FvH4_2g32750

Phytosulfokines

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003745
Physical Location & Seq
Forward (+)
24747788 .. 24748466
679 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32750.t1

Sequence Viewer

Length: 258 bp
ATGGCCAAGTTTACCGCCCTTTTCACCATCGCCGCCCTCCTTCTCCTCCTCAGCTCCGAGCTGACCTCCGCGGTTCGCCCGGAGCCCAACAGCGAGCTCAAAACCGTCCAACACCAAGGTGCAGGAGCAGATCAGAACATTGATTTCGAGGATGGCGAGGTTAGCTGCGAGGGAGTGAACGAGGAGGAGTGCCTGATGAGAAGAACCCTGGTTGCTCATGTGGATTACATATATACTCAGAAGCACAAGCACCCTTGA

Protein Analysis

86

Amino Acids

9.46

Weight (kDa)

5.01

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 13 - 84 9.4e-17 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AciI CCGC 4 cut(s) 15, 33, 69, 71
AcoI YGGCCR 1 cut(s) 3
AjnI CCWGG 1 cut(s) 207
AleI CACNNNNGTG 1 cut(s) 117
AluBI AGCT 4 cut(s) 54, 61, 97, 165
AluI AGCT 4 cut(s) 54, 61, 97, 165
Alw21I GWGCWC 1 cut(s) 99
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 165
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 16
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 87, 99
Bbv12I GWGCWC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 50
BbvI GCAGC 1 cut(s) 152
BccI CCATC 2 cut(s) 35, 146
BciT130I CCWGG 1 cut(s) 209
BcnI CCSGG 1 cut(s) 80
BisI GCNGC 2 cut(s) 33, 166
BlsI GCNGC 2 cut(s) 34, 167
Bme1390I CCNGG 2 cut(s) 80, 209
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 80, 209
BplI GAGNNNNNCTC 2 cut(s) 50, 82
Bpu10I CCTNAGC 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 69, 115, 207
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 3 cut(s) 69, 115, 207
BseGI GGATG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 64, 251
BseRI GAGGAG 4 cut(s) 35, 38, 197, 200
BseXI GCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 80
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 87, 99
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 4 cut(s) 15, 33, 69, 71
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 63, 250
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 84
BssECI CCNNGG 3 cut(s) 69, 115, 207
BssMI GATC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 2 cut(s) 50, 237
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 1 cut(s) 133
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 1 cut(s) 162
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 78, 207
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 152
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 1 cut(s) 157
Cac8I GCNNGC 1 cut(s) 95
Cfr42I CCGCGG 1 cut(s) 72
CviAII CATG 1 cut(s) 218
CviJI RGCY 6 cut(s) 5, 54, 61, 85, 97, 165
CviKI_1 RGCY 6 cut(s) 5, 54, 61, 85, 97, 165
DdeI CTNAG 2 cut(s) 50, 237
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 115
Eco24I GRGCYC 2 cut(s) 87, 99
Eco53kI GAGCTC 1 cut(s) 97
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 115
EcoT38I GRGCYC 2 cut(s) 87, 99
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 1 cut(s) 221
FaiI YATR 4 cut(s) 219, 230, 232, 234
FatI CATG 1 cut(s) 217
Fnu4HI GCNGC 2 cut(s) 33, 166
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 2 cut(s) 87, 99
Fsp4HI GCNGC 2 cut(s) 33, 166
GluI GCNGC 2 cut(s) 33, 166
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 80
Hin1II CATG 1 cut(s) 221
HpaII CCGG 1 cut(s) 80
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 12, 178
Hpy188I TCNGA 3 cut(s) 58, 135, 240
Hpy8I GTNNAC 2 cut(s) 12, 178
HpyAV CCTTC 1 cut(s) 50
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 1 cut(s) 162
HpyF3I CTNAG 2 cut(s) 50, 237
Hsp92II CATG 1 cut(s) 221
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 1 cut(s) 130
LmnI GCTCC 3 cut(s) 59, 82, 125
LpnPI CCDG 5 cut(s) 93, 108, 194, 206, 221
Lsp1109I GCAGC 1 cut(s) 152
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 1 cut(s) 213
MhlI GDGCHC 2 cut(s) 87, 99
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 9 cut(s) 47, 56, 59, 76, 142, 151, 163, 175, 178
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 117
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 80, 209
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 162
NciI CCSGG 1 cut(s) 80
NdeII GATC 1 cut(s) 130
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 84
OliI CACNNNNGTG 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PkrI GCNGC 2 cut(s) 34, 167
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 84
RseI CAYNNNNRTG 1 cut(s) 117
SacI GAGCTC 1 cut(s) 99
SacII CCGCGG 1 cut(s) 72
SatI GCNGC 2 cut(s) 33, 166
Sau3AI GATC 1 cut(s) 130
ScrFI CCNGG 2 cut(s) 80, 209
SduI GDGCHC 2 cut(s) 87, 99
SetI ASST 7 cut(s) 56, 63, 68, 99, 121, 162, 167
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 117
SsiI CCGC 4 cut(s) 15, 33, 69, 71
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 78, 207
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 1 cut(s) 147
TauI GCSGC 1 cut(s) 35
TseI GCWGC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.