MD03G1290100.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003757
Physical Location & Seq
Reverse (-)
36800626 .. 36801104
479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1290100.v1.1.491

Sequence Viewer

Length: 420 bp
ATGATAATGTATGTTCAATTGGCTCAAGTGAGGGAGAAAATGCAGTTATTTTTTATTAATTTAGGGCGTACTCCCCAAAGACGTAGTGAGCTCTCAGTCTACTTGAACAGCACTTGTAGATTTACCATCGACTCGTATAAGCTGAAGTACAAGTCCACCATAACCGGTGTTGTAACCACTGACAAGATCTCTAGCTTGAGTGGCATCGAAGTTAGGGTGCTCTTTCTGTGGCTGAGCCTTGTCTCAGTGACTCGTGACGACGATGAGCTAGAACTCTCGGTCGGCATCGCCTCCGCAAACTTTCCGGTGAGTAATTTCGATGAGAGTCCGACCTGTGGTTGTGGTTTCGACTGTGTGAATGGTAAAAGAATGATTAAGAATCTGGTGTCTGCTCTTTTAGGTCAGTCTTTAGCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

140

Amino Acids

15.55

Weight (kDa)

8.38

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 107 1.9e-20 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 99
AciI CCGC 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 2 cut(s) 70, 149
AfiI CCNNNNNNNGG 1 cut(s) 335
AgeI ACCGGT 1 cut(s) 164
AgsI TTSAA 2 cut(s) 17, 106
AluBI AGCT 5 cut(s) 91, 142, 195, 268, 413
AluI AGCT 5 cut(s) 91, 142, 195, 268, 413
Alw21I GWGCWC 2 cut(s) 93, 222
Alw26I GTCTC 1 cut(s) 247
AseI ATTAAT 1 cut(s) 57
AsiGI ACCGGT 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 319
BanII GRGCYC 1 cut(s) 93
BauI CACGAG 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 93, 222
BccI CCATC 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 2 cut(s) 192, 269
BglII AGATCT 1 cut(s) 186
BlpI GCTNAGC 1 cut(s) 233
BmsI GCATC 2 cut(s) 213, 294
Bpu1102I GCTNAGC 1 cut(s) 233
BpuEI CTTGAG 2 cut(s) 9, 217
BsaWI WCCGGW 2 cut(s) 164, 304
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse118I RCCGGY 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMII CTCAG 3 cut(s) 108, 224, 258
Bsh1285I CGRYCG 1 cut(s) 282
BshTI ACCGGT 1 cut(s) 164
BsiEI CGRYCG 1 cut(s) 282
BsiHKAI GWGCWC 2 cut(s) 93, 222
BsiSI CCGG 2 cut(s) 165, 305
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 247
Bsp1286I GDGCHC 2 cut(s) 93, 222
Bsp143I GATC 1 cut(s) 186
Bsp1720I GCTNAGC 1 cut(s) 233
BspACI CCGC 1 cut(s) 294
BspCNI CTCAG 3 cut(s) 107, 225, 257
BsrFI RCCGGY 1 cut(s) 164
BssAI RCCGGY 1 cut(s) 164
BssMI GATC 1 cut(s) 186
BssSI CACGAG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 353
BstDEI CTNAG 3 cut(s) 94, 233, 244
BstKTI GATC 1 cut(s) 189
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 1 cut(s) 186
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstX2I RGATCY 1 cut(s) 186
BstYI RGATCY 1 cut(s) 186
BtgZI GCGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 177, 252
Cfr10I RCCGGY 1 cut(s) 164
Csp6I GTAC 2 cut(s) 69, 148
CspAI ACCGGT 1 cut(s) 164
CviJI RGCY 8 cut(s) 23, 91, 142, 195, 232, 237, 268, 413
CviKI_1 RGCY 8 cut(s) 23, 91, 142, 195, 232, 237, 268, 413
CviQI GTAC 2 cut(s) 69, 148
DdeI CTNAG 3 cut(s) 94, 233, 244
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 93
FaiI YATR 3 cut(s) 12, 138, 161
FblI GTMKAC 1 cut(s) 99
FriOI GRGCYC 1 cut(s) 93
FspBI CTAG 2 cut(s) 192, 269
HapII CCGG 2 cut(s) 165, 305
HinfI GANTC 4 cut(s) 131, 250, 325, 379
HpaII CCGG 2 cut(s) 165, 305
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 100, 156
Hpy188I TCNGA 1 cut(s) 330
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 2 cut(s) 100, 156
Hpy99I CGWCG 1 cut(s) 263
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 1 cut(s) 43
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
HpyF3I CTNAG 3 cut(s) 94, 233, 244
HpySE526I ACGT 1 cut(s) 82
Kzo9I GATC 1 cut(s) 186
LpnPI CCDG 4 cut(s) 178, 318, 346, 368
LweI GCATC 2 cut(s) 213, 294
MaeI CTAG 2 cut(s) 192, 269
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 3 cut(s) 172, 247, 254
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MfeI CAATTG 1 cut(s) 17
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 2 cut(s) 93, 222
MluCI AATT 3 cut(s) 17, 58, 313
MlyI GAGTC 3 cut(s) 125, 244, 334
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 2 cut(s) 24, 301
MseI TTAA 2 cut(s) 57, 375
MspI CCGG 2 cut(s) 165, 305
MunI CAATTG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 201
NdeII GATC 1 cut(s) 186
NmuCI GTSAC 2 cut(s) 247, 254
PfeI GAWTC 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 164
PleI GAGTC 3 cut(s) 125, 244, 333
PpsI GAGTC 3 cut(s) 125, 244, 333
PshBI ATTAAT 1 cut(s) 57
Psp124BI GAGCTC 1 cut(s) 93
PsuI RGATCY 1 cut(s) 186
RsaI GTAC 2 cut(s) 70, 149
RsaNI GTAC 2 cut(s) 69, 148
SacI GAGCTC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 57, 375
Sau3AI GATC 1 cut(s) 186
SchI GAGTC 3 cut(s) 125, 244, 334
SduI GDGCHC 2 cut(s) 93, 222
SetI ASST 8 cut(s) 85, 93, 144, 197, 270, 335, 403, 415
SfaNI GCATC 2 cut(s) 213, 294
SmlI CTYRAG 2 cut(s) 24, 196
SmoI CTYRAG 2 cut(s) 24, 196
Sse9I AATT 3 cut(s) 17, 58, 313
SsiI CCGC 1 cut(s) 294
SspMI CTAG 2 cut(s) 192, 269
SstI GAGCTC 1 cut(s) 93
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 1 cut(s) 85
TaqI TCGA 4 cut(s) 129, 207, 318, 348
TaqII GACCGA 1 cut(s) 268
TasI AATT 3 cut(s) 17, 58, 313
TatI WGTACW 1 cut(s) 147
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 2 cut(s) 57, 375
Tru9I TTAA 2 cut(s) 57, 375
TscAI CASTG 2 cut(s) 184, 252
TseFI GTSAC 2 cut(s) 247, 254
Tsp45I GTSAC 2 cut(s) 247, 254
TspRI CASTG 2 cut(s) 184, 252
VspI ATTAAT 1 cut(s) 57
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 2 cut(s) 192, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.