pycom03g22950

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003757
Physical Location & Seq
Reverse (-)
22958859 .. 22959533
675 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22950.1

Sequence Viewer

Length: 675 bp
ATGCAGCACCACGTGCCCACTTTCTACGTCGTCGTTTCAACCAACCAAATTTATCTTGTCCACGGCTGTCAGTCAACCCACCTAACCGACTCTCTGTGCCAAACAAAACTATTTTCCCGGAAAAACAAAACAAAAAATTTCGGTGAGAAAAAAAAAAAAGAAAAAAAAATCATGTCTCTCGCAACAATTCTCGCCGTCTTTGTCCTCCTATCGCCGTTCTCCACGTCATCAACCGCCTCCTCCAACGGCGACGGCTCGCGGTCTGTGTACGATGCAATCCAAGACTTCAACTTTCCGATGGGTCTGCTCCCGAAGGGCGTCACCGGATATGAGCTCGACAATGGCAACGGCCGATTCCGCGCGTATTTGAACGGCTCCTGCAGCTTCTCGCTGGAAGGCTCGTACCAGCTCAAGTACAAATCCACCATTACCGGCACAATCTCCCCGAACAAGCTCGCCTCGCTGACCGGGGTCAGCGTCAAGGTCCTGTTCCTGTGGCTCGACATCGTCGAGGTCACCCGGAGCGGCGACAACCTCGAGTTCTCGGTGGGGATCGCATCGGCGGCGTTTCCGATCGACAATTTTTACGAGTGCCCGCAGTGCGGGTGCGGGTTGGATTGCGTCAATGGCGGACAAGTAAGGAAGATTAAAATGCCTCTTGTTTCTTCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

225

Amino Acids

24.47

Weight (kDa)

9.03

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 89 - 195 2e-34 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 525
AccII CGCG 3 cut(s) 259, 360, 362
AciI CCGC 9 cut(s) 234, 259, 358, 525, 563, 596, 603, 609, 630
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 48, 136
AcvI CACGTG 1 cut(s) 13
AcyI GRCGYC 1 cut(s) 318
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 3 cut(s) 269, 404, 416
AfiI CCNNNNNNNGG 1 cut(s) 602
AgsI TTSAA 3 cut(s) 39, 289, 370
AjiI CACGTC 1 cut(s) 225
AluBI AGCT 4 cut(s) 334, 384, 409, 454
AluI AGCT 4 cut(s) 334, 384, 409, 454
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 536
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 2 cut(s) 4, 381
ApoI RAATTY 2 cut(s) 48, 136
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 1 cut(s) 484
AsuC2I CCSGG 3 cut(s) 118, 469, 520
AsuHPI GGTGA 3 cut(s) 155, 313, 508
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 1 cut(s) 484
BaeGI GKGCMC 2 cut(s) 18, 596
BanII GRGCYC 1 cut(s) 336
BbrPI CACGTG 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 2 cut(s) 16, 393
BccI CCATC 1 cut(s) 292
BceAI ACGGC 7 cut(s) 79, 179, 199, 262, 268, 364, 388
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BcnI CCSGG 3 cut(s) 118, 469, 520
BcoDI GTCTC 1 cut(s) 180
BfmI CTRYAG 1 cut(s) 379
BisI GCNGC 4 cut(s) 5, 382, 526, 564
BlsI GCNGC 4 cut(s) 6, 383, 527, 565
Bme1390I CCNGG 3 cut(s) 118, 469, 520
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 536
BmgBI CACGTC 1 cut(s) 225
BmgT120I GGNCC 1 cut(s) 484
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 3 cut(s) 118, 469, 520
BmsI GCATC 2 cut(s) 262, 566
BoxI GACNNNNGTC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 3 cut(s) 118, 469, 520
BsaAI YACGTR 1 cut(s) 13
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 2 cut(s) 61, 468
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse118I RCCGGY 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 61, 468
BseLI CCNNNNNNNGG 1 cut(s) 602
BseRI GAGGAG 1 cut(s) 229
BseSI GKGCMC 2 cut(s) 18, 596
BseX3I CGGCCG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 16, 393
Bsh1236I CGCG 3 cut(s) 259, 360, 362
Bsh1285I CGRYCG 2 cut(s) 352, 576
BshFI GGCC 1 cut(s) 351
BsiEI CGRYCG 2 cut(s) 352, 576
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 5 cut(s) 118, 324, 432, 468, 520
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 1 cut(s) 351
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 3 cut(s) 18, 336, 596
Bsp143I GATC 2 cut(s) 552, 573
BspACI CCGC 9 cut(s) 234, 259, 358, 525, 563, 596, 603, 609, 630
BspANI GGCC 1 cut(s) 351
BspFNI CGCG 3 cut(s) 259, 360, 362
BspLI GGNNCC 1 cut(s) 376
BspMAI CTGCAG 1 cut(s) 383
BspPI GGATC 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 61, 468
BssMI GATC 2 cut(s) 552, 573
BssNI GRCGYC 1 cut(s) 318
BstACI GRCGYC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 13
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 3 cut(s) 257, 456, 596
BstDSI CCRYGG 1 cut(s) 61
BstEII GGTNACC 1 cut(s) 514
BstFNI CGCG 3 cut(s) 259, 360, 362
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 2 cut(s) 555, 576
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 2 cut(s) 552, 573
BstMCI CGRYCG 2 cut(s) 352, 576
BstMWI GCNNNNNNNGC 7 cut(s) 13, 357, 381, 460, 563, 600, 627
BstPAI GACNNNNGTC 1 cut(s) 470
BstPI GGTNACC 1 cut(s) 514
BstSCI CCNGG 3 cut(s) 116, 467, 518
BstSFI CTRYAG 1 cut(s) 379
BstSLI GKGCMC 2 cut(s) 18, 596
BstUI CGCG 3 cut(s) 259, 360, 362
BstV1I GCAGC 2 cut(s) 16, 393
BstZI CGGCCG 1 cut(s) 349
BsuRI GGCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 61
BtrI CACGTC 1 cut(s) 225
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 1 cut(s) 605
Cac8I GCNNGC 3 cut(s) 257, 456, 596
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 484
CseI GACGC 3 cut(s) 307, 466, 610
Csp6I GTAC 3 cut(s) 268, 403, 415
CviAII CATG 1 cut(s) 172
CviQI GTAC 3 cut(s) 268, 403, 415
DpnI GATC 2 cut(s) 554, 575
DpnII GATC 2 cut(s) 552, 573
DraIII CACNNNGTG 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 349
EagI CGGCCG 1 cut(s) 349
EciI GGCGGA 1 cut(s) 645
Ecl136II GAGCTC 1 cut(s) 334
EclXI CGGCCG 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 484
Eco52I CGGCCG 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 334
Eco72I CACGTG 1 cut(s) 13
Eco88I CYCGRG 1 cut(s) 536
Eco91I GGTNACC 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 484
EcoO65I GGTNACC 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 336
FaeI CATG 1 cut(s) 175
FaiI YATR 2 cut(s) 173, 330
FatI CATG 1 cut(s) 171
FauI CCCGC 3 cut(s) 596, 602, 603
Fnu4HI GCNGC 4 cut(s) 5, 382, 526, 564
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 4 cut(s) 5, 382, 526, 564
GlaI GCGC 1 cut(s) 361
GluI GCNGC 4 cut(s) 5, 382, 526, 564
HaeIII GGCC 1 cut(s) 351
HapII CCGG 5 cut(s) 118, 324, 432, 468, 520
HgaI GACGC 3 cut(s) 307, 466, 610
HhaI GCGC 1 cut(s) 362
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 2 cut(s) 89, 354
HpaII CCGG 5 cut(s) 118, 324, 432, 468, 520
HphI GGTGA 3 cut(s) 155, 313, 508
Hpy166II GTNNAC 3 cut(s) 61, 75, 268
Hpy188I TCNGA 2 cut(s) 297, 573
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 3 cut(s) 61, 75, 268
Hpy99I CGWCG 4 cut(s) 32, 35, 254, 512
HpyAV CCTTC 2 cut(s) 307, 389
HpyCH4IV ACGT 3 cut(s) 12, 27, 224
HpyCH4V TGCA 3 cut(s) 4, 275, 381
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 357, 381, 460, 563, 600, 627
HpySE526I ACGT 3 cut(s) 12, 27, 224
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 1 cut(s) 360
Kzo9I GATC 2 cut(s) 552, 573
LmnI GCTCC 3 cut(s) 312, 380, 522
Lsp1109I GCAGC 2 cut(s) 16, 393
LweI GCATC 2 cut(s) 262, 566
MaeII ACGT 3 cut(s) 12, 27, 224
MaeIII GTNAC 2 cut(s) 319, 514
MalI GATC 2 cut(s) 554, 575
MbiI CCGCTC 1 cut(s) 525
MboI GATC 2 cut(s) 552, 573
MboII GAAGA 2 cut(s) 655, 657
MhlI GDGCHC 3 cut(s) 18, 336, 596
MluCI AATT 4 cut(s) 48, 136, 186, 580
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 2 cut(s) 267, 594
MnlI CCTC 7 cut(s) 215, 247, 250, 469, 505, 545, 666
MseI TTAA 1 cut(s) 648
MspI CCGG 5 cut(s) 118, 324, 432, 468, 520
MspR9I CCNGG 3 cut(s) 118, 469, 520
MvnI CGCG 3 cut(s) 259, 360, 362
MwoI GCNNNNNNNGC 7 cut(s) 13, 357, 381, 460, 563, 600, 627
NciI CCSGG 3 cut(s) 118, 469, 520
NdeII GATC 2 cut(s) 552, 573
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 2 cut(s) 319, 514
PaeR7I CTCGAG 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PfeI GAWTC 1 cut(s) 354
PflFI GACNNNGTC 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 4 cut(s) 6, 383, 527, 565
Ple19I CGATCG 1 cut(s) 576
PleI GAGTC 1 cut(s) 83
PmaCI CACGTG 1 cut(s) 13
PmlI CACGTG 1 cut(s) 13
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 484
PshAI GACNNNNGTC 1 cut(s) 470
Psp124BI GAGCTC 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 484
PspCI CACGTG 1 cut(s) 13
PspEI GGTNACC 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 1 cut(s) 484
PspPPI RGGWCCY 1 cut(s) 484
PspXI VCTCGAGB 1 cut(s) 536
PstI CTGCAG 1 cut(s) 383
PsyI GACNNNGTC 1 cut(s) 506
PvuI CGATCG 1 cut(s) 576
RsaI GTAC 3 cut(s) 269, 404, 416
RsaNI GTAC 3 cut(s) 268, 403, 415
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 4 cut(s) 5, 382, 526, 564
Sau3AI GATC 2 cut(s) 552, 573
Sau96I GGNCC 1 cut(s) 484
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 3 cut(s) 118, 469, 520
SduI GDGCHC 3 cut(s) 18, 336, 596
SfaNI GCATC 2 cut(s) 262, 566
SfcI CTRYAG 1 cut(s) 379
Sfr274I CTCGAG 1 cut(s) 536
SinI GGWCC 1 cut(s) 484
SlaI CTCGAG 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 410, 536
SmoI CTYRAG 2 cut(s) 410, 536
Sse9I AATT 4 cut(s) 48, 136, 186, 580
SsiI CCGC 9 cut(s) 234, 259, 358, 525, 563, 596, 603, 609, 630
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 3 cut(s) 116, 467, 518
TaiI ACGT 3 cut(s) 15, 30, 227
TaqI TCGA 6 cut(s) 336, 501, 510, 537, 576, 668
TasI AATT 4 cut(s) 48, 136, 186, 580
TatI WGTACW 1 cut(s) 414
TauI GCSGC 2 cut(s) 528, 566
TfiI GAWTC 1 cut(s) 354
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 1 cut(s) 605
TseFI GTSAC 2 cut(s) 319, 514
TseI GCWGC 2 cut(s) 4, 381
Tsp45I GTSAC 2 cut(s) 319, 514
TspRI CASTG 1 cut(s) 605
Tth111I GACNNNGTC 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 2 cut(s) 48, 136
XhoI CTCGAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.