 2259
Bst4CI	ACNGT	3 cut(s)	1331, 1455, 1674
Bst6I	CTCTTC	1 cut(s)	846
BstACI	GRCGYC	1 cut(s)	594
BstAUI	TGTACA	1 cut(s)	1455
BstBI	TTCGAA	1 cut(s)	971
BstC8I	GCNNGC	8 cut(s)	13, 36, 56, 197, 344, 705, 1302, 2406
BstDEI	CTNAG	5 cut(s)	316, 1005, 1197, 1737, 1796
BstDSI	CCRYGG	4 cut(s)	136, 382, 978, 2334
BstENI	CCTNNNNNAGG	1 cut(s)	74
BstF5I	GGATG	9 cut(s)	991, 1099, 1171, 1245, 1258, 1346, 1660, 1710, 2251
BstFNI	CGCG	4 cut(s)	11, 54, 138, 674
BstH2I	RGCGCY	4 cut(s)	47, 94, 597, 1881
BstHHI	GCGC	7 cut(s)	11, 46, 93, 596, 676, 1880, 1993
BstKTI	GATC	5 cut(s)	244, 1650, 1784, 2152, 2377
BstMAI	GTCTC	4 cut(s)	156, 2242, 2279, 2359
BstMBI	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
BstMWI	GCNNNNNNNGC	9 cut(s)	12, 144, 414, 733, 739, 742, 1364, 1831, 2122
BstNI	CCWGG	5 cut(s)	760, 1702, 1762, 1977, 2259
BstNSI	RCATGY	1 cut(s)	1280
BstSCI	CCNGG	8 cut(s)	614, 758, 825, 1153, 1700, 1760, 1975, 2257
BstUI	CGCG	4 cut(s)	11, 54, 138, 674
BstV1I	GCAGC	2 cut(s)	967, 1324
BstV2I	GAAGAC	3 cut(s)	879, 1407, 1919
BstX2I	RGATCY	3 cut(s)	1781, 2149, 2374
BstYI	RGATCY	3 cut(s)	1781, 2149, 2374
BstZ17I	GTATAC	1 cut(s)	453
BsuRI	GGCC	9 cut(s)	110, 147, 417, 1010, 1030, 1472, 1547, 1669, 2333
BtgI	CCRYGG	4 cut(s)	136, 382, 978, 2334
BtrI	CACGTC	1 cut(s)	247
BtsCI	GGATG	9 cut(s)	991, 1099, 1171, 1245, 1258, 1346, 1660, 1710, 2251
BtsI	GCAGTG	1 cut(s)	2395
BtsIMutI	CAGTG	4 cut(s)	1336, 1745, 2024, 2395
BveI	ACCTGC	1 cut(s)	39
Cac8I	GCNNGC	8 cut(s)	13, 36, 56, 197, 344, 705, 1302, 2406
CfoI	GCGC	7 cut(s)	11, 46, 93, 596, 676, 1880, 1993
Cfr10I	RCCGGY	2 cut(s)	34, 703
Cfr13I	GGNCC	9 cut(s)	109, 229, 1066, 1114, 1361, 1492, 1546, 1772, 2185
Cfr42I	CCGCGG	1 cut(s)	139
CpoI	CGGWCCG	1 cut(s)	229
CseI	GACGC	2 cut(s)	1024, 1293
CsiI	ACCWGGT	1 cut(s)	1975
Csp6I	GTAC	9 cut(s)	499, 532, 619, 883, 901, 1018, 1456, 1734, 2298
CspI	CGGWCCG	1 cut(s)	229
CviQI	GTAC	9 cut(s)	499, 532, 619, 883, 901, 1018, 1456, 1734, 2298
DdeI	CTNAG	5 cut(s)	316, 1005, 1197, 1737, 1796
DinI	GGCGCC	1 cut(s)	595
DpnI	GATC	5 cut(s)	243, 1649, 1783, 2151, 2376
DpnII	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
DraI	TTTAAA	1 cut(s)	1920
DrdI	GACNNNNNNGTC	1 cut(s)	335
DseDI	GACNNNNNNGTC	1 cut(s)	335
EaeI	YGGCCR	3 cut(s)	145, 415, 1667
Eam1104I	CTCTTC	1 cut(s)	846
EarI	CTCTTC	1 cut(s)	846
EciI	GGCGGA	2 cut(s)	720, 745
Ecl136II	GAGCTC	2 cut(s)	15, 786
Eco130I	CCWWGG	1 cut(s)	2334
Eco147I	AGGCCT	1 cut(s)	1010
Eco24I	GRGCYC	3 cut(s)	17, 521, 788
Eco32I	GATATC	2 cut(s)	1629, 2043
Eco47I	GGWCC	7 cut(s)	229, 1066, 1114, 1361, 1492, 1772, 2185
Eco47III	AGCGCT	2 cut(s)	92, 1879
Eco53kI	GAGCTC	2 cut(s)	15, 786
Eco57I	CTGAAG	4 cut(s)	1538, 1962, 2078, 2180
Eco88I	CYCGRG	3 cut(s)	521, 832, 2239
EcoICRI	GAGCTC	2 cut(s)	15, 786
EcoNI	CCTNNNNNAGG	1 cut(s)	74
EcoO109I	RGGNCCY	1 cut(s)	1772
EcoRI	GAATTC	1 cut(s)	858
EcoRII	CCWGG	5 cut(s)	758, 1700, 1760, 1975, 2257
EcoRV	GATATC	2 cut(s)	1629, 2043
EcoT14I	CCWWGG	1 cut(s)	2334
EcoT38I	GRGCYC	3 cut(s)	17, 521, 788
EgeI	GGCGCC	1 cut(s)	595
EheI	GGCGCC	1 cut(s)	595
ErhI	CCWWGG	1 cut(s)	2334
FalI	AAGNNNNNCTT	4 cut(s)	2100, 2132, 2388, 2420
FaqI	GGGAC	2 cut(s)	776, 842
FauI	CCCGC	4 cut(s)	276, 447, 679, 1479
FauNDI	CATATG	1 cut(s)	537
FblI	GTMKAC	1 cut(s)	452
FokI	GGATG	9 cut(s)	998, 1106, 1178, 1245, 1252, 1333, 1667, 1717, 2258
FriOI	GRGCYC	3 cut(s)	17, 521, 788
FspBI	CTAG	8 cut(s)	173, 1161, 1182, 1301, 1745, 2234, 2289, 2310
GlaI	GCGC	7 cut(s)	10, 45, 92, 595, 675, 1879, 1992
GsuI	CTGGAG	1 cut(s)	1265
HaeII	RGCGCY	4 cut(s)	47, 94, 597, 1881
HaeIII	GGCC	9 cut(s)	110, 147, 417, 1010, 1030, 1472, 1547, 1669, 2333
HgaI	GACGC	2 cut(s)	1024, 1293
HhaI	GCGC	7 cut(s)	11, 46, 93, 596, 676, 1880, 1993
Hin1I	GRCGYC	1 cut(s)	594
Hin6I	GCGC	7 cut(s)	9, 44, 91, 594, 674, 1878, 1991
HinP1I	GCGC	7 cut(s)	9, 44, 91, 594, 674, 1878, 1991
HincII	GTYRAC	3 cut(s)	1621, 1698, 1846
HindII	GTYRAC	3 cut(s)	1621, 1698, 1846
HindIII	AAGCTT	4 cut(s)	2028, 2114, 2192, 2364
HpaI	GTTAAC	2 cut(s)	1698, 1846
HphI	GGTGA	4 cut(s)	77, 928, 1965, 2071
Hpy188III	TCNNGA	5 cut(s)	781, 863, 1039, 1785, 2147
Hpy99I	CGWCG	9 cut(s)	29, 77, 251, 329, 332, 341, 1001, 1040, 1309
HpyCH4III	ACNGT	3 cut(s)	1331, 1455, 1674
HpyCH4IV	ACGT	3 cut(s)	246, 528, 1421
HpyCH4V	TGCA	8 cut(s)	376, 1312, 1426, 1825, 2155, 2317, 2383, 2408
HpyF10VI	GCNNNNNNNGC	9 cut(s)	12, 144, 414, 733, 739, 742, 1364, 1831, 2122
HpyF3I	CTNAG	5 cut(s)	316, 1005, 1197, 1737, 1796
HpySE526I	ACGT	3 cut(s)	246, 528, 1421
Hsp92I	GRCGYC	1 cut(s)	594
HspAI	GCGC	7 cut(s)	9, 44, 91, 594, 674, 1878, 1991
KasI	GGCGCC	1 cut(s)	593
KroI	GCCGGC	2 cut(s)	34, 703
KroNI	GCCGGC	2 cut(s)	36, 705
KspAI	GTTAAC	2 cut(s)	1698, 1846
KspI	CCGCGG	1 cut(s)	139
Kzo9I	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
LmnI	GCTCC	8 cut(s)	20, 302, 351, 712, 1142, 1538, 1581, 2413
Lsp1109I	GCAGC	2 cut(s)	967, 1324
LweI	GCATC	9 cut(s)	150, 967, 1156, 1230, 1267, 1352, 1473, 1792, 1804
MabI	ACCWGGT	1 cut(s)	1975
MaeI	CTAG	8 cut(s)	173, 1161, 1182, 1301, 1745, 2234, 2289, 2310
MaeII	ACGT	3 cut(s)	246, 528, 1421
MaeIII	GTNAC	4 cut(s)	1223, 1633, 2012, 2022
MalI	GATC	5 cut(s)	243, 1649, 1783, 2151, 2376
MbiI	CCGCTC	1 cut(s)	58
MboI	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
MfeI	CAATTG	1 cut(s)	1370
MflI	RGATCY	3 cut(s)	1781, 2149, 2374
MhlI	GDGCHC	4 cut(s)	17, 521, 788, 939
MlsI	TGGCCA	1 cut(s)	1669
MluNI	TGGCCA	1 cut(s)	1669
Mly113I	GGCGCC	1 cut(s)	594
MlyI	GAGTC	3 cut(s)	326, 824, 1226
MmeI	TCCRAC	4 cut(s)	348, 381, 576, 1350
Mox20I	TGGCCA	1 cut(s)	1669
MroNI	GCCGGC	2 cut(s)	34, 703
MroXI	GAANNNNTTC	1 cut(s)	1686
MscI	TGGCCA	1 cut(s)	1669
MseI	TTAA	6 cut(s)	1676, 1685, 1697, 1845, 1919, 2166
MslI	CAYNNNNRTG	2 cut(s)	1812, 2087
Msp20I	TGGCCA	1 cut(s)	1669
MspA1I	CMGCKG	1 cut(s)	138
MspR9I	CCNGG	8 cut(s)	616, 760, 827, 1155, 1702, 1762, 1977, 2259
MunI	CAATTG	1 cut(s)	1370
Mva1269I	GAATGC	2 cut(s)	1145, 1730
MvaI	CCWGG	5 cut(s)	760, 1702, 1762, 1977, 2259
MvnI	CGCG	4 cut(s)	11, 54, 138, 674
MwoI	GCNNNNNNNGC	9 cut(s)	12, 144, 414, 733, 739, 742, 1364, 1831, 2122
NaeI	GCCGGC	2 cut(s)	36, 705
NarI	GGCGCC	1 cut(s)	594
NciI	CCSGG	3 cut(s)	616, 827, 1155
NcoI	CCATGG	1 cut(s)	2334
NdeI	CATATG	1 cut(s)	537
NdeII	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
NgoMIV	GCCGGC	2 cut(s)	34, 703
NheI	GCTAGC	1 cut(s)	1300
NlaIV	GGNNCC	3 cut(s)	298, 595, 708
NmeAIII	GCCGAG	5 cut(s)	130, 788, 846, 1056, 1498
NmuCI	GTSAC	2 cut(s)	1633, 2022
NspI	RCATGY	1 cut(s)	1280
NspV	TTCGAA	1 cut(s)	971
PaeR7I	CTCGAG	1 cut(s)	832
PceI	AGGCCT	1 cut(s)	1010
PciI	ACATGT	1 cut(s)	1276
PcsI	WCGNNNNNNNCGW	3 cut(s)	102, 333, 1304
PctI	GAATGC	2 cut(s)	1145, 1730
PdiI	GCCGGC	2 cut(s)	36, 705
PdmI	GAANNNNTTC	1 cut(s)	1686
PfeI	GAWTC	7 cut(s)	82, 320, 545, 1609, 1860, 2063, 2206
PflFI	GACNNNGTC	1 cut(s)	335
PflMI	CCANNNNNTGG	2 cut(s)	1075, 1707
PleI	GAGTC	3 cut(s)	326, 824, 1225
PluTI	GGCGCC	1 cut(s)	597
PpsI	GAGTC	3 cut(s)	326, 824, 1225
PpuMI	RGGWCCY	1 cut(s)	1772
PscI	ACATGT	1 cut(s)	1276
PshBI	ATTAAT	1 cut(s)	1685
Psp124BI	GAGCTC	2 cut(s)	17, 788
Psp5II	RGGWCCY	1 cut(s)	1772
Psp6I	CCWGG	5 cut(s)	758, 1700, 1760, 1975, 2257
PspGI	CCWGG	5 cut(s)	758, 1700, 1760, 1975, 2257
PspN4I	GGNNCC	3 cut(s)	298, 595, 708
PspPI	GGNCC	9 cut(s)	109, 229, 1066, 1114, 1361, 1492, 1546, 1772, 2185
PspPPI	RGGWCCY	1 cut(s)	1772
PspXI	VCTCGAGB	1 cut(s)	832
PsuI	RGATCY	3 cut(s)	1781, 2149, 2374
PsyI	GACNNNGTC	1 cut(s)	335
RsaI	GTAC	9 cut(s)	500, 533, 620, 884, 902, 1019, 1457, 1735, 2299
RsaNI	GTAC	9 cut(s)	499, 532, 619, 883, 901, 1018, 1456, 1734, 2298
RseI	CAYNNNNRTG	2 cut(s)	1812, 2087
Rsr2I	CGGWCCG	1 cut(s)	229
RsrII	CGGWCCG	1 cut(s)	229
SacI	GAGCTC	2 cut(s)	17, 788
SacII	CCGCGG	1 cut(s)	139
SaqAI	TTAA	6 cut(s)	1676, 1685, 1697, 1845, 1919, 2166
Sau3AI	GATC	5 cut(s)	241, 1647, 1781, 2149, 2374
Sau96I	GGNCC	9 cut(s)	109, 229, 1066, 1114, 1361, 1492, 1546, 1772, 2185
ScaI	AGTACT	1 cut(s)	2299
SchI	GAGTC	3 cut(s)	326, 824, 1226
ScrFI	CCNGG	8 cut(s)	616, 760, 827, 1155, 1702, 1762, 1977, 2259
SduI	GDGCHC	4 cut(s)	17, 521, 788, 939
SexAI	ACCWGGT	1 cut(s)	1975
SfaNI	GCATC	9 cut(s)	150, 967, 1156, 1230, 1267, 1352, 1473, 1792, 1804
SfoI	GGCGCC	1 cut(s)	595
Sfr274I	CTCGAG	1 cut(s)	832
Sfr303I	CCGCGG	1 cut(s)	139
SfuI	TTCGAA	1 cut(s)	971
SgrBI	CCGCGG	1 cut(s)	139
SinI	GGWCC	7 cut(s)	229, 1066, 1114, 1361, 1492, 1772, 2185
SlaI	CTCGAG	1 cut(s)	832
SmiMI	CAYNNNNRTG	2 cut(s)	1812, 2087
SmlI	CTYRAG	1 cut(s)	832
SmoI	CTYRAG	1 cut(s)	832
SseBI	AGGCCT	1 cut(s)	1010
SspDI	GGCGCC	1 cut(s)	593
SspMI	CTAG	8 cut(s)	173, 1161, 1182, 1301, 1745, 2234, 2289, 2310
SstI	GAGCTC	2 cut(s)	17, 788
StuI	AGGCCT	1 cut(s)	1010
StyD4I	CCNGG	8 cut(s)	614, 758, 825, 1153, 1700, 1760, 1975, 2257
StyI	CCWWGG	1 cut(s)	2334
TaaI	ACNGT	3 cut(s)	1331, 1455, 1674
TaiI	ACGT	3 cut(s)	249, 531, 1424
TatI	WGTACW	3 cut(s)	882, 1455, 2297
TfiI	GAWTC	7 cut(s)	82, 320, 545, 1609, 1860, 2063, 2206
Tru1I	TTAA	6 cut(s)	1676, 1685, 1697, 1845, 1919, 2166
Tru9I	TTAA	6 cut(s)	1676, 1685, 1697, 1845, 1919, 2166
TscAI	CASTG	4 cut(s)	1336, 1745, 2024, 2395
TseFI	GTSAC	2 cut(s)	1633, 2022
TseI	GCWGC	3 cut(s)	6, 955, 1312
Tsp45I	GTSAC	2 cut(s)	1633, 2022
TspGWI	ACGGA	4 cut(s)	325, 1251, 2153, 2363
TspRI	CASTG	4 cut(s)	1336, 1745, 2024, 2395
Tth111I	GACNNNGTC	1 cut(s)	335
Van91I	CCANNNNNTGG	2 cut(s)	1075, 1707
VpaK11BI	GGWCC	7 cut(s)	229, 1066, 1114, 1361, 1492, 1772, 2185
VspI	ATTAAT	1 cut(s)	1685
XagI	CCTNNNNNAGG	1 cut(s)	74
XapI	RAATTY	4 cut(s)	858, 1188, 2162, 2322
XceI	RCATGY	1 cut(s)	1280
XcmI	CCANNNNNNNNNTGG	1 cut(s)	490
XhoI	CTCGAG	1 cut(s)	832
XmiI	GTMKAC	1 cut(s)	452
XmnI	GAANNNNTTC	1 cut(s)	1686
XspI	CTAG	8 cut(s)	173, 1161, 1182, 1301, 1745, 2234, 2289, 2310
ZrmI	AGTACT	1 cut(s)	2299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.